ID   SIGM2_REOVD             Reviewed;         418 AA.
AC   P03525; Q6LEC8;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 2.
DT   24-JAN-2024, entry version 94.
DE   RecName: Full=Inner capsid protein sigma-2;
DE            Short=Sigma2;
GN   Name=S2;
OS   Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Spinareoviridae; Orthoreovirus; Mammalian orthoreovirus.
OX   NCBI_TaxID=10886;
OH   NCBI_TaxID=40674; Mammalia.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Mutant ts447;
RX   PubMed=2718385; DOI=10.1016/0042-6822(89)90392-9;
RA   Wiener J.R., McLaughlin T., Joklik W.K.;
RT   "The sequences of the S2 genome segments of reovirus serotype 3 and of the
RT   dsRNA-negative mutant ts447.";
RL   Virology 170:340-341(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=6961439; DOI=10.1073/pnas.79.24.7644;
RA   Cashdollar L.W., Esparza J., Hudson G.R., Chmelo R.A., Lee P.W.K.,
RA   Joklik W.K.;
RT   "Cloning the double-stranded RNA genes of reovirus: sequence of the cloned
RT   S2 gene.";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:7644-7648(1982).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1920614; DOI=10.1128/jvi.65.11.5721-5731.1991;
RA   Dermody T.S., Schiff L.A., Nibert M.L., Coombs K.M., Fields B.N.;
RT   "The S2 gene nucleotide sequences of prototype strains of the three
RT   reovirus serotypes: characterization of reovirus core protein sigma 2.";
RL   J. Virol. 65:5721-5731(1991).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Infectious clone;
RX   PubMed=18005692; DOI=10.1016/j.chom.2007.03.003;
RA   Kobayashi T., Antar A.A., Boehme K.W., Danthi P., Eby E.A., Guglielmi K.M.,
RA   Holm G.H., Johnson E.M., Maginnis M.S., Naik S., Skelton W.B., Wetzel J.D.,
RA   Wilson G.J., Chappell J.D., Dermody T.S.;
RT   "A plasmid-based reverse genetics system for animal double-stranded RNA
RT   viruses.";
RL   Cell Host Microbe 1:147-157(2007).
CC   -!- FUNCTION: Inner capsid (core) component.
CC   -!- SUBUNIT: Interacts with protein mu-NS; in viral inclusions.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}. Note=Found in the inner
CC       capsid (150 copies).
CC   -!- MISCELLANEOUS: Mutant ts447 is temperature-sensitive.
CC   -!- SIMILARITY: Belongs to the orthoreovirus sigma-1 protein family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=J02327; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; M25780; AAA47279.1; -; Genomic_DNA.
DR   EMBL; J02327; -; NOT_ANNOTATED_CDS; Genomic_RNA.
DR   EMBL; L19776; AAA47261.1; -; Genomic_RNA.
DR   EMBL; EF494442; ABP48920.1; -; Genomic_RNA.
DR   PIR; A31475; FOXR3D.
DR   EMDB; EMD-13149; -.
DR   EMDB; EMD-13150; -.
DR   SMR; P03525; -.
DR   Proteomes; UP000006373; Genome.
DR   Proteomes; UP000165799; Genome.
DR   GO; GO:0039625; C:viral inner capsid; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.287.1520; -; 1.
DR   InterPro; IPR004317; Sigma_1_2_reovir.
DR   Pfam; PF03084; Sigma_1_2; 1.
PE   3: Inferred from homology;
KW   Capsid protein; Inner capsid protein; Virion.
FT   CHAIN           1..418
FT                   /note="Inner capsid protein sigma-2"
FT                   /id="PRO_0000222749"
FT   VARIANT         188
FT                   /note="A -> V (in strain: Mutant ts447;
FT                   temperature-sensitive)"
FT   VARIANT         323
FT                   /note="A -> V (in strain: Mutant ts447;
FT                   temperature-sensitive)"
FT   VARIANT         383
FT                   /note="N -> D (in strain: Mutant ts447;
FT                   temperature-sensitive)"
FT   CONFLICT        259
FT                   /note="H -> Y (in Ref. 2; J02327)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   418 AA;  47161 MW;  0E2F7824DA61D0DC CRC64;
     MARAAFLFKT VGFGGLQNVP INDELSSHLL RAGNSPWQLT QFLDWISLGR GLATSALVPT
     AGSRYYQMSC LLSGTLQIPF RPNHRWGDIR FLRLVWSAPT LDGLVVAPPQ VLAQPALQAQ
     ADRVYDCDDY PFLARDPRFK HRVYQQLSAV TLLNLTGFGP ISYVRVDEDM WSGDVNQLLM
     NYFGHTFAEI AYTLCQASAN RPWEYDGTYA RMTQIVLSLF WLSYVGVIHQ QNTYRTFYFQ
     CNRRGDAAEV WILSCSLNHS AQIRPGNRSL FVMPTSPDWN MDVNLILSST LTGCLCSGSQ
     LPLIDNNSVP AVSRNIHGWT GRAGNQLHGF QVRRMVTEFC DRLRRDGVMT QAQQNQVEAL
     ADQTQQFKRD KLETWAREDD QYNQAHPNST MFRTKPFTNA QWGRGNTGAT SAAIAALI
//