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P03506

- NEP_I47A0

UniProt

P03506 - NEP_I47A0

Protein

Nuclear export protein

Gene

NS

Organism
Influenza A virus (strain A/Fort Monmouth/1/1947 H1N1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
  1. Functioni

    Mediates the nuclear export of encapsidated genomic RNAs (ribonucleoproteins, RNPs). Acts as an adapter between viral RNPs complexes and the nuclear export machinery of the cell. Possesses no intrinsic RNA-binding activity, but includes a C-terminal M1-binding domain. This domain is believed to allow recognition of RNPs to which the M1 protein is bound. Because the M1 protein is not available in large quantities until the later stages of infection, such an indirect recognition mechanism probably ensures that genomic RNPs are not exported from the nucleus before sufficient quantities of viral mRNA and progeny genomic RNA have been synthesized. Furthermore, the RNPs enters the cytoplasm only when they have associated with the M1 protein that is necessary to guide them to the plasma membrane. May down-regulate viral RNA synthesis when overproduced By similarity.By similarity

    GO - Biological processi

    1. RNA export from nucleus Source: InterPro
    2. viral process Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear export protein
    Short name:
    NEP
    Alternative name(s):
    Non-structural protein 2
    Short name:
    NS2
    Gene namesi
    Name:NS
    OrganismiInfluenza A virus (strain A/Fort Monmouth/1/1947 H1N1)
    Taxonomic identifieri380282 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Homo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]

    Subcellular locationi

    Virion Curated. Host nucleus By similarity

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell
    2. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host nucleus, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 121121Nuclear export proteinPRO_0000078985Add
    BLAST

    Interactioni

    Subunit structurei

    Binds M1 protein. May interact with human nucleoporin RAB/HRB and exportin XPO1/CRM1 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP03506.
    SMRiP03506. Positions 63-116.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi12 – 2110Nuclear export signalBy similarity
    Motifi85 – 9410Nuclear export signalBy similarity

    Sequence similaritiesi

    Belongs to the influenza viruses NEP family.Curated

    Family and domain databases

    InterProiIPR000968. Flu_NS2.
    [Graphical view]
    PfamiPF00601. Flu_NS2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform NEP (identifier: P03506-1) [UniParc]FASTAAdd to Basket

    Also known as: NS2

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDPNTVSSFQ DILMRMSKMQ LGSSSEDLNG VITLFESLKL YRDSLGEAVV    50
    RMGDLHSLQN RNGKWREQLG QKFEEIRWLI EEVRHRLKIT ENSFEQITFM 100
    QALQLLFEVE QEIRTFSFQL I 121
    Length:121
    Mass (Da):14,300
    Last modified:July 21, 1986 - v1
    Checksum:iED67C64D21C9BE9F
    GO
    Isoform NS1 (identifier: P03499-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry P03499.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:202
    Mass (Da):22,680
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti31 – 311V → M in strain: A/Fort Monmouth/1/1947-MA.
    Natural varianti34 – 341L → Q in strain: A/Fort Monmouth/1/1947-MA.
    Natural varianti46 – 461G → E in strain: A/Fort Monmouth/1/1947-MA.
    Natural varianti50 – 523VRM → MRL in strain: A/Fort Monmouth/1/1947-MA.
    Natural varianti58 – 581L → F in strain: A/Fort Monmouth/1/1947-MA.
    Natural varianti107 – 1071F → L in strain: A/Fort Monmouth/1/1947-MA.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K00577 Genomic RNA. Translation: AAA43521.1.
    U02087 Genomic RNA. Translation: AAA67340.1.
    AJ238022 Genomic RNA. Translation: CAB50888.1.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K00577 Genomic RNA. Translation: AAA43521.1 .
    U02087 Genomic RNA. Translation: AAA67340.1 .
    AJ238022 Genomic RNA. Translation: CAB50888.1 .

    3D structure databases

    ProteinModelPortali P03506.
    SMRi P03506. Positions 63-116.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR000968. Flu_NS2.
    [Graphical view ]
    Pfami PF00601. Flu_NS2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequential mutations in the NS genes of influenza virus field strains."
      Krystal M., Buonagurio D.A., Young J.F., Palese P.
      J. Virol. 45:547-554(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
      Strain: A/Fort Monmouth/1/1947-MA.
    2. "The influenza virus variant A/FM/1/47-MA possesses single amino acid replacements in the hemagglutinin, controlling virulence, and in the matrix protein, controlling virulence as well as growth."
      Smeenk C.A., Brown E.G.
      J. Virol. 68:530-534(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiNEP_I47A0
    AccessioniPrimary (citable) accession number: P03506
    Secondary accession number(s): Q77AL4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Average number present in a viral particle is estimated to be 130-200 molecules.

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3