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P03506

- NEP_I47A0

UniProt

P03506 - NEP_I47A0

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Protein

Nuclear export protein

Gene

NS

Organism
Influenza A virus (strain A/Fort Monmouth/1/1947 H1N1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs to which the M1 protein is bound. Because the M1 protein is not available in large quantities until the later stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the nucleus before sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enters the cytoplasm only when they have associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced (By similarity).By similarity

GO - Biological processi

  1. RNA export from nucleus Source: InterPro
  2. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear export protein
Short name:
NEP
Alternative name(s):
Non-structural protein 2
Short name:
NS2
Gene namesi
Name:NS
OrganismiInfluenza A virus (strain A/Fort Monmouth/1/1947 H1N1)
Taxonomic identifieri380282 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

Virion Curated. Host nucleus By similarity

GO - Cellular componenti

  1. host cell nucleus Source: UniProtKB-KW
  2. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 121121Nuclear export proteinPRO_0000078985Add
BLAST

Interactioni

Subunit structurei

Binds M1 protein. May interact with human nucleoporin RAB/HRB and exportin XPO1/CRM1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP03506.
SMRiP03506. Positions 63-116.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 2110Nuclear export signalBy similarity
Motifi85 – 9410Nuclear export signalBy similarity

Sequence similaritiesi

Belongs to the influenza viruses NEP family.Curated

Family and domain databases

InterProiIPR000968. Flu_NS2.
[Graphical view]
PfamiPF00601. Flu_NS2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform NEP (identifier: P03506-1) [UniParc]FASTAAdd to Basket

Also known as: NS2

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPNTVSSFQ DILMRMSKMQ LGSSSEDLNG VITLFESLKL YRDSLGEAVV
60 70 80 90 100
RMGDLHSLQN RNGKWREQLG QKFEEIRWLI EEVRHRLKIT ENSFEQITFM
110 120
QALQLLFEVE QEIRTFSFQL I
Length:121
Mass (Da):14,300
Last modified:July 21, 1986 - v1
Checksum:iED67C64D21C9BE9F
GO
Isoform NS1 (identifier: P03499-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry P03499.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:202
Mass (Da):22,680
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti31 – 311V → M in strain: A/Fort Monmouth/1/1947-MA.
Natural varianti34 – 341L → Q in strain: A/Fort Monmouth/1/1947-MA.
Natural varianti46 – 461G → E in strain: A/Fort Monmouth/1/1947-MA.
Natural varianti50 – 523VRM → MRL in strain: A/Fort Monmouth/1/1947-MA.
Natural varianti58 – 581L → F in strain: A/Fort Monmouth/1/1947-MA.
Natural varianti107 – 1071F → L in strain: A/Fort Monmouth/1/1947-MA.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K00577 Genomic RNA. Translation: AAA43521.1.
U02087 Genomic RNA. Translation: AAA67340.1.
AJ238022 Genomic RNA. Translation: CAB50888.1.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K00577 Genomic RNA. Translation: AAA43521.1 .
U02087 Genomic RNA. Translation: AAA67340.1 .
AJ238022 Genomic RNA. Translation: CAB50888.1 .

3D structure databases

ProteinModelPortali P03506.
SMRi P03506. Positions 63-116.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR000968. Flu_NS2.
[Graphical view ]
Pfami PF00601. Flu_NS2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequential mutations in the NS genes of influenza virus field strains."
    Krystal M., Buonagurio D.A., Young J.F., Palese P.
    J. Virol. 45:547-554(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: A/Fort Monmouth/1/1947-MA.
  2. "The influenza virus variant A/FM/1/47-MA possesses single amino acid replacements in the hemagglutinin, controlling virulence, and in the matrix protein, controlling virulence as well as growth."
    Smeenk C.A., Brown E.G.
    J. Virol. 68:530-534(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiNEP_I47A0
AccessioniPrimary (citable) accession number: P03506
Secondary accession number(s): Q77AL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Average number present in a viral particle is estimated to be 130-200 molecules.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3