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P03427 (PB2_I33A0) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polymerase basic protein 2
Alternative name(s):
RNA-directed RNA polymerase subunit P3
Gene names
Name:PB2
OrganismInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1)) [Complete proteome]
Taxonomic identifier381518 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length759 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication. Ref.4

Subunit structure

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

Subcellular location

Virion. Host nucleus. Host mitochondrion Ref.2 Ref.5.

Sequence similarities

Belongs to the influenza viruses PB2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 759759Polymerase basic protein 2
PRO_0000078841

Regions

Motif736 – 7394Nuclear localization signal

Experimental info

Mutagenesis71L → A: Complete loss of mitochondrial localization. Ref.5
Mutagenesis101L → A: Complete loss of mitochondrial localization. Ref.5
Mutagenesis491W → A: Partial loss of replication. No effect on transcription. Ref.3
Mutagenesis781W → A: Complete loss of protein expression. Ref.3
Mutagenesis1301F → A: Complete loss of replication. No effect on transcription. Ref.3
Mutagenesis1301F → Y: Enhances viral replication. Ref.3
Mutagenesis1421R → A: Partial loss of replication. No effect on transcription. Ref.3
Mutagenesis1421R → K: Partial loss of replication. No effect on transcription. Ref.3
Mutagenesis1901K → A: No effect on replication or transcription. Ref.3
Mutagenesis7361K → Q: Partial loss of nuclear localization. Ref.2
Mutagenesis7371R → Q: Complete loss of nuclear localization. Ref.2
Mutagenesis7381K → Q: Complete loss of nuclear localization. Ref.2
Mutagenesis7391R → Q: Partial loss of nuclear localization. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P03427 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: E7FA3844C44EB62D

FASTA75985,796
        10         20         30         40         50         60 
MERIKELRNL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM MAMKYPITAD 

        70         80         90        100        110        120 
KRITEMIPER NEQGQTLWSK MNDAGSDRVM VSPLAVTWWN RNGPVTSTVH YPKIYKTYFE 

       130        140        150        160        170        180 
KVERLKHGTF GPVHFRNQVK IRRRVDINPG HADLSAKEAQ DVIMEVVFPN EVGARILTSE 

       190        200        210        220        230        240 
SQLTTTKEKK EELQGCKISP LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW 

       250        260        270        280        290        300 
EQMYTPGGEA RNDDVDQSLI IAARNIVRRA TVSADPLASL LEMCHSTQIG GIRMVNILRQ 

       310        320        330        340        350        360 
NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVKR EEEVLTGNLQ TLKIRVHEGY 

       370        380        390        400        410        420 
EEFTMVGRRA TAILRKATRR LIQLIVSGRD EQSIAEAIIV AMVFSQEDCM IKAVRGDLNF 

       430        440        450        460        470        480 
VNRANQRLNP MHQLLRHFQK DAKALFQNWG IESIDNVMGM IGILPDMTPS TEMSMRGVRI 

       490        500        510        520        530        540 
SKMGVDEYSS AEKIVVSIDR FLRVRDQRGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEIN 

       550        560        570        580        590        600 
GPESVLVNTY QWIIRNWETV KIQWSQNPTM LYNKMEFEPF QSLVPKAVRG QYSGFVRTLF 

       610        620        630        640        650        660 
QQMRDVLGTF DTAQIIKLLP FAAAPPKQSG MQFSSLTINV RGSGMRILVR GNSPIFNYNK 

       670        680        690        700        710        720 
TTKRLTVLGK DAGPLTEDPD EGTAGVESAV LRGFLILGKE DRRYGPALSI NELSNLAKGE 

       730        740        750 
KANVLIGQGD VVLVMKRKRN SSILTDSQTA TKRIRMAIN 

« Hide

References

[1]"Complete nucleotide sequence of the polymerase 3 gene of human influenza virus A/WSN/33."
Kaptein J.S., Nayak D.P.
J. Virol. 42:55-63(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Two signals mediate nuclear localization of influenza virus (A/WSN/33) polymerase basic protein 2."
Mukaigawa J., Nayak D.P.
J. Virol. 65:245-253(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-736; ARG-737; LYS-738 AND ARG-739.
[3]"Mutations in the N-terminal region of influenza virus PB2 protein affect virus RNA replication but not transcription."
Gastaminza P., Perales B., Falcon A.M., Ortin J.
J. Virol. 77:5098-5108(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF TRP-49; TRP-78; PHE-130; ARG-142 AND LYS-190.
[4]"Functional domains of the influenza A virus PB2 protein: identification of NP- and PB1-binding sites."
Poole E., Elton D., Medcalf L., Digard P.
Virology 321:120-133(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Characterization of a mitochondrial-targeting signal in the PB2 protein of influenza viruses."
Carr S.M., Carnero E., Garcia-Sastre A., Brownlee G.G., Fodor E.
Virology 344:492-508(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-7 AND LEU-10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02179 Genomic RNA. Translation: AAA43611.1.

3D structure databases

ProteinModelPortalP03427.
SMRP03427. Positions 319-483, 685-759.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP03427. 2 interactions.
MINTMINT-8081619.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001591. RNA_pol_PB2_orthomyxovir.
[Graphical view]
PfamPF00604. Flu_PB2. 1 hit.
[Graphical view]
ProDomPD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry namePB2_I33A0
AccessionPrimary (citable) accession number: P03427
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families