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P03455

- HEMA_I76AI

UniProt

P03455 - HEMA_I76AI

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Protein
Hemagglutinin
Gene
HA
Organism
Influenza A virus (strain A/Swine/New Jersey/11/1976 H1N1)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei344 – 3452Cleavage; by host By similarity

GO - Biological processi

  1. clathrin-mediated endocytosis of virus by host cell Source: UniProtKB-KW
  2. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  3. fusion of virus membrane with host plasma membrane Source: InterPro
  4. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin

Keywords - Biological processi

Clathrin- and caveolin-independent endocytosis of virus by host, Clathrin-mediated endocytosis of virus by host, Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin
Cleaved into the following 2 chains:
Gene namesi
Name:HA
OrganismiInfluenza A virus (strain A/Swine/New Jersey/11/1976 H1N1)
Taxonomic identifieri186460 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

Virion membrane; Single-pass type I membrane protein Reviewed prediction. Host apical cell membrane; Single-pass type I membrane protein
Note: Targeted to the apical plasma membrane in epithelial polarized cells through a signal present in the transmembrane domain. Associated with glycosphingolipid- and cholesterol-enriched detergent-resistant lipid rafts.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini18 – 529512Extracellular Reviewed prediction
Add
BLAST
Transmembranei530 – 55021Helical; Reviewed prediction
Add
BLAST
Topological domaini551 – 56616Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717 Reviewed prediction
Add
BLAST
Chaini18 – 343326Hemagglutinin HA1 chain
PRO_0000039081Add
BLAST
Chaini345 – 566222Hemagglutinin HA2 chain
PRO_0000039082Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi21 ↔ 481Interchain (between HA1 and HA2 chains) By similarity
Glycosylationi27 – 271N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi28 – 281N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi40 – 401N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi59 ↔ 292 By similarity
Disulfide bondi72 ↔ 84 By similarity
Glycosylationi104 – 1041N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi107 ↔ 153 By similarity
Disulfide bondi296 ↔ 320 By similarity
Glycosylationi304 – 3041N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi488 ↔ 492 By similarity
Glycosylationi498 – 4981N-linked (GlcNAc...); by host Reviewed prediction
Lipidationi555 – 5551S-palmitoyl cysteine; by host By similarity
Lipidationi562 – 5621S-palmitoyl cysteine; by host By similarity
Lipidationi565 – 5651S-palmitoyl cysteine; by host By similarity

Post-translational modificationi

In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. One identified protease that may be involved in this process is secreted in lungs by Clara cells By similarity.
Palmitoylated By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Interactioni

Subunit structurei

Homotrimer of disulfide-linked HA1-HA2.

Structurei

3D structure databases

ProteinModelPortaliP03455.
SMRiP03455. Positions 18-340, 345-519.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di2.10.77.10. 1 hit.
3.90.20.10. 1 hit.
3.90.209.20. 1 hit.
InterProiIPR008980. Capsid_hemagglutn.
IPR013828. Hemagglutn_HA1_a/b_dom.
IPR013827. Hemagglutn_HA1_b-rbn_dom.
IPR000149. Hemagglutn_influenz_A.
IPR001364. Hemagglutn_influenz_A/B.
IPR013829. Hemagglutn_stalk.
[Graphical view]
PfamiPF00509. Hemagglutinin. 1 hit.
[Graphical view]
PRINTSiPR00330. HEMAGGLUTN1.
PR00329. HEMAGGLUTN12.
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03455-1 [UniParc]FASTAAdd to Basket

« Hide

MKAILLVLLC TFAATNADTL CIGYHANNST DTVDTVLEKN VTVTHSVNLL    50
EDRHNGKLCK LGGIAPLHLG KCNIAGRLLG NPECELLLTV SSWSYIVETS 100
KSDNGTCYPG DFINYEELRE QLSSVSSFER FEIFPKTSSW PNHETNRGVT 150
AACPYAGANS FYRNLIWLVK KENSYPKLSK SYVNNKGKEV LVLWGIHHPP 200
TSTDQQSLYQ NADAYVFVGS SKYNRKFKPE IAARPKVRGQ AGRMSYYWTL 250
IEPGDTITFE ATGNLVVPRY AFAMNRGSGS GIIIWDAPVH DCNTKCQTPK 300
GAINTSLPFQ NIHPVTIGEC PKYVKSTKLR MATGLRNVPS IQSRGLFGAI 350
AGFIEGGWTG MIDGWYGYHH QNEQGSGYAA DQRSTQNAID GITNKVNSVI 400
EKMNTQFTAV GKEFNHLEKR IENLNKKVDD GFLDIWTYNA ELLVLLENER 450
TLDFHDSNVK NLYEKVRSQL RNNAKEIGNG CFEFYHKCDD TCMESVKNGT 500
YDYPKYSEES KLNREEIDGV KLESTRIYQI LAIYSTVASS LVLLVSLGAI 550
SFWMCSNGSL QCRICI 566
Length:566
Mass (Da):63,304
Last modified:July 21, 1986 - v1
Checksum:i035E1185BBC3A706
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K00992 Genomic RNA. Translation: AAB39851.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K00992 Genomic RNA. Translation: AAB39851.1 .

3D structure databases

ProteinModelPortali P03455.
SMRi P03455. Positions 18-340, 345-519.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.10.77.10. 1 hit.
3.90.20.10. 1 hit.
3.90.209.20. 1 hit.
InterProi IPR008980. Capsid_hemagglutn.
IPR013828. Hemagglutn_HA1_a/b_dom.
IPR013827. Hemagglutn_HA1_b-rbn_dom.
IPR000149. Hemagglutn_influenz_A.
IPR001364. Hemagglutn_influenz_A/B.
IPR013829. Hemagglutn_stalk.
[Graphical view ]
Pfami PF00509. Hemagglutinin. 1 hit.
[Graphical view ]
PRINTSi PR00330. HEMAGGLUTN1.
PR00329. HEMAGGLUTN12.
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Hemagglutinin of swine influenza virus: a single amino acid change pleiotropically affects viral antigenicity and replication."
    Both G.W., Shi C.H., Kilbourne E.D.
    Proc. Natl. Acad. Sci. U.S.A. 80:6996-7000(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_I76AI
AccessioniPrimary (citable) accession number: P03455
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: February 19, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Major glycoprotein, comprises over 80% of the envelope proteins present in virus particle.
The extent of infection into host organism is determined by HA. Influenza viruses bud from the apical surface of polarized epithelial cells (e.g. bronchial epithelial cells) into lumen of lungs and are therefore usually pneumotropic. The reason is that HA is cleaved by tryptase clara which is restricted to lungs. However, HAs of H5 and H7 pantropic avian viruses subtypes can be cleaved by furin and subtilisin-type enzymes, allowing the virus to grow in other organs than lungs.
The influenza A genome consist of 8 RNA segments. Genetic variation of hemagglutinin and/or neuraminidase genes results in the emergence of new influenza strains. The mechanism of variation can be the result of point mutations or the result of genetic reassortment between segments of two different strains.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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