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P03431

- RDRP_I34A1

UniProt

P03431 - RDRP_I34A1

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Protein
RNA-directed RNA polymerase catalytic subunit
Gene
PB1
Organism
Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus segments. Binds the promoter sequence of the encapsidated viral RNA. Displays an endonuclease activity involved in cap-stealing. Cleaves cellular pre-mRNA to generate primers for viral transcription.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

GO - Molecular functioni

  1. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  2. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: Reactome
  2. intracellular transport of virus Source: Reactome
  3. receptor-mediated endocytosis of virus by host cell Source: Reactome
  4. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
  5. transcription, DNA-templated Source: InterPro
  6. uncoating of virus Source: Reactome
  7. viral RNA genome replication Source: InterPro
  8. viral entry into host cell Source: Reactome
  9. viral genome maturation Source: Reactome
  10. viral genome packaging Source: Reactome
  11. viral life cycle Source: Reactome
  12. viral process Source: Reactome
  13. viral release from host cell Source: Reactome
  14. viral transcription Source: Reactome
  15. virion assembly Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, Viral RNA replication

Keywords - Ligandi

Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_6145. Influenza Life Cycle.
REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
REACT_6212. Budding.
REACT_6235. Packaging of Eight RNA Segments.
REACT_6248. Transport of Ribonucleoproteins into the Host Nucleus.
REACT_6321. Uncoating of the Influenza Virion.
REACT_6326. Release.
REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
REACT_6354. Viral Messenger RNA Synthesis.
REACT_9434. vRNP Assembly.
REACT_9491. Viral mRNA Translation.
REACT_9497. vRNA Synthesis.
REACT_9527. cRNA Synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase catalytic subunit (EC:2.7.7.48)
Alternative name(s):
Polymerase basic protein 1
Short name:
PB1
RNA-directed RNA polymerase subunit P1
Gene namesi
Name:PB1
OrganismiInfluenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Taxonomic identifieri211044 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000009255: Genome

Subcellular locationi

Host nucleus Reviewed prediction

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. endosome lumen Source: Reactome
  3. extracellular region Source: Reactome
  4. host cell nucleus Source: UniProtKB-SubCell
  5. nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757RNA-directed RNA polymerase catalytic subunit
PRO_0000078763Add
BLAST

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

Protein-protein interaction databases

DIPiDIP-56974N.
IntActiP03431. 32 interactions.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 106
Helixi687 – 69913
Helixi714 – 73118
Helixi737 – 75519

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZNLX-ray2.30B1-81[»]
2ZTTX-ray2.10A/C679-757[»]
3A1GX-ray1.70A/C678-757[»]

Miscellaneous databases

EvolutionaryTraceiP03431.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini286 – 483198RdRp catalytic
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni249 – 2568Promoter-binding site By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi187 – 1959Nuclear localization signal By similarity
Motifi203 – 21614Nuclear localization signal By similarity
Add
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
[Graphical view]
PfamiPF00602. Flu_PB1. 1 hit.
[Graphical view]
PIRSFiPIRSF000827. RdRPol_OMV. 1 hit.
PROSITEiPS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03431-1 [UniParc]FASTAAdd to Basket

« Hide

MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS    50
EKGRWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP 100
GIFENSCIET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF 150
RSNGLTANES GRLIDFLKDV MESMKKEEMG ITTHFQRKRR VRDNMTKKMI 200
TQRTIGKKKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG 250
FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTELSF 300
TITGDNTKWN ENQNPRMFLA MITYMTRNQP EWFRNVLSIA PIMFSNKMAR 350
LGKGYMFESK SMKLRTQIPA EMLASIDLKY FNDSTRKKIE KIRPLLIEGT 400
ASLSPGMMMG MFNMLSTVLG VSILNLGQKR YTKTTYWWDG LQSSDDFALI 450
VNAPNHEGIQ AGVDRFYRTC KLLGINMSKK KSYINRTGTF EFTSFFYRYG 500
FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL 550
FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTRSKAGLL VSDGGPNLYN 600
IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVSHKEIESM NNAVMMPAHG 650
PAKNMEYDAV ATTHSWIPKR NRSILNTSQR GVLEDEQMYQ RCCNLFEKFF 700
PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF TEIMKICSTI 750
EELRRQK 757
Length:757
Mass (Da):86,576
Last modified:October 2, 2007 - v2
Checksum:iFB23E4976E06C18F
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531G → A in AAA43581. 1 Publication
Sequence conflicti175 – 1751K → N in ABO21706. 1 Publication
Sequence conflicti175 – 1751K → N in ABD77683. 1 Publication
Sequence conflicti205 – 2051I → M in ABO21706. 1 Publication
Sequence conflicti205 – 2051I → M in ABD77683. 1 Publication
Sequence conflicti208 – 2081K → R in AAA43581. 1 Publication
Sequence conflicti300 – 3001F → L in AAA43581. 1 Publication
Sequence conflicti394 – 3941P → S in ABO21706. 1 Publication
Sequence conflicti394 – 3941P → S in ABD77683. 1 Publication
Sequence conflicti473 – 4731L → H in AAA43581. 1 Publication
Sequence conflicti517 – 5171I → S in AAA43581. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02151 Genomic RNA. Translation: AAA43581.1.
AF389116 Genomic RNA. Translation: AAM75156.1.
CY009450 Genomic RNA. Translation: ABD77683.1.
EF467819 Genomic RNA. Translation: ABO21706.1.
PIRiA93418. P1IV34.
RefSeqiNP_040985.1. NC_002021.1.

Genome annotation databases

GeneIDi956534.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02151 Genomic RNA. Translation: AAA43581.1 .
AF389116 Genomic RNA. Translation: AAM75156.1 .
CY009450 Genomic RNA. Translation: ABD77683.1 .
EF467819 Genomic RNA. Translation: ABO21706.1 .
PIRi A93418. P1IV34.
RefSeqi NP_040985.1. NC_002021.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2ZNL X-ray 2.30 B 1-81 [» ]
2ZTT X-ray 2.10 A/C 679-757 [» ]
3A1G X-ray 1.70 A/C 678-757 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-56974N.
IntActi P03431. 32 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 956534.

Enzyme and pathway databases

Reactomei REACT_6145. Influenza Life Cycle.
REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
REACT_6212. Budding.
REACT_6235. Packaging of Eight RNA Segments.
REACT_6248. Transport of Ribonucleoproteins into the Host Nucleus.
REACT_6321. Uncoating of the Influenza Virion.
REACT_6326. Release.
REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
REACT_6354. Viral Messenger RNA Synthesis.
REACT_9434. vRNP Assembly.
REACT_9491. Viral mRNA Translation.
REACT_9497. vRNA Synthesis.
REACT_9527. cRNA Synthesis.

Miscellaneous databases

EvolutionaryTracei P03431.

Family and domain databases

InterProi IPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
[Graphical view ]
Pfami PF00602. Flu_PB1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000827. RdRPol_OMV. 1 hit.
PROSITEi PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of human influenza A/PR/8/34 segment 2."
    Winter G., Fields S.
    Nucleic Acids Res. 10:2135-2143(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments."
    de Wit E., Spronken M.I.J., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D.M.E., Fouchier R.A.M.
    Virus Res. 103:155-161(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], REVERSE GENETICS.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiRDRP_I34A1
AccessioniPrimary (citable) accession number: P03431
Secondary accession number(s): A4GXH1, Q20N30, Q8JUU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 2, 2007
Last modified: September 3, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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