P03525 (SIGM2_REOVD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inner capsid protein sigma-2
Short name=Sigma2

Gene names

Name:

S2

Organism

Reovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3) [Complete proteome]

Taxonomic identifier

10886 [NCBI]

Taxonomic lineage

Viruses › dsRNA viruses › Reoviridae › Spinareovirinae › Orthoreovirus › Mammalian orthoreovirus › Mammalian orthoreovirus 3

Virus host

Mammalia [TaxID: 40674]

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Inner capsid (core) component.
Subunit structure	Interacts with protein mu-NS; in viral inclusions By similarity.
Subcellular location	Virion Potential. Note: Found in the inner capsid (150 copies).
Miscellaneous	Mutant ts447 is temperature-sensitive.
Sequence similarities	Belongs to the orthoreovirus sigma-1 protein family.
Sequence caution	The sequence J02327 differs from that shown. Reason: Frameshift at position 310.

Ontologies

Keywords
Cellular component	Virion
Molecular function	Capsid protein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Inner capsid protein sigma-2		PRO_0000222749
Natural variations
	Natural variant	188	1	A → V in strain: Mutant ts447; temperature-sensitive.
	Natural variant	323	1	A → V in strain: Mutant ts447; temperature-sensitive.
	Natural variant	383	1	N → D in strain: Mutant ts447; temperature-sensitive.
Experimental info
	Sequence conflict	259	1	H → Y in J02327. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P03525 [UniParc]. Last modified August 1, 1990. Version 2. Checksum: 0E2F7824DA61D0DC	FASTA	418	47,161
	10 20 30 40 50 60 MARAAFLFKT VGFGGLQNVP INDELSSHLL RAGNSPWQLT QFLDWISLGR GLATSALVPT 70 80 90 100 110 120 AGSRYYQMSC LLSGTLQIPF RPNHRWGDIR FLRLVWSAPT LDGLVVAPPQ VLAQPALQAQ 130 140 150 160 170 180 ADRVYDCDDY PFLARDPRFK HRVYQQLSAV TLLNLTGFGP ISYVRVDEDM WSGDVNQLLM 190 200 210 220 230 240 NYFGHTFAEI AYTLCQASAN RPWEYDGTYA RMTQIVLSLF WLSYVGVIHQ QNTYRTFYFQ 250 260 270 280 290 300 CNRRGDAAEV WILSCSLNHS AQIRPGNRSL FVMPTSPDWN MDVNLILSST LTGCLCSGSQ 310 320 330 340 350 360 LPLIDNNSVP AVSRNIHGWT GRAGNQLHGF QVRRMVTEFC DRLRRDGVMT QAQQNQVEAL 370 380 390 400 410 ADQTQQFKRD KLETWAREDD QYNQAHPNST MFRTKPFTNA QWGRGNTGAT SAAIAALI

References

[1]	"The sequences of the S2 genome segments of reovirus serotype 3 and of the dsRNA-negative mutant ts447." Wiener J.R., McLaughlin T., Joklik W.K. Virology 170:340-341(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Mutant ts447.
[2]	"Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene." Cashdollar L.W., Esparza J., Hudson G.R., Chmelo R.A., Lee P.W.K., Joklik W.K. Proc. Natl. Acad. Sci. U.S.A. 79:7644-7648(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"The S2 gene nucleotide sequences of prototype strains of the three reovirus serotypes: characterization of reovirus core protein sigma 2." Dermody T.S., Schiff L.A., Nibert M.L., Coombs K.M., Fields B.N. J. Virol. 65:5721-5731(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[4]	"A plasmid-based reverse genetics system for animal double-stranded RNA viruses." Kobayashi T., Antar A.A., Boehme K.W., Danthi P., Eby E.A., Guglielmi K.M., Holm G.H., Johnson E.M., Maginnis M.S., Naik S., Skelton W.B., Wetzel J.D., Wilson G.J., Chappell J.D., Dermody T.S. Cell Host Microbe 1:147-157(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Infectious clone.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M25780 Genomic DNA. Translation: AAA47279.1. J02327 Genomic RNA. No translation available. L19776 Genomic RNA. Translation: AAA47261.1. EF494442 Genomic RNA. Translation: ABP48920.1.
PIR	FOXR3D. A31475.
RefSeq	NP_694684.1. NC_004279.1.
3D structure databases
ProteinModelPortal	P03525.
SMR	P03525. Positions 2-418.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	956473.
Family and domain databases
InterPro	IPR004317. Sigma_1_2_reovir. [Graphical view]
Pfam	PF03084. Sigma_1_2. 1 hit. [Graphical view]
ProDom	PD004419. Sigma_1_2_reovir. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

SIGM2_REOVD

Accession

Primary (citable) accession number: P03525
Secondary accession number(s): Q6LEC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	August 1, 1990
Last modified:	April 3, 2013
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program