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P03433

- PA_I34A1

UniProt

P03433 - PA_I34A1

Protein

Polymerase acidic protein

Gene

PA

Organism
Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Implicated in endonuclease cleavage of capped RNA primers. Displays an elongation factor activity in viral RNA synthesis. Dispensable for viral transcription, but not replication.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. RNA binding Source: InterPro
    3. RNA-directed RNA polymerase activity Source: InterPro

    GO - Biological processi

    1. fusion of virus membrane with host plasma membrane Source: Reactome
    2. intracellular transport of virus Source: Reactome
    3. receptor-mediated endocytosis of virus by host cell Source: Reactome
    4. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
    5. transcription, DNA-templated Source: InterPro
    6. uncoating of virus Source: Reactome
    7. viral entry into host cell Source: Reactome
    8. viral genome maturation Source: Reactome
    9. viral genome packaging Source: Reactome
    10. viral life cycle Source: Reactome
    11. viral process Source: Reactome
    12. viral release from host cell Source: Reactome
    13. viral transcription Source: Reactome
    14. virion assembly Source: Reactome

    Keywords - Biological processi

    Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus

    Enzyme and pathway databases

    ReactomeiREACT_6145. Influenza Life Cycle.
    REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
    REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
    REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
    REACT_6212. Budding.
    REACT_6235. Packaging of Eight RNA Segments.
    REACT_6248. Transport of Ribonucleoproteins into the Host Nucleus.
    REACT_6321. Uncoating of the Influenza Virion.
    REACT_6326. Release.
    REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_9434. vRNP Assembly.
    REACT_9491. Viral mRNA Translation.
    REACT_9497. vRNA Synthesis.
    REACT_9527. cRNA Synthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polymerase acidic protein
    Alternative name(s):
    RNA-directed RNA polymerase subunit P2
    Gene namesi
    Name:PA
    OrganismiInfluenza A virus (strain A/Puerto Rico/8/1934 H1N1)
    Taxonomic identifieri211044 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Homo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000009255: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. endosome lumen Source: Reactome
    3. extracellular region Source: Reactome
    4. nucleoplasm Source: Reactome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 716716Polymerase acidic proteinPRO_0000078796Add
    BLAST

    Post-translational modificationi

    Phosphorylated on serines and threonines by host kinases, including human casein kinase II.By similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MP034852EBI-2547616,EBI-2547543

    Protein-protein interaction databases

    DIPiDIP-43996N.
    IntActiP03433. 35 interactions.
    MINTiMINT-3375052.

    Structurei

    Secondary structure

    1
    716
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi273 – 2753
    Beta strandi290 – 2945
    Turni298 – 3014
    Helixi303 – 3119
    Beta strandi317 – 3248
    Beta strandi327 – 3293
    Helixi331 – 34515
    Helixi364 – 3696
    Helixi406 – 4149
    Beta strandi419 – 4213
    Helixi437 – 45014
    Beta strandi451 – 4533
    Helixi454 – 47421
    Beta strandi477 – 4793
    Beta strandi481 – 49010
    Beta strandi496 – 50611
    Beta strandi517 – 52610
    Helixi530 – 5323
    Turni534 – 5396
    Beta strandi541 – 5488
    Beta strandi559 – 57113
    Helixi572 – 5798
    Helixi580 – 5823
    Helixi583 – 60220
    Helixi608 – 6136
    Beta strandi619 – 6246
    Beta strandi627 – 6315
    Helixi633 – 64917
    Helixi653 – 67422
    Helixi686 – 6916
    Helixi698 – 71417

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ZNLX-ray2.30A239-716[»]
    ProteinModelPortaliP03433.
    SMRiP03433. Positions 257-715.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03433.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the influenza viruses PA family.Curated

    Family and domain databases

    InterProiIPR001009. RNA-dir_pol_influenzavirus.
    [Graphical view]
    PfamiPF00603. Flu_PA. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by ribosomal frameshifting. Align

    Isoform PA (identifier: P03433-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEDFVRQCFN PMIVELAEKT MKEYGEDLKI ETNKFAAICT HLEVCFMYSD    50
    FHFINEQGES IIVELGDPNA LLKHRFEIIE GRDRTMAWTV VNSICNTTGA 100
    EKPKFLPDLY DYKENRFIEI GVTRREVHIY YLEKANKIKS EKTHIHIFSF 150
    TGEEMATKAD YTLDEESRAR IKTRLFTIRQ EMASRGLWDS FRQSERGEET 200
    IEERFEITGT MRKLADQSLP PNFSSLENFR AYVDGFEPNG YIEGKLSQMS 250
    KEVNARIEPF LKTTPRPLRL PNGPPCSQRS KFLLMDALKL SIEDPSHEGE 300
    GIPLYDAIKC MRTFFGWKEP NVVKPHEKGI NPNYLLSWKQ VLAELQDIEN 350
    EEKIPKTKNM KKTSQLKWAL GENMAPEKVD FDDCKDVGDL KQYDSDEPEL 400
    RSLASWIQNE FNKACELTDS SWIELDEIGE DVAPIEHIAS MRRNYFTSEV 450
    SHCRATEYIM KGVYINTALL NASCAAMDDF QLIPMISKCR TKEGRRKTNL 500
    YGFIIKGRSH LRNDTDVVNF VSMEFSLTDP RLEPHKWEKY CVLEIGDMLI 550
    RSAIGQVSRP MFLYVRTNGT SKIKMKWGME MRRCLLQSLQ QIESMIEAES 600
    SVKEKDMTKE FFENKSETWP IGESPKGVEE SSIGKVCRTL LAKSVFNSLY 650
    ASPQLEGFSA ESRKLLLIVQ ALRDNLEPGT FDLGGLYEAI EECLINDPWV 700
    LLNASWFNSF LTHALS 716
    Length:716
    Mass (Da):82,588
    Last modified:October 2, 2007 - v2
    Checksum:i8829F53525814394
    GO
    Isoform PA-X (identifier: P0CK64-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry P0CK64.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:252
    Mass (Da):29,423
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti550 – 5501I → L in CAA24295. (PubMed:7060132)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01106 Genomic RNA. Translation: CAA24295.1.
    AF389117 Genomic RNA. Translation: AAM75157.1.
    EF467820 Genomic RNA. Translation: ABO21708.1.
    CY009449 Genomic RNA. Translation: ABD77682.1.
    RefSeqiNP_040986.1. NC_002022.1.

    Genome annotation databases

    GeneIDi956535.

    Keywords - Coding sequence diversityi

    Ribosomal frameshifting

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01106 Genomic RNA. Translation: CAA24295.1 .
    AF389117 Genomic RNA. Translation: AAM75157.1 .
    EF467820 Genomic RNA. Translation: ABO21708.1 .
    CY009449 Genomic RNA. Translation: ABD77682.1 .
    RefSeqi NP_040986.1. NC_002022.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ZNL X-ray 2.30 A 239-716 [» ]
    ProteinModelPortali P03433.
    SMRi P03433. Positions 257-715.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-43996N.
    IntActi P03433. 35 interactions.
    MINTi MINT-3375052.

    Chemistry

    ChEMBLi CHEMBL1169598.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 956535.

    Enzyme and pathway databases

    Reactomei REACT_6145. Influenza Life Cycle.
    REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
    REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
    REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
    REACT_6212. Budding.
    REACT_6235. Packaging of Eight RNA Segments.
    REACT_6248. Transport of Ribonucleoproteins into the Host Nucleus.
    REACT_6321. Uncoating of the Influenza Virion.
    REACT_6326. Release.
    REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_9434. vRNP Assembly.
    REACT_9491. Viral mRNA Translation.
    REACT_9497. vRNA Synthesis.
    REACT_9527. cRNA Synthesis.

    Miscellaneous databases

    EvolutionaryTracei P03433.

    Family and domain databases

    InterProi IPR001009. RNA-dir_pol_influenzavirus.
    [Graphical view ]
    Pfami PF00603. Flu_PA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequences of influenza virus segments 1 and 3 reveal mosaic structure of a small viral RNA segment."
      Fields S., Winter G.
      Cell 28:303-313(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    3. "Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments."
      de Wit E., Spronken M.I.J., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D.M.E., Fouchier R.A.M.
      Virus Res. 103:155-161(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], REVERSE GENETICS.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    5. "The structural basis for an essential subunit interaction in influenza virus RNA polymerase."
      Obayashi E., Yoshida H., Kawai F., Shibayama N., Kawaguchi A., Nagata K., Tame J.R., Park S.Y.
      Nature 454:1127-1131(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 239-716.

    Entry informationi

    Entry nameiPA_I34A1
    AccessioniPrimary (citable) accession number: P03433
    Secondary accession number(s): A4GXH3, Q20N31, Q8JUU6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3