P03524 (VGLG_RABVE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycoprotein G

Gene names

Name:

G

Organism

Rabies virus (strain ERA) (RABV) [Complete proteome]

Taxonomic identifier

11295 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Lyssavirus › Rabies virus

Virus host

Homo sapiens (Human) [TaxID: 9606]
Mammalia [TaxID: 40674]

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Attaches the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein G and thereby facilitate rabies virus entry into cells.
Subunit structure	Homotrimer. Interacts with matrix protein By similarity. Ref.7
Subcellular location	Virion membrane; Single-pass type I membrane protein Potential.
Post-translational modification	Glycosylated and palmitoylated by host. Glycosylation is crucial for glycoprotein export at the cell surface. Ref.5 Ref.6
Biotechnological use	Primary surface antigen capable of inducing and reacting with virus-neutralizing antibodies. Almost all human and veterinary vaccines are based on the functional aspects of the G protein.
Miscellaneous	Arg-352 is highly involved in rabies virus pathogenicity. Its mutation dramatically attenuates the virus.
Sequence similarities	Belongs to the lyssavirus glycoprotein family.

Ontologies

Keywords
Cellular component	Membrane Viral envelope protein Virion
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Chain

20 – 524

505

Glycoprotein G

PRO_0000040993

Regions

Topological domain

20 – 459

440

Virion surface Potential

Transmembrane

460 – 480

21

Helical; Potential

Topological domain

481 – 524

44

Intravirion Potential

Amino acid modifications

Lipidation

480

1

S-palmitoyl cysteine; by host By similarity

Glycosylation

56

1

N-linked (GlcNAc...); by host Ref.5

Glycosylation

266

1

N-linked (GlcNAc...); by host Ref.5

Glycosylation

338

1

N-linked (GlcNAc...); by host Ref.5

Natural variations

Natural variant

27

1

L → P in strain: ERA 2007.

Natural variant

55

1

T → I in strain: CVS-11, RV194-2[F3] and RV231-22.

Natural variant

177

1

K → N in strain: RV194-2[F3].

Natural variant

217

1

K → E in strain: RV231-22.

Natural variant

221 – 224

4

KGSE → NGNK in strain: CVS-11, RV194-2[F3] and RV231-22.

Natural variant

352

1

R → Q in strain: RV194-2[F3].

Secondary structure

1

.

.

.

524

Helix Strand Turn

Details...

Beta strand

521 – 523

3

Sequences

	Sequence		Length	Mass (Da)
	P03524 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: BBA53981C1175880	FASTA	524	58,658
	10 20 30 40 50 60 MVPQALLFVP LLVFPLCFGK FPIYTILDKL GPWSPIDIHH LSCPNNLVVE DEGCTNLSGF 70 80 90 100 110 120 SYMELKVGYI LAIKMNGFTC TGVVTEAETY TNFVGYVTTT FKRKHFRPTP DACRAAYNWK 130 140 150 160 170 180 MAGDPRYEES LHNPYPDYRW LRTVKTTKES LVIISPSVAD LDPYDRSLHS RVFPSGKCSG 190 200 210 220 230 240 VAVSSTYCST NHDYTIWMPE NPRLGMSCDI FTNSRGKRAS KGSETCGFVD ERGLYKSLKG 250 260 270 280 290 300 ACKLKLCGVL GLRLMDGTWV AMQTSNETKW CPPDQLVNLH DFRSDEIEHL VVEELVRKRE 310 320 330 340 350 360 ECLDALESIM TTKSVSFRRL SHLRKLVPGF GKAYTIFNKT LMEADAHYKS VRTWNEILPS 370 380 390 400 410 420 KGCLRVGGRC HPHVNGVFFN GIILGPDGNV LIPEMQSSLL QQHMELLESS VIPLVHPLAD 430 440 450 460 470 480 PSTVFKDGDE AEDFVEVHLP DVHNQVSGVD LGLPNWGKYV LLSAGALTAL MLIIFLMTCC 490 500 510 520 RRVNRSEPTQ HNLRGTGREV SVTPQSGKII SSWESHKSGG ETRL

References

[1]	"Structure of the glycoprotein gene in rabies virus." Anilionis A., Wunner W.H., Curtis P.J. Nature 294:275-278(1981) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Amino acid sequence of the rabies virus glycoprotein deduced from its cloned gene." Anilionis A., Wunner W.H., Curtis P.J. Comp. Immunol. Microbiol. Infect. Dis. 5:27-32(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"Complete nucleotide sequencing of SAD derivatives of attenuated rabies virus vaccine strains." Geue L., Schares S., Schnick C., Kliemt J., Beckert A., Hoffmann B., Freuling C., Marston D., McElhinney L., Fooks A., Zanoni R., Peterhans E., Cox J.H., Mueller T. Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: ERA 2007.
[4]	"Antigenic variants of CVS rabies virus with altered glycosylation sites." Wunner W.H., Dietzschold B., Smith C.L., Lafon M., Golub E. Virology 140:1-12(1985) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 49-59; 169-179; 209-226 AND 337-354. Strain: CVS-11, RV194-2[F3] and RV231-22.
[5]	"N-linked glycosylation of rabies virus glycoprotein. Individual sequons differ in their glycosylation efficiencies and influence on cell surface expression." Shakin-Eshleman S.H., Remaley A.T., Eshleman J.R., Wunner W.H., Spitalnik S.L. J. Biol. Chem. 267:10690-10698(1992) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT ASN-56; ASN-266 AND ASN-338.
[6]	"The role of site-specific N-glycosylation in secretion of soluble forms of rabies virus glycoprotein." Wojczyk B.S., Stwora-Wojczyk M., Shakin-Eshleman S.H., Wunner W.H., Spitalnik S.L. Glycobiology 8:121-130(1998) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION.
[7]	"Rabies virus receptors." Lafon M. J. Neurovirol. 11:82-87(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH HOST CELL RECEPTORS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J02293 Genomic RNA. Translation: AAA47204.1. Sequence problems.
M38452 Genomic RNA. Translation: AAA47209.1.
EF206707 Genomic RNA. Translation: ABN11294.1.
K02858 Genomic RNA. Translation: AAA47191.1.
K02859 Genomic RNA. Translation: AAA47192.1.
K02860 Genomic RNA. Translation: AAA47193.1.
K02861 Genomic RNA. Translation: AAA47194.1.
K02862 Genomic RNA. Translation: AAA47195.1.
K02863 Genomic RNA. Translation: AAA47196.1.
K02864 Genomic RNA. Translation: AAA47197.1.
K02865 Genomic RNA. Translation: AAA47198.1.
K02866 Genomic RNA. Translation: AAA47205.1.
K02867 Genomic RNA. Translation: AAA47206.1.
K02868 Genomic RNA. Translation: AAA47207.1.
K02869 Genomic RNA. Translation: AAA47208.1.

PIR

VGVNG. A04121.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3NFK	X-ray	1.43	C/D	512-524	[»]

ProteinModelPortal

P03524.

ModBase

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Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR001903. Rhabd_glycop.
[Graphical view]

Pfam

PF00974. Rhabdo_glycop. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

VGLG_RABVE

Accession

Primary (citable) accession number: P03524
Secondary accession number(s): A3F5L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program