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P03433

- PA_I34A1

UniProt

P03433 - PA_I34A1

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Protein

Polymerase acidic protein

Gene
PA
Organism
Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Implicated in endonuclease cleavage of capped RNA primers. Displays an elongation factor activity in viral RNA synthesis. Dispensable for viral transcription, but not replication.

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. RNA binding Source: InterPro
  3. RNA-directed RNA polymerase activity Source: InterPro

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: Reactome
  2. intracellular transport of virus Source: Reactome
  3. receptor-mediated endocytosis of virus by host cell Source: Reactome
  4. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
  5. transcription, DNA-templated Source: InterPro
  6. uncoating of virus Source: Reactome
  7. viral entry into host cell Source: Reactome
  8. viral genome maturation Source: Reactome
  9. viral genome packaging Source: Reactome
  10. viral life cycle Source: Reactome
  11. viral process Source: Reactome
  12. viral release from host cell Source: Reactome
  13. viral transcription Source: Reactome
  14. virion assembly Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus

Enzyme and pathway databases

ReactomeiREACT_6145. Influenza Life Cycle.
REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
REACT_6212. Budding.
REACT_6235. Packaging of Eight RNA Segments.
REACT_6248. Transport of Ribonucleoproteins into the Host Nucleus.
REACT_6321. Uncoating of the Influenza Virion.
REACT_6326. Release.
REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
REACT_6354. Viral Messenger RNA Synthesis.
REACT_9434. vRNP Assembly.
REACT_9491. Viral mRNA Translation.
REACT_9497. vRNA Synthesis.
REACT_9527. cRNA Synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase acidic protein
Alternative name(s):
RNA-directed RNA polymerase subunit P2
Gene namesi
Name:PA
OrganismiInfluenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Taxonomic identifieri211044 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000009255: Genome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. endosome lumen Source: Reactome
  3. extracellular region Source: Reactome
  4. nucleoplasm Source: Reactome
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 716716Polymerase acidic proteinPRO_0000078796Add
BLAST

Post-translational modificationi

Phosphorylated on serines and threonines by host kinases, including human casein kinase II By similarity.

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

Binary interactionsi

WithEntry#Exp.IntActNotes
MP034852EBI-2547616,EBI-2547543

Protein-protein interaction databases

DIPiDIP-43996N.
IntActiP03433. 35 interactions.
MINTiMINT-3375052.

Structurei

Secondary structure

1
716
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi273 – 2753
Beta strandi290 – 2945
Turni298 – 3014
Helixi303 – 3119
Beta strandi317 – 3248
Beta strandi327 – 3293
Helixi331 – 34515
Helixi364 – 3696
Helixi406 – 4149
Beta strandi419 – 4213
Helixi437 – 45014
Beta strandi451 – 4533
Helixi454 – 47421
Beta strandi477 – 4793
Beta strandi481 – 49010
Beta strandi496 – 50611
Beta strandi517 – 52610
Helixi530 – 5323
Turni534 – 5396
Beta strandi541 – 5488
Beta strandi559 – 57113
Helixi572 – 5798
Helixi580 – 5823
Helixi583 – 60220
Helixi608 – 6136
Beta strandi619 – 6246
Beta strandi627 – 6315
Helixi633 – 64917
Helixi653 – 67422
Helixi686 – 6916
Helixi698 – 71417

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZNLX-ray2.30A239-716[»]
ProteinModelPortaliP03433.
SMRiP03433. Positions 257-715.

Miscellaneous databases

EvolutionaryTraceiP03433.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR001009. RNA-dir_pol_influenzavirus.
[Graphical view]
PfamiPF00603. Flu_PA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

Isoform PA (identifier: P03433-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MEDFVRQCFN PMIVELAEKT MKEYGEDLKI ETNKFAAICT HLEVCFMYSD    50
FHFINEQGES IIVELGDPNA LLKHRFEIIE GRDRTMAWTV VNSICNTTGA 100
EKPKFLPDLY DYKENRFIEI GVTRREVHIY YLEKANKIKS EKTHIHIFSF 150
TGEEMATKAD YTLDEESRAR IKTRLFTIRQ EMASRGLWDS FRQSERGEET 200
IEERFEITGT MRKLADQSLP PNFSSLENFR AYVDGFEPNG YIEGKLSQMS 250
KEVNARIEPF LKTTPRPLRL PNGPPCSQRS KFLLMDALKL SIEDPSHEGE 300
GIPLYDAIKC MRTFFGWKEP NVVKPHEKGI NPNYLLSWKQ VLAELQDIEN 350
EEKIPKTKNM KKTSQLKWAL GENMAPEKVD FDDCKDVGDL KQYDSDEPEL 400
RSLASWIQNE FNKACELTDS SWIELDEIGE DVAPIEHIAS MRRNYFTSEV 450
SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKCR TKEGRRKTNL 500
YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEIGDMLI 550
RSAIGQVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES 600
SVKEKDMTKE FFENKSETWP IGESPKGVEE SSIGKVCRTL LAKSVFNSLY 650
ASPQLEGFSA ESRKLLLIVQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV 700
LLNASWFNSF LTHALS 716
Length:716
Mass (Da):82,588
Last modified:October 2, 2007 - v2
Checksum:i8829F53525814394
GO
Isoform PA-X (identifier: P0CK64-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry P0CK64.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:252
Mass (Da):29,423
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti550 – 5501I → L in CAA24295. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01106 Genomic RNA. Translation: CAA24295.1.
AF389117 Genomic RNA. Translation: AAM75157.1.
EF467820 Genomic RNA. Translation: ABO21708.1.
CY009449 Genomic RNA. Translation: ABD77682.1.
RefSeqiNP_040986.1. NC_002022.1.

Genome annotation databases

GeneIDi956535.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01106 Genomic RNA. Translation: CAA24295.1 .
AF389117 Genomic RNA. Translation: AAM75157.1 .
EF467820 Genomic RNA. Translation: ABO21708.1 .
CY009449 Genomic RNA. Translation: ABD77682.1 .
RefSeqi NP_040986.1. NC_002022.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2ZNL X-ray 2.30 A 239-716 [» ]
ProteinModelPortali P03433.
SMRi P03433. Positions 257-715.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-43996N.
IntActi P03433. 35 interactions.
MINTi MINT-3375052.

Chemistry

ChEMBLi CHEMBL1169598.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 956535.

Enzyme and pathway databases

Reactomei REACT_6145. Influenza Life Cycle.
REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
REACT_6212. Budding.
REACT_6235. Packaging of Eight RNA Segments.
REACT_6248. Transport of Ribonucleoproteins into the Host Nucleus.
REACT_6321. Uncoating of the Influenza Virion.
REACT_6326. Release.
REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
REACT_6354. Viral Messenger RNA Synthesis.
REACT_9434. vRNP Assembly.
REACT_9491. Viral mRNA Translation.
REACT_9497. vRNA Synthesis.
REACT_9527. cRNA Synthesis.

Miscellaneous databases

EvolutionaryTracei P03433.

Family and domain databases

InterProi IPR001009. RNA-dir_pol_influenzavirus.
[Graphical view ]
Pfami PF00603. Flu_PA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequences of influenza virus segments 1 and 3 reveal mosaic structure of a small viral RNA segment."
    Fields S., Winter G.
    Cell 28:303-313(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments."
    de Wit E., Spronken M.I.J., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D.M.E., Fouchier R.A.M.
    Virus Res. 103:155-161(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], REVERSE GENETICS.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  5. "The structural basis for an essential subunit interaction in influenza virus RNA polymerase."
    Obayashi E., Yoshida H., Kawai F., Shibayama N., Kawaguchi A., Nagata K., Tame J.R., Park S.Y.
    Nature 454:1127-1131(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 239-716.

Entry informationi

Entry nameiPA_I34A1
AccessioniPrimary (citable) accession number: P03433
Secondary accession number(s): A4GXH3, Q20N31, Q8JUU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 2, 2007
Last modified: September 3, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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