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P03527 (SIGM3_REOVD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Outer capsid protein sigma-3

Short name=Sigma3
Gene names
Name:S4
OrganismReovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3) [Complete proteome]
Taxonomic identifier10886 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostMammalia [TaxID: 40674]

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stimulates translation by blocking the activation of the dsRNA-dependent protein kinase EIF2AK2/PKR, thereby inhibiting the host interferon response. Sigma3 prevents the activation of EIF2AK2 by competing with the kinase for dsRNA-binding. Ref.4

The viral outer shell polypeptides, of which sigma-3 is one, impose structural constraints that prevent elongation of nascent transcripts by the RNA-dependent RNA polymerase lambda-3 By similarity. Ref.4

Subunit structure

Heterohexamer of three sigma-3 and three Mu-1 proteins By similarity. The RNA-binding form is probably a homodimer.

Subcellular location

Virion. Note: Found in the outer capsid. Each subunit is positioned with the small lobe anchoring it to the protein mu1 on the surface of the virion, and the large lobe, the site of initial cleavages during entry-related proteolytic disassembly, protruding outwards.

Post-translational modification

Cleaved during virus the endosomal proteolytic disassembly of the outer capsid.

Sequence similarities

Belongs to the orthoreovirus sigma-3 protein family.

Contains 1 CCHC-type zinc finger.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 365365Outer capsid protein sigma-3
PRO_0000222752

Regions

Zinc finger51 – 7323CCHC-type

Natural variations

Natural variant3541Y → H in strain: Isolate D-EA1 and Isolate D-EA3; altered susceptibility to proteolysis during virus disassembly.

Experimental info

Sequence conflict1331W → R in AAA47283. Ref.1
Sequence conflict1981G → E in AAA47283. Ref.1

Secondary structure

.................................................................... 365
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P03527 [UniParc].

Last modified July 22, 2008. Version 2.
Checksum: CF50174AA43244EB

FASTA36541,118
        10         20         30         40         50         60 
MEVCLPNGHQ VVDLINNAFE GRVSIYSAQE GWDKTISAQP DMMVCGGAVV CMHCLGVVGS 

        70         80         90        100        110        120 
LQRKLKHLPH HRCNQQIRHQ DYVDVQFADR VTAHWKRGML SFVAQMHEMM NDVSPDDLDR 

       130        140        150        160        170        180 
VRTEGGSLVE LNWLQVDPNS MFRSIHSSWT DPLQVVDDLD TKLDQYWTAL NLMIDSSDLI 

       190        200        210        220        230        240 
PNFMMRDPSH AFNGVKLGGD ARQTQFSRTF DSRSSLEWGV MVYDYSELEH DPSKGRAYRK 

       250        260        270        280        290        300 
ELVTPARDFG HFGLSHYSRA TTPILGKMPA VFSGMLTGNC KMYPFIKGTA KLKTVRKLVE 

       310        320        330        340        350        360 
AVNHAWGVEK IRYALGPGGM TGWYNRTMQQ APIVLTPAAL TMFPDTIKFG DLNYPVMIGD 


PMILG 

« Hide

References

[1]"Reovirus type 3 genome segment S4: nucleotide sequence of the gene encoding a major virion surface protein."
Giantini M., Seliger L.S., Furuichi Y., Shatkin A.J.
J. Virol. 52:984-987(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Adaptation of reovirus to growth in the presence of protease inhibitor E64 segregates with a mutation in the carboxy terminus of viral outer-capsid protein sigma3."
Ebert D.H., Wetzel J.D., Brumbaugh D.E., Chance S.R., Stobie L.E., Baer G.S., Dermody T.S.
J. Virol. 75:3197-3206(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Isolate D-EA1 and Isolate D-EA3.
[3]"A plasmid-based reverse genetics system for animal double-stranded RNA viruses."
Kobayashi T., Antar A.A., Boehme K.W., Danthi P., Eby E.A., Guglielmi K.M., Holm G.H., Johnson E.M., Maginnis M.S., Naik S., Skelton W.B., Wetzel J.D., Wilson G.J., Chappell J.D., Dermody T.S.
Cell Host Microbe 1:147-157(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Infectious clone.
[4]"Double-stranded RNA-dependent protein kinase (PKR) is regulated by reovirus structural proteins."
Yue Z., Shatkin A.J.
Virology 234:364-371(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Structure of the reovirus outer capsid and dsRNA-binding protein sigma3 at 1.8 A resolution."
Olland A.M., Jane-Valbuena J., Schiff L.A., Nibert M.L., Harrison S.C.
EMBO J. 20:979-989(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), ZINC-FINGER.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K02739 Genomic RNA. Translation: AAA47283.1.
AF332137 Genomic RNA. Translation: AAK07648.1.
EF494444 Genomic RNA. Translation: ABP48922.1.
PIRMNXRS3. A04127.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1FN9X-ray1.80A/B1-365[»]
ProteinModelPortalP03527.
SMRP03527. Positions 1-365.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000153. Reo_capsid_sigma3.
IPR023634. Reovirus_capsid_sigma-3_dom.
[Graphical view]
PfamPF00979. Reovirus_cap. 1 hit.
[Graphical view]
SUPFAMSSF64465. SSF64465. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP03527.

Entry information

Entry nameSIGM3_REOVD
AccessionPrimary (citable) accession number: P03527
Secondary accession number(s): A4ZY29, Q99AV2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 22, 2008
Last modified: February 19, 2014
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references