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P03508

- NEP_I34A1

UniProt

P03508 - NEP_I34A1

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Protein

Nuclear export protein

Gene

NS

Organism
Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs to which the M1 protein is bound. Because the M1 protein is not available in large quantities until the later stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the nucleus before sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enters the cytoplasm only when they have associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced.

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: Reactome
  2. intracellular transport of virus Source: Reactome
  3. receptor-mediated endocytosis of virus by host cell Source: Reactome
  4. RNA export from nucleus Source: InterPro
  5. uncoating of virus Source: Reactome
  6. viral entry into host cell Source: Reactome
  7. viral genome maturation Source: Reactome
  8. viral genome packaging Source: Reactome
  9. viral life cycle Source: Reactome
  10. viral process Source: Reactome
  11. viral release from host cell Source: Reactome
  12. viral transcription Source: Reactome
  13. virion assembly Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Transport

Enzyme and pathway databases

ReactomeiREACT_6145. Influenza Life Cycle.
REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
REACT_6212. Budding.
REACT_6235. Packaging of Eight RNA Segments.
REACT_6321. Uncoating of the Influenza Virion.
REACT_6326. Release.
REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
REACT_9491. Viral mRNA Translation.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear export protein
Short name:
NEP
Alternative name(s):
Non-structural protein 2
Short name:
NS2
Gene namesi
Name:NS
OrganismiInfluenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Taxonomic identifieri211044 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000009255: Genome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. endocytic vesicle membrane Source: Reactome
  3. endosome lumen Source: Reactome
  4. extracellular region Source: Reactome
  5. host cell nucleus Source: UniProtKB-KW
  6. nucleoplasm Source: Reactome
  7. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 121121Nuclear export proteinPRO_0000079005Add
BLAST

Interactioni

Subunit structurei

Binds M1 protein. May interact with human nucleoporin RAB/HRB and exportin XPO1/CRM1.

Binary interactionsi

WithEntry#Exp.IntActNotes
MP034853EBI-2547979,EBI-2547543

Protein-protein interaction databases

IntActiP03508. 7 interactions.
MINTiMINT-6376762.

Structurei

Secondary structure

1
121
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi64 – 8522
Helixi94 – 11522

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PD3X-ray2.60A/B59-116[»]
ProteinModelPortaliP03508.
SMRiP03508. Positions 64-116.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03508.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 2110Nuclear export signal
Motifi85 – 9410Nuclear export signalBy similarity

Sequence similaritiesi

Belongs to the influenza viruses NEP family.Curated

Family and domain databases

InterProiIPR000968. Flu_NS2.
[Graphical view]
PfamiPF00601. Flu_NS2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform NEP (identifier: P03508-1) [UniParc]FASTAAdd to Basket

Also known as: NS2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPNTVSSFQ DILLRMSKMQ LESSSGDLNG MITQFESLKL YRDSLGEAVM
60 70 80 90 100
RMGDLHSLQN RNEKWREQLG QKFEEIRWLI EEVRHKLKIT ENSFEQITFM
110 120
QALHLLLEVE QEIRTFSFQL I
Length:121
Mass (Da):14,380
Last modified:July 21, 1986 - v1
Checksum:iABFAC522B4F14854
GO
Isoform NS1 (identifier: P03496-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry P03496.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:230
Mass (Da):25,868
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261G → E in AAA43535. (PubMed:7385583)Curated
Sequence conflicti26 – 261G → E in ABO21704. (PubMed:15163504)Curated
Sequence conflicti26 – 261G → E in ABD77681. 1 PublicationCurated
Sequence conflicti89 – 891I → V in AAA43535. (PubMed:7385583)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01104 Genomic RNA. Translation: CAA24293.1.
J02150 Genomic RNA. Translation: AAA43535.1.
EF467817 Genomic RNA. Translation: ABO21704.1.
AF389122 Genomic RNA. Translation: AAM75164.1.
CY009448 Genomic RNA. Translation: ABD77681.1.
RefSeqiNP_040983.1. NC_002020.1.

Genome annotation databases

GeneIDi956532.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01104 Genomic RNA. Translation: CAA24293.1 .
J02150 Genomic RNA. Translation: AAA43535.1 .
EF467817 Genomic RNA. Translation: ABO21704.1 .
AF389122 Genomic RNA. Translation: AAM75164.1 .
CY009448 Genomic RNA. Translation: ABD77681.1 .
RefSeqi NP_040983.1. NC_002020.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PD3 X-ray 2.60 A/B 59-116 [» ]
ProteinModelPortali P03508.
SMRi P03508. Positions 64-116.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P03508. 7 interactions.
MINTi MINT-6376762.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 956532.

Enzyme and pathway databases

Reactomei REACT_6145. Influenza Life Cycle.
REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
REACT_6212. Budding.
REACT_6235. Packaging of Eight RNA Segments.
REACT_6321. Uncoating of the Influenza Virion.
REACT_6326. Release.
REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
REACT_9491. Viral mRNA Translation.

Miscellaneous databases

EvolutionaryTracei P03508.

Family and domain databases

InterProi IPR000968. Flu_NS2.
[Graphical view ]
Pfami PF00601. Flu_NS2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence of the influenza A/PR/8/34 virus NS gene and comparison with the NS genes of the A/Udorn/72 and A/FPV/Rostock/34 strains."
    Baez M., Taussig R., Zazra J.J., Young J.F., Palese P., Reisfeld A., Skalka A.M.
    Nucleic Acids Res. 8:5845-5858(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Gene 8 of influenza virus: sequences of cDNA transcribed from the 3' ends of viral RNA of influenza A and B strains."
    Air G.M., Hackett J.A.
    Virology 103:291-298(1980) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments."
    de Wit E., Spronken M.I.J., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D.M.E., Fouchier R.A.M.
    Virus Res. 103:155-161(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], REVERSE GENETICS.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  6. "Molecular assembly of influenza virus: association of the NS2 protein with virion matrix."
    Yasuda J., Nakada S., Kato A., Toyoda T., Ishihama A.
    Virology 196:249-255(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH M1 PROTEIN.
  7. "The influenza virus NEP (NS2 protein) mediates the nuclear export of viral ribonucleoproteins."
    O'Neill R.E., Talon J., Palese P.
    EMBO J. 17:288-296(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN RAB/HRB.
  8. "Crystal structure of the M1 protein-binding domain of the influenza A virus nuclear export protein (NEP/NS2)."
    Akarsu H., Burmeister W.P., Petosa C., Petit I., Muller C.W., Ruigrok R.W., Baudin F.
    EMBO J. 22:4646-4655(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 59-116.

Entry informationi

Entry nameiNEP_I34A1
AccessioniPrimary (citable) accession number: P03508
Secondary accession number(s): A4GXG8
, Q20N33, Q67266, Q71QT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 29, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Average number present in a viral particle is estimated to be 130-200 molecules.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3