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Protein

Nucleoprotein

Gene

N

Organism
Vesicular stomatitis Indiana virus (strain San Juan) (VSIV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Nucleocapsid assembly is concommitant with replication, therefore viral replication dependscc on the intracellular concentration of free N, termed N0. All replicative products are resistant to nucleases.2 Publications

GO - Molecular functioni

  • RNA binding Source: UniProtKB

GO - Biological processi

  • RNA replication Source: UniProtKB
  • viral transcription Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Short name:
NP
Alternative name(s):
Nucleocapsid protein
Short name:
Protein N
Gene namesi
Name:N
OrganismiVesicular stomatitis Indiana virus (strain San Juan) (VSIV)
Taxonomic identifieri11285 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesRhabdoviridaeVesiculovirus
Virus hostiAedes [TaxID: 7158]
Bos taurus (Bovine) [TaxID: 9913]
Culicoides [TaxID: 58271]
Equus asinus (Donkey) (Equus africanus asinus) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]
Homo sapiens (Human) [TaxID: 9606]
Lutzomyia [TaxID: 252607]
Musca domestica (House fly) [TaxID: 7370]
Simuliidae (black flies) [TaxID: 7190]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000002327 Componenti: Genome

Subcellular locationi

  • Virion 1 Publication
  • Host cytoplasm

  • Note: The nucleocapsid is synthesized in the cytoplasm, and is subsequently transported via microtubules to the cell periphery.

GO - Cellular componenti

  • helical viral capsid Source: UniProtKB-KW
  • host cell cytoplasm Source: UniProtKB-SubCell
  • viral nucleocapsid Source: UniProtKB
  • virion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002228231 – 422NucleoproteinAdd BLAST422

Interactioni

Subunit structurei

Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA (PubMed:11000221). N in nucleocapsid binds the P protein and thereby positions the polymerase on the template (PubMed:3016338). Interaction of N0 with the P protein prevents the uncontrolled aggregation of N0 (PubMed:21207454).3 Publications

Structurei

Secondary structure

1422
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 7Combined sources3
Turni8 – 11Combined sources4
Beta strandi12 – 14Combined sources3
Helixi29 – 31Combined sources3
Turni32 – 34Combined sources3
Helixi48 – 59Combined sources12
Turni60 – 62Combined sources3
Helixi66 – 76Combined sources11
Beta strandi88 – 90Combined sources3
Beta strandi93 – 96Combined sources4
Turni130 – 133Combined sources4
Helixi134 – 144Combined sources11
Helixi151 – 163Combined sources13
Turni164 – 167Combined sources4
Beta strandi178 – 182Combined sources5
Helixi185 – 187Combined sources3
Helixi189 – 204Combined sources16
Turni205 – 207Combined sources3
Helixi211 – 213Combined sources3
Helixi214 – 217Combined sources4
Helixi218 – 220Combined sources3
Turni221 – 224Combined sources4
Helixi226 – 238Combined sources13
Helixi242 – 247Combined sources6
Helixi252 – 261Combined sources10
Beta strandi264 – 266Combined sources3
Beta strandi270 – 272Combined sources3
Helixi275 – 277Combined sources3
Turni278 – 282Combined sources5
Turni291 – 293Combined sources3
Helixi295 – 307Combined sources13
Helixi313 – 315Combined sources3
Beta strandi320 – 322Combined sources3
Helixi324 – 340Combined sources17
Turni365 – 367Combined sources3
Helixi375 – 383Combined sources9
Turni384 – 387Combined sources4
Helixi391 – 401Combined sources11
Helixi412 – 420Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FZJX-ray1.90P52-59[»]
1FZMX-ray1.80P52-59[»]
2GICX-ray2.92A/B/C/D/E1-422[»]
2QVJX-ray2.80A/B/C/D/E2-422[»]
2WYYelectron microscopy10.00A/C/D/F/H/I/J/K/L/M1-422[»]
3PTOX-ray3.01A/B/C/D/E2-422[»]
3PTXX-ray3.00A/B/C/D/E2-422[»]
3PU0X-ray3.09A/B/C/D/E2-422[»]
3PU1X-ray3.14A/B/C/D/E2-422[»]
3PU4X-ray3.00A/B/C/D/E2-422[»]
ProteinModelPortaliP03521.
SMRiP03521.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03521.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.3570.10. 1 hit.
1.10.3610.10. 1 hit.
InterProiIPR000448. Rhabdo_ncapsid.
IPR023331. Rhabdovirus_ncapsid_C.
IPR023330. Rhabdovirus_ncapsid_N.
[Graphical view]
PfamiPF00945. Rhabdo_ncap. 1 hit.
[Graphical view]
ProDomiPD002087. Rhabd_nucleocap. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF140809. SSF140809. 1 hit.

Sequencei

Sequence statusi: Complete.

P03521-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVTVKRIID NTVIVPKLPA NEDPVEYPAD YFRKSKEIPL YINTTKSLSD
60 70 80 90 100
LRGYVYQGLK SGNVSIIHVN SYLYGALKDI RGKLDKDWSS FGINIGKAGD
110 120 130 140 150
TIGIFDLVSL KALDGVLPDG VSDASRTSAD DKWLPLYLLG LYRVGRTQMP
160 170 180 190 200
EYRKKLMDGL TNQCKMINEQ FEPLVPEGRD IFDVWGNDSN YTKIVAAVDM
210 220 230 240 250
FFHMFKKHEC ASFRYGTIVS RFKDCAALAT FGHLCKITGM STEDVTTWIL
260 270 280 290 300
NREVADEMVQ MMLPGQEIDK ADSYMPYLID FGLSSKSPYS SVKNPAFHFW
310 320 330 340 350
GQLTALLLRS TRARNARQPD DIEYTSLTTA GLLYAYAVGS SADLAQQFCV
360 370 380 390 400
GDNKYTPDDS TGGLTTNAPP QGRDVVEWLG WFEDQNRKPT PDMMQYAKRA
410 420
VMSLQGLREK TIGKYAKSEF DK
Length:422
Mass (Da):47,409
Last modified:July 21, 1986 - v1
Checksum:i7345C01BAB3C710E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02428 Genomic RNA. Translation: AAA48367.1.
RefSeqiNP_041712.1. NC_001560.1.

Genome annotation databases

GeneIDi1489831.
KEGGivg:1489831.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02428 Genomic RNA. Translation: AAA48367.1.
RefSeqiNP_041712.1. NC_001560.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FZJX-ray1.90P52-59[»]
1FZMX-ray1.80P52-59[»]
2GICX-ray2.92A/B/C/D/E1-422[»]
2QVJX-ray2.80A/B/C/D/E2-422[»]
2WYYelectron microscopy10.00A/C/D/F/H/I/J/K/L/M1-422[»]
3PTOX-ray3.01A/B/C/D/E2-422[»]
3PTXX-ray3.00A/B/C/D/E2-422[»]
3PU0X-ray3.09A/B/C/D/E2-422[»]
3PU1X-ray3.14A/B/C/D/E2-422[»]
3PU4X-ray3.00A/B/C/D/E2-422[»]
ProteinModelPortaliP03521.
SMRiP03521.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1489831.
KEGGivg:1489831.

Miscellaneous databases

EvolutionaryTraceiP03521.

Family and domain databases

Gene3Di1.10.3570.10. 1 hit.
1.10.3610.10. 1 hit.
InterProiIPR000448. Rhabdo_ncapsid.
IPR023331. Rhabdovirus_ncapsid_C.
IPR023330. Rhabdovirus_ncapsid_N.
[Graphical view]
PfamiPF00945. Rhabdo_ncap. 1 hit.
[Graphical view]
ProDomiPD002087. Rhabd_nucleocap. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF140809. SSF140809. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNCAP_VSIVA
AccessioniPrimary (citable) accession number: P03521
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 30, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.