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Protein

Matrix protein

Gene

M

Organism
Vesicular stomatitis Indiana virus (strain San Juan) (VSIV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a major role in assembly and budding of virion, by recruiting cellular partners of the ESCRT complexes that play a key role in releasing the budding particle from the host membrane. Condensates the ribonucleocapsid core during virus assembly.2 Publications
Shut off cellular protein expression by inhibiting mRNA nuclear export through direct interaction with host RAE1-NUP98 complex. This shutoff presumably inhibits interferon signaling and thus establishment of antiviral state in virus infected cells. Induces cell-rounding, cytoskeleton disorganization and apoptosis in infected cell.1 Publication

Miscellaneous

Most abundant protein in the virion.

GO - Molecular functioni

GO - Biological processi

  • phosphorylation Source: UniProtKB
  • suppression by virus of host gene expression Source: UniProtKB-KW
  • suppression by virus of host mRNA export from nucleus Source: UniProtKB
  • viral budding via host ESCRT complex Source: UniProtKB
  • viral release from host cell Source: UniProtKB-KW

Keywordsi

Biological processApoptosis, Eukaryotic host gene expression shutoff by virus, Host gene expression shutoff by virus, Host mRNA suppression by virus, Host-virus interaction, Inhibition of host mRNA nuclear export by virus, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Matrix protein
Gene namesi
Name:M
OrganismiVesicular stomatitis Indiana virus (strain San Juan) (VSIV)
Taxonomic identifieri11285 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesMononegaviralesRhabdoviridaeVesiculovirus
Virus hostiAedes [TaxID: 7158]
Bos taurus (Bovine) [TaxID: 9913]
Culicoides [TaxID: 58271]
Equus asinus (Donkey) (Equus africanus asinus) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]
Homo sapiens (Human) [TaxID: 9606]
Lutzomyia [TaxID: 252607]
Musca domestica (House fly) [TaxID: 7370]
Simuliidae (black flies) [TaxID: 7190]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000002327 Componenti: Genome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Host membrane, Host nucleus, Membrane, Viral matrix protein, Virion

Pathology & Biotechi

Biotechnological usei

VSV is used as an oncolytic agent for cancer therapy, because of his wide host range, rapid replication and mild pathogenicity in humans. VSV used are mutated at M51R in their matrix protein. These mutated viruses cannot successfully infect normal cells, being unable to counteract the antiviral state induced by interferon-alpha in normal cells. Cancer cells are impeded with responsiveness to interferon, and then can be successfully infected and lysed by the virus.

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi4L → A: No effect on host NEDD4 or TSG101 binding. 1 Publication1
Mutagenesisi5 – 7KKI → AAA: No effect on mRNA nuclear export inhibition. 1 Publication3
Mutagenesisi27Y → A: Partial loss of host NEDD4 binding. 1 Publication1
Mutagenesisi28 – 30EED → AAA: No effect on mRNA nuclear export inhibition. 1 Publication3
Mutagenesisi40P → A: Partial loss of host TSG101 binding. 1 Publication1
Mutagenesisi42 – 44DKS → AAA: No effect on mRNA nuclear export inhibition. 1 Publication3
Mutagenesisi51M → R: Complete loss of mRNA nuclear export inhibition. 1 Publication1
Mutagenesisi52 – 54DTY → AAA: Complete loss of mRNA nuclear export inhibition. 1 Publication3
Mutagenesisi61 – 63YEK → AAA: No effect on mRNA nuclear export inhibition. 1 Publication3
Mutagenesisi121 – 124AVLA → DKQQ: No effect on virion budding. Increase viral-mRNA translation. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002228571 – 229Matrix proteinAdd BLAST229

Post-translational modificationi

Phosphorylated by host.1 Publication

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homomultimer. Interacts with viral nucleocapsid. Interacts with host NEDD4 and TSG101 (PubMed:22190013). Interacts with host dynamin (PubMed:20943988). Interacts with host RAE1-NUP98 complex (PubMed:11106761, PubMed:15629720).4 Publications

Protein-protein interaction databases

IntActiP03519. 3 interactors.
MINTiMINT-7294425.

Structurei

3D structure databases

ProteinModelPortaliP03519.
SMRiP03519.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi2 – 4dynamin binding1 Publication3
Motifi24 – 27PPXY motif4
Motifi37 – 40PTAP/PSAP motif4

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. M contains two overlapping L domains: a PPXY motif which interacts with the WW domain 3 of NEDD4 and a PTAP/PSAP motif, which interacts with the UEV domain of TSG101.2 Publications

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG090000OM.

Family and domain databases

Gene3Di3.10.460.10. 1 hit.
InterProiView protein in InterPro
IPR009397. Vesiculo_matrix.
PfamiView protein in Pfam
PF06326. Vesiculo_matrix. 1 hit.
SUPFAMiSSF75404. SSF75404. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform M (identifier: P03519-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSLKKILGL KGKGKKSKKL GIAPPPYEED TSMEYAPSAP IDKSYFGVDE
60 70 80 90 100
MDTYDPNQLR YEKFFFTVKM TVRSNRPFRT YSDVAAAVSH WDHMYIGMAG
110 120 130 140 150
KRPFYKILAF LGSSNLKATP AVLADQGQPE YHTHCEGRAY LPHRMGKTPP
160 170 180 190 200
MLNVPEHFRR PFNIGLYKGT IELTMTIYDD ESLEAAPMIW DHFNSSKFSD
210 220
FREKALMFGL IVEKKASGAW VLDSISHFK
Length:229
Mass (Da):26,094
Last modified:July 21, 1986 - v1
Checksum:iC89E2ACA4365B847
GO
Isoform M21 Publication (identifier: P03519-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.

Show »
Length:197
Mass (Da):22,682
Checksum:i57DB605CB2F2DDF2
GO
Isoform M31 Publication (identifier: P03519-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-50: Missing.

Show »
Length:179
Mass (Da):20,681
Checksum:iEABE4EA02CA422E6
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0254201 – 50Missing in isoform M3. CuratedAdd BLAST50
Alternative sequenceiVSP_0254211 – 32Missing in isoform M2. CuratedAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02428 Genomic RNA. Translation: AAA48369.1.
RefSeqiNP_041714.1. NC_001560.1.

Genome annotation databases

GeneIDi1489833.
KEGGivg:1489833.

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMATRX_VSIVA
AccessioniPrimary (citable) accession number: P03519
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: March 15, 2017
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families