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Protein

Nuclear export protein

Gene

NS

Organism
Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs bound to the protein M1. Since protein M1 is not available in large quantities before late stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the host nucleus until sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enter the host cytoplasm only when associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced.UniRule annotation

Miscellaneous

Average number present in a viral particle is estimated to be 130-200 molecules.

GO - Biological processi

Keywordsi

Biological processHost-virus interaction, Transport

Enzyme and pathway databases

ReactomeiR-HSA-168255. Influenza Life Cycle.
R-HSA-168275. Entry of Influenza Virion into Host Cell via Endocytosis.
R-HSA-168288. Fusion of the Influenza Virion to the Host Cell Endosome.
R-HSA-168298. Release.
R-HSA-168302. Budding.
R-HSA-168303. Packaging of Eight RNA Segments.
R-HSA-168330. Viral RNP Complexes in the Host Cell Nucleus.
R-HSA-168333. NEP/NS2 Interacts with the Cellular Export Machinery.
R-HSA-168336. Uncoating of the Influenza Virion.
R-HSA-192823. Viral mRNA Translation.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear export proteinUniRule annotation
Short name:
NEPUniRule annotation
Alternative name(s):
Non-structural protein 2UniRule annotation
Short name:
NS2UniRule annotation
Gene namesi
Name:NSUniRule annotation
OrganismiInfluenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Taxonomic identifieri211044 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
Proteomesi
  • UP000116373 Componenti: Genome
  • UP000170967 Componenti: Genome
  • UP000009255 Componenti: Genome
  • UP000109386 Componenti: Genome

Subcellular locationi

  • Virion UniRule annotation
  • Host nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000790051 – 121Nuclear export proteinAdd BLAST121

Interactioni

Subunit structurei

Interacts with protein M1. May interact with host nucleoporin RAB/HRB and exportin XPO1/CRM1.UniRule annotation

Binary interactionsi

Show more details

Protein-protein interaction databases

IntActiP03508. 60 interactors.
MINTiMINT-6376762.

Structurei

Secondary structure

1121
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi64 – 85Combined sources22
Helixi94 – 115Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PD3X-ray2.60A/B59-116[»]
ProteinModelPortaliP03508.
SMRiP03508.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03508.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 21Nuclear export signalUniRule annotation10
Motifi85 – 94Nuclear export signalUniRule annotation10

Sequence similaritiesi

Belongs to the influenza viruses NEP family.UniRule annotation

Phylogenomic databases

KOiK19395.
OrthoDBiVOG090000NT.

Family and domain databases

HAMAPiMF_04067. INFV_NEP. 1 hit.
InterProiView protein in InterPro
IPR000968. Flu_NS2.
PfamiView protein in Pfam
PF00601. Flu_NS2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform NEP (identifier: P03508-1) [UniParc]FASTAAdd to basket
Also known as: NS2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPNTVSSFQ DILLRMSKMQ LESSSGDLNG MITQFESLKL YRDSLGEAVM
60 70 80 90 100
RMGDLHSLQN RNEKWREQLG QKFEEIRWLI EEVRHKLKIT ENSFEQITFM
110 120
QALHLLLEVE QEIRTFSFQL I
Length:121
Mass (Da):14,380
Last modified:July 21, 1986 - v1
Checksum:iABFAC522B4F14854
GO
Isoform NS1 (identifier: P03496-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P03496.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:230
Mass (Da):25,868
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26G → E in AAA43535 (PubMed:7385583).1
Sequence conflicti26G → E in ABO21704 (PubMed:15163504).1
Sequence conflicti26G → E in ABD77681 (Ref. 5) 1
Sequence conflicti89I → V in AAA43535 (PubMed:7385583).1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01104 Genomic RNA. Translation: CAA24293.1.
J02150 Genomic RNA. Translation: AAA43535.1.
EF467817 Genomic RNA. Translation: ABO21704.1.
AF389122 Genomic RNA. Translation: AAM75164.1.
CY009448 Genomic RNA. Translation: ABD77681.1.
RefSeqiNP_040983.1. NC_002020.1.

Genome annotation databases

GeneIDi956532.
KEGGivg:956532.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNEP_I34A1
AccessioniPrimary (citable) accession number: P03508
Secondary accession number(s): A4GXG8
, Q20N33, Q67266, Q71QT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 7, 2017
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families