P03431 (RDRP_I34A1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase catalytic subunit
EC=2.7.7.48

Alternative name(s):
Polymerase basic protein 1
Short name=PB1
RNA-directed RNA polymerase subunit P1

Gene names

Name:

PB1

Organism

Influenza A virus (strain A/Puerto Rico/8/1934 H1N1) [Reference proteome]

Taxonomic identifier

211044 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A › Influenza A virus › H1N1 subtype

Virus host

Aves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	757 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of virus segments. Binds the promoter sequence of the encapsidated viral RNA. Displays an endonuclease activity involved in cap-stealing. Cleaves cellular pre-mRNA to generate primers for viral transcription.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.
Subcellular location	Host nucleus Potential.
Sequence similarities	Belongs to the influenza viruses polymerase PB1 family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Host gene expression shutoff by virus Host transcription shutoff by virus Host-virus interaction Inhibition of host RNA polymerase II by virus Viral RNA replication
Cellular component	Host nucleus
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	suppression by virus of host RNA polymerase II activity Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro uncoating of virus Traceable author statement. Source: Reactome viral entry into host cell via membrane fusion with the plasma membrane Traceable author statement. Source: Reactome viral entry into host cell via receptor-mediated endocytosis Traceable author statement. Source: Reactome viral genome maturation Traceable author statement. Source: Reactome viral genome packaging Traceable author statement. Source: Reactome viral genome replication Inferred from electronic annotation. Source: InterPro viral release from host cell Traceable author statement. Source: Reactome viral transcription Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome endosome lumen Traceable author statement. Source: Reactome extracellular region Traceable author statement. Source: Reactome host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell nucleoplasm Traceable author statement. Source: Reactome
Molecular_function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 757

757

RNA-directed RNA polymerase catalytic subunit

PRO_0000078763

Regions

Domain

286 – 483

198

RdRp catalytic

Region

249 – 256

8

Promoter-binding site By similarity

Motif

187 – 195

9

Nuclear localization signal By similarity

Motif

203 – 216

14

Nuclear localization signal By similarity

Experimental info

Sequence conflict

53

1

G → A in AAA43581. Ref.1

Sequence conflict

175

1

K → N in ABO21706. Ref.4

Sequence conflict

175

1

K → N in ABD77683. Ref.3

Sequence conflict

205

1

I → M in ABO21706. Ref.4

Sequence conflict

205

1

I → M in ABD77683. Ref.3

Sequence conflict

208

1

K → R in AAA43581. Ref.1

Sequence conflict

300

1

F → L in AAA43581. Ref.1

Sequence conflict

394

1

P → S in ABO21706. Ref.4

Sequence conflict

394

1

P → S in ABD77683. Ref.3

Sequence conflict

473

1

L → H in AAA43581. Ref.1

Sequence conflict

517

1

I → S in AAA43581. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

757

Helix Strand Turn

Details...

Helix

5 – 10

6

Helix

687 – 699

13

Helix

714 – 731

18

Helix

737 – 755

19

Sequences

	Sequence		Length	Mass (Da)
	P03431 [UniParc]. Last modified October 2, 2007. Version 2. Checksum: FB23E4976E06C18F	FASTA	757	86,576
	10 20 30 40 50 60 MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS EKGRWTTNTE 70 80 90 100 110 120 TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP GIFENSCIET MEVVQQTRVD 130 140 150 160 170 180 KLTQGRQTYD WTLNRNQPAA TALANTIEVF RSNGLTANES GRLIDFLKDV MESMKKEEMG 190 200 210 220 230 240 ITTHFQRKRR VRDNMTKKMI TQRTIGKKKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA 250 260 270 280 290 300 IATPGMQIRG FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTELSF 310 320 330 340 350 360 TITGDNTKWN ENQNPRMFLA MITYMTRNQP EWFRNVLSIA PIMFSNKMAR LGKGYMFESK 370 380 390 400 410 420 SMKLRTQIPA EMLASIDLKY FNDSTRKKIE KIRPLLIEGT ASLSPGMMMG MFNMLSTVLG 430 440 450 460 470 480 VSILNLGQKR YTKTTYWWDG LQSSDDFALI VNAPNHEGIQ AGVDRFYRTC KLLGINMSKK 490 500 510 520 530 540 KSYINRTGTF EFTSFFYRYG FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG 550 560 570 580 590 600 PATAQMALQL FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTRSKAGLL VSDGGPNLYN 610 620 630 640 650 660 IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVSHKEIESM NNAVMMPAHG PAKNMEYDAV 670 680 690 700 710 720 ATTHSWIPKR NRSILNTSQR GVLEDEQMYQ RCCNLFEKFF PSSSYRRPVG ISSMVEAMVS 730 740 750 RARIDARIDF ESGRIKKEEF TEIMKICSTI EELRRQK

References

[1]	"Nucleotide sequence of human influenza A/PR/8/34 segment 2." Winter G., Fields S. Nucleic Acids Res. 10:2135-2143(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Plasmid-only rescue of influenza A virus vaccine candidates." Schickli J.H., Flandorfer A., Nakaya T., Martinez-Sobrido L., Garcia-Sastre A., Palese P. Philos. Trans. R. Soc. Lond., B, Biol. Sci. 356:1965-1973(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments." de Wit E., Spronken M.I.J., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D.M.E., Fouchier R.A.M. Virus Res. 103:155-161(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], REVERSE GENETICS.
[4]	"The NIAID influenza genome sequencing project." Ghedin E., Spiro D., Miller N., Zaborsky J., Feldblyum T., Subbu V., Shumway M., Sparenborg J., Groveman L., Halpin R., Sitz J., Koo H., Salzberg S.L., Webster R.G., Hoffmann E., Krauss S., Naeve C., Bao Y. , Bolotov P., Dernovoy D., Kiryutin B., Lipman D.J., Tatusova T. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J02151 Genomic RNA. Translation: AAA43581.1.
AF389116 Genomic RNA. Translation: AAM75156.1.
CY009450 Genomic RNA. Translation: ABD77683.1.
EF467819 Genomic RNA. Translation: ABO21706.1.

PIR

P1IV34. A93418.

RefSeq

NP_040985.1. NC_002021.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ZNL	X-ray	2.30	B	1-81	[»]
2ZTT	X-ray	2.10	A/C	679-757	[»]
3A1G	X-ray	1.70	A/C	678-757	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-56974N.

IntAct

P03431. 32 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

956534.

Enzyme and pathway databases

Reactome

REACT_116125. Disease.

Family and domain databases

InterPro

IPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
[Graphical view]

Pfam

PF00602. Flu_PB1. 1 hit.
[Graphical view]

PIRSF

PIRSF000827. RdRPol_OMV. 1 hit.

PROSITE

PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P03431.

Entry information

Entry name

RDRP_I34A1

Accession

Primary (citable) accession number: P03431
Secondary accession number(s): A4GXH1, Q20N30, Q8JUU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	October 2, 2007
Last modified:	April 3, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program