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P03428

- PB2_I34A1

UniProt

P03428 - PB2_I34A1

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Protein

Polymerase basic protein 2

Gene
PB2
Organism
Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication By similarity.

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. RNA-directed RNA polymerase activity Source: InterPro

GO - Biological processi

  1. 7-methylguanosine mRNA capping Source: UniProtKB-KW
  2. cap snatching Source: UniProtKB-KW
  3. fusion of virus membrane with host plasma membrane Source: Reactome
  4. intracellular transport of virus Source: Reactome
  5. receptor-mediated endocytosis of virus by host cell Source: Reactome
  6. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
  7. transcription, DNA-templated Source: InterPro
  8. uncoating of virus Source: Reactome
  9. viral entry into host cell Source: Reactome
  10. viral genome maturation Source: Reactome
  11. viral genome packaging Source: Reactome
  12. viral life cycle Source: Reactome
  13. viral process Source: Reactome
  14. viral release from host cell Source: Reactome
  15. viral transcription Source: Reactome
  16. virion assembly Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Cap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, mRNA capping, mRNA processing

Enzyme and pathway databases

ReactomeiREACT_6145. Influenza Life Cycle.
REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
REACT_6212. Budding.
REACT_6235. Packaging of Eight RNA Segments.
REACT_6248. Transport of Ribonucleoproteins into the Host Nucleus.
REACT_6321. Uncoating of the Influenza Virion.
REACT_6326. Release.
REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
REACT_6354. Viral Messenger RNA Synthesis.
REACT_9434. vRNP Assembly.
REACT_9491. Viral mRNA Translation.
REACT_9497. vRNA Synthesis.
REACT_9527. cRNA Synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase basic protein 2
Alternative name(s):
RNA-directed RNA polymerase subunit P3
Gene namesi
Name:PB2
OrganismiInfluenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Taxonomic identifieri211044 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000009255: Genome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. endosome lumen Source: Reactome
  3. extracellular region Source: Reactome
  4. host cell mitochondrion Source: UniProtKB-SubCell
  5. host cell nucleus Source: UniProtKB-SubCell
  6. nucleoplasm Source: Reactome
  7. virion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host mitochondrion, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 759759Polymerase basic protein 2PRO_0000078834Add
BLAST

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

Protein-protein interaction databases

IntActiP03428. 38 interactions.
MINTiMINT-3375074.

Structurei

Secondary structure

759
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1 – 1010
Helixi14 – 229
Helixi27 – 337
Beta strandi323 – 3253
Beta strandi328 – 3358
Beta strandi338 – 3458
Beta strandi351 – 3599
Beta strandi361 – 3666
Beta strandi368 – 37710
Beta strandi380 – 39011
Helixi391 – 40515
Helixi408 – 4114
Helixi430 – 44011
Helixi443 – 4497
Beta strandi451 – 4533
Helixi456 – 4583
Beta strandi460 – 4634
Beta strandi469 – 4757
Beta strandi478 – 4814
Helixi536 – 5405
Helixi541 – 55515
Helixi557 – 56610
Helixi568 – 5725
Helixi575 – 5773
Helixi578 – 5825
Turni586 – 5883
Helixi589 – 60517
Helixi612 – 6187
Helixi619 – 6224
Beta strandi634 – 6385
Beta strandi641 – 6433
Beta strandi645 – 6517
Beta strandi656 – 6583
Beta strandi660 – 6623
Beta strandi664 – 67411
Beta strandi687 – 6915
Beta strandi694 – 6996
Helixi702 – 7043
Helixi710 – 7145
Beta strandi721 – 7277
Beta strandi730 – 7367

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ZTTX-ray2.10B/D1-37[»]
3A1GX-ray1.70B/D1-37[»]
3CW4X-ray2.70A535-759[»]
3WI0X-ray2.00A318-484[»]
3WI1X-ray1.93A318-484[»]
4ENFX-ray1.32A318-483[»]
4J2RX-ray2.42A/B318-484[»]
ProteinModelPortaliP03428.
SMRiP03428. Positions 319-483, 685-759.

Miscellaneous databases

EvolutionaryTraceiP03428.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi736 – 7394Nuclear localization signal By similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR001591. RNA_pol_PB2_orthomyxovir.
[Graphical view]
PfamiPF00604. Flu_PB2. 1 hit.
[Graphical view]
ProDomiPD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

P03428-1 [UniParc]FASTAAdd to Basket

« Hide

MERIKELRNL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM    50
MAMKYPITAD KRITEMIPER NEQGQTLWSK MNDAGSDRVM VSPLAVTWWN 100
RNGPITNTVH YPKIYKTYFE RVERLKHGTF GPVHFRNQVK IRRRVDINPG 150
HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLTITKEKK EELQDCKISP 200
LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW EQMYTPGGEV 250
RNDDVDQSLI IAARNIVRRA AVSADPLASL LEMCHSTQIG GIRMVDILRQ 300
NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVKR EEEVLTGNLQ 350
TLKIRVHEGY EEFTMVGRRA TAILRKATRR LIQLIVSGRD EQSIAEAIIV 400
AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKVLFQNWG 450
VEPIDNVMGM IGILPDMTPS IEMSMRGVRI SKMGVDEYSS TERVVVSIDR 500
FLRIRDQRGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEIN GPESVLVNTY 550
QWIIRNWETV KIQWSQNPTM LYNKMEFEPF QSLVPKAIRG QYSGFVRTLF 600
QQMRDVLGTF DTAQIIKLLP FAAAPPKQSR MQFSSFTVNV RGSGMRILVR 650
GNSPVFNYNK ATKRLTVLGK DAGTLTEDPD EGTAGVESAV LRGFLILGKE 700
DKRYGPALSI NELSNLAKGE KANVLIGQGD VVLVMKRKRD SSILTDSQTA 750
TKRIRMAIN 759
Length:759
Mass (Da):86,095
Last modified:March 6, 2007 - v2
Checksum:i10D2D1608AE536D2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti105 – 1051I → M in ABD77685. 1 Publication
Sequence conflicti251 – 2511R → K in ABD77685. 1 Publication
Sequence conflicti299 – 2991R → K in ABD77685. 1 Publication
Sequence conflicti309 – 3091D → G in ABD77685. 1 Publication
Sequence conflicti504 – 5041I → V in ABD77685. 1 Publication
Sequence conflicti701 – 7011D → N in ABO21705. 1 Publication
Sequence conflicti702 – 7021K → R in ABD77685. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V00603 Unassigned RNA. Translation: CAA23855.1.
AF389115 Genomic RNA. Translation: AAM75155.1.
EF467818 Genomic RNA. Translation: ABO21705.1.
CY009451 Genomic RNA. Translation: ABD77685.1.
RefSeqiNP_040987.1. NC_002023.1.

Genome annotation databases

GeneIDi956536.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V00603 Unassigned RNA. Translation: CAA23855.1 .
AF389115 Genomic RNA. Translation: AAM75155.1 .
EF467818 Genomic RNA. Translation: ABO21705.1 .
CY009451 Genomic RNA. Translation: ABD77685.1 .
RefSeqi NP_040987.1. NC_002023.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2ZTT X-ray 2.10 B/D 1-37 [» ]
3A1G X-ray 1.70 B/D 1-37 [» ]
3CW4 X-ray 2.70 A 535-759 [» ]
3WI0 X-ray 2.00 A 318-484 [» ]
3WI1 X-ray 1.93 A 318-484 [» ]
4ENF X-ray 1.32 A 318-483 [» ]
4J2R X-ray 2.42 A/B 318-484 [» ]
ProteinModelPortali P03428.
SMRi P03428. Positions 319-483, 685-759.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P03428. 38 interactions.
MINTi MINT-3375074.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 956536.

Enzyme and pathway databases

Reactomei REACT_6145. Influenza Life Cycle.
REACT_6147. Entry of Influenza Virion into Host Cell via Endocytosis.
REACT_6179. NEP/NS2 Interacts with the Cellular Export Machinery.
REACT_6198. Viral RNP Complexes in the Host Cell Nucleus.
REACT_6212. Budding.
REACT_6235. Packaging of Eight RNA Segments.
REACT_6248. Transport of Ribonucleoproteins into the Host Nucleus.
REACT_6321. Uncoating of the Influenza Virion.
REACT_6326. Release.
REACT_6351. Fusion of the Influenza Virion to the Host Cell Endosome.
REACT_6354. Viral Messenger RNA Synthesis.
REACT_9434. vRNP Assembly.
REACT_9491. Viral mRNA Translation.
REACT_9497. vRNA Synthesis.
REACT_9527. cRNA Synthesis.

Miscellaneous databases

EvolutionaryTracei P03428.

Family and domain databases

InterProi IPR001591. RNA_pol_PB2_orthomyxovir.
[Graphical view ]
Pfami PF00604. Flu_PB2. 1 hit.
[Graphical view ]
ProDomi PD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequences of influenza virus segments 1 and 3 reveal mosaic structure of a small viral RNA segment."
    Fields S., Winter G.
    Cell 28:303-313(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  3. "Efficient generation and growth of influenza virus A/PR/8/34 from eight cDNA fragments."
    de Wit E., Spronken M.I.J., Bestebroer T.M., Rimmelzwaan G.F., Osterhaus A.D.M.E., Fouchier R.A.M.
    Virus Res. 103:155-161(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA], REVERSE GENETICS.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiPB2_I34A1
AccessioniPrimary (citable) accession number: P03428
Secondary accession number(s): A4GXH0, Q20N28, Q8JUU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 6, 2007
Last modified: September 3, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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