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P03430 (RDRP_I33A0) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RNA-directed RNA polymerase catalytic subunit

EC=2.7.7.48
Alternative name(s):
Polymerase basic protein 1
Short name=PB1
RNA-directed RNA polymerase subunit P1
Gene names
Name:PB1
OrganismInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1)) [Complete proteome]
Taxonomic identifier381518 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length757 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus segments. Binds the promoter sequence of the encapsidated viral RNA. Displays an endonuclease activity involved in cap-stealing. Cleaves cellular pre-mRNA to generate primers for viral transcription.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subunit structure

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

Sequence similarities

Belongs to the influenza viruses polymerase PB1 family.

Contains 1 RdRp catalytic domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 757757RNA-directed RNA polymerase catalytic subunit
PRO_0000078767

Regions

Domain286 – 483198RdRp catalytic
Region249 – 2568Promoter-binding site Probable
Motif187 – 1959Nuclear localization signal Ref.2
Motif203 – 21614Nuclear localization signal Ref.2

Experimental info

Mutagenesis2331R → A: Complete loss of RNA synthesis in vitro. Ref.3
Mutagenesis2351K → A: Complete loss of RNA synthesis in vitro. Ref.3
Mutagenesis2381R → A: Complete loss of RNA synthesis in vitro. Ref.3
Mutagenesis2391R → A: Complete loss of RNA synthesis in vitro. Ref.3
Mutagenesis2491R → A: Complete loss of RNA synthesis in vitro. Ref.3
Mutagenesis2511F → A: 20% loss of RNA synthesis in vitro. Ref.3
Mutagenesis2541F → A: 50% loss of RNA synthesis in vitro. Ref.3
Mutagenesis2691S → A: 70% loss of RNA synthesis in vitro. Ref.3
Mutagenesis2731V → A: 80% loss of RNA synthesis in vitro. Ref.3
Mutagenesis2781K → A: Complete loss of RNA synthesis in vitro. Ref.3
Mutagenesis2811K → A: Complete loss of RNA synthesis in vitro. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P03430 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: D5A0E8133CDE37F4

FASTA75786,534
        10         20         30         40         50         60 
MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS ERGRWTTNTE 

        70         80         90        100        110        120 
TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP GIFETSCLET MEVVQQTRVD 

       130        140        150        160        170        180 
KLTQGRQTYD WTLNRNQPAA TALANTIEVF RSNGLTANES GRLIDFLKDV MESMNKEEME 

       190        200        210        220        230        240 
ITTHFQRKRR VRDNMTKKMV TQRTIGKRKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA 

       250        260        270        280        290        300 
IATPGMQIRG FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF 

       310        320        330        340        350        360 
TITGDNTKWN ENQNPRMFLA MITYITRNQP EWFRNVLSIA PIMFSNKMAR LGKGYMFESK 

       370        380        390        400        410        420 
SMKIRTQIPA EMLASIDLKY FNDSTRKKIE KIRPLLIDGT ASLSPGMMMG MFNMLSTVLG 

       430        440        450        460        470        480 
VSILNLGQKR HTKTTYWWDG LQSSDDFALI VNAPNHEGIQ AGVNRFYRTC KLLGINMSKK 

       490        500        510        520        530        540 
KSYINRTGTF EFTSFFYRYG FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG 

       550        560        570        580        590        600 
PATAQMALQL FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTHSKAGLL VSDGGPNLYN 

       610        620        630        640        650        660 
IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVNHKDIESV NNAVIMPAHG PAKNMEYDAV 

       670        680        690        700        710        720 
ATTHSWIPKR NRSILNTSQR GILEDEQMYQ KCCNLFEKFF PSSSYRRPVG ISSMVEAMVS 

       730        740        750 
RARIDARIDF ESGRIKKEEF TEIMKICSTI EELRRQK 

« Hide

References

[1]"Sequence analysis of the polymerase 1 gene and the secondary structure prediction of polymerase 1 protein of human influenza virus A/WSN/33."
Sivasubramanian N., Nayak D.P.
J. Virol. 44:321-329(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Function of two discrete regions is required for nuclear localization of polymerase basic protein 1 of A/WSN/33 influenza virus (H1 N1)."
Nath S.T., Nayak D.P.
Mol. Cell. Biol. 10:4139-4145(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEAR LOCALIZATION SIGNAL.
[3]"A new promoter-binding site in the PB1 subunit of the influenza A virus polymerase."
Jung T.E., Brownlee G.G.
J. Gen. Virol. 87:679-688(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF ARG-233; LYS-235; ARG-238; ARG-239; ARG-249; PHE-251; PHE-254; SER-269; VAL-273; LYS-278 AND LYS-281.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02178 Genomic RNA. Translation: AAA43582.1. Sequence problems.
PIRP1IV33. A04046.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP03430. 1 interaction.
MINTMINT-8081772.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
[Graphical view]
PfamPF00602. Flu_PB1. 1 hit.
[Graphical view]
PIRSFPIRSF000827. RdRPol_OMV. 1 hit.
PROSITEPS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRDRP_I33A0
AccessionPrimary (citable) accession number: P03430
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families