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P03430

- RDRP_I33A0

UniProt

P03430 - RDRP_I33A0

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Protein

RNA-directed RNA polymerase catalytic subunit

Gene

PB1

Organism
Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

RNA-dependent RNA polymerase which is responsible for replication and transcription of virus RNA segments. The transcription of viral mRNAs occurs by a unique mechanism called cap-snatching. 5' methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides by PA. In turn, these short capped RNAs are used as primers by PB1 for transcription of viral mRNAs. During virus replication, PB1 initiates RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn serves as a template for the production of more vRNAs.By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

GO - Molecular functioni

  1. nucleotide binding Source: UniProtKB-KW
  2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

GO - Biological processi

  1. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
  2. transcription, DNA-templated Source: InterPro
  3. viral RNA genome replication Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

Keywords - Biological processi

Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, Viral RNA replication, Viral transcription

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-directed RNA polymerase catalytic subunit (EC:2.7.7.48)
Alternative name(s):
Polymerase basic protein 1
Short name:
PB1
RNA-directed RNA polymerase subunit P1
Gene namesi
Name:PB1
OrganismiInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Taxonomic identifieri381518 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000834: Genome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi233 – 2331R → A: Complete loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi235 – 2351K → A: Complete loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi238 – 2381R → A: Complete loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi239 – 2391R → A: Complete loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi249 – 2491R → A: Complete loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi251 – 2511F → A: 20% loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi254 – 2541F → A: 50% loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi269 – 2691S → A: 70% loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi273 – 2731V → A: 80% loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi278 – 2781K → A: Complete loss of RNA synthesis in vitro. 1 Publication
Mutagenesisi281 – 2811K → A: Complete loss of RNA synthesis in vitro. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757RNA-directed RNA polymerase catalytic subunitPRO_0000078767Add
BLAST

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PA (via C-terminus). Interacts (via C-terminus) with PB2 (via N-terminus); this interaction is essential for transcription initiation.By similarity

Protein-protein interaction databases

IntActiP03430. 1 interaction.
MINTiMINT-8081772.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini286 – 483198RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni249 – 2568Promoter-binding siteCurated

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi187 – 1959Nuclear localization signal1 Publication
Motifi203 – 21614Nuclear localization signal1 PublicationAdd
BLAST

Sequence similaritiesi

Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
[Graphical view]
PfamiPF00602. Flu_PB1. 1 hit.
[Graphical view]
PIRSFiPIRSF000827. RdRPol_OMV. 1 hit.
PROSITEiPS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03430-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS
60 70 80 90 100
ERGRWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP
110 120 130 140 150
GIFETSCLET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF
160 170 180 190 200
RSNGLTANES GRLIDFLKDV MESMNKEEME ITTHFQRKRR VRDNMTKKMV
210 220 230 240 250
TQRTIGKRKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG
260 270 280 290 300
FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF
310 320 330 340 350
TITGDNTKWN ENQNPRMFLA MITYITRNQP EWFRNVLSIA PIMFSNKMAR
360 370 380 390 400
LGKGYMFESK SMKIRTQIPA EMLASIDLKY FNDSTRKKIE KIRPLLIDGT
410 420 430 440 450
ASLSPGMMMG MFNMLSTVLG VSILNLGQKR HTKTTYWWDG LQSSDDFALI
460 470 480 490 500
VNAPNHEGIQ AGVNRFYRTC KLLGINMSKK KSYINRTGTF EFTSFFYRYG
510 520 530 540 550
FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL
560 570 580 590 600
FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTHSKAGLL VSDGGPNLYN
610 620 630 640 650
IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVNHKDIESV NNAVIMPAHG
660 670 680 690 700
PAKNMEYDAV ATTHSWIPKR NRSILNTSQR GILEDEQMYQ KCCNLFEKFF
710 720 730 740 750
PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF TEIMKICSTI

EELRRQK
Length:757
Mass (Da):86,534
Last modified:July 21, 1986 - v1
Checksum:iD5A0E8133CDE37F4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02178 Genomic RNA. Translation: AAA43582.1. Sequence problems.
PIRiA04046. P1IV33.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02178 Genomic RNA. Translation: AAA43582.1 . Sequence problems.
PIRi A04046. P1IV33.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P03430. 1 interaction.
MINTi MINT-8081772.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR007099. RNA-dir_pol_NSvirus.
IPR001407. RNA_pol_PB1_influenza.
[Graphical view ]
Pfami PF00602. Flu_PB1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000827. RdRPol_OMV. 1 hit.
PROSITEi PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence analysis of the polymerase 1 gene and the secondary structure prediction of polymerase 1 protein of human influenza virus A/WSN/33."
    Sivasubramanian N., Nayak D.P.
    J. Virol. 44:321-329(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Function of two discrete regions is required for nuclear localization of polymerase basic protein 1 of A/WSN/33 influenza virus (H1 N1)."
    Nath S.T., Nayak D.P.
    Mol. Cell. Biol. 10:4139-4145(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEAR LOCALIZATION SIGNAL.
  3. "A new promoter-binding site in the PB1 subunit of the influenza A virus polymerase."
    Jung T.E., Brownlee G.G.
    J. Gen. Virol. 87:679-688(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ARG-233; LYS-235; ARG-238; ARG-239; ARG-249; PHE-251; PHE-254; SER-269; VAL-273; LYS-278 AND LYS-281.

Entry informationi

Entry nameiRDRP_I33A0
AccessioniPrimary (citable) accession number: P03430
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 29, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3