P03526 (SIGNS_REOVD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein sigma-NS
Short name=SigmaNS

Gene names

Name:

S3

Organism

Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3) [Complete proteome]

Taxonomic identifier

10886 [NCBI]

Taxonomic lineage

Viruses › dsRNA viruses › Reoviridae › Spinareovirinae › Orthoreovirus › Mammalian orthoreovirus › Mammalian orthoreovirus 3

Virus host

Mammalia [TaxID: 40674]

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Protein that binds to ssRNA and may be involved in genome packaging By similarity. Ref.2
Subunit structure	Homomultimer By similarity. Interacts with protein mu-NS; in viral inclusions By similarity.
Sequence similarities	Belongs to the orthoreovirus sigma-NS protein family.

Ontologies

Keywords
Ligand	RNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro single-stranded RNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Protein sigma-NS		PRO_0000222759
Regions
	Region	1 – 11	11	Important for ssRNA-binding and formation of complexes By similarity
Natural variations
	Natural variant	260	1	M → T in strain: Mutant tsE320.

Sequences

	Sequence		Length	Mass (Da)
	P03526 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 201A6223C4290D7D	FASTA	366	41,057
	10 20 30 40 50 60 MASSLRAAIS KIKRDDVGQQ VCPNYVMLRS SVTTKVVRNV VEYQIRTGGF FSCLAMLRPL 70 80 90 100 110 120 QYAKRERLLG QRNLERISTR DILQTRDLHS LCMPTPDAPM SNHQASTMRE LICSYFKVDH 130 140 150 160 170 180 ADGLKYIPMD ERYSPSSLAR LFTMGMAGLH ITTEPSYKRV PIMHLAADLD CMTLALPYMI 190 200 210 220 230 240 TLDGDTVVPV APTLSAEQLL DDGLKGLACM DISYGCEVDA NSRPAGDQSM DSSRCINELY 250 260 270 280 290 300 CEETAEAICV LKTCLVLNCM QFKLEMDDLA HNAAELDKIQ MMIPFSERVF RMASSFATID 310 320 330 340 350 360 AQCFRFCVMM KDKNLKIDMR ETTRLWTRSA SDDSVATSSL SISLDRGRWV AADASDARLL VFPIRV

References

[1]	"Nucleotide sequence of reovirus genome segment S3, encoding non-structural protein sigma NS." Richardson M.A., Furuichi Y. Nucleic Acids Res. 11:6399-6408(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Reovirus sigmaNS protein is required for nucleation of viral assembly complexes and formation of viral inclusions." Becker M.M., Goral M.I., Hazelton P.R., Baer G.S., Rodgers S.E., Brown E.G., Coombs K.M., Dermody T.S. J. Virol. 75:1459-1475(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: Mutant tsE320.
[3]	"A plasmid-based reverse genetics system for animal double-stranded RNA viruses." Kobayashi T., Antar A.A., Boehme K.W., Danthi P., Eby E.A., Guglielmi K.M., Holm G.H., Johnson E.M., Maginnis M.S., Naik S., Skelton W.B., Wetzel J.D., Wilson G.J., Chappell J.D., Dermody T.S. Cell Host Microbe 1:147-157(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Infectious clone.
[4]	"Sequence at both termini of the 10 genes of reovirus serotype 3 (strain Dearing)." Antczak J.B., Chmelo R.A., Pickup D.J., Joklik W.K. Virology 121:307-319(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-17.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X01627 Genomic RNA. Translation: CAA25768.1. AF076293 mRNA. Translation: AAG02124.1. EF494443 Genomic RNA. Translation: ABP48921.1. J02330 Genomic RNA. Translation: AAA47280.1.
PIR	MNXRSD. A04126. C27401.
RefSeq	NP_694688.1. NC_004283.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	956478.
Family and domain databases
InterPro	IPR002507. Reovirus_polyG_pol. [Graphical view]
Pfam	PF01518. PolyG_pol. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SIGNS_REOVD

Accession

Primary (citable) accession number: P03526
Secondary accession number(s): A4ZY28, Q9ENN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program