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P03524 (VGLG_RABVE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycoprotein G
Gene names
Name:G
OrganismRabies virus (strain ERA) (RABV) [Complete proteome]
Taxonomic identifier11295 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesMononegaviralesRhabdoviridaeLyssavirus
Virus hostHomo sapiens (Human) [TaxID: 9606]
Mammalia [TaxID: 40674]

Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Attaches the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. There is convincing in vitro evidence that the muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) bind glycoprotein G and thereby facilitate rabies virus entry into cells.

Subunit structure

Homotrimer. Interacts with matrix protein By similarity. Ref.7

Subcellular location

Virion membrane; Single-pass type I membrane protein Potential.

Post-translational modification

Glycosylated and palmitoylated by host. Glycosylation is crucial for glycoprotein export at the cell surface. Ref.5 Ref.6

Biotechnological use

Primary surface antigen capable of inducing and reacting with virus-neutralizing antibodies. Almost all human and veterinary vaccines are based on the functional aspects of the G protein.

Miscellaneous

Arg-352 is highly involved in rabies virus pathogenicity. Its mutation dramatically attenuates the virus.

Sequence similarities

Belongs to the lyssavirus glycoprotein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919
Chain20 – 524505Glycoprotein G
PRO_0000040993

Regions

Topological domain20 – 459440Virion surface Potential
Transmembrane460 – 48021Helical; Potential
Topological domain481 – 52444Intravirion Potential

Amino acid modifications

Lipidation4801S-palmitoyl cysteine; by host By similarity
Glycosylation561N-linked (GlcNAc...); by host Ref.5
Glycosylation2661N-linked (GlcNAc...); by host Ref.5
Glycosylation3381N-linked (GlcNAc...); by host Ref.5

Natural variations

Natural variant271L → P in strain: ERA 2007.
Natural variant551T → I in strain: CVS-11, RV194-2[F3] and RV231-22.
Natural variant1771K → N in strain: RV194-2[F3].
Natural variant2171K → E in strain: RV231-22.
Natural variant221 – 2244KGSE → NGNK in strain: CVS-11, RV194-2[F3] and RV231-22.
Natural variant3521R → Q in strain: RV194-2[F3].

Secondary structure

... 524
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P03524 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: BBA53981C1175880

FASTA52458,658
        10         20         30         40         50         60 
MVPQALLFVP LLVFPLCFGK FPIYTILDKL GPWSPIDIHH LSCPNNLVVE DEGCTNLSGF 

        70         80         90        100        110        120 
SYMELKVGYI LAIKMNGFTC TGVVTEAETY TNFVGYVTTT FKRKHFRPTP DACRAAYNWK 

       130        140        150        160        170        180 
MAGDPRYEES LHNPYPDYRW LRTVKTTKES LVIISPSVAD LDPYDRSLHS RVFPSGKCSG 

       190        200        210        220        230        240 
VAVSSTYCST NHDYTIWMPE NPRLGMSCDI FTNSRGKRAS KGSETCGFVD ERGLYKSLKG 

       250        260        270        280        290        300 
ACKLKLCGVL GLRLMDGTWV AMQTSNETKW CPPDQLVNLH DFRSDEIEHL VVEELVRKRE 

       310        320        330        340        350        360 
ECLDALESIM TTKSVSFRRL SHLRKLVPGF GKAYTIFNKT LMEADAHYKS VRTWNEILPS 

       370        380        390        400        410        420 
KGCLRVGGRC HPHVNGVFFN GIILGPDGNV LIPEMQSSLL QQHMELLESS VIPLVHPLAD 

       430        440        450        460        470        480 
PSTVFKDGDE AEDFVEVHLP DVHNQVSGVD LGLPNWGKYV LLSAGALTAL MLIIFLMTCC 

       490        500        510        520 
RRVNRSEPTQ HNLRGTGREV SVTPQSGKII SSWESHKSGG ETRL 

« Hide

References

[1]"Structure of the glycoprotein gene in rabies virus."
Anilionis A., Wunner W.H., Curtis P.J.
Nature 294:275-278(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Amino acid sequence of the rabies virus glycoprotein deduced from its cloned gene."
Anilionis A., Wunner W.H., Curtis P.J.
Comp. Immunol. Microbiol. Infect. Dis. 5:27-32(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]"Complete nucleotide sequencing of SAD derivatives of attenuated rabies virus vaccine strains."
Geue L., Schares S., Schnick C., Kliemt J., Beckert A., Hoffmann B., Freuling C., Marston D., McElhinney L., Fooks A., Zanoni R., Peterhans E., Cox J.H., Mueller T.
Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: ERA 2007.
[4]"Antigenic variants of CVS rabies virus with altered glycosylation sites."
Wunner W.H., Dietzschold B., Smith C.L., Lafon M., Golub E.
Virology 140:1-12(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 49-59; 169-179; 209-226 AND 337-354.
Strain: CVS-11, RV194-2[F3] and RV231-22.
[5]"N-linked glycosylation of rabies virus glycoprotein. Individual sequons differ in their glycosylation efficiencies and influence on cell surface expression."
Shakin-Eshleman S.H., Remaley A.T., Eshleman J.R., Wunner W.H., Spitalnik S.L.
J. Biol. Chem. 267:10690-10698(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION AT ASN-56; ASN-266 AND ASN-338.
[6]"The role of site-specific N-glycosylation in secretion of soluble forms of rabies virus glycoprotein."
Wojczyk B.S., Stwora-Wojczyk M., Shakin-Eshleman S.H., Wunner W.H., Spitalnik S.L.
Glycobiology 8:121-130(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION.
[7]"Rabies virus receptors."
Lafon M.
J. Neurovirol. 11:82-87(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HOST CELL RECEPTORS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02293 Genomic RNA. Translation: AAA47204.1. Sequence problems.
M38452 Genomic RNA. Translation: AAA47209.1.
EF206707 Genomic RNA. Translation: ABN11294.1.
K02858 Genomic RNA. Translation: AAA47191.1.
K02859 Genomic RNA. Translation: AAA47192.1.
K02860 Genomic RNA. Translation: AAA47193.1.
K02861 Genomic RNA. Translation: AAA47194.1.
K02862 Genomic RNA. Translation: AAA47195.1.
K02863 Genomic RNA. Translation: AAA47196.1.
K02864 Genomic RNA. Translation: AAA47197.1.
K02865 Genomic RNA. Translation: AAA47198.1.
K02866 Genomic RNA. Translation: AAA47205.1.
K02867 Genomic RNA. Translation: AAA47206.1.
K02868 Genomic RNA. Translation: AAA47207.1.
K02869 Genomic RNA. Translation: AAA47208.1.
PIRVGVNG. A04121.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3NFKX-ray1.43C/D512-524[»]
ProteinModelPortalP03524.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001903. Rhabd_glycop.
[Graphical view]
PfamPF00974. Rhabdo_glycop. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVGLG_RABVE
AccessionPrimary (citable) accession number: P03524
Secondary accession number(s): A3F5L8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references