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P03506 (NEP_I47A0) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear export protein

Short name=NEP
Alternative name(s):
Non-structural protein 2
Short name=NS2
Gene names
Name:NS
OrganismInfluenza A virus (strain A/Fort Monmouth/1/1947 H1N1)
Taxonomic identifier380282 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length121 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs to which the M1 protein is bound. Because the M1 protein is not available in large quantities until the later stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the nucleus before sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enters the cytoplasm only when they have associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced By similarity.

Subunit structure

Binds M1 protein. May interact with human nucleoporin RAB/HRB and exportin XPO1/CRM1 By similarity.

Subcellular location

Virion Potential. Host nucleus By similarity.

Miscellaneous

Average number present in a viral particle is estimated to be 130-200 molecules.

Sequence similarities

Belongs to the influenza viruses NEP family.

Ontologies

Keywords
   Biological processHost-virus interaction
Transport
   Cellular componentHost nucleus
Virion
   Coding sequence diversityAlternative splicing
Gene Ontology (GO)
   Biological_processRNA export from nucleus

Inferred from electronic annotation. Source: InterPro

viral process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

virion

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform NEP (identifier: P03506-1)

Also known as: NS2;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform NS1 (identifier: P03499-1)

The sequence of this isoform can be found in the external entry P03499.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 121121Nuclear export protein
PRO_0000078985

Regions

Motif12 – 2110Nuclear export signal By similarity
Motif85 – 9410Nuclear export signal By similarity

Natural variations

Natural variant311V → M in strain: A/Fort Monmouth/1/1947-MA.
Natural variant341L → Q in strain: A/Fort Monmouth/1/1947-MA.
Natural variant461G → E in strain: A/Fort Monmouth/1/1947-MA.
Natural variant50 – 523VRM → MRL in strain: A/Fort Monmouth/1/1947-MA.
Natural variant581L → F in strain: A/Fort Monmouth/1/1947-MA.
Natural variant1071F → L in strain: A/Fort Monmouth/1/1947-MA.

Sequences

Sequence LengthMass (Da)Tools
Isoform NEP (NS2) [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: ED67C64D21C9BE9F

FASTA12114,300
        10         20         30         40         50         60 
MDPNTVSSFQ DILMRMSKMQ LGSSSEDLNG VITLFESLKL YRDSLGEAVV RMGDLHSLQN 

        70         80         90        100        110        120 
RNGKWREQLG QKFEEIRWLI EEVRHRLKIT ENSFEQITFM QALQLLFEVE QEIRTFSFQL 


I 

« Hide

Isoform NS1 [UniParc].

See P03499.

References

[1]"Sequential mutations in the NS genes of influenza virus field strains."
Krystal M., Buonagurio D.A., Young J.F., Palese P.
J. Virol. 45:547-554(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: A/Fort Monmouth/1/1947-MA.
[2]"The influenza virus variant A/FM/1/47-MA possesses single amino acid replacements in the hemagglutinin, controlling virulence, and in the matrix protein, controlling virulence as well as growth."
Smeenk C.A., Brown E.G.
J. Virol. 68:530-534(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K00577 Genomic RNA. Translation: AAA43521.1.
U02087 Genomic RNA. Translation: AAA67340.1.
AJ238022 Genomic RNA. Translation: CAB50888.1.

3D structure databases

ProteinModelPortalP03506.
SMRP03506. Positions 63-116.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000968. Flu_NS2.
[Graphical view]
PfamPF00601. Flu_NS2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNEP_I47A0
AccessionPrimary (citable) accession number: P03506
Secondary accession number(s): Q77AL4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: February 19, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families