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Protein

Nucleoprotein

Gene

N

Organism
Vesicular stomatitis Indiana virus (strain San Juan) (VSIV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Nucleocapsid assembly is concommitant with replication, therefore viral replication dependscc on the intracellular concentration of free N, termed N0. All replicative products are resistant to nucleases.2 Publications

GO - Molecular functioni

  • RNA binding Source: UniProtKB

GO - Biological processi

  • RNA replication Source: UniProtKB
  • viral transcription Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

Viral RNA replication, Viral transcription

Keywords - Ligandi

RNA-binding, Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoprotein
Short name:
NP
Alternative name(s):
Nucleocapsid protein
Short name:
Protein N
Gene namesi
Name:N
OrganismiVesicular stomatitis Indiana virus (strain San Juan) (VSIV)
Taxonomic identifieri11285 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesRhabdoviridaeVesiculovirus
Virus hostiAedes [TaxID: 7158]
Bos taurus (Bovine) [TaxID: 9913]
Culicoides [TaxID: 58271]
Equus asinus (Donkey) (Equus africanus asinus) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]
Homo sapiens (Human) [TaxID: 9606]
Lutzomyia [TaxID: 252607]
Musca domestica (House fly) [TaxID: 7370]
Simuliidae (black flies) [TaxID: 7190]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000002327 Componenti: Genome

Subcellular locationi

  • Virion 1 Publication
  • Host cytoplasm

  • Note: The nucleocapsid is synthesized in the cytoplasm, and is subsequently transported via microtubules to the cell periphery.

GO - Cellular componenti

  • helical viral capsid Source: UniProtKB-KW
  • host cell cytoplasm Source: UniProtKB-SubCell
  • viral nucleocapsid Source: UniProtKB
  • virion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Helical capsid protein, Host cytoplasm, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 422422NucleoproteinPRO_0000222823Add
BLAST

Interactioni

Subunit structurei

Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA (PubMed:11000221). N in nucleocapsid binds the P protein and thereby positions the polymerase on the template (PubMed:3016338). Interaction of N0 with the P protein prevents the uncontrolled aggregation of N0 (PubMed:21207454).3 Publications

Structurei

Secondary structure

1
422
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 73Combined sources
Turni8 – 114Combined sources
Beta strandi12 – 143Combined sources
Helixi29 – 313Combined sources
Turni32 – 343Combined sources
Helixi48 – 5912Combined sources
Turni60 – 623Combined sources
Helixi66 – 7611Combined sources
Beta strandi88 – 903Combined sources
Beta strandi93 – 964Combined sources
Turni130 – 1334Combined sources
Helixi134 – 14411Combined sources
Helixi151 – 16313Combined sources
Turni164 – 1674Combined sources
Beta strandi178 – 1825Combined sources
Helixi185 – 1873Combined sources
Helixi189 – 20416Combined sources
Turni205 – 2073Combined sources
Helixi211 – 2133Combined sources
Helixi214 – 2174Combined sources
Helixi218 – 2203Combined sources
Turni221 – 2244Combined sources
Helixi226 – 23813Combined sources
Helixi242 – 2476Combined sources
Helixi252 – 26110Combined sources
Beta strandi264 – 2663Combined sources
Beta strandi270 – 2723Combined sources
Helixi275 – 2773Combined sources
Turni278 – 2825Combined sources
Turni291 – 2933Combined sources
Helixi295 – 30713Combined sources
Helixi313 – 3153Combined sources
Beta strandi320 – 3223Combined sources
Helixi324 – 34017Combined sources
Turni365 – 3673Combined sources
Helixi375 – 3839Combined sources
Turni384 – 3874Combined sources
Helixi391 – 40111Combined sources
Helixi412 – 4209Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FZJX-ray1.90P52-59[»]
1FZMX-ray1.80P52-59[»]
2GICX-ray2.92A/B/C/D/E1-422[»]
2QVJX-ray2.80A/B/C/D/E2-422[»]
2WYYelectron microscopy10.00A/C/D/F/H/I/J/K/L/M1-422[»]
3PTOX-ray3.01A/B/C/D/E2-422[»]
3PTXX-ray3.00A/B/C/D/E2-422[»]
3PU0X-ray3.09A/B/C/D/E2-422[»]
3PU1X-ray3.14A/B/C/D/E2-422[»]
3PU4X-ray3.00A/B/C/D/E2-422[»]
ProteinModelPortaliP03521.
SMRiP03521. Positions 2-422.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03521.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.3570.10. 1 hit.
1.10.3610.10. 1 hit.
InterProiIPR000448. Rhabdo_ncapsid.
IPR023331. Rhabdovirus_ncapsid_C.
IPR023330. Rhabdovirus_ncapsid_N.
[Graphical view]
PfamiPF00945. Rhabdo_ncap. 1 hit.
[Graphical view]
ProDomiPD002087. Rhabd_nucleocap. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF140809. SSF140809. 1 hit.

Sequencei

Sequence statusi: Complete.

P03521-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVTVKRIID NTVIVPKLPA NEDPVEYPAD YFRKSKEIPL YINTTKSLSD
60 70 80 90 100
LRGYVYQGLK SGNVSIIHVN SYLYGALKDI RGKLDKDWSS FGINIGKAGD
110 120 130 140 150
TIGIFDLVSL KALDGVLPDG VSDASRTSAD DKWLPLYLLG LYRVGRTQMP
160 170 180 190 200
EYRKKLMDGL TNQCKMINEQ FEPLVPEGRD IFDVWGNDSN YTKIVAAVDM
210 220 230 240 250
FFHMFKKHEC ASFRYGTIVS RFKDCAALAT FGHLCKITGM STEDVTTWIL
260 270 280 290 300
NREVADEMVQ MMLPGQEIDK ADSYMPYLID FGLSSKSPYS SVKNPAFHFW
310 320 330 340 350
GQLTALLLRS TRARNARQPD DIEYTSLTTA GLLYAYAVGS SADLAQQFCV
360 370 380 390 400
GDNKYTPDDS TGGLTTNAPP QGRDVVEWLG WFEDQNRKPT PDMMQYAKRA
410 420
VMSLQGLREK TIGKYAKSEF DK
Length:422
Mass (Da):47,409
Last modified:July 21, 1986 - v1
Checksum:i7345C01BAB3C710E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02428 Genomic RNA. Translation: AAA48367.1.
RefSeqiNP_041712.1. NC_001560.1.

Genome annotation databases

GeneIDi1489831.
KEGGivg:1489831.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02428 Genomic RNA. Translation: AAA48367.1.
RefSeqiNP_041712.1. NC_001560.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FZJX-ray1.90P52-59[»]
1FZMX-ray1.80P52-59[»]
2GICX-ray2.92A/B/C/D/E1-422[»]
2QVJX-ray2.80A/B/C/D/E2-422[»]
2WYYelectron microscopy10.00A/C/D/F/H/I/J/K/L/M1-422[»]
3PTOX-ray3.01A/B/C/D/E2-422[»]
3PTXX-ray3.00A/B/C/D/E2-422[»]
3PU0X-ray3.09A/B/C/D/E2-422[»]
3PU1X-ray3.14A/B/C/D/E2-422[»]
3PU4X-ray3.00A/B/C/D/E2-422[»]
ProteinModelPortaliP03521.
SMRiP03521. Positions 2-422.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1489831.
KEGGivg:1489831.

Miscellaneous databases

EvolutionaryTraceiP03521.

Family and domain databases

Gene3Di1.10.3570.10. 1 hit.
1.10.3610.10. 1 hit.
InterProiIPR000448. Rhabdo_ncapsid.
IPR023331. Rhabdovirus_ncapsid_C.
IPR023330. Rhabdovirus_ncapsid_N.
[Graphical view]
PfamiPF00945. Rhabdo_ncap. 1 hit.
[Graphical view]
ProDomiPD002087. Rhabd_nucleocap. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF140809. SSF140809. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNCAP_VSIVA
AccessioniPrimary (citable) accession number: P03521
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 8, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.