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P03465

- HEMA_INCCA

UniProt

P03465 - HEMA_INCCA

Protein

Hemagglutinin-esterase-fusion glycoprotein

Gene

HE

Organism
Influenza C virus (strain C/California/1978)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell By similarity.By similarity

    Catalytic activityi

    N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei71 – 711NucleophileBy similarity
    Active sitei365 – 3651Charge relay systemBy similarity
    Active sitei368 – 3681Charge relay systemBy similarity

    GO - Molecular functioni

    1. sialate O-acetylesterase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
    2. fusion of virus membrane with host plasma membrane Source: InterPro
    3. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hemagglutinin, Hydrolase

    Keywords - Biological processi

    Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hemagglutinin-esterase-fusion glycoprotein (EC:3.1.1.53)
    Short name:
    HEF
    Cleaved into the following 2 chains:
    Gene namesi
    Name:HE
    OrganismiInfluenza C virus (strain C/California/1978)
    Taxonomic identifieri203224 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus C
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. viral envelope Source: UniProtKB-KW
    4. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1414By similarityAdd
    BLAST
    Chaini15 – 444430Hemagglutinin-esterase-fusion glycoprotein chain 1PRO_0000039144Add
    BLAST
    Chaini446 – 654209Hemagglutinin-esterase-fusion glycoprotein chain 2PRO_0000039145Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi20 ↔ 582Interchain (between HEF1 and HEF2 chains)By similarity
    Glycosylationi26 – 261N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi61 – 611N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi119 ↔ 164By similarity
    Disulfide bondi139 ↔ 187By similarity
    Glycosylationi143 – 1431N-linked (GlcNAc...); by hostSequence Analysis
    Disulfide bondi209 ↔ 251By similarity
    Disulfide bondi228 ↔ 315By similarity
    Disulfide bondi236 ↔ 288By similarity
    Disulfide bondi345 ↔ 351By similarity
    Glycosylationi394 – 3941N-linked (GlcNAc...); by hostSequence Analysis

    Post-translational modificationi

    In natural infection, inactive HEF is matured into HEF1 and HEF2 outside the cell by one or more trypsin-like, arginine-specific endoprotease.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homotrimer of disulfide-linked HEF1-HEF2.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP03465.
    SMRiP03465. Positions 15-440, 449-610.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini15 – 629615ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini651 – 6544CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei630 – 65021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni15 – 4026Fusion domain-1By similarityAdd
    BLAST
    Regioni41 – 150110Esterase domain-1By similarityAdd
    BLAST
    Regioni150 – 309160N-acetyl-9-O-acetylneuraminic acid bindingBy similarityAdd
    BLAST
    Regioni310 – 36455Esterase domain-2By similarityAdd
    BLAST
    Regioni365 – 650286Fusion domain-2By similarityAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di3.90.20.10. 1 hit.
    InterProiIPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    IPR013829. Hemagglutn_stalk.
    IPR014831. Hemagglutn_stalk_influenz-C.
    [Graphical view]
    PfamiPF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    PF08720. Hema_stalk. 1 hit.
    [Graphical view]
    SUPFAMiSSF49818. SSF49818. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P03465-1 [UniParc]FASTAAdd to Basket

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    MFFSLLLMLG LTEAEKIKIC LQKQVNSSFS LHNGFGGNLY ATEEKRMFEL    50
    VKPKAGASVL NQSTWIGFGD SRTDQSNSAF PRSLMSAKTA DKFRSLSGGS 100
    LMLSMFGPPG KVDYLYQGCG KHKVFYEGVN WSPHAAIDCY RKNWTDIKLN 150
    FQKSIYELAS QSHCMSLVNA LDKTIPLQVT KGVAKNCNNS FLKNPALYTQ 200
    EVKPLEQICG EENLAFFTLP TQFGTYECKL HLVASCYFIY DSKEVYNKRG 250
    CGNYFQVIYD SSGKVVGGLD NRVSPYTGNS GDTPTMQCDM LQLKPGRYSV 300
    RSSPRFLLMP ERSYCFDMKE KGPVTAVQSI WGKGRKSDYA VDQACLSTPG 350
    CMLIQKQKPY IGEADDHHGD QEMRELLSGL DYEARCISQS GWVNETSPFT 400
    EEYLLPPKFG RCPLAAKEES IPKIPDGLLI PTSGTDTTVT KPKSRIFGID 450
    DLIIGLLFVA IVEAGIGGYL LGSRKESGGG VTKESAEKGF EKIGNDIQIL 500
    RSSTNIAIEK LNDRISHDEQ AIRDLTLEIE NARSEALLGE LGIIRALLVG 550
    NISIGLQESL WELASEITNR AGDLAVEVSP GCWIIDNNIC DQSCQNFIFK 600
    FNETAPVPTI PPLDTKIDLQ SDPFYWGSSL GLAITAANLM AALVISGIAI 650
    CRTK 654
    Length:654
    Mass (Da):72,085
    Last modified:July 21, 1986 - v1
    Checksum:i7F4C28D9EFD2E429
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K01689 Genomic RNA. Translation: AAA43791.1.
    PIRiA04076. HMIVC8.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K01689 Genomic RNA. Translation: AAA43791.1 .
    PIRi A04076. HMIVC8.

    3D structure databases

    ProteinModelPortali P03465.
    SMRi P03465. Positions 15-440, 449-610.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.90.20.10. 1 hit.
    InterProi IPR008980. Capsid_hemagglutn.
    IPR007142. Hemagglutn-estrase_core.
    IPR003860. Hemagglutn-estrase_hemagglutn.
    IPR013829. Hemagglutn_stalk.
    IPR014831. Hemagglutn_stalk_influenz-C.
    [Graphical view ]
    Pfami PF03996. Hema_esterase. 1 hit.
    PF02710. Hema_HEFG. 1 hit.
    PF08720. Hema_stalk. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49818. SSF49818. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Influenza C virus hemagglutinin: comparison with influenza A and B virus hemagglutinins."
      Nakada S., Creager R.S., Krystal M., Aaronson R.P., Palese P.
      J. Virol. 50:118-124(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiHEMA_INCCA
    AccessioniPrimary (citable) accession number: P03465
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3