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P03431

- RDRP_I34A1

UniProt

P03431 - RDRP_I34A1

Protein

RNA-directed RNA polymerase catalytic subunit

Gene

PB1

Organism
Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 2 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    RNA-dependent RNA polymerase which is responsible for replication and transcription of virus segments. Binds the promoter sequence of the encapsidated viral RNA. Displays an endonuclease activity involved in cap-stealing. Cleaves cellular pre-mRNA to generate primers for viral transcription.

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. fusion of virus membrane with host plasma membrane Source: Reactome
    2. intracellular transport of virus Source: Reactome
    3. receptor-mediated endocytosis of virus by host cell Source: Reactome
    4. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
    5. transcription, DNA-templated Source: InterPro
    6. uncoating of virus Source: Reactome
    7. viral entry into host cell Source: Reactome
    8. viral genome maturation Source: Reactome
    9. viral genome packaging Source: Reactome
    10. viral life cycle Source: Reactome
    11. viral process Source: Reactome
    12. viral release from host cell Source: Reactome
    13. viral RNA genome replication Source: InterPro
    14. viral transcription Source: Reactome
    15. virion assembly Source: Reactome

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, Viral RNA replication

    Keywords - Ligandi

    Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_6145. Influenza Life Cycle.
    REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
    REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
    REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
    REACT_6212. Budding.
    REACT_6235. Packaging of Eight RNA Segments.
    REACT_6248. Transport of Ribonucleoproteins into the Host Nucleus.
    REACT_6321. Uncoating of the Influenza Virion.
    REACT_6326. Release.
    REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_9434. vRNP Assembly.
    REACT_9491. Viral mRNA Translation.
    REACT_9497. vRNA Synthesis.
    REACT_9527. cRNA Synthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase catalytic subunit (EC:2.7.7.48)
    Alternative name(s):
    Polymerase basic protein 1
    Short name:
    PB1
    RNA-directed RNA polymerase subunit P1
    Gene namesi
    Name:PB1
    OrganismiInfluenza A virus (strain A/Puerto Rico/8/1934 H1N1)
    Taxonomic identifieri211044 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Homo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000009255: Genome

    Subcellular locationi

    Host nucleus Curated

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. endosome lumen Source: Reactome
    3. extracellular region Source: Reactome
    4. host cell nucleus Source: UniProtKB-SubCell
    5. nucleoplasm Source: Reactome

    Keywords - Cellular componenti

    Host nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 757757RNA-directed RNA polymerase catalytic subunitPRO_0000078763Add
    BLAST

    Interactioni

    Subunit structurei

    Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

    Protein-protein interaction databases

    DIPiDIP-56974N.
    IntActiP03431. 32 interactions.

    Structurei

    Secondary structure

    1
    757
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 106
    Helixi687 – 69913
    Helixi714 – 73118
    Helixi737 – 75519

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ZNLX-ray2.30B1-81[»]
    2ZTTX-ray2.10A/C679-757[»]
    3A1GX-ray1.70A/C678-757[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03431.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini286 – 483198RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni249 – 2568Promoter-binding siteBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi187 – 1959Nuclear localization signalBy similarity
    Motifi203 – 21614Nuclear localization signalBy similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR007099. RNA-dir_pol_NSvirus.
    IPR001407. RNA_pol_PB1_influenza.
    [Graphical view]
    PfamiPF00602. Flu_PB1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000827. RdRPol_OMV. 1 hit.
    PROSITEiPS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P03431-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS    50
    EKGRWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP 100
    GIFENSCIET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF 150
    RSNGLTANES GRLIDFLKDV MESMKKEEMG ITTHFQRKRR VRDNMTKKMI 200
    TQRTIGKKKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG 250
    FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTELSF 300
    TITGDNTKWN ENQNPRMFLA MITYMTRNQP EWFRNVLSIA PIMFSNKMAR 350
    LGKGYMFESK SMKLRTQIPA EMLASIDLKY FNDSTRKKIE KIRPLLIEGT 400
    ASLSPGMMMG MFNMLSTVLG VSILNLGQKR YTKTTYWWDG LQSSDDFALI 450
    VNAPNHEGIQ AGVDRFYRTC KLLGINMSKK KSYINRTGTF EFTSFFYRYG 500
    FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL 550
    FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTRSKAGLL VSDGGPNLYN 600
    IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVSHKEIESM NNAVMMPAHG 650
    PAKNMEYDAV ATTHSWIPKR NRSILNTSQR GVLEDEQMYQ RCCNLFEKFF 700
    PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF TEIMKICSTI 750
    EELRRQK 757
    Length:757
    Mass (Da):86,576
    Last modified:October 2, 2007 - v2
    Checksum:iFB23E4976E06C18F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti53 – 531G → A in AAA43581. (PubMed:6281731)Curated
    Sequence conflicti175 – 1751K → N in ABO21706. 1 PublicationCurated
    Sequence conflicti175 – 1751K → N in ABD77683. (PubMed:15163504)Curated
    Sequence conflicti205 – 2051I → M in ABO21706. 1 PublicationCurated
    Sequence conflicti205 – 2051I → M in ABD77683. (PubMed:15163504)Curated
    Sequence conflicti208 – 2081K → R in AAA43581. (PubMed:6281731)Curated
    Sequence conflicti300 – 3001F → L in AAA43581. (PubMed:6281731)Curated
    Sequence conflicti394 – 3941P → S in ABO21706. 1 PublicationCurated
    Sequence conflicti394 – 3941P → S in ABD77683. (PubMed:15163504)Curated
    Sequence conflicti473 – 4731L → H in AAA43581. (PubMed:6281731)Curated
    Sequence conflicti517 – 5171I → S in AAA43581. (PubMed:6281731)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02151 Genomic RNA. Translation: AAA43581.1.
    AF389116 Genomic RNA. Translation: AAM75156.1.
    CY009450 Genomic RNA. Translation: ABD77683.1.
    EF467819 Genomic RNA. Translation: ABO21706.1.
    PIRiA93418. P1IV34.
    RefSeqiNP_040985.1. NC_002021.1.

    Genome annotation databases

    GeneIDi956534.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02151 Genomic RNA. Translation: AAA43581.1 .
    AF389116 Genomic RNA. Translation: AAM75156.1 .
    CY009450 Genomic RNA. Translation: ABD77683.1 .
    EF467819 Genomic RNA. Translation: ABO21706.1 .
    PIRi A93418. P1IV34.
    RefSeqi NP_040985.1. NC_002021.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ZNL X-ray 2.30 B 1-81 [» ]
    2ZTT X-ray 2.10 A/C 679-757 [» ]
    3A1G X-ray 1.70 A/C 678-757 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-56974N.
    IntActi P03431. 32 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 956534.

    Enzyme and pathway databases

    Reactomei REACT_6145. Influenza Life Cycle.
    REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
    REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
    REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
    REACT_6212. Budding.
    REACT_6235. Packaging of Eight RNA Segments.
    REACT_6248. Transport of Ribonucleoproteins into the Host Nucleus.
    REACT_6321. Uncoating of the Influenza Virion.
    REACT_6326. Release.
    REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_9434. vRNP Assembly.
    REACT_9491. Viral mRNA Translation.
    REACT_9497. vRNA Synthesis.
    REACT_9527. cRNA Synthesis.

    Miscellaneous databases

    EvolutionaryTracei P03431.

    Family and domain databases

    InterProi IPR007099. RNA-dir_pol_NSvirus.
    IPR001407. RNA_pol_PB1_influenza.
    [Graphical view ]
    Pfami PF00602. Flu_PB1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000827. RdRPol_OMV. 1 hit.
    PROSITEi PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of human influenza A/PR/8/34 segment 2."
      Winter G., Fields S.
      Nucleic Acids Res. 10:2135-2143(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    3. "Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments."
      de Wit E., Spronken M.I.J., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D.M.E., Fouchier R.A.M.
      Virus Res. 103:155-161(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], REVERSE GENETICS.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiRDRP_I34A1
    AccessioniPrimary (citable) accession number: P03431
    Secondary accession number(s): A4GXH1, Q20N30, Q8JUU7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 92 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3