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P03430

- RDRP_I33A0

UniProt

P03430 - RDRP_I33A0

Protein

RNA-directed RNA polymerase catalytic subunit

Gene

PB1

Organism
Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    RNA-dependent RNA polymerase which is responsible for replication and transcription of virus segments. Binds the promoter sequence of the encapsidated viral RNA. Displays an endonuclease activity involved in cap-stealing. Cleaves cellular pre-mRNA to generate primers for viral transcription.

    Catalytic activityi

    Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation

    GO - Molecular functioni

    1. nucleotide binding Source: UniProtKB-KW
    2. RNA-directed RNA polymerase activity Source: UniProtKB-KW

    GO - Biological processi

    1. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
    2. transcription, DNA-templated Source: InterPro
    3. viral RNA genome replication Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, RNA-directed RNA polymerase, Transferase

    Keywords - Biological processi

    Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, Viral RNA replication

    Keywords - Ligandi

    Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA-directed RNA polymerase catalytic subunit (EC:2.7.7.48)
    Alternative name(s):
    Polymerase basic protein 1
    Short name:
    PB1
    RNA-directed RNA polymerase subunit P1
    Gene namesi
    Name:PB1
    OrganismiInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
    Taxonomic identifieri381518 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Homo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000000834: Genome

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi233 – 2331R → A: Complete loss of RNA synthesis in vitro. 1 Publication
    Mutagenesisi235 – 2351K → A: Complete loss of RNA synthesis in vitro. 1 Publication
    Mutagenesisi238 – 2381R → A: Complete loss of RNA synthesis in vitro. 1 Publication
    Mutagenesisi239 – 2391R → A: Complete loss of RNA synthesis in vitro. 1 Publication
    Mutagenesisi249 – 2491R → A: Complete loss of RNA synthesis in vitro. 1 Publication
    Mutagenesisi251 – 2511F → A: 20% loss of RNA synthesis in vitro. 1 Publication
    Mutagenesisi254 – 2541F → A: 50% loss of RNA synthesis in vitro. 1 Publication
    Mutagenesisi269 – 2691S → A: 70% loss of RNA synthesis in vitro. 1 Publication
    Mutagenesisi273 – 2731V → A: 80% loss of RNA synthesis in vitro. 1 Publication
    Mutagenesisi278 – 2781K → A: Complete loss of RNA synthesis in vitro. 1 Publication
    Mutagenesisi281 – 2811K → A: Complete loss of RNA synthesis in vitro. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 757757RNA-directed RNA polymerase catalytic subunitPRO_0000078767Add
    BLAST

    Interactioni

    Subunit structurei

    Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

    Protein-protein interaction databases

    IntActiP03430. 1 interaction.
    MINTiMINT-8081772.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini286 – 483198RdRp catalyticPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni249 – 2568Promoter-binding siteCurated

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi187 – 1959Nuclear localization signal1 Publication
    Motifi203 – 21614Nuclear localization signal1 PublicationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR007099. RNA-dir_pol_NSvirus.
    IPR001407. RNA_pol_PB1_influenza.
    [Graphical view]
    PfamiPF00602. Flu_PB1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000827. RdRPol_OMV. 1 hit.
    PROSITEiPS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P03430-1 [UniParc]FASTAAdd to Basket

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    MDVNPTLLFL KVPAQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS    50
    ERGRWTTNTE TGAPQLNPID GPLPEDNEPS GYAQTDCVLE AMAFLEESHP 100
    GIFETSCLET MEVVQQTRVD KLTQGRQTYD WTLNRNQPAA TALANTIEVF 150
    RSNGLTANES GRLIDFLKDV MESMNKEEME ITTHFQRKRR VRDNMTKKMV 200
    TQRTIGKRKQ RLNKRSYLIR ALTLNTMTKD AERGKLKRRA IATPGMQIRG 250
    FVYFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTEISF 300
    TITGDNTKWN ENQNPRMFLA MITYITRNQP EWFRNVLSIA PIMFSNKMAR 350
    LGKGYMFESK SMKIRTQIPA EMLASIDLKY FNDSTRKKIE KIRPLLIDGT 400
    ASLSPGMMMG MFNMLSTVLG VSILNLGQKR HTKTTYWWDG LQSSDDFALI 450
    VNAPNHEGIQ AGVNRFYRTC KLLGINMSKK KSYINRTGTF EFTSFFYRYG 500
    FVANFSMELP SFGVSGINES ADMSIGVTVI KNNMINNDLG PATAQMALQL 550
    FIKDYRYTYR CHRGDTQIQT RRSFEIKKLW EQTHSKAGLL VSDGGPNLYN 600
    IRNLHIPEVC LKWELMDEDY QGRLCNPLNP FVNHKDIESV NNAVIMPAHG 650
    PAKNMEYDAV ATTHSWIPKR NRSILNTSQR GILEDEQMYQ KCCNLFEKFF 700
    PSSSYRRPVG ISSMVEAMVS RARIDARIDF ESGRIKKEEF TEIMKICSTI 750
    EELRRQK 757
    Length:757
    Mass (Da):86,534
    Last modified:July 21, 1986 - v1
    Checksum:iD5A0E8133CDE37F4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02178 Genomic RNA. Translation: AAA43582.1. Sequence problems.
    PIRiA04046. P1IV33.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02178 Genomic RNA. Translation: AAA43582.1 . Sequence problems.
    PIRi A04046. P1IV33.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P03430. 1 interaction.
    MINTi MINT-8081772.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR007099. RNA-dir_pol_NSvirus.
    IPR001407. RNA_pol_PB1_influenza.
    [Graphical view ]
    Pfami PF00602. Flu_PB1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000827. RdRPol_OMV. 1 hit.
    PROSITEi PS50525. RDRP_SSRNA_NEG_SEG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the polymerase 1 gene and the secondary structure prediction of polymerase 1 protein of human influenza virus A/WSN/33."
      Sivasubramanian N., Nayak D.P.
      J. Virol. 44:321-329(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Function of two discrete regions is required for nuclear localization of polymerase basic protein 1 of A/WSN/33 influenza virus (H1 N1)."
      Nath S.T., Nayak D.P.
      Mol. Cell. Biol. 10:4139-4145(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEAR LOCALIZATION SIGNAL.
    3. "A new promoter-binding site in the PB1 subunit of the influenza A virus polymerase."
      Jung T.E., Brownlee G.G.
      J. Gen. Virol. 87:679-688(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ARG-233; LYS-235; ARG-238; ARG-239; ARG-249; PHE-251; PHE-254; SER-269; VAL-273; LYS-278 AND LYS-281.

    Entry informationi

    Entry nameiRDRP_I33A0
    AccessioniPrimary (citable) accession number: P03430
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3