Polymerase basic protein 2 - Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)

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P03428 (PB2_I34A1) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 85. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Polymerase basic protein 2

Alternative name(s):
RNA-directed RNA polymerase subunit P3

Gene names

Name:

PB2

Organism

Influenza A virus (strain A/Puerto Rico/8/1934 H1N1) [Reference proteome]

Taxonomic identifier

211044 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A ›

Virus host

Aves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication By similarity.
Subunit structure	Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.
Subcellular location	Virion. Host nucleus. Host mitochondrion By similarity.
Sequence similarities	Belongs to the influenza viruses PB2 family.

Ontologies

Keywords
Biological process	Cap snatching Host gene expression shutoff by virus Host transcription shutoff by virus Host-virus interaction Inhibition of host RNA polymerase II by virus mRNA capping mRNA processing
Cellular component	Host mitochondrion Host nucleus Virion
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	7-methylguanosine mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW suppression by virus of host RNA polymerase II activity Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro uncoating of virus Traceable author statement. Source: Reactome viral entry into host cell via membrane fusion with the plasma membrane Traceable author statement. Source: Reactome viral entry into host cell via receptor-mediated endocytosis Traceable author statement. Source: Reactome viral genome maturation Traceable author statement. Source: Reactome viral genome packaging Traceable author statement. Source: Reactome viral release from host cell Traceable author statement. Source: Reactome viral transcription Traceable author statement. Source: Reactome
Cellular_component	cytosol Traceable author statement. Source: Reactome endosome lumen Traceable author statement. Source: Reactome extracellular region Traceable author statement. Source: Reactome host cell mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell nucleoplasm Traceable author statement. Source: Reactome virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 759

759

Polymerase basic protein 2

PRO_0000078834

Regions

Motif

736 – 739

Nuclear localization signal By similarity

Experimental info

Sequence conflict

105

I → M in ABD77685. Ref.1

Sequence conflict

251

R → K in ABD77685. Ref.1

Sequence conflict

299

R → K in ABD77685. Ref.1

Sequence conflict

309

D → G in ABD77685. Ref.1

Sequence conflict

504

I → V in ABD77685. Ref.1

Sequence conflict

701

D → N in ABO21705. Ref.3

Sequence conflict

702

K → R in ABD77685. Ref.1

Secondary structure

759

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P03428 [UniParc].

Last modified March 6, 2007. Version 2.
Checksum: 10D2D1608AE536D2
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FASTA

759

86,095

        10         20         30         40         50         60 
MERIKELRNL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM MAMKYPITAD 

        70         80         90        100        110        120 
KRITEMIPER NEQGQTLWSK MNDAGSDRVM VSPLAVTWWN RNGPITNTVH YPKIYKTYFE 

       130        140        150        160        170        180 
RVERLKHGTF GPVHFRNQVK IRRRVDINPG HADLSAKEAQ DVIMEVVFPN EVGARILTSE 

       190        200        210        220        230        240 
SQLTITKEKK EELQDCKISP LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW 

       250        260        270        280        290        300 
EQMYTPGGEV RNDDVDQSLI IAARNIVRRA AVSADPLASL LEMCHSTQIG GIRMVDILRQ 

       310        320        330        340        350        360 
NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVKR EEEVLTGNLQ TLKIRVHEGY 

       370        380        390        400        410        420 
EEFTMVGRRA TAILRKATRR LIQLIVSGRD EQSIAEAIIV AMVFSQEDCM IKAVRGDLNF 

       430        440        450        460        470        480 
VNRANQRLNP MHQLLRHFQK DAKVLFQNWG VEPIDNVMGM IGILPDMTPS IEMSMRGVRI 

       490        500        510        520        530        540 
SKMGVDEYSS TERVVVSIDR FLRIRDQRGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEIN 

       550        560        570        580        590        600 
GPESVLVNTY QWIIRNWETV KIQWSQNPTM LYNKMEFEPF QSLVPKAIRG QYSGFVRTLF 

       610        620        630        640        650        660 
QQMRDVLGTF DTAQIIKLLP FAAAPPKQSR MQFSSFTVNV RGSGMRILVR GNSPVFNYNK 

       670        680        690        700        710        720 
ATKRLTVLGK DAGTLTEDPD EGTAGVESAV LRGFLILGKE DKRYGPALSI NELSNLAKGE 

       730        740        750 
KANVLIGQGD VVLVMKRKRD SSILTDSQTA TKRIRMAIN

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References

[1]	"Nucleotide sequences of influenza virus segments 1 and 3 reveal mosaic structure of a small viral RNA segment." Fields S., Winter G. Cell 28:303-313(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Plasmid-only rescue of influenza A virus vaccine candidates." Schickli J.H., Flandorfer A., Nakaya T., Martinez-Sobrido L., Garcia-Sastre A., Palese P. Philos. Trans. R. Soc. Lond., B, Biol. Sci. 356:1965-1973(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments." de Wit E., Spronken M.I.J., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D.M.E., Fouchier R.A.M. Virus Res. 103:155-161(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], REVERSE GENETICS.
[4]	"The NIAID influenza genome sequencing project." Ghedin E., Spiro D., Miller N., Zaborsky J., Feldblyum T., Subbu V., Shumway M., Sparenborg J., Groveman L., Halpin R., Sitz J., Koo H., Salzberg S.L., Webster R.G., Hoffmann E., Krauss S., Naeve C., Bao Y. Tatusova T. Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

V00603 Unassigned RNA. Translation: CAA23855.1.
AF389115 Genomic RNA. Translation: AAM75155.1.
EF467818 Genomic RNA. Translation: ABO21705.1.
CY009451 Genomic RNA. Translation: ABD77685.1.

RefSeq

NP_040987.1. NC_002023.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2ZTT	X-ray	2.10	B/D	1-37	[»]
3A1G	X-ray	1.70	B/D	1-37	[»]
3CW4	X-ray	2.70	A	535-759	[»]
4ENF	X-ray	1.32	A	318-483	[»]

ProteinModelPortal

P03428.

SMR

P03428. Positions 319-483, 685-759.

ModBase

Search...

Protein-protein interaction databases

IntAct

P03428. 36 interactions.

MINT

MINT-3375074.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

956536.

Enzyme and pathway databases

Reactome

REACT_116125. Disease.

Family and domain databases

InterPro

IPR001591. RNA_pol_PB2_orthomyxovir.
[Graphical view]

Pfam

PF00604. Flu_PB2. 1 hit.
[Graphical view]

ProDom

PD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Other

EvolutionaryTrace

P03428.

Entry information

Entry name

PB2_I34A1

Accession

Primary (citable) accession number: P03428
Secondary accession number(s): A4GXH0, Q20N28, Q8JUU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	March 6, 2007
Last modified:	May 1, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Enzyme and pathway databases

Family and domain databases

Other

Entry information

Relevant documents