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P03427

- PB2_I33A0

UniProt

P03427 - PB2_I33A0

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Protein

Polymerase basic protein 2

Gene

PB2

Organism
Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex. In addition, participates in the inhibition of type I interferon induction through interaction with the host mitochondrial antiviral signaling protein MAVS.By similarity1 Publication

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. RNA-directed RNA polymerase activity Source: InterPro

GO - Biological processi

  1. 7-methylguanosine mRNA capping Source: UniProtKB-KW
  2. cap snatching Source: UniProtKB-KW
  3. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
  4. transcription, DNA-templated Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Cap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host MAVS by virus, Inhibition of host RLR pathway by virus, Inhibition of host RNA polymerase II by virus, mRNA capping, mRNA processing, Viral immunoevasion, Viral transcription

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase basic protein 2
Alternative name(s):
RNA-directed RNA polymerase subunit P3
Gene namesi
Name:PB2
OrganismiInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Taxonomic identifieri381518 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]
ProteomesiUP000000834: Genome

Subcellular locationi

Virion. Host nucleus. Host mitochondrion 2 Publications

GO - Cellular componenti

  1. host cell mitochondrion Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-KW
  3. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host mitochondrion, Host nucleus, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi7 – 71L → A: Complete loss of mitochondrial localization. 1 Publication
Mutagenesisi10 – 101L → A: Complete loss of mitochondrial localization. 1 Publication
Mutagenesisi49 – 491W → A: Partial loss of replication. No effect on transcription. 1 Publication
Mutagenesisi78 – 781W → A: Complete loss of protein expression. 1 Publication
Mutagenesisi130 – 1301F → A: Complete loss of replication. No effect on transcription. 1 Publication
Mutagenesisi130 – 1301F → Y: Enhances viral replication. 1 Publication
Mutagenesisi142 – 1421R → A: Partial loss of replication. No effect on transcription. 1 Publication
Mutagenesisi142 – 1421R → K: Partial loss of replication. No effect on transcription. 1 Publication
Mutagenesisi190 – 1901K → A: No effect on replication or transcription. 1 Publication
Mutagenesisi736 – 7361K → Q: Partial loss of nuclear localization. 1 Publication
Mutagenesisi737 – 7371R → Q: Complete loss of nuclear localization. 1 Publication
Mutagenesisi738 – 7381K → Q: Complete loss of nuclear localization. 1 Publication
Mutagenesisi739 – 7391R → Q: Partial loss of nuclear localization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 759759Polymerase basic protein 2PRO_0000078841Add
BLAST

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus). Interacts with nucleoprotein NP (via N-terminus). Interacts (via N-terminus) with host MAVS (via N-terminus); this interaction inhibits host innate immune response.By similarity1 Publication

Protein-protein interaction databases

IntActiP03427. 2 interactions.
MINTiMINT-8081619.

Structurei

3D structure databases

ProteinModelPortaliP03427.
SMRiP03427. Positions 319-483, 685-759.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi736 – 7394Nuclear localization signal

Sequence similaritiesi

Belongs to the influenza viruses PB2 family.Curated

Family and domain databases

InterProiIPR001591. RNA_pol_PB2_orthomyxovir.
[Graphical view]
PfamiPF00604. Flu_PB2. 1 hit.
[Graphical view]
ProDomiPD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

P03427-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MERIKELRNL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM
60 70 80 90 100
MAMKYPITAD KRITEMIPER NEQGQTLWSK MNDAGSDRVM VSPLAVTWWN
110 120 130 140 150
RNGPVTSTVH YPKIYKTYFE KVERLKHGTF GPVHFRNQVK IRRRVDINPG
160 170 180 190 200
HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLTTTKEKK EELQGCKISP
210 220 230 240 250
LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW EQMYTPGGEA
260 270 280 290 300
RNDDVDQSLI IAARNIVRRA TVSADPLASL LEMCHSTQIG GIRMVNILRQ
310 320 330 340 350
NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVKR EEEVLTGNLQ
360 370 380 390 400
TLKIRVHEGY EEFTMVGRRA TAILRKATRR LIQLIVSGRD EQSIAEAIIV
410 420 430 440 450
AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKALFQNWG
460 470 480 490 500
IESIDNVMGM IGILPDMTPS TEMSMRGVRI SKMGVDEYSS AEKIVVSIDR
510 520 530 540 550
FLRVRDQRGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEIN GPESVLVNTY
560 570 580 590 600
QWIIRNWETV KIQWSQNPTM LYNKMEFEPF QSLVPKAVRG QYSGFVRTLF
610 620 630 640 650
QQMRDVLGTF DTAQIIKLLP FAAAPPKQSG MQFSSLTINV RGSGMRILVR
660 670 680 690 700
GNSPIFNYNK TTKRLTVLGK DAGPLTEDPD EGTAGVESAV LRGFLILGKE
710 720 730 740 750
DRRYGPALSI NELSNLAKGE KANVLIGQGD VVLVMKRKRN SSILTDSQTA

TKRIRMAIN
Length:759
Mass (Da):85,796
Last modified:July 21, 1986 - v1
Checksum:iE7FA3844C44EB62D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02179 Genomic RNA. Translation: AAA43611.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02179 Genomic RNA. Translation: AAA43611.1 .

3D structure databases

ProteinModelPortali P03427.
SMRi P03427. Positions 319-483, 685-759.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P03427. 2 interactions.
MINTi MINT-8081619.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR001591. RNA_pol_PB2_orthomyxovir.
[Graphical view ]
Pfami PF00604. Flu_PB2. 1 hit.
[Graphical view ]
ProDomi PD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence of the polymerase 3 gene of human influenza virus A/WSN/33."
    Kaptein J.S., Nayak D.P.
    J. Virol. 42:55-63(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Two signals mediate nuclear localization of influenza virus (A/WSN/33) polymerase basic protein 2."
    Mukaigawa J., Nayak D.P.
    J. Virol. 65:245-253(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-736; ARG-737; LYS-738 AND ARG-739.
  3. "Mutations in the N-terminal region of influenza virus PB2 protein affect virus RNA replication but not transcription."
    Gastaminza P., Perales B., Falcon A.M., Ortin J.
    J. Virol. 77:5098-5108(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF TRP-49; TRP-78; PHE-130; ARG-142 AND LYS-190.
  4. "Functional domains of the influenza A virus PB2 protein: identification of NP- and PB1-binding sites."
    Poole E., Elton D., Medcalf L., Digard P.
    Virology 321:120-133(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PB1 AND NP.
  5. "Characterization of a mitochondrial-targeting signal in the PB2 protein of influenza viruses."
    Carr S.M., Carnero E., Garcia-Sastre A., Brownlee G.G., Fodor E.
    Virology 344:492-508(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-7 AND LEU-10.

Entry informationi

Entry nameiPB2_I33A0
AccessioniPrimary (citable) accession number: P03427
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 29, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3