Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycoprotein

Gene

G

Organism
Vesicular stomatitis Indiana virus (strain San Juan) (VSIV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Attaches the virus to host LDL receptors, inducing clathrin-dependent endocytosis of the virion.2 Publications
In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and endosomal membrane.2 Publications

GO - Biological processi

Keywordsi

Biological processClathrin-mediated endocytosis of virus by host, Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Glycoprotein
Gene namesi
Name:G
OrganismiVesicular stomatitis Indiana virus (strain San Juan) (VSIV)
Taxonomic identifieri11285 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesRhabdoviridaeVesiculovirus
Virus hostiAedes [TaxID: 7158]
Bos taurus (Bovine) [TaxID: 9913]
Culicoides [TaxID: 58271]
Equus asinus (Donkey) (Equus africanus asinus) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]
Homo sapiens (Human) [TaxID: 9606]
Lutzomyia [TaxID: 252607]
Musca domestica (House fly) [TaxID: 7370]
Simuliidae (black flies) [TaxID: 7190]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000002327 Componenti: Genome

Subcellular locationi

  • Virion membrane 2 Publications; Single-pass type I membrane protein 1 Publication
  • Host membrane 1 Publication; Single-pass type I membrane protein 1 Publication
  • Note: The cytoplasmic domain sorts the protein to neurons dentrites instead of axons. When expressed in ex vivo polarized cells like epithelial cells, it sorts the protein to the basolateral side.

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini17 – 467Virion surfaceSequence analysisAdd BLAST451
Transmembranei468 – 488HelicalSequence analysisAdd BLAST21
Topological domaini489 – 511IntravirionSequence analysisAdd BLAST23

GO - Cellular componenti

Keywords - Cellular componenti

Host membrane, Membrane, Viral envelope protein, Virion

Pathology & Biotechi

Biotechnological usei

Used to pseudotype many virus-like particles like lentiviral vector, because of its broad spectrum of host cell tropism. Also used in viral vectors studies in cancer therapy.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi489C → S: Complete loss of palmitoylation. 1 Publication1
Mutagenesisi496K → A: No effect on basolateral targeting ex vivo. 1 Publication1
Mutagenesisi497K → A: No effect on basolateral targeting ex vivo. 1 Publication1
Mutagenesisi498R → A: No effect on basolateral targeting ex vivo. 1 Publication1
Mutagenesisi499Q → A: No effect on basolateral targeting ex vivo. 1 Publication1
Mutagenesisi500I → A: No effect on basolateral targeting ex vivo. 1 Publication1
Mutagenesisi501Y → A: Complete loss of basolateral targeting ex vivo. 1 Publication1
Mutagenesisi502T → A: No effect on basolateral targeting ex vivo. 1 Publication1
Mutagenesisi503D → A: No effect on basolateral targeting ex vivo. 1 Publication1
Mutagenesisi504I → A: Complete loss of basolateral targeting ex vivo. 1 Publication1
Mutagenesisi505E → A: No effect on basolateral targeting ex vivo. 1 Publication1
Mutagenesisi506M → A: No effect on basolateral targeting ex vivo. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Add BLAST16
ChainiPRO_000004100517 – 511GlycoproteinAdd BLAST495

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi40 ↔ 300By similarity
Disulfide bondi75 ↔ 108By similarity
Disulfide bondi84 ↔ 130By similarity
Disulfide bondi169 ↔ 174By similarity
Glycosylationi179N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Disulfide bondi193 ↔ 240By similarity
Disulfide bondi235 ↔ 269By similarity
Glycosylationi336N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Lipidationi489S-palmitoyl cysteine; by host1 Publication1

Post-translational modificationi

Glycosylated by host.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Interactioni

Subunit structurei

Homotrimer. Interacts with host LDL at target cell surface (PubMed:23589850).1 Publication

Protein-protein interaction databases

ELMiP03522.

Structurei

Secondary structure

1511
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni504 – 506Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EGDX-ray2.70D499-508[»]
ProteinModelPortaliP03522.
SMRiP03522.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03522.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi496 – 506basolateral targeting ex vivoAdd BLAST11

Sequence similaritiesi

Belongs to the vesiculovirus glycoprotein family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG09000070.

Family and domain databases

InterProiView protein in InterPro
IPR001903. Rhabd_glycop.
PfamiView protein in Pfam
PF00974. Rhabdo_glycop. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03522-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKCLLYLAFL FIGVNCKFTI VFPHNQKGNW KNVPSNYHYC PSSSDLNWHN
60 70 80 90 100
DLIGTAIQVK MPKSHKAIQA DGWMCHASKW VTTCDFRWYG PKYITQSIRS
110 120 130 140 150
FTPSVEQCKE SIEQTKQGTW LNPGFPPQSC GYATVTDAEA VIVQVTPHHV
160 170 180 190 200
LVDEYTGEWV DSQFINGKCS NYICPTVHNS TTWHSDYKVK GLCDSNLISM
210 220 230 240 250
DITFFSEDGE LSSLGKEGTG FRSNYFAYET GGKACKMQYC KHWGVRLPSG
260 270 280 290 300
VWFEMADKDL FAAARFPECP EGSSISAPSQ TSVDVSLIQD VERILDYSLC
310 320 330 340 350
QETWSKIRAG LPISPVDLSY LAPKNPGTGP AFTIINGTLK YFETRYIRVD
360 370 380 390 400
IAAPILSRMV GMISGTTTER ELWDDWAPYE DVEIGPNGVL RTSSGYKFPL
410 420 430 440 450
YMIGHGMLDS DLHLSSKAQV FEHPHIQDAA SQLPDDESLF FGDTGLSKNP
460 470 480 490 500
IELVEGWFSS WKSSIASFFF IIGLIIGLFL VLRVGIHLCI KLKHTKKRQI
510
YTDIEMNRLG K
Length:511
Mass (Da):57,482
Last modified:July 21, 1986 - v1
Checksum:i26E94F713AC0B8E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02428 Genomic RNA. Translation: AAA48370.1.
M35219 Genomic RNA. Translation: AAA48389.1.
RefSeqiNP_041715.1. NC_001560.1.

Genome annotation databases

GeneIDi1489834.
KEGGivg:1489834.

Similar proteinsi

Entry informationi

Entry nameiGLYCO_VSIVA
AccessioniPrimary (citable) accession number: P03522
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: August 30, 2017
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families