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P03527

- SIGM3_REOVD

UniProt

P03527 - SIGM3_REOVD

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Protein

Outer capsid protein sigma-3

Gene

S4

Organism
Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Stimulates translation by blocking the activation of the dsRNA-dependent protein kinase EIF2AK2/PKR, thereby inhibiting the host interferon response. Sigma3 prevents the activation of EIF2AK2 by competing with the kinase for dsRNA-binding.1 Publication
The viral outer shell polypeptides, of which sigma-3 is one, impose structural constraints that prevent elongation of nascent transcripts by the RNA-dependent RNA polymerase lambda-3.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri51 – 7323CCHC-typeAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. structural molecule activity Source: InterPro

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. regulation of translation Source: UniProtKB-KW
  3. suppression by virus of host PKR activity Source: UniProtKB-KW
  4. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  5. transcription, DNA-templated Source: UniProtKB-KW
  6. viral life cycle Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host PKR by virus, Interferon antiviral system evasion, Transcription, Transcription regulation, Translation regulation, Viral immunoevasion

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Outer capsid protein sigma-3
Short name:
Sigma3
Gene namesi
Name:S4
OrganismiReovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3)
Taxonomic identifieri10886 [NCBI]
Taxonomic lineageiVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostiMammalia [TaxID: 40674]
ProteomesiUP000006373: Genome

Subcellular locationi

Virion
Note: Found in the outer capsid. Each subunit is positioned with the small lobe anchoring it to the protein mu1 on the surface of the virion, and the large lobe, the site of initial cleavages during entry-related proteolytic disassembly, protruding outwards.

GO - Cellular componenti

  1. viral outer capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Outer capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Outer capsid protein sigma-3PRO_0000222752Add
BLAST

Post-translational modificationi

Cleaved during virus the endosomal proteolytic disassembly of the outer capsid.

Interactioni

Subunit structurei

Heterohexamer of three sigma-3 and three Mu-1 proteins (By similarity). The RNA-binding form is probably a homodimer.By similarity

Structurei

Secondary structure

1
365
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi8 – 1811Combined sources
Turni19 – 213Combined sources
Beta strandi25 – 273Combined sources
Turni28 – 303Combined sources
Beta strandi41 – 455Combined sources
Beta strandi48 – 514Combined sources
Turni52 – 543Combined sources
Beta strandi57 – 604Combined sources
Helixi61 – 633Combined sources
Helixi79 – 11133Combined sources
Helixi115 – 12410Combined sources
Beta strandi126 – 1305Combined sources
Helixi133 – 1353Combined sources
Turni141 – 1433Combined sources
Helixi159 – 17618Combined sources
Beta strandi180 – 1867Combined sources
Helixi189 – 1924Combined sources
Helixi199 – 2035Combined sources
Beta strandi221 – 2233Combined sources
Helixi226 – 2305Combined sources
Helixi232 – 2343Combined sources
Helixi236 – 2427Combined sources
Helixi246 – 2494Combined sources
Helixi252 – 2543Combined sources
Beta strandi259 – 2613Combined sources
Beta strandi270 – 2723Combined sources
Helixi275 – 2773Combined sources
Beta strandi282 – 2843Combined sources
Helixi288 – 2925Combined sources
Helixi293 – 2953Combined sources
Helixi296 – 30510Combined sources
Helixi308 – 3158Combined sources
Helixi319 – 33416Combined sources
Helixi338 – 3414Combined sources
Beta strandi351 – 3544Combined sources
Beta strandi356 – 3583Combined sources
Beta strandi362 – 3654Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FN9X-ray1.80A/B1-365[»]
ProteinModelPortaliP03527.
SMRiP03527. Positions 1-365.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03527.

Family & Domainsi

Sequence similaritiesi

Contains 1 CCHC-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri51 – 7323CCHC-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Family and domain databases

InterProiIPR000153. Reo_capsid_sigma3.
IPR023634. Reovirus_capsid_sigma-3_dom.
[Graphical view]
PfamiPF00979. Reovirus_cap. 1 hit.
[Graphical view]
SUPFAMiSSF64465. SSF64465. 1 hit.

Sequencei

Sequence statusi: Complete.

P03527-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEVCLPNGHQ VVDLINNAFE GRVSIYSAQE GWDKTISAQP DMMVCGGAVV
60 70 80 90 100
CMHCLGVVGS LQRKLKHLPH HRCNQQIRHQ DYVDVQFADR VTAHWKRGML
110 120 130 140 150
SFVAQMHEMM NDVSPDDLDR VRTEGGSLVE LNWLQVDPNS MFRSIHSSWT
160 170 180 190 200
DPLQVVDDLD TKLDQYWTAL NLMIDSSDLI PNFMMRDPSH AFNGVKLGGD
210 220 230 240 250
ARQTQFSRTF DSRSSLEWGV MVYDYSELEH DPSKGRAYRK ELVTPARDFG
260 270 280 290 300
HFGLSHYSRA TTPILGKMPA VFSGMLTGNC KMYPFIKGTA KLKTVRKLVE
310 320 330 340 350
AVNHAWGVEK IRYALGPGGM TGWYNRTMQQ APIVLTPAAL TMFPDTIKFG
360
DLNYPVMIGD PMILG
Length:365
Mass (Da):41,118
Last modified:July 22, 2008 - v2
Checksum:iCF50174AA43244EB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti133 – 1331W → R in AAA47283. (PubMed:6492267)Curated
Sequence conflicti198 – 1981G → E in AAA47283. (PubMed:6492267)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti354 – 3541Y → H in strain: Isolate D-EA1 and Isolate D-EA3; altered susceptibility to proteolysis during virus disassembly.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02739 Genomic RNA. Translation: AAA47283.1.
AF332137 Genomic RNA. Translation: AAK07648.1.
EF494444 Genomic RNA. Translation: ABP48922.1.
PIRiA04127. MNXRS3.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02739 Genomic RNA. Translation: AAA47283.1 .
AF332137 Genomic RNA. Translation: AAK07648.1 .
EF494444 Genomic RNA. Translation: ABP48922.1 .
PIRi A04127. MNXRS3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1FN9 X-ray 1.80 A/B 1-365 [» ]
ProteinModelPortali P03527.
SMRi P03527. Positions 1-365.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P03527.

Family and domain databases

InterProi IPR000153. Reo_capsid_sigma3.
IPR023634. Reovirus_capsid_sigma-3_dom.
[Graphical view ]
Pfami PF00979. Reovirus_cap. 1 hit.
[Graphical view ]
SUPFAMi SSF64465. SSF64465. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Reovirus type 3 genome segment S4: nucleotide sequence of the gene encoding a major virion surface protein."
    Giantini M., Seliger L.S., Furuichi Y., Shatkin A.J.
    J. Virol. 52:984-987(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Adaptation of reovirus to growth in the presence of protease inhibitor E64 segregates with a mutation in the carboxy terminus of viral outer-capsid protein sigma3."
    Ebert D.H., Wetzel J.D., Brumbaugh D.E., Chance S.R., Stobie L.E., Baer G.S., Dermody T.S.
    J. Virol. 75:3197-3206(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate D-EA1 and Isolate D-EA3.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Infectious clone.
  4. "Double-stranded RNA-dependent protein kinase (PKR) is regulated by reovirus structural proteins."
    Yue Z., Shatkin A.J.
    Virology 234:364-371(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Structure of the reovirus outer capsid and dsRNA-binding protein sigma3 at 1.8 A resolution."
    Olland A.M., Jane-Valbuena J., Schiff L.A., Nibert M.L., Harrison S.C.
    EMBO J. 20:979-989(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), ZINC-FINGER.

Entry informationi

Entry nameiSIGM3_REOVD
AccessioniPrimary (citable) accession number: P03527
Secondary accession number(s): A4ZY29, Q99AV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 22, 2008
Last modified: November 26, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3