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P03430

- RDRP_I33A0

UniProt

P03430 - RDRP_I33A0

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Protein
RNA-directed RNA polymerase catalytic subunit
Gene
PB1
Organism
Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus segments. Binds the promoter sequence of the encapsidated viral RNA. Displays an endonuclease activity involved in cap-stealing. Cleaves cellular pre-mRNA to generate primers for viral transcription.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

GO - Molecular functioni

  1. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  2. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
  2. transcription, DNA-templated Source: InterPro
  3. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, Viral RNA replication

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase catalytic subunit (EC:2.7.7.48)
Alternative name(s):
Polymerase basic protein 1
Short name:
PB1
RNA-directed RNA polymerase subunit P1
Gene namesi
Name:PB1
OrganismiInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Taxonomic identifieri381518 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000834: Genome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi233 – 2331R → A: Complete loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi235 – 2351K → A: Complete loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi238 – 2381R → A: Complete loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi239 – 2391R → A: Complete loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi249 – 2491R → A: Complete loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi251 – 2511F → A: 20% loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi254 – 2541F → A: 50% loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi269 – 2691S → A: 70% loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi273 – 2731V → A: 80% loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi278 – 2781K → A: Complete loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi281 – 2811K → A: Complete loss of RNA synthesis in vitro. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757RNA-directed RNA polymerase catalytic subunit
PRO_0000078767Add
BLAST

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

Protein-protein interaction databases

IntActiP03430. 1 interaction.
MINTiMINT-8081772.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini286 – 483198RdRp catalytic
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni249 – 2568Promoter-binding site Inferred

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi187 – 1959Nuclear localization signal1 Publication
Motifi203 – 21614Nuclear localization signal1 Publication
Add
BLAST

Sequence similaritiesi

Family and domain databases

InterProiIPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
[Graphical view]
PfamiPF00602. Flu_PB1. 1 hit.
[Graphical view]
PIRSFiPIRSF000827. RdRPol_OMV. 1 hit.
PROSITEiPS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03430-1 [UniParc]FASTAAdd to Basket

« Hide

MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS    50
ERGRWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP 100
GIFETSCLET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF 150
RSNGLTANES GRLIDFLKDV MESMNKEEME ITTHFQRKRR VRDNMTKKMV 200
TQRTIGKRKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG 250
FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF 300
TITGDNTKWN ENQNPRMFLA MITYITRNQP EWFRNVLSIA PIMFSNKMAR 350
LGKGYMFESK SMKIRTQIPA EMLASIDLKY FNDSTRKKIE KIRPLLIDGT 400
ASLSPGMMMG MFNMLSTVLG VSILNLGQKR HTKTTYWWDG LQSSDDFALI 450
VNAPNHEGIQ AGVNRFYRTC KLLGINMSKK KSYINRTGTF EFTSFFYRYG 500
FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL 550
FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTHSKAGLL VSDGGPNLYN 600
IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVNHKDIESV NNAVIMPAHG 650
PAKNMEYDAV ATTHSWIPKR NRSILNTSQR GILEDEQMYQ KCCNLFEKFF 700
PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF TEIMKICSTI 750
EELRRQK 757
Length:757
Mass (Da):86,534
Last modified:July 21, 1986 - v1
Checksum:iD5A0E8133CDE37F4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02178 Genomic RNA. Translation: AAA43582.1. Sequence problems.
PIRiA04046. P1IV33.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02178 Genomic RNA. Translation: AAA43582.1 . Sequence problems.
PIRi A04046. P1IV33.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P03430. 1 interaction.
MINTi MINT-8081772.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
[Graphical view ]
Pfami PF00602. Flu_PB1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000827. RdRPol_OMV. 1 hit.
PROSITEi PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the polymerase 1 gene and the secondary structure prediction of polymerase 1 protein of human influenza virus A/WSN/33."
    Sivasubramanian N., Nayak D.P.
    J. Virol. 44:321-329(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Function of two discrete regions is required for nuclear localization of polymerase basic protein 1 of A/WSN/33 influenza virus (H1 N1)."
    Nath S.T., Nayak D.P.
    Mol. Cell. Biol. 10:4139-4145(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEAR LOCALIZATION SIGNAL.
  3. "A new promoter-binding site in the PB1 subunit of the influenza A virus polymerase."
    Jung T.E., Brownlee G.G.
    J. Gen. Virol. 87:679-688(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ARG-233; LYS-235; ARG-238; ARG-239; ARG-249; PHE-251; PHE-254; SER-269; VAL-273; LYS-278 AND LYS-281.

Entry informationi

Entry nameiRDRP_I33A0
AccessioniPrimary (citable) accession number: P03430
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 9, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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