Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P03525 (SIGM2_REOVD) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inner capsid protein sigma-2

Short name=Sigma2
Gene names
Name:S2
OrganismReovirus type 3 (strain Dearing) (T3D) (Mammalian orthoreovirus 3) [Complete proteome]
Taxonomic identifier10886 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSpinareovirinaeOrthoreovirus
Virus hostMammalia [TaxID: 40674]

Protein attributes

Sequence length418 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Inner capsid (core) component.

Subunit structure

Interacts with protein mu-NS; in viral inclusions By similarity.

Subcellular location

Virion Potential. Note: Found in the inner capsid (150 copies).

Miscellaneous

Mutant ts447 is temperature-sensitive.

Sequence similarities

Belongs to the orthoreovirus sigma-1 protein family.

Sequence caution

The sequence J02327 differs from that shown. Reason: Frameshift at position 310.

Ontologies

Keywords
   Cellular componentCapsid protein
Inner capsid protein
Virion
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentviral inner capsid

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 418418Inner capsid protein sigma-2
PRO_0000222749

Natural variations

Natural variant1881A → V in strain: Mutant ts447; temperature-sensitive.
Natural variant3231A → V in strain: Mutant ts447; temperature-sensitive.
Natural variant3831N → D in strain: Mutant ts447; temperature-sensitive.

Experimental info

Sequence conflict2591H → Y in J02327. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P03525 [UniParc].

Last modified August 1, 1990. Version 2.
Checksum: 0E2F7824DA61D0DC

FASTA41847,161
        10         20         30         40         50         60 
MARAAFLFKT VGFGGLQNVP INDELSSHLL RAGNSPWQLT QFLDWISLGR GLATSALVPT 

        70         80         90        100        110        120 
AGSRYYQMSC LLSGTLQIPF RPNHRWGDIR FLRLVWSAPT LDGLVVAPPQ VLAQPALQAQ 

       130        140        150        160        170        180 
ADRVYDCDDY PFLARDPRFK HRVYQQLSAV TLLNLTGFGP ISYVRVDEDM WSGDVNQLLM 

       190        200        210        220        230        240 
NYFGHTFAEI AYTLCQASAN RPWEYDGTYA RMTQIVLSLF WLSYVGVIHQ QNTYRTFYFQ 

       250        260        270        280        290        300 
CNRRGDAAEV WILSCSLNHS AQIRPGNRSL FVMPTSPDWN MDVNLILSST LTGCLCSGSQ 

       310        320        330        340        350        360 
LPLIDNNSVP AVSRNIHGWT GRAGNQLHGF QVRRMVTEFC DRLRRDGVMT QAQQNQVEAL 

       370        380        390        400        410 
ADQTQQFKRD KLETWAREDD QYNQAHPNST MFRTKPFTNA QWGRGNTGAT SAAIAALI 

« Hide

References

[1]"The sequences of the S2 genome segments of reovirus serotype 3 and of the dsRNA-negative mutant ts447."
Wiener J.R., McLaughlin T., Joklik W.K.
Virology 170:340-341(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Mutant ts447.
[2]"Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene."
Cashdollar L.W., Esparza J., Hudson G.R., Chmelo R.A., Lee P.W.K., Joklik W.K.
Proc. Natl. Acad. Sci. U.S.A. 79:7644-7648(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]"The S2 gene nucleotide sequences of prototype strains of the three reovirus serotypes: characterization of reovirus core protein sigma 2."
Dermody T.S., Schiff L.A., Nibert M.L., Coombs K.M., Fields B.N.
J. Virol. 65:5721-5731(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[4]"A plasmid-based reverse genetics system for animal double-stranded RNA viruses."
Kobayashi T., Antar A.A., Boehme K.W., Danthi P., Eby E.A., Guglielmi K.M., Holm G.H., Johnson E.M., Maginnis M.S., Naik S., Skelton W.B., Wetzel J.D., Wilson G.J., Chappell J.D., Dermody T.S.
Cell Host Microbe 1:147-157(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Infectious clone.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M25780 Genomic DNA. Translation: AAA47279.1.
J02327 Genomic RNA. No translation available.
L19776 Genomic RNA. Translation: AAA47261.1.
EF494442 Genomic RNA. Translation: ABP48920.1.
PIRFOXR3D. A31475.
RefSeqNP_694684.1. NC_004279.1.

3D structure databases

ProteinModelPortalP03525.
SMRP03525. Positions 2-418.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID956473.

Family and domain databases

InterProIPR004317. Sigma_1_2_reovir.
[Graphical view]
PfamPF03084. Sigma_1_2. 1 hit.
[Graphical view]
ProDomPD004419. Sigma_1_2_reovir. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameSIGM2_REOVD
AccessionPrimary (citable) accession number: P03525
Secondary accession number(s): Q6LEC8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 1, 1990
Last modified: July 9, 2014
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families