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Protein

Envelopment polyprotein

Gene

GP

Organism
Rift valley fever virus (RVFV)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glycoprotein N: interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity).By similarity
Glycoprotein C: interact with each other and are present at the surface of the virion. Together they play a role in attachment of the virion to a cell receptor. Promotes fusion of membranes after endocytosis of the virion (By similarity).By similarity
NSm protein: Plays a role for virus dissemination in mouse.By similarity
NSm-Gn protein: Plays a role for virus dissemination in mosquitoes.By similarity

GO - Biological processi

Keywordsi

Biological processFusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelopment polyprotein
Alternative name(s):
M polyprotein
Cleaved into the following 3 chains:
NSm-Gn proteinBy similarity
Glycoprotein NBy similarity
Short name:
Gn
Alternative name(s):
Glycoprotein G1
Glycoprotein CBy similarity
Short name:
Gc
Alternative name(s):
Glycoprotein G2
Gene namesi
Name:GP
OrganismiRift valley fever virus (RVFV)
Taxonomic identifieri11588 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesBunyaviralesPhenuiviridaePhlebovirus
Virus hostiAedes [TaxID: 7158]
Bos taurus (Bovine) [TaxID: 9913]
Bos taurus x Bison bison (beefalo) [TaxID: 297284]
Camelus bactrianus (Bactrian camel) [TaxID: 9837]
Capra hircus (Goat) [TaxID: 9925]
Homo sapiens (Human) [TaxID: 9606]
Ovis aries (Sheep) [TaxID: 9940]
Phlebotomus papatasi (Sandfly) [TaxID: 29031]

Subcellular locationi

Glycoprotein N :
  • Virion membrane By similarity; Single-pass type I membrane protein By similarity
  • Host Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity
  • Host endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity
  • Note: Interaction between G1 and G2 is essential for proper targeting of G1 to the Golgi complex, where virion budding occurs.By similarity
Glycoprotein C :
  • Virion membrane By similarity; Single-pass type I membrane protein By similarity
  • Host Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini17 – 582LumenalSequence analysisAdd BLAST566
Transmembranei583 – 603HelicalSequence analysisAdd BLAST21
Topological domaini604 – 690CytoplasmicSequence analysisAdd BLAST87
Topological domaini691 – 1136LumenalSequence analysisAdd BLAST446
Transmembranei1137 – 1157HelicalSequence analysisAdd BLAST21
Topological domaini1158 – 1206CytoplasmicSequence analysisAdd BLAST49

GO - Cellular componenti

Keywords - Cellular componenti

Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
ChainiPRO_000024700917 – 1206Envelopment polyproteinAdd BLAST1190
ChainiPRO_000003684717 – 153NSm-Gn proteinAdd BLAST137
ChainiPRO_0000036848154 – 690Glycoprotein NSequence analysisAdd BLAST537
ChainiPRO_0000036849691 – 1206Glycoprotein CSequence analysisAdd BLAST516

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi88N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi438N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi794N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi1035N-linked (GlcNAc...) asparagine; by hostSequence analysis1
Glycosylationi1077N-linked (GlcNAc...) asparagine; by hostSequence analysis1

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins including NSm protein, Glycoprotein C, and Glycoprotein N.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei690 – 691Cleavage; by host signal peptidaseBy similarity2

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP03518

Interactioni

Subunit structurei

Glycoprotein C and Glycoprotein N interacts with each other.By similarity

Structurei

Secondary structure

11206
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni156 – 159Combined sources4
Beta strandi162 – 164Combined sources3
Helixi176 – 179Combined sources4
Beta strandi184 – 186Combined sources3
Turni189 – 191Combined sources3
Helixi192 – 195Combined sources4
Helixi201 – 205Combined sources5
Turni206 – 208Combined sources3
Helixi212 – 217Combined sources6
Beta strandi225 – 227Combined sources3
Helixi237 – 244Combined sources8
Beta strandi255 – 260Combined sources6
Beta strandi264 – 270Combined sources7
Beta strandi275 – 277Combined sources3
Beta strandi284 – 286Combined sources3
Beta strandi288 – 290Combined sources3
Beta strandi294 – 298Combined sources5
Beta strandi300 – 304Combined sources5
Beta strandi321 – 324Combined sources4
Beta strandi336 – 347Combined sources12
Beta strandi353 – 355Combined sources3
Beta strandi361 – 364Combined sources4
Helixi369 – 371Combined sources3
Beta strandi372 – 377Combined sources6
Beta strandi394 – 397Combined sources4
Helixi399 – 401Combined sources3
Beta strandi413 – 415Combined sources3
Helixi417 – 422Combined sources6
Turni427 – 429Combined sources3
Beta strandi431 – 436Combined sources6
Beta strandi441 – 447Combined sources7
Beta strandi450 – 452Combined sources3
Beta strandi455 – 467Combined sources13
Beta strandi691 – 696Combined sources6
Helixi699 – 701Combined sources3
Beta strandi702 – 709Combined sources8
Beta strandi711 – 722Combined sources12
Beta strandi728 – 735Combined sources8
Beta strandi742 – 764Combined sources23
Beta strandi767 – 776Combined sources10
Turni785 – 790Combined sources6
Helixi798 – 803Combined sources6
Beta strandi810 – 817Combined sources8
Helixi821 – 823Combined sources3
Beta strandi826 – 829Combined sources4
Beta strandi831 – 844Combined sources14
Beta strandi847 – 864Combined sources18
Beta strandi870 – 875Combined sources6
Beta strandi880 – 883Combined sources4
Beta strandi886 – 893Combined sources8
Helixi900 – 902Combined sources3
Beta strandi904 – 908Combined sources5
Turni909 – 911Combined sources3
Beta strandi912 – 918Combined sources7
Beta strandi931 – 936Combined sources6
Helixi937 – 941Combined sources5
Beta strandi947 – 949Combined sources3
Beta strandi954 – 959Combined sources6
Beta strandi962 – 967Combined sources6
Helixi972 – 978Combined sources7
Beta strandi980 – 985Combined sources6
Beta strandi988 – 992Combined sources5
Beta strandi994 – 996Combined sources3
Beta strandi999 – 1003Combined sources5
Beta strandi1009 – 1021Combined sources13
Beta strandi1030 – 1044Combined sources15
Beta strandi1046 – 1056Combined sources11
Beta strandi1058 – 1064Combined sources7
Beta strandi1070 – 1075Combined sources6
Beta strandi1077 – 1086Combined sources10
Beta strandi1089 – 1103Combined sources15
Beta strandi1106 – 1113Combined sources8
Beta strandi1123 – 1129Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HJ1X-ray1.90A/B/C/D691-1119[»]
4HJCX-ray4.15A691-1118[»]
5Y0WX-ray2.50A154-469[»]
5Y0YX-ray3.40A154-469[»]
6EGTX-ray2.50A/B/C691-1158[»]
6EGUX-ray2.30A/B/C691-1158[»]
SMRiP03518
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiView protein in InterPro
IPR016404 M_polyprot_prcur_phlebovir
IPR010826 Phlebovirus_G1
IPR009878 Phlebovirus_G2
IPR009879 Phlebovirus_NSM
PfamiView protein in Pfam
PF07243 Phlebovirus_G1, 1 hit
PF07245 Phlebovirus_G2, 1 hit
PF07246 Phlebovirus_NSM, 2 hits
PIRSFiPIRSF003961 M_poly_PhleboV, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: P03518-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYVLLTILIS VLVCEAVIRV SLSSTREETC FGDSTNPEMI EGAWDSLREE
60 70 80 90 100
EMPEELSCSI SGIREVKTSS QELYRALKAI IAADGLNNIT CHGKDPEDKI
110 120 130 140 150
SLIKGPPHKK RVGIVRCERR RDAKQIGRET MAGIAMTVLP ALAVFALAPV
160 170 180 190 200
VFAEDPHLRN RPGKGHNYID GMTQEDATCK PVTYAGACSS FDVLLEKGKF
210 220 230 240 250
PLFQSYAHHR TLLEAVHDTI IAKADPPSCD LQSAHGNPCM KEKLVMKTHC
260 270 280 290 300
PNDYQSAHYL NNDGKMASVK CPPKYGLTED CNFCRQMTGA SLKKGSYPLQ
310 320 330 340 350
DLFCQSSEDD GSKLKTKMKG VCEVGVQAHK KCDGQLSTAH EVVPFAVFKN
360 370 380 390 400
SKKVYLDKLD LKTEENLLPD SFVCFEHKGQ YKGTMDSGQT KRELKSFDIS
410 420 430 440 450
QCPKIGGHGS KKCTGDAAFC SAYECTAQYA NAYCSHANGS GIVQIQVSGV
460 470 480 490 500
WKKPLCVGYE RVVVKRELSA KPIQRVEPCT TCITKCEPHG LVVRSTGFKI
510 520 530 540 550
SSAVACASGV CVTGSQSPST EITLKYPGIS QSSGGDIGVH MAHDDQSVSS
560 570 580 590 600
KIVAHCPPQD PCLVHGCIVC AHGLINYQCH TALSAFVVVF VFSSIAIICL
610 620 630 640 650
AVLYRVLKCL KIAPRKVLNP LMWITAFIRW IYKKMVARVA HNINQVNREI
660 670 680 690 700
GWMEGGQLVL GNPAPIPRHA PIPRYSTYLM LLLIVSYASA CSELIQASSR
710 720 730 740 750
ITTCSTEGVN TKCRLSGTAL IRAGSVGAEA CLMLKGVKED QTKFLKIKTV
760 770 780 790 800
SSELSCREGQ SYWTGSISPK CLSSRRCHLV GECHVNRCLS WRDNETSAEF
810 820 830 840 850
SFVGESTTMR ENKCFEQCGG WGCGCFNVNP SCLFVHTYLQ SVRKEALRVF
860 870 880 890 900
NCIDWVHKLT LEITDFDGSV STIDLGASSS RFTNWGSVSL SLDAEGISGS
910 920 930 940 950
NSFSFIESPS KGYAIVDEPF SEIPRQGFLG EIRCNSESSV LSAHESCLRA
960 970 980 990 1000
PNLISYKPMI DQLECTTNLI DPFVVFERGS LPQTRNDKTF AASKGNRGVQ
1010 1020 1030 1040 1050
AFSKGSVQAD LTLMFDNFEV DFVGAAVSCD AAFLNLTGCY SCNAGARVCL
1060 1070 1080 1090 1100
SITSTGTGSL SAHNKDGSLH IVLPSENGTK DQCQILHFTV PEVEEEFMYS
1110 1120 1130 1140 1150
CDGDERPLLV KGTLIAIDPF DDRREAGGES TVVNPKSGSW NFFDWFSGLM
1160 1170 1180 1190 1200
SWFGGPLKLY SSFACMLHYQ LGSFSSLYIL EEQASLKCGL LPLRRPHRSV

RVKVIC
Length:1,206
Mass (Da):132,053
Last modified:November 1, 1995 - v2
Checksum:iD2E8017179285924
GO
Isoform NSm proteinBy similarity (identifier: P03518-2) [UniParc]FASTAAdd to basket
Also known as: P14

The sequence of this isoform differs from the canonical sequence as follows:
     1-38: Missing.
     154-1197: Missing.

Show »
Length:124
Mass (Da):13,657
Checksum:iE20CCDB67F1AD7DC
GO
Isoform NSm' proteinBy similarity (identifier: P03518-3) [UniParc]FASTAAdd to basket
Also known as: P13

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.
     154-1197: Missing.

Show »
Length:111
Mass (Da):12,110
Checksum:iF6418D10C3EC8F6D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0579851 – 51Missing in isoform NSm' protein. Add BLAST51
Alternative sequenceiVSP_0579861 – 38Missing in isoform NSm protein. Add BLAST38
Alternative sequenceiVSP_057987154 – 1197Missing in isoform NSm protein and isoform NSm' protein. Add BLAST1044

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M11157 Genomic RNA Translation: AAA47450.1
PIRiA04110 VGVURV

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiGP_RVFV
AccessioniPrimary (citable) accession number: P03518
Secondary accession number(s): Q86494, Q86495
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 87 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure
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Main funding by: National Institutes of Health