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P03508 (NEP_I34A1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear export protein

Short name=NEP
Alternative name(s):
Non-structural protein 2
Short name=NS2
Gene names
Name:NS
OrganismInfluenza A virus (strain A/Puerto Rico/8/1934 H1N1) [Reference proteome]
Taxonomic identifier211044 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length121 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs to which the M1 protein is bound. Because the M1 protein is not available in large quantities until the later stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the nucleus before sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enters the cytoplasm only when they have associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced.

Subunit structure

Binds M1 protein. May interact with human nucleoporin RAB/HRB and exportin XPO1/CRM1.

Subcellular location

Virion Potential. Host nucleus.

Miscellaneous

Average number present in a viral particle is estimated to be 130-200 molecules.

Sequence similarities

Belongs to the influenza viruses NEP family.

Ontologies

Keywords
   Biological processHost-virus interaction
Transport
   Cellular componentHost nucleus
Virion
   Coding sequence diversityAlternative splicing
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA export from nucleus

Inferred from electronic annotation. Source: InterPro

fusion of virus membrane with host plasma membrane

Traceable author statement. Source: Reactome

intracellular transport of virus

Traceable author statement. Source: Reactome

receptor-mediated endocytosis of virus by host cell

Traceable author statement. Source: Reactome

uncoating of virus

Traceable author statement. Source: Reactome

viral entry into host cell

Traceable author statement. Source: Reactome

viral genome maturation

Traceable author statement. Source: Reactome

viral genome packaging

Traceable author statement. Source: Reactome

viral life cycle

Traceable author statement. Source: Reactome

viral process

Traceable author statement. Source: Reactome

viral release from host cell

Traceable author statement. Source: Reactome

viral transcription

Traceable author statement. Source: Reactome

virion assembly

Traceable author statement. Source: Reactome

   Cellular_componentcytosol

Traceable author statement. Source: Reactome

endocytic vesicle membrane

Traceable author statement. Source: Reactome

endosome lumen

Traceable author statement. Source: Reactome

extracellular region

Traceable author statement. Source: Reactome

host cell nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleoplasm

Traceable author statement. Source: Reactome

virion

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MP034853EBI-2547979,EBI-2547543

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform NEP (identifier: P03508-1)

Also known as: NS2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform NS1 (identifier: P03496-1)

The sequence of this isoform can be found in the external entry P03496.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 121121Nuclear export protein
PRO_0000079005

Regions

Motif12 – 2110Nuclear export signal
Motif85 – 9410Nuclear export signal By similarity

Experimental info

Sequence conflict261G → E in AAA43535. Ref.2
Sequence conflict261G → E in ABO21704. Ref.3
Sequence conflict261G → E in ABD77681. Ref.5
Sequence conflict891I → V in AAA43535. Ref.2

Secondary structure

..... 121
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform NEP (NS2) [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: ABFAC522B4F14854

FASTA12114,380
        10         20         30         40         50         60 
MDPNTVSSFQ DILLRMSKMQ LESSSGDLNG MITQFESLKL YRDSLGEAVM RMGDLHSLQN 

        70         80         90        100        110        120 
RNEKWREQLG QKFEEIRWLI EEVRHKLKIT ENSFEQITFM QALHLLLEVE QEIRTFSFQL 


I 

« Hide

Isoform NS1 [UniParc].

See P03496.

References

[1]"Complete nucleotide sequence of the influenza A/PR/8/34 virus NS gene and comparison with the NS genes of the A/Udorn/72 and A/FPV/Rostock/34 strains."
Baez M., Taussig R., Zazra J.J., Young J.F., Palese P., Reisfeld A., Skalka A.M.
Nucleic Acids Res. 8:5845-5858(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Gene 8 of influenza virus: sequences of cDNA transcribed from the 3' ends of viral RNA of influenza A and B strains."
Air G.M., Hackett J.A.
Virology 103:291-298(1980) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]"Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments."
de Wit E., Spronken M.I.J., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D.M.E., Fouchier R.A.M.
Virus Res. 103:155-161(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], REVERSE GENETICS.
[4]"Plasmid-only rescue of influenza A virus vaccine candidates."
Schickli J.H., Flandorfer A., Nakaya T., Martinez-Sobrido L., Garcia-Sastre A., Palese P.
Philos. Trans. R. Soc. Lond., B, Biol. Sci. 356:1965-1973(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[5]"The NIAID influenza genome sequencing project."
Ghedin E., Spiro D., Miller N., Zaborsky J., Feldblyum T., Subbu V., Shumway M., Sparenborg J., Groveman L., Halpin R., Sitz J., Koo H., Salzberg S.L., Webster R.G., Hoffmann E., Krauss S., Naeve C., Bao Y. expand/collapse author list , Bolotov P., Dernovoy D., Kiryutin B., Lipman D.J., Tatusova T.
Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[6]"Molecular assembly of influenza virus: association of the NS2 protein with virion matrix."
Yasuda J., Nakada S., Kato A., Toyoda T., Ishihama A.
Virology 196:249-255(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH M1 PROTEIN.
[7]"The influenza virus NEP (NS2 protein) mediates the nuclear export of viral ribonucleoproteins."
O'Neill R.E., Talon J., Palese P.
EMBO J. 17:288-296(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HUMAN RAB/HRB.
[8]"Crystal structure of the M1 protein-binding domain of the influenza A virus nuclear export protein (NEP/NS2)."
Akarsu H., Burmeister W.P., Petosa C., Petit I., Muller C.W., Ruigrok R.W., Baudin F.
EMBO J. 22:4646-4655(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 59-116.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V01104 Genomic RNA. Translation: CAA24293.1.
J02150 Genomic RNA. Translation: AAA43535.1.
EF467817 Genomic RNA. Translation: ABO21704.1.
AF389122 Genomic RNA. Translation: AAM75164.1.
CY009448 Genomic RNA. Translation: ABD77681.1.
RefSeqNP_040983.1. NC_002020.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1PD3X-ray2.60A/B59-116[»]
ProteinModelPortalP03508.
SMRP03508. Positions 64-116.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP03508. 7 interactions.
MINTMINT-6376762.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID956532.

Enzyme and pathway databases

ReactomeREACT_116125. Disease.

Family and domain databases

InterProIPR000968. Flu_NS2.
[Graphical view]
PfamPF00601. Flu_NS2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP03508.

Entry information

Entry nameNEP_I34A1
AccessionPrimary (citable) accession number: P03508
Secondary accession number(s): A4GXG8 expand/collapse secondary AC list , Q20N33, Q67266, Q71QT4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references