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Protein

Non-structural protein NS-S

Gene

N

Organism
Bunyavirus snowshoe hare
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Inhibits host transcriptional machinery, by producing modifications to the phosphorylation state of the C-terminal domain (CTD) of RNA polymerase II. Inhibits phosphorylation at serine 2 in the heptapeptide repeat (YSPTSPS) of the CTD of RNA polymerase II, suggesting that the elongation step of transcription and/or 3'-end processing is prevented. Inhibition of host transcription machinery leads to shut off of host cell protein synthesis and inhibition of the host innate immune response. NSs also seems to be involved in the nuclear relocalization of host PABP1 (By similarity).By similarity

GO - Biological processi

  1. suppression by virus of host gene expression Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of eukaryotic host transcription initiation by virus

Names & Taxonomyi

Protein namesi
Recommended name:
Non-structural protein NS-S
Gene namesi
Name:N
OrganismiBunyavirus snowshoe hare
Taxonomic identifieri11580 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeOrthobunyavirus
Virus hostiAedes [TaxID: 7158]
Bos taurus (Bovine) [TaxID: 9913]
Canis familiaris (Dog) (Canis lupus familiaris) [TaxID: 9615]
Culex [TaxID: 7174]
Culiseta [TaxID: 174825]
Equus caballus (Horse) [TaxID: 9796]
Gallus gallus (Chicken) [TaxID: 9031]
Homo sapiens (Human) [TaxID: 9606]
Lepus americanus (Snowshoe hare) [TaxID: 48086]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9292Non-structural protein NS-SPRO_0000221975Add
BLAST

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR000797. Bunya_NSs.
[Graphical view]
PfamiPF01104. Bunya_NS-S. 1 hit.
[Graphical view]
PIRSFiPIRSF003954. NS-S_OrthobunV. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform NSS (identifier: P03514-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMSHQQVQMD LILMQGIWHS VLNMQNQSIL LQLGSSSSMP QRPRLLSRVS
60 70 80 90
QRGRQILNLE SGRWRLSIII FLETGTIQLT ATILPSTDCQ DI

Note: Produced by alternative initiation in the N gene, but encoded on another frame.

Length:92
Mass (Da):10,490
Last modified:July 21, 1986 - v1
Checksum:iB5CCF0C9FD4FA8C1
GO
Isoform N (identifier: P03513-1) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry P03513.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:235
Mass (Da):26,771
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02390 Genomic RNA. No translation available.
J02393 Genomic RNA. No translation available.
PIRiA04105. MNVUSH.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02390 Genomic RNA. No translation available.
J02393 Genomic RNA. No translation available.
PIRiA04105. MNVUSH.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR000797. Bunya_NSs.
[Graphical view]
PfamiPF01104. Bunya_NS-S. 1 hit.
[Graphical view]
PIRSFiPIRSF003954. NS-S_OrthobunV. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete sequence and coding content of snowshoe hare bunyavirus small (S) viral RNA species."
    Bishop D.H.L., Gould K.G., Akashi H., Clerx-Van Haaster C.M.
    Nucleic Acids Res. 10:3703-3713(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Nucleotide sequence analyses and predicted coding of bunyavirus genome RNA species."
    Clerx-Van Haaster C.M., Akashi H., Auperin D.D., Bishop D.H.L.
    J. Virol. 41:119-128(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-47.

Entry informationi

Entry nameiNSS_BUNSH
AccessioniPrimary (citable) accession number: P03514
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 1, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.