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P03508

- NEP_I34A1

UniProt

P03508 - NEP_I34A1

Protein

Nuclear export protein

Gene

NS

Organism
Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs to which the M1 protein is bound. Because the M1 protein is not available in large quantities until the later stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the nucleus before sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enters the cytoplasm only when they have associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. fusion of virus membrane with host plasma membrane Source: Reactome
    2. intracellular transport of virus Source: Reactome
    3. receptor-mediated endocytosis of virus by host cell Source: Reactome
    4. RNA export from nucleus Source: InterPro
    5. uncoating of virus Source: Reactome
    6. viral entry into host cell Source: Reactome
    7. viral genome maturation Source: Reactome
    8. viral genome packaging Source: Reactome
    9. viral life cycle Source: Reactome
    10. viral process Source: Reactome
    11. viral release from host cell Source: Reactome
    12. viral transcription Source: Reactome
    13. virion assembly Source: Reactome

    Keywords - Biological processi

    Host-virus interaction, Transport

    Enzyme and pathway databases

    ReactomeiREACT_6145. Influenza Life Cycle.
    REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
    REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
    REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
    REACT_6212. Budding.
    REACT_6235. Packaging of Eight RNA Segments.
    REACT_6321. Uncoating of the Influenza Virion.
    REACT_6326. Release.
    REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
    REACT_9491. Viral mRNA Translation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear export protein
    Short name:
    NEP
    Alternative name(s):
    Non-structural protein 2
    Short name:
    NS2
    Gene namesi
    Name:NS
    OrganismiInfluenza A virus (strain A/Puerto Rico/8/1934 H1N1)
    Taxonomic identifieri211044 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Homo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000009255: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. endocytic vesicle membrane Source: Reactome
    3. endosome lumen Source: Reactome
    4. extracellular region Source: Reactome
    5. host cell nucleus Source: UniProtKB-SubCell
    6. nucleoplasm Source: Reactome
    7. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 121121Nuclear export proteinPRO_0000079005Add
    BLAST

    Interactioni

    Subunit structurei

    Binds M1 protein. May interact with human nucleoporin RAB/HRB and exportin XPO1/CRM1.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MP034853EBI-2547979,EBI-2547543

    Protein-protein interaction databases

    IntActiP03508. 7 interactions.
    MINTiMINT-6376762.

    Structurei

    Secondary structure

    1
    121
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi64 – 8522
    Helixi94 – 11522

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PD3X-ray2.60A/B59-116[»]
    ProteinModelPortaliP03508.
    SMRiP03508. Positions 64-116.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03508.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi12 – 2110Nuclear export signal
    Motifi85 – 9410Nuclear export signalBy similarity

    Sequence similaritiesi

    Belongs to the influenza viruses NEP family.Curated

    Family and domain databases

    InterProiIPR000968. Flu_NS2.
    [Graphical view]
    PfamiPF00601. Flu_NS2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform NEP (identifier: P03508-1) [UniParc]FASTAAdd to Basket

    Also known as: NS2

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDPNTVSSFQ DILLRMSKMQ LESSSGDLNG MITQFESLKL YRDSLGEAVM    50
    RMGDLHSLQN RNEKWREQLG QKFEEIRWLI EEVRHKLKIT ENSFEQITFM 100
    QALHLLLEVE QEIRTFSFQL I 121
    Length:121
    Mass (Da):14,380
    Last modified:July 21, 1986 - v1
    Checksum:iABFAC522B4F14854
    GO
    Isoform NS1 (identifier: P03496-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry P03496.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:230
    Mass (Da):25,868
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 261G → E in AAA43535. (PubMed:7385583)Curated
    Sequence conflicti26 – 261G → E in ABO21704. (PubMed:15163504)Curated
    Sequence conflicti26 – 261G → E in ABD77681. 1 PublicationCurated
    Sequence conflicti89 – 891I → V in AAA43535. (PubMed:7385583)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01104 Genomic RNA. Translation: CAA24293.1.
    J02150 Genomic RNA. Translation: AAA43535.1.
    EF467817 Genomic RNA. Translation: ABO21704.1.
    AF389122 Genomic RNA. Translation: AAM75164.1.
    CY009448 Genomic RNA. Translation: ABD77681.1.
    RefSeqiNP_040983.1. NC_002020.1.

    Genome annotation databases

    GeneIDi956532.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01104 Genomic RNA. Translation: CAA24293.1 .
    J02150 Genomic RNA. Translation: AAA43535.1 .
    EF467817 Genomic RNA. Translation: ABO21704.1 .
    AF389122 Genomic RNA. Translation: AAM75164.1 .
    CY009448 Genomic RNA. Translation: ABD77681.1 .
    RefSeqi NP_040983.1. NC_002020.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1PD3 X-ray 2.60 A/B 59-116 [» ]
    ProteinModelPortali P03508.
    SMRi P03508. Positions 64-116.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P03508. 7 interactions.
    MINTi MINT-6376762.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 956532.

    Enzyme and pathway databases

    Reactomei REACT_6145. Influenza Life Cycle.
    REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
    REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
    REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
    REACT_6212. Budding.
    REACT_6235. Packaging of Eight RNA Segments.
    REACT_6321. Uncoating of the Influenza Virion.
    REACT_6326. Release.
    REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
    REACT_9491. Viral mRNA Translation.

    Miscellaneous databases

    EvolutionaryTracei P03508.

    Family and domain databases

    InterProi IPR000968. Flu_NS2.
    [Graphical view ]
    Pfami PF00601. Flu_NS2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of the influenza A/PR/8/34 virus NS gene and comparison with the NS genes of the A/Udorn/72 and A/FPV/Rostock/34 strains."
      Baez M., Taussig R., Zazra J.J., Young J.F., Palese P., Reisfeld A., Skalka A.M.
      Nucleic Acids Res. 8:5845-5858(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Gene 8 of influenza virus: sequences of cDNA transcribed from the 3' ends of viral RNA of influenza A and B strains."
      Air G.M., Hackett J.A.
      Virology 103:291-298(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    3. "Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments."
      de Wit E., Spronken M.I.J., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D.M.E., Fouchier R.A.M.
      Virus Res. 103:155-161(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], REVERSE GENETICS.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    6. "Molecular assembly of influenza virus: association of the NS2 protein with virion matrix."
      Yasuda J., Nakada S., Kato A., Toyoda T., Ishihama A.
      Virology 196:249-255(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH M1 PROTEIN.
    7. "The influenza virus NEP (NS2 protein) mediates the nuclear export of viral ribonucleoproteins."
      O'Neill R.E., Talon J., Palese P.
      EMBO J. 17:288-296(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HUMAN RAB/HRB.
    8. "Crystal structure of the M1 protein-binding domain of the influenza A virus nuclear export protein (NEP/NS2)."
      Akarsu H., Burmeister W.P., Petosa C., Petit I., Muller C.W., Ruigrok R.W., Baudin F.
      EMBO J. 22:4646-4655(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 59-116.

    Entry informationi

    Entry nameiNEP_I34A1
    AccessioniPrimary (citable) accession number: P03508
    Secondary accession number(s): A4GXG8
    , Q20N33, Q67266, Q71QT4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Average number present in a viral particle is estimated to be 130-200 molecules.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3