Reviewed, UniProtKB/Swiss-Prot P03523 (L_VSIVA)

Names and origin

Protein names

Recommended name:
    Large structural protein
      Short name=Protein L
Alternative name(s):
    Transcriptase
    Replicase
Including the following 3 domains:
    1- Recommended name:
            RNA-directed RNA polymerase
              EC=2.7.7.48
    2- Recommended name:
            mRNA (guanine-N(7)-)-methyltransferase
              EC=2.1.1.56
    3- Recommended name:
            mRNA guanylyltransferase
              EC=2.7.7.-

Gene names

Name:

L

Organism

Vesicular stomatitis Indiana virus (strain San Juan) (VSIV)

Taxonomic identifier

11285 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Dimarhabdovirus supergroup › Vesiculovirus

Virus host

Aedes [TaxID: 7158]
Simuliidae (black flies) [TaxID: 7190]
Musca domestica (House fly) [TaxID: 7370]
Homo sapiens (Human) [TaxID: 9606]
Equus asinus (Donkey) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]
Sus scrofa (Pig) [TaxID: 9823]
Bos taurus (Bovine) [TaxID: 9913]
Culicoides [TaxID: 58271]
Lutzomyia [TaxID: 252607]

Protein attributes

Sequence length	2109 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the rhabdoviridae protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW methylation Inferred from electronic annotation. Source: InterPro transcription, RNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2109	2109	Large structural protein		PRO_0000222844
Regions
	Domain	598 – 784	187	RdRp catalytic
	Nucleotide binding	1667 – 1676	10	ATP Potential
Natural variations
	Natural variant	228	1	P → R
	Natural variant	467	1	H → D
	Natural variant	548 – 549	2	FP → LR
	Natural variant	670	1	P → R
	Natural variant	910	1	S → F
	Natural variant	1026	1	P → A
	Natural variant	1348	1	A → G
	Natural variant	1589	1	P → A
	Natural variant	2026	1	I → T

Sequences

	Sequence		Length	Mass (Da)
	P03523-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 011EC2C0B5967C27	FASTA	2,109	240,883
	10 20 30 40 50 60 MEVHDFETDE FNDFNEDDYA TREFLNPDER MTYLNHADYN LNSPLISDDI DNLIRKFNSL 70 80 90 100 110 120 PIPSMWDSKN WDGVLEMLTS CQANPISTSQ MHKWMGSWLM SDNHDASQGY SFLHEVDKEA 130 140 150 160 170 180 EITFDVVETF IRGWGNKPIE YIKKERWTDS FKILAYLCQK FLDLHKLTLI LNAVSEVELL 190 200 210 220 230 240 NLARTFKGKV RRSSHGTNIC RIRVPSLGPT FISEGWAYFK KLDILMDPNF LLMVKDVIIG 250 260 270 280 290 300 RMQTVLSMVC RIDNLFSEQD IFSLLNIYRI GDKIVERQGN FSYDLIKMVE PICNLKLMKL 310 320 330 340 350 360 ARESRPLVPQ FPHFENHIKT SVDEGAKIDR GIRFLHDQIM SVKTVDLTLV IYGSFRHWGH 370 380 390 400 410 420 PFIDYYTGLE KLHSQVTMKK DIDVSYAKAL ASDLARIVLF QQFNDHKKWF VNGDLLPHDH 430 440 450 460 470 480 PFKSHVKENT WPTAAQVQDF GDKWHELPLI KCFEIPDLLD PSIIYSHKSH SMNRSEVLKH 490 500 510 520 530 540 VRMNPNTPIP SKKVLQTMLD TKATNWKEFL KEIDEKGLDD DDLIIGLKGK ERELKLAGRF 550 560 570 580 590 600 FSLMSWKFPE YFVITEYLIK THFVPMFKGL TMADDLTAVI KKMLDSSSGQ GLKSYEAICI 610 620 630 640 650 660 ANHIDYEKWN NHQRKLSNGP VFRVMGQFLG YPSLIERTHE FFEKSLIYYN GRPDLMRVHN 670 680 690 700 710 720 NTLINSTSQP VCWQGQEGGL EGLRQKGWTI LNLLVIQREA KIRNTAVKVL AQGDNQVICT 730 740 750 760 770 780 QYKTKKSRNV VELQGALNQM VSNNEKIMTA IKIGTGKLGL LINDDETMQS ADYLNYGKIP 790 800 810 820 830 840 IFRGVIRGLE TKRWSRVTCV TNDQIPTCAN IMSSVSTNAL TVAHFAENPI NAMIQYNYFG 850 860 870 880 890 900 TFARLLLMMH DPALRQSLYE VQDKIPGLHS STFKYAMLYL DPSIGGVSGM SLSRFLIRAF 910 920 930 940 950 960 PDPVTESLSS WRFIHVHARS EHLKEMSAVF GNPEIAKFRI THIDKLVEDP TSLNIAMGMS 970 980 990 1000 1010 1020 PANLLKTEVK KCLIESRQTI RNQVIKDATI YLYHEEDRLR SFLWSINPLF PRFLSEFKSG 1030 1040 1050 1060 1070 1080 TFLGVPDGLI SLFQNSRTIR NSFKKKYHRE LDDLIVRSEV SSLTHLGKLH LRRGSCKMWT 1090 1100 1110 1120 1130 1140 CSATHADTLR YKSWGRTVIG TTVPHPLEML GPQHRKETPC APCNTSGFNY VSVHCPDGIH 1150 1160 1170 1180 1190 1200 DVFSSRGPLP AYLGSKTSES TSILQPWERE SKVPLIKRAT RLRDAISWFV EPDSKLAMTI 1210 1220 1230 1240 1250 1260 LSNIHSLTGE EWTKRQHGFK RTGSALHRFS TSRMSHGGFA SQSTAALTRL MATTDTMRDL 1270 1280 1290 1300 1310 1320 GDQNFDFLFQ ATLLYAQITT TVARDGWITS CTDHYHIACK SCLRPIEEIT LDSSMDYTPP 1330 1340 1350 1360 1370 1380 DVSHVLKTWR NGEGSWGQEI KQIYPLEANW KNLAPAEQSY QVGRCIGFLY GDLAYRKSTH 1390 1400 1410 1420 1430 1440 AEDSSLFPLS IQGRIRGRGF LKGLLDGLMR ASCCQVIHRR SLAHLKRPAN AVYGGLIYLI 1450 1460 1470 1480 1490 1500 DKLSVSPPFL SLTRSGPIRD ELETIPHKIP TSYPTSNRDM GVIVRNYFKY QCRLIEKGKY 1510 1520 1530 1540 1550 1560 RSHYSQLWLF SDVLSIDFIG PFSISTTLLQ ILYKPFLSGK DKNELRELAN LSSLLRSGEG 1570 1580 1590 1600 1610 1620 WEDIHVKFFT KDILLCPEEI RHACKFGIPK DNNKDMSYPP WGRESRGTIT TIPVYYTTTP 1630 1640 1650 1660 1670 1680 YPKMLEMPPR IQNPLLSGIR LGQLPTGAHY KIRSILHGMG IHYRDFLSCG DGSGGMTAAL 1690 1700 1710 1720 1730 1740 LRENVHSRGI FNSLLELSGS VMRGASPEPP SALETLGGDK SRCVNGETCW EYPSDLCDPR 1750 1760 1770 1780 1790 1800 TWDYFLRLKA GLGLQIDLIV MDMEVRDSST SLKIETNVRN YVHRILDEQG VLIYKTYGTY 1810 1820 1830 1840 1850 1860 ICESEKNAVT ILGPMFKTVD LVQTEFSSSQ TSEVYMVCKG LKKLIDEPNP DWSSINESWK 1870 1880 1890 1900 1910 1920 NLYAFQSSEQ EFARAKKVST YFTLTGIPSQ FIPDPFVNIE TMLQIFGVPT GVSHAAALKS 1930 1940 1950 1960 1970 1980 SDRPADLLTI SLFYMAIISY YNINHIRVGP IPPNPPSDGI AQNVGIAITG ISFWLSLMEK 1990 2000 2010 2020 2030 2040 DIPLYQQCLA VIQQSFPIRW EAVSVKGGYK QKWSTRGDGL PKDTRISDSL APIGNWIRSL 2050 2060 2070 2080 2090 2100 ELVRNQVRLN PFNEILFNQL CRTVDNHLKW SNLRRNTGMI EWINRRISKE DRSILMLKSD LHEENSWRD

References

[1]	"Primary structure of the vesicular stomatitis virus polymerase (L) gene: evidence for a high frequency of mutations." Schubert M., Harmison G.G., Meier E. J. Virol. 51:505-514(1984) [PubMed: 6086959] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X00939 Genomic RNA. Translation: CAA25453.1. J02428 Genomic RNA. Translation: AAA48371.1.
PIR	ZLVN. A04119.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. IPR017234. RNA-dir_pol_rhabdovirus. IPR001016. RNA_pol_L_viral. IPR002877. rRNA_MeTrfase_RrmJ/FtsJ. [Graphical view]
Pfam	PF01728. FtsJ. 1 hit. PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF037546. RNA_pol_RhabdoV_sub. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_VSIVA

Accession

Primary (citable) accession number: P03523
Secondary accession number(s): Q86125

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	November 1, 1995
Last modified:	January 19, 2010
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)