Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P03527

- SIGM3_REOVD

UniProt

P03527 - SIGM3_REOVD

Protein

Outer capsid protein sigma-3

Gene

S4

Organism
Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 2 (22 Jul 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Stimulates translation by blocking the activation of the dsRNA-dependent protein kinase EIF2AK2/PKR, thereby inhibiting the host interferon response. Sigma3 prevents the activation of EIF2AK2 by competing with the kinase for dsRNA-binding.1 Publication
    The viral outer shell polypeptides, of which sigma-3 is one, impose structural constraints that prevent elongation of nascent transcripts by the RNA-dependent RNA polymerase lambda-3.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri51 – 7323CCHC-typeAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. RNA binding Source: UniProtKB-KW
    3. structural molecule activity Source: InterPro

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. regulation of translation Source: UniProtKB-KW
    3. suppression by virus of host PKR activity Source: UniProtKB-KW
    4. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
    5. transcription, DNA-templated Source: UniProtKB-KW
    6. viral life cycle Source: InterPro

    Keywords - Biological processi

    Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host PKR by virus, Interferon antiviral system evasion, Transcription, Transcription regulation, Translation regulation, Viral immunoevasion

    Keywords - Ligandi

    Metal-binding, RNA-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Outer capsid protein sigma-3
    Short name:
    Sigma3
    Gene namesi
    Name:S4
    OrganismiReovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
    Taxonomic identifieri10886 [NCBI]
    Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
    Virus hostiMammalia [TaxID: 40674]
    ProteomesiUP000006373: Genome

    Subcellular locationi

    Virion
    Note: Found in the outer capsid. Each subunit is positioned with the small lobe anchoring it to the protein mu1 on the surface of the virion, and the large lobe, the site of initial cleavages during entry-related proteolytic disassembly, protruding outwards.

    GO - Cellular componenti

    1. viral outer capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Outer capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 365365Outer capsid protein sigma-3PRO_0000222752Add
    BLAST

    Post-translational modificationi

    Cleaved during virus the endosomal proteolytic disassembly of the outer capsid.

    Interactioni

    Subunit structurei

    Heterohexamer of three sigma-3 and three Mu-1 proteins By similarity. The RNA-binding form is probably a homodimer.By similarity

    Structurei

    Secondary structure

    1
    365
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi8 – 1811
    Turni19 – 213
    Beta strandi25 – 273
    Turni28 – 303
    Beta strandi41 – 455
    Beta strandi48 – 514
    Turni52 – 543
    Beta strandi57 – 604
    Helixi61 – 633
    Helixi79 – 11133
    Helixi115 – 12410
    Beta strandi126 – 1305
    Helixi133 – 1353
    Turni141 – 1433
    Helixi159 – 17618
    Beta strandi180 – 1867
    Helixi189 – 1924
    Helixi199 – 2035
    Beta strandi221 – 2233
    Helixi226 – 2305
    Helixi232 – 2343
    Helixi236 – 2427
    Helixi246 – 2494
    Helixi252 – 2543
    Beta strandi259 – 2613
    Beta strandi270 – 2723
    Helixi275 – 2773
    Beta strandi282 – 2843
    Helixi288 – 2925
    Helixi293 – 2953
    Helixi296 – 30510
    Helixi308 – 3158
    Helixi319 – 33416
    Helixi338 – 3414
    Beta strandi351 – 3544
    Beta strandi356 – 3583
    Beta strandi362 – 3654

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1FN9X-ray1.80A/B1-365[»]
    ProteinModelPortaliP03527.
    SMRiP03527. Positions 1-365.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03527.

    Family & Domainsi

    Sequence similaritiesi

    Contains 1 CCHC-type zinc finger.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri51 – 7323CCHC-typeAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    InterProiIPR000153. Reo_capsid_sigma3.
    IPR023634. Reovirus_capsid_sigma-3_dom.
    [Graphical view]
    PfamiPF00979. Reovirus_cap. 1 hit.
    [Graphical view]
    SUPFAMiSSF64465. SSF64465. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P03527-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVCLPNGHQ VVDLINNAFE GRVSIYSAQE GWDKTISAQP DMMVCGGAVV    50
    CMHCLGVVGS LQRKLKHLPH HRCNQQIRHQ DYVDVQFADR VTAHWKRGML 100
    SFVAQMHEMM NDVSPDDLDR VRTEGGSLVE LNWLQVDPNS MFRSIHSSWT 150
    DPLQVVDDLD TKLDQYWTAL NLMIDSSDLI PNFMMRDPSH AFNGVKLGGD 200
    ARQTQFSRTF DSRSSLEWGV MVYDYSELEH DPSKGRAYRK ELVTPARDFG 250
    HFGLSHYSRA TTPILGKMPA VFSGMLTGNC KMYPFIKGTA KLKTVRKLVE 300
    AVNHAWGVEK IRYALGPGGM TGWYNRTMQQ APIVLTPAAL TMFPDTIKFG 350
    DLNYPVMIGD PMILG 365
    Length:365
    Mass (Da):41,118
    Last modified:July 22, 2008 - v2
    Checksum:iCF50174AA43244EB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti133 – 1331W → R in AAA47283. (PubMed:6492267)Curated
    Sequence conflicti198 – 1981G → E in AAA47283. (PubMed:6492267)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti354 – 3541Y → H in strain: Isolate D-EA1 and Isolate D-EA3; altered susceptibility to proteolysis during virus disassembly.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02739 Genomic RNA. Translation: AAA47283.1.
    AF332137 Genomic RNA. Translation: AAK07648.1.
    EF494444 Genomic RNA. Translation: ABP48922.1.
    PIRiA04127. MNXRS3.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K02739 Genomic RNA. Translation: AAA47283.1 .
    AF332137 Genomic RNA. Translation: AAK07648.1 .
    EF494444 Genomic RNA. Translation: ABP48922.1 .
    PIRi A04127. MNXRS3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1FN9 X-ray 1.80 A/B 1-365 [» ]
    ProteinModelPortali P03527.
    SMRi P03527. Positions 1-365.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P03527.

    Family and domain databases

    InterProi IPR000153. Reo_capsid_sigma3.
    IPR023634. Reovirus_capsid_sigma-3_dom.
    [Graphical view ]
    Pfami PF00979. Reovirus_cap. 1 hit.
    [Graphical view ]
    SUPFAMi SSF64465. SSF64465. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Reovirus type 3 genome segment S4: nucleotide sequence of the gene encoding a major virion surface protein."
      Giantini M., Seliger L.S., Furuichi Y., Shatkin A.J.
      J. Virol. 52:984-987(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Adaptation of reovirus to growth in the presence of protease inhibitor E64 segregates with a mutation in the carboxy terminus of viral outer-capsid protein sigma3."
      Ebert D.H., Wetzel J.D., Brumbaugh D.E., Chance S.R., Stobie L.E., Baer G.S., Dermody T.S.
      J. Virol. 75:3197-3206(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Isolate D-EA1 and Isolate D-EA3.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: Infectious clone.
    4. "Double-stranded RNA-dependent protein kinase (PKR) is regulated by reovirus structural proteins."
      Yue Z., Shatkin A.J.
      Virology 234:364-371(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Structure of the reovirus outer capsid and dsRNA-binding protein sigma3 at 1.8 A resolution."
      Olland A.M., Jane-Valbuena J., Schiff L.A., Nibert M.L., Harrison S.C.
      EMBO J. 20:979-989(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), ZINC-FINGER.

    Entry informationi

    Entry nameiSIGM3_REOVD
    AccessioniPrimary (citable) accession number: P03527
    Secondary accession number(s): A4ZY29, Q99AV2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 22, 2008
    Last modified: October 1, 2014
    This is version 73 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3