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P03431

- RDRP_I34A1

UniProt

P03431 - RDRP_I34A1

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Protein

RNA-directed RNA polymerase catalytic subunit

Gene

PB1

Organism
Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.1 Publication1 Publication

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: Reactome
  2. intracellular transport of virus Source: Reactome
  3. receptor-mediated endocytosis of virus by host cell Source: Reactome
  4. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
  5. transcription, DNA-templated Source: InterPro
  6. uncoating of virus Source: Reactome
  7. viral entry into host cell Source: Reactome
  8. viral genome maturation Source: Reactome
  9. viral genome packaging Source: Reactome
  10. viral life cycle Source: Reactome
  11. viral process Source: Reactome
  12. viral release from host cell Source: Reactome
  13. viral RNA genome replication Source: InterPro
  14. viral transcription Source: Reactome
  15. virion assembly Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, Viral RNA replication, Viral transcription

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_6145. Influenza Life Cycle.
REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
REACT_6212. Budding.
REACT_6235. Packaging of Eight RNA Segments.
REACT_6248. Transport of Ribonucleoproteins into the Host Nucleus.
REACT_6321. Uncoating of the Influenza Virion.
REACT_6326. Release.
REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
REACT_6354. Viral Messenger RNA Synthesis.
REACT_9434. vRNP Assembly.
REACT_9491. Viral mRNA Translation.
REACT_9497. vRNA Synthesis.
REACT_9527. cRNA Synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase catalytic subunit (EC:2.7.7.48)
Alternative name(s):
Polymerase basic protein 1
Short name:
PB1
RNA-directed RNA polymerase subunit P1
Gene namesi
Name:PB1
OrganismiInfluenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Taxonomic identifieri211044 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000009255: Genome

Subcellular locationi

Host nucleus 1 Publication. Host cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. endosome lumen Source: Reactome
  3. extracellular region Source: Reactome
  4. host cell nucleus Source: UniProtKB-KW
  5. nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757RNA-directed RNA polymerase catalytic subunitPRO_0000078763Add
BLAST

Post-translational modificationi

Phosphorylated by host PRKCA.1 Publication

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). Interacts (via C-terminus) with PB2 (via N-terminus); this interaction is essential for transcription initiation.2 Publications

Protein-protein interaction databases

DIPiDIP-56974N.
IntActiP03431. 32 interactions.

Structurei

Secondary structure

1
757
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 106Combined sources
Helixi687 – 69913Combined sources
Helixi714 – 73118Combined sources
Helixi737 – 75519Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZNLX-ray2.30B1-81[»]
2ZTTX-ray2.10A/C679-757[»]
3A1GX-ray1.70A/C678-757[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03431.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini286 – 483198RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni249 – 2568Promoter-binding siteBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi187 – 1959Nuclear localization signalBy similarity
Motifi203 – 21614Nuclear localization signalBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
[Graphical view]
PfamiPF00602. Flu_PB1. 1 hit.
[Graphical view]
PIRSFiPIRSF000827. RdRPol_OMV. 1 hit.
PROSITEiPS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03431-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS
60 70 80 90 100
EKGRWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP
110 120 130 140 150
GIFENSCIET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF
160 170 180 190 200
RSNGLTANES GRLIDFLKDV MESMKKEEMG ITTHFQRKRR VRDNMTKKMI
210 220 230 240 250
TQRTIGKKKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG
260 270 280 290 300
FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTELSF
310 320 330 340 350
TITGDNTKWN ENQNPRMFLA MITYMTRNQP EWFRNVLSIA PIMFSNKMAR
360 370 380 390 400
LGKGYMFESK SMKLRTQIPA EMLASIDLKY FNDSTRKKIE KIRPLLIEGT
410 420 430 440 450
ASLSPGMMMG MFNMLSTVLG VSILNLGQKR YTKTTYWWDG LQSSDDFALI
460 470 480 490 500
VNAPNHEGIQ AGVDRFYRTC KLLGINMSKK KSYINRTGTF EFTSFFYRYG
510 520 530 540 550
FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL
560 570 580 590 600
FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTRSKAGLL VSDGGPNLYN
610 620 630 640 650
IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVSHKEIESM NNAVMMPAHG
660 670 680 690 700
PAKNMEYDAV ATTHSWIPKR NRSILNTSQR GVLEDEQMYQ RCCNLFEKFF
710 720 730 740 750
PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF TEIMKICSTI

EELRRQK
Length:757
Mass (Da):86,576
Last modified:October 2, 2007 - v2
Checksum:iFB23E4976E06C18F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531G → A in AAA43581. (PubMed:6281731)Curated
Sequence conflicti175 – 1751K → N in ABO21706. 1 PublicationCurated
Sequence conflicti175 – 1751K → N in ABD77683. (PubMed:15163504)Curated
Sequence conflicti205 – 2051I → M in ABO21706. 1 PublicationCurated
Sequence conflicti205 – 2051I → M in ABD77683. (PubMed:15163504)Curated
Sequence conflicti208 – 2081K → R in AAA43581. (PubMed:6281731)Curated
Sequence conflicti300 – 3001F → L in AAA43581. (PubMed:6281731)Curated
Sequence conflicti394 – 3941P → S in ABO21706. 1 PublicationCurated
Sequence conflicti394 – 3941P → S in ABD77683. (PubMed:15163504)Curated
Sequence conflicti473 – 4731L → H in AAA43581. (PubMed:6281731)Curated
Sequence conflicti517 – 5171I → S in AAA43581. (PubMed:6281731)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02151 Genomic RNA. Translation: AAA43581.1.
AF389116 Genomic RNA. Translation: AAM75156.1.
CY009450 Genomic RNA. Translation: ABD77683.1.
EF467819 Genomic RNA. Translation: ABO21706.1.
PIRiA93418. P1IV34.
RefSeqiNP_040985.1. NC_002021.1.

Genome annotation databases

GeneIDi956534.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02151 Genomic RNA. Translation: AAA43581.1 .
AF389116 Genomic RNA. Translation: AAM75156.1 .
CY009450 Genomic RNA. Translation: ABD77683.1 .
EF467819 Genomic RNA. Translation: ABO21706.1 .
PIRi A93418. P1IV34.
RefSeqi NP_040985.1. NC_002021.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2ZNL X-ray 2.30 B 1-81 [» ]
2ZTT X-ray 2.10 A/C 679-757 [» ]
3A1G X-ray 1.70 A/C 678-757 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-56974N.
IntActi P03431. 32 interactions.

Chemistry

ChEMBLi CHEMBL3137263.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 956534.

Enzyme and pathway databases

Reactomei REACT_6145. Influenza Life Cycle.
REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
REACT_6212. Budding.
REACT_6235. Packaging of Eight RNA Segments.
REACT_6248. Transport of Ribonucleoproteins into the Host Nucleus.
REACT_6321. Uncoating of the Influenza Virion.
REACT_6326. Release.
REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
REACT_6354. Viral Messenger RNA Synthesis.
REACT_9434. vRNP Assembly.
REACT_9491. Viral mRNA Translation.
REACT_9497. vRNA Synthesis.
REACT_9527. cRNA Synthesis.

Miscellaneous databases

EvolutionaryTracei P03431.

Family and domain databases

InterProi IPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
[Graphical view ]
Pfami PF00602. Flu_PB1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000827. RdRPol_OMV. 1 hit.
PROSITEi PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of human influenza A/PR/8/34 segment 2."
    Winter G., Fields S.
    Nucleic Acids Res. 10:2135-2143(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments."
    de Wit E., Spronken M.I.J., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D.M.E., Fouchier R.A.M.
    Virus Res. 103:155-161(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], REVERSE GENETICS.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  5. "Influenza virus PB1 protein is the minimal and essential subunit of RNA polymerase."
    Kobayashi M., Toyoda T., Ishihama A.
    Arch. Virol. 141:525-539(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Influenza A virus proteins PB1 and NS1 are subject to functionally important phosphorylation by protein kinase C."
    Mahmoudian S., Auerochs S., Grone M., Marschall M.
    J. Gen. Virol. 90:1392-1397(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION BY HOST PRKCA.
    Strain: A/WSN/33.
  7. "Nuclear import and assembly of influenza A virus RNA polymerase studied in live cells by fluorescence cross-correlation spectroscopy."
    Huet S., Avilov S.V., Ferbitz L., Daigle N., Cusack S., Ellenberg J.
    J. Virol. 84:1254-1264(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: A/Victoria/3/75.
  8. "The RNA polymerase of influenza a virus: mechanisms of viral transcription and replication."
    Fodor E.
    Acta Virol. 57:113-122(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON FUNCTION.
  9. "The structural basis for an essential subunit interaction in influenza virus RNA polymerase."
    Obayashi E., Yoshida H., Kawai F., Shibayama N., Kawaguchi A., Nagata K., Tame J.R., Park S.Y.
    Nature 454:1127-1131(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 1-81, INTERACTION WITH PA.
  10. "Structural insight into the essential PB1-PB2 subunit contact of the influenza virus RNA polymerase."
    Sugiyama K., Obayashi E., Kawaguchi A., Suzuki Y., Tame J.R., Nagata K., Park S.Y.
    EMBO J. 28:1803-1811(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 678-757, INTERACTION WITH PB2.

Entry informationi

Entry nameiRDRP_I34A1
AccessioniPrimary (citable) accession number: P03431
Secondary accession number(s): A4GXH1, Q20N30, Q8JUU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 2, 2007
Last modified: November 26, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3