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P03532

- VP7_ROTHW

UniProt

P03532 - VP7_ROTHW

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Protein
Outer capsid glycoprotein VP7
Gene
N/A
Organism
Rotavirus A (strain Human/United States/Wa/1974 G1-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Outer capsid protein involved in attachment and possibly entry into the host epithelial cell. It is subsequently lost, together with VP4, following virus entry into the host cell. The outer layer contains 780 copies of VP7, grouped as 260 trimers. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In integrin-dependent strains, VP7 seems to essentially target the integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 at a postbinding stage, once the initial attachment by VP4 has been achieved By similarity.

GO - Biological processi

  1. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction

Keywords - Ligandi

Calcium

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid glycoprotein VP7
OrganismiRotavirus A (strain Human/United States/Wa/1974 G1-P1A[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1) (RV-A)
Taxonomic identifieri10962 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000006581: Genome

Subcellular locationi

Virion By similarity. Host endoplasmic reticulum lumen Reviewed prediction
Note: Immature double-layered particles assembled in the cytoplasm bud across the membrane of the endoplasmic reticulum, acquiring during this process a transient lipid membrane that is modified with the ER resident viral glycoproteins NSP4 and VP7; these enveloped particles also contain VP4. As the particles move towards the interior of the ER cisternae, the transient lipid membrane and the non-structural protein NSP4 are lost, while the virus surface proteins VP4 and VP7 rearrange to form the outermost virus protein layer, yielding mature infectious triple-layered particles By similarity.

GO - Cellular componenti

  1. T=13 icosahedral viral capsid Source: UniProtKB-KW
  2. host cell endoplasmic reticulum lumen Source: UniProtKB-SubCell
  3. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host endoplasmic reticulum, Outer capsid protein, T=13 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 5050 Reviewed prediction
Add
BLAST
Chaini51 – 326276Outer capsid glycoprotein VP7 Reviewed prediction
PRO_0000149609Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi69 – 691N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi238 – 2381N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

The N-terminus is blocked possibly by pyroglutamic acid By similarity.
N-glycosylated By similarity.
Intramolecular disulfide bonds By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer; in the presence of calcium By similarity. Acquisition of the capsid outer layer requires a high calcium concentration inside the endoplasmic reticulum. Following cell entry, the low calcium concentration in the cytoplasm is probably responsible for the solubilization of the outer layer. Interacts with host integrin heterodimers ITGAX/ITGB2 and ITGA5/ITGB3 By similarity.

Family & Domainsi

Sequence similaritiesi

Belongs to the rotavirus VP7 family.

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR001963. VP7.
[Graphical view]
PfamiPF00434. VP7. 1 hit.
[Graphical view]
ProDomiPD000191. VP7. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform 1 (identifier: P03532-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MYGIEYTTIL IFLISIILLN YILKSVTRIM DYIIYRFLLI TVALFALTRA    50
QNYGLNLPIT GSMDAVYTNS TQEEVFLTST LCLYYPTEAS TQINDGDWKD 100
SLSQMFLTKG WPTGSVYFKE YSNIVDFSVD PQLYCDYNLV LMKYDQSLEL 150
DMSELADLIL NEWLCNPMDV TLYYYQQSGE SNKWISMGSS CTVKVCPLNT 200
QTLGIGCQTT NVDSFEMIAE NEKLAIVDVV DGINHKINLT TTTCTIRNCK 250
KLGPRENVAV IQVGGSNVLD ITADPTTNPQ TERMMRVNWK KWWQVFYTIV 300
DYINQIVQVM SKRSRSLNSA AFYYRV 326
Length:326
Mass (Da):37,341
Last modified:November 1, 1988 - v1
Checksum:i310963A6223F00D4
GO
Isoform 2 (identifier: P03532-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: Missing.

Note: Produced by alternative initiation at Met-30 of isoform 1. No experimental confirmation available.

Show »
Length:297
Mass (Da):33,941
Checksum:iD5C56B9361D3E45F
GO

Sequence cautioni

The sequence AAA47310.1 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2929Missing in isoform 2.
VSP_038632Add
BLAST

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti149 – 1491E → K in AAA47342. 1 Publication
Sequence conflicti217 – 2171M → N1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02033 Genomic RNA. Translation: AAA47310.1. Different initiation.
M21843 Genomic RNA. Translation: AAA47342.1.
PIRiA04137. VGXR7H.
D27620. VGXRWA.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K02033 Genomic RNA. Translation: AAA47310.1 . Different initiation.
M21843 Genomic RNA. Translation: AAA47342.1 .
PIRi A04137. VGXR7H.
D27620. VGXRWA.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001963. VP7.
[Graphical view ]
Pfami PF00434. VP7. 1 hit.
[Graphical view ]
ProDomi PD000191. VP7. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the gene encoding the serotype-specific antigen of human (Wa) rotavirus: comparison with the homologous genes from simian SA11 and UK bovine rotaviruses."
    Richardson M.A., Iwamoto A., Ikegami N., Nomoto A., Furuichi Y.
    J. Virol. 51:860-862(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Sequence of the serotype-specific glycoprotein of the human rotavirus Wa strain and comparison with other human rotavirus serotypes."
    Mason B.B., Dheer S.K., Hsiao C.-L., Zandle G., Kostek B., Rosanoff E.I., Hung P.P., Davis A.R.
    Virus Res. 2:291-299(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes."
    Green K.Y., Midthun K., Gorziglia M., Hoshino Y., Kapikian A.Z., Chanock R.M., Flores J.
    Virology 161:153-159(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiVP7_ROTHW
AccessioniPrimary (citable) accession number: P03532
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1988
Last modified: February 19, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

In group A rotaviruses, VP7 defines the G serotype.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi