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P03465

- HEMA_INCCA

UniProt

P03465 - HEMA_INCCA

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Protein

Hemagglutinin-esterase-fusion glycoprotein

Gene
HE
Organism
Influenza C virus (strain C/California/1978)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell By similarity.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei71 – 711Nucleophile By similarity
Active sitei365 – 3651Charge relay system By similarity
Active sitei368 – 3681Charge relay system By similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  2. fusion of virus membrane with host plasma membrane Source: InterPro
  3. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase-fusion glycoprotein (EC:3.1.1.53)
Short name:
HEF
Cleaved into the following 2 chains:
Gene namesi
Name:HE
OrganismiInfluenza C virus (strain C/California/1978)
Taxonomic identifieri203224 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus C
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini15 – 629615Extracellular Reviewed predictionAdd
BLAST
Transmembranei630 – 65021Helical; Reviewed predictionAdd
BLAST
Topological domaini651 – 6544Cytoplasmic Reviewed prediction

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1414 By similarityAdd
BLAST
Chaini15 – 444430Hemagglutinin-esterase-fusion glycoprotein chain 1PRO_0000039144Add
BLAST
Chaini446 – 654209Hemagglutinin-esterase-fusion glycoprotein chain 2PRO_0000039145Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi20 ↔ 582Interchain (between HEF1 and HEF2 chains) By similarity
Glycosylationi26 – 261N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi61 – 611N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi119 ↔ 164 By similarity
Disulfide bondi139 ↔ 187 By similarity
Glycosylationi143 – 1431N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi209 ↔ 251 By similarity
Disulfide bondi228 ↔ 315 By similarity
Disulfide bondi236 ↔ 288 By similarity
Disulfide bondi345 ↔ 351 By similarity
Glycosylationi394 – 3941N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

In natural infection, inactive HEF is matured into HEF1 and HEF2 outside the cell by one or more trypsin-like, arginine-specific endoprotease By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer of disulfide-linked HEF1-HEF2 By similarity.

Structurei

3D structure databases

ProteinModelPortaliP03465.
SMRiP03465. Positions 15-440, 449-610.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni15 – 4026Fusion domain-1 By similarityAdd
BLAST
Regioni41 – 150110Esterase domain-1 By similarityAdd
BLAST
Regioni150 – 309160N-acetyl-9-O-acetylneuraminic acid binding By similarityAdd
BLAST
Regioni310 – 36455Esterase domain-2 By similarityAdd
BLAST
Regioni365 – 650286Fusion domain-2 By similarityAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.90.20.10. 1 hit.
InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013829. Hemagglutn_stalk.
IPR014831. Hemagglutn_stalk_influenz-C.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
PF08720. Hema_stalk. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03465-1 [UniParc]FASTAAdd to Basket

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MFFSLLLMLG LTEAEKIKIC LQKQVNSSFS LHNGFGGNLY ATEEKRMFEL    50
VKPKAGASVL NQSTWIGFGD SRTDQSNSAF PRSLMSAKTA DKFRSLSGGS 100
LMLSMFGPPG KVDYLYQGCG KHKVFYEGVN WSPHAAIDCY RKNWTDIKLN 150
FQKSIYELAS QSHCMSLVNA LDKTIPLQVT KGVAKNCNNS FLKNPALYTQ 200
EVKPLEQICG EENLAFFTLP TQFGTYECKL HLVASCYFIY DSKEVYNKRG 250
CGNYFQVIYD SSGKVVGGLD NRVSPYTGNS GDTPTMQCDM LQLKPGRYSV 300
RSSPRFLLMP ERSYCFDMKE KGPVTAVQSI WGKGRKSDYA VDQACLSTPG 350
CMLIQKQKPY IGEADDHHGD QEMRELLSGL DYEARCISQS GWVNETSPFT 400
EEYLLPPKFG RCPLAAKEES IPKIPDGLLI PTSGTDTTVT KPKSRIFGID 450
DLIIGLLFVA IVEAGIGGYL LGSRKESGGG VTKESAEKGF EKIGNDIQIL 500
RSSTNIAIEK LNDRISHDEQ AIRDLTLEIE NARSEALLGE LGIIRALLVG 550
NISIGLQESL WELASEITNR AGDLAVEVSP GCWIIDNNIC DQSCQNFIFK 600
FNETAPVPTI PPLDTKIDLQ SDPFYWGSSL GLAITAANLM AALVISGIAI 650
CRTK 654
Length:654
Mass (Da):72,085
Last modified:July 21, 1986 - v1
Checksum:i7F4C28D9EFD2E429
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K01689 Genomic RNA. Translation: AAA43791.1.
PIRiA04076. HMIVC8.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K01689 Genomic RNA. Translation: AAA43791.1 .
PIRi A04076. HMIVC8.

3D structure databases

ProteinModelPortali P03465.
SMRi P03465. Positions 15-440, 449-610.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.90.20.10. 1 hit.
InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013829. Hemagglutn_stalk.
IPR014831. Hemagglutn_stalk_influenz-C.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
PF08720. Hema_stalk. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Influenza C virus hemagglutinin: comparison with influenza A and B virus hemagglutinins."
    Nakada S., Creager R.S., Krystal M., Aaronson R.P., Palese P.
    J. Virol. 50:118-124(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_INCCA
AccessioniPrimary (citable) accession number: P03465
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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