Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P03428

- PB2_I34A1

UniProt

P03428 - PB2_I34A1

Protein

Polymerase basic protein 2

Gene

PB2

Organism
Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 2 (06 Mar 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication By similarity.By similarity

    GO - Molecular functioni

    1. RNA binding Source: InterPro
    2. RNA-directed RNA polymerase activity Source: InterPro

    GO - Biological processi

    1. 7-methylguanosine mRNA capping Source: UniProtKB-KW
    2. cap snatching Source: UniProtKB-KW
    3. fusion of virus membrane with host plasma membrane Source: Reactome
    4. intracellular transport of virus Source: Reactome
    5. receptor-mediated endocytosis of virus by host cell Source: Reactome
    6. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
    7. transcription, DNA-templated Source: InterPro
    8. uncoating of virus Source: Reactome
    9. viral entry into host cell Source: Reactome
    10. viral genome maturation Source: Reactome
    11. viral genome packaging Source: Reactome
    12. viral life cycle Source: Reactome
    13. viral process Source: Reactome
    14. viral release from host cell Source: Reactome
    15. viral transcription Source: Reactome
    16. virion assembly Source: Reactome

    Keywords - Biological processi

    Cap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, mRNA capping, mRNA processing

    Enzyme and pathway databases

    ReactomeiREACT_6145. Influenza Life Cycle.
    REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
    REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
    REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
    REACT_6212. Budding.
    REACT_6235. Packaging of Eight RNA Segments.
    REACT_6248. Transport of Ribonucleoproteins into the Host Nucleus.
    REACT_6321. Uncoating of the Influenza Virion.
    REACT_6326. Release.
    REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_9434. vRNP Assembly.
    REACT_9491. Viral mRNA Translation.
    REACT_9497. vRNA Synthesis.
    REACT_9527. cRNA Synthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polymerase basic protein 2
    Alternative name(s):
    RNA-directed RNA polymerase subunit P3
    Gene namesi
    Name:PB2
    OrganismiInfluenza A virus (strain A/Puerto Rico/8/1934 H1N1)
    Taxonomic identifieri211044 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Homo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000009255: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. endosome lumen Source: Reactome
    3. extracellular region Source: Reactome
    4. host cell mitochondrion Source: UniProtKB-SubCell
    5. host cell nucleus Source: UniProtKB-SubCell
    6. nucleoplasm Source: Reactome
    7. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host mitochondrion, Host nucleus, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 759759Polymerase basic protein 2PRO_0000078834Add
    BLAST

    Interactioni

    Subunit structurei

    Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

    Protein-protein interaction databases

    IntActiP03428. 38 interactions.
    MINTiMINT-3375074.

    Structurei

    Secondary structure

    759
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi1 – 1010
    Helixi14 – 229
    Helixi27 – 337
    Beta strandi323 – 3253
    Beta strandi328 – 3358
    Beta strandi338 – 3458
    Beta strandi351 – 3599
    Beta strandi361 – 3666
    Beta strandi368 – 37710
    Beta strandi380 – 39011
    Helixi391 – 40515
    Helixi408 – 4114
    Helixi430 – 44011
    Helixi443 – 4497
    Beta strandi451 – 4533
    Helixi456 – 4583
    Beta strandi460 – 4634
    Beta strandi469 – 4757
    Beta strandi478 – 4814
    Helixi536 – 5405
    Helixi541 – 55515
    Helixi557 – 56610
    Helixi568 – 5725
    Helixi575 – 5773
    Helixi578 – 5825
    Turni586 – 5883
    Helixi589 – 60517
    Helixi612 – 6187
    Helixi619 – 6224
    Beta strandi634 – 6385
    Beta strandi641 – 6433
    Beta strandi645 – 6517
    Beta strandi656 – 6583
    Beta strandi660 – 6623
    Beta strandi664 – 67411
    Beta strandi687 – 6915
    Beta strandi694 – 6996
    Helixi702 – 7043
    Helixi710 – 7145
    Beta strandi721 – 7277
    Beta strandi730 – 7367

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2ZTTX-ray2.10B/D1-37[»]
    3A1GX-ray1.70B/D1-37[»]
    3CW4X-ray2.70A535-759[»]
    3WI0X-ray2.00A318-484[»]
    3WI1X-ray1.93A318-484[»]
    4ENFX-ray1.32A318-483[»]
    4J2RX-ray2.42A/B318-484[»]
    ProteinModelPortaliP03428.
    SMRiP03428. Positions 319-483, 685-759.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03428.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi736 – 7394Nuclear localization signalBy similarity

    Sequence similaritiesi

    Belongs to the influenza viruses PB2 family.Curated

    Family and domain databases

    InterProiIPR001591. RNA_pol_PB2_orthomyxovir.
    [Graphical view]
    PfamiPF00604. Flu_PB2. 1 hit.
    [Graphical view]
    ProDomiPD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    P03428-1 [UniParc]FASTAAdd to Basket

    « Hide

    MERIKELRNL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM    50
    MAMKYPITAD KRITEMIPER NEQGQTLWSK MNDAGSDRVM VSPLAVTWWN 100
    RNGPITNTVH YPKIYKTYFE RVERLKHGTF GPVHFRNQVK IRRRVDINPG 150
    HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLTITKEKK EELQDCKISP 200
    LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW EQMYTPGGEV 250
    RNDDVDQSLI IAARNIVRRA AVSADPLASL LEMCHSTQIG GIRMVDILRQ 300
    NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVKR EEEVLTGNLQ 350
    TLKIRVHEGY EEFTMVGRRA TAILRKATRR LIQLIVSGRD EQSIAEAIIV 400
    AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKVLFQNWG 450
    VEPIDNVMGM IGILPDMTPS IEMSMRGVRI SKMGVDEYSS TERVVVSIDR 500
    FLRIRDQRGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEIN GPESVLVNTY 550
    QWIIRNWETV KIQWSQNPTM LYNKMEFEPF QSLVPKAIRG QYSGFVRTLF 600
    QQMRDVLGTF DTAQIIKLLP FAAAPPKQSR MQFSSFTVNV RGSGMRILVR 650
    GNSPVFNYNK ATKRLTVLGK DAGTLTEDPD EGTAGVESAV LRGFLILGKE 700
    DKRYGPALSI NELSNLAKGE KANVLIGQGD VVLVMKRKRD SSILTDSQTA 750
    TKRIRMAIN 759
    Length:759
    Mass (Da):86,095
    Last modified:March 6, 2007 - v2
    Checksum:i10D2D1608AE536D2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti105 – 1051I → M in ABD77685. (PubMed:7060132)Curated
    Sequence conflicti251 – 2511R → K in ABD77685. (PubMed:7060132)Curated
    Sequence conflicti299 – 2991R → K in ABD77685. (PubMed:7060132)Curated
    Sequence conflicti309 – 3091D → G in ABD77685. (PubMed:7060132)Curated
    Sequence conflicti504 – 5041I → V in ABD77685. (PubMed:7060132)Curated
    Sequence conflicti701 – 7011D → N in ABO21705. (PubMed:15163504)Curated
    Sequence conflicti702 – 7021K → R in ABD77685. (PubMed:7060132)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00603 Unassigned RNA. Translation: CAA23855.1.
    AF389115 Genomic RNA. Translation: AAM75155.1.
    EF467818 Genomic RNA. Translation: ABO21705.1.
    CY009451 Genomic RNA. Translation: ABD77685.1.
    RefSeqiNP_040987.1. NC_002023.1.

    Genome annotation databases

    GeneIDi956536.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V00603 Unassigned RNA. Translation: CAA23855.1 .
    AF389115 Genomic RNA. Translation: AAM75155.1 .
    EF467818 Genomic RNA. Translation: ABO21705.1 .
    CY009451 Genomic RNA. Translation: ABD77685.1 .
    RefSeqi NP_040987.1. NC_002023.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2ZTT X-ray 2.10 B/D 1-37 [» ]
    3A1G X-ray 1.70 B/D 1-37 [» ]
    3CW4 X-ray 2.70 A 535-759 [» ]
    3WI0 X-ray 2.00 A 318-484 [» ]
    3WI1 X-ray 1.93 A 318-484 [» ]
    4ENF X-ray 1.32 A 318-483 [» ]
    4J2R X-ray 2.42 A/B 318-484 [» ]
    ProteinModelPortali P03428.
    SMRi P03428. Positions 319-483, 685-759.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P03428. 38 interactions.
    MINTi MINT-3375074.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 956536.

    Enzyme and pathway databases

    Reactomei REACT_6145. Influenza Life Cycle.
    REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
    REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
    REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
    REACT_6212. Budding.
    REACT_6235. Packaging of Eight RNA Segments.
    REACT_6248. Transport of Ribonucleoproteins into the Host Nucleus.
    REACT_6321. Uncoating of the Influenza Virion.
    REACT_6326. Release.
    REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_9434. vRNP Assembly.
    REACT_9491. Viral mRNA Translation.
    REACT_9497. vRNA Synthesis.
    REACT_9527. cRNA Synthesis.

    Miscellaneous databases

    EvolutionaryTracei P03428.

    Family and domain databases

    InterProi IPR001591. RNA_pol_PB2_orthomyxovir.
    [Graphical view ]
    Pfami PF00604. Flu_PB2. 1 hit.
    [Graphical view ]
    ProDomi PD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequences of influenza virus segments 1 and 3 reveal mosaic structure of a small viral RNA segment."
      Fields S., Winter G.
      Cell 28:303-313(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    3. "Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments."
      de Wit E., Spronken M.I.J., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D.M.E., Fouchier R.A.M.
      Virus Res. 103:155-161(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], REVERSE GENETICS.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiPB2_I34A1
    AccessioniPrimary (citable) accession number: P03428
    Secondary accession number(s): A4GXH0, Q20N28, Q8JUU8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: March 6, 2007
    Last modified: October 1, 2014
    This is version 96 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3