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Protein

Polymerase basic protein 2

Gene

PB2

Organism
Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Recognizes and binds the 7-methylguanosine-containing cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by the viral protein PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex. In addition, participates in the inhibition of type I interferon induction through interaction with and inhibition of the host mitochondrial antiviral signaling protein MAVS.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei627Mammalian adaptationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processCap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host MAVS by virus, Inhibition of host RLR pathway by virus, Inhibition of host RNA polymerase II by virus, mRNA capping, mRNA processing, Viral immunoevasion, Viral transcription

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase basic protein 2UniRule annotation
Alternative name(s):
RNA-directed RNA polymerase subunit P3UniRule annotation
Gene namesi
Name:PB2UniRule annotation
OrganismiInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Taxonomic identifieri381518 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Homo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

  • Virion UniRule annotation
  • Host nucleus UniRule annotation
  • Host mitochondrion UniRule annotation2 Publications

GO - Cellular componenti

  • host cell mitochondrion Source: UniProtKB-SubCell
  • host cell nucleus Source: UniProtKB
  • virion Source: UniProtKB-SubCell

Keywords - Cellular componenti

Host mitochondrion, Host nucleus, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7L → A: Complete loss of mitochondrial localization. 1 Publication1
Mutagenesisi10L → A: Complete loss of mitochondrial localization. 1 Publication1
Mutagenesisi49W → A: Partial loss of replication. No effect on transcription. 1 Publication1
Mutagenesisi78W → A: Complete loss of protein expression. 1 Publication1
Mutagenesisi130F → A: Complete loss of replication. No effect on transcription. 1 Publication1
Mutagenesisi130F → Y: Enhances viral replication. 1 Publication1
Mutagenesisi142R → A: Partial loss of replication. No effect on transcription. 1 Publication1
Mutagenesisi142R → K: Partial loss of replication. No effect on transcription. 1 Publication1
Mutagenesisi190K → A: No effect on replication or transcription. 1 Publication1
Mutagenesisi736K → Q: Partial loss of nuclear localization. 1 Publication1
Mutagenesisi737R → Q: Complete loss of nuclear localization. 1 Publication1
Mutagenesisi738K → Q: Complete loss of nuclear localization. 1 Publication1
Mutagenesisi739R → Q: Partial loss of nuclear localization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000788411 – 759Polymerase basic protein 2Add BLAST759

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus). Interacts with nucleoprotein NP (via N-terminus). Interacts (via N-terminus) with host MAVS (via N-terminus); this interaction inhibits host innate immune response.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TUFMP494119EBI-8430745,EBI-359097From Homo sapiens.

Protein-protein interaction databases

IntActiP03427. 169 interactors.
MINTiMINT-8081619.

Structurei

3D structure databases

ProteinModelPortaliP03427.
SMRiP03427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi736 – 739Nuclear localization signalUniRule annotation4

Sequence similaritiesi

Belongs to the influenza viruses PB2 family.UniRule annotationCurated

Phylogenomic databases

OrthoDBiVOG0900002O.

Family and domain databases

HAMAPiMF_04062. INV_PB2. 1 hit.
InterProiView protein in InterPro
IPR037258. PDB2_C.
IPR001591. RNA_pol_PB2_orthomyxovir.
PfamiView protein in Pfam
PF00604. Flu_PB2. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
SUPFAMiSSF160453. SSF160453. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Polymerase basic protein 2 (identifier: P03427-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERIKELRNL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM
60 70 80 90 100
MAMKYPITAD KRITEMIPER NEQGQTLWSK MNDAGSDRVM VSPLAVTWWN
110 120 130 140 150
RNGPVTSTVH YPKIYKTYFE KVERLKHGTF GPVHFRNQVK IRRRVDINPG
160 170 180 190 200
HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLTTTKEKK EELQGCKISP
210 220 230 240 250
LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW EQMYTPGGEA
260 270 280 290 300
RNDDVDQSLI IAARNIVRRA TVSADPLASL LEMCHSTQIG GIRMVNILRQ
310 320 330 340 350
NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVKR EEEVLTGNLQ
360 370 380 390 400
TLKIRVHEGY EEFTMVGRRA TAILRKATRR LIQLIVSGRD EQSIAEAIIV
410 420 430 440 450
AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKALFQNWG
460 470 480 490 500
IESIDNVMGM IGILPDMTPS TEMSMRGVRI SKMGVDEYSS AEKIVVSIDR
510 520 530 540 550
FLRVRDQRGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEIN GPESVLVNTY
560 570 580 590 600
QWIIRNWETV KIQWSQNPTM LYNKMEFEPF QSLVPKAVRG QYSGFVRTLF
610 620 630 640 650
QQMRDVLGTF DTAQIIKLLP FAAAPPKQSG MQFSSLTINV RGSGMRILVR
660 670 680 690 700
GNSPIFNYNK TTKRLTVLGK DAGPLTEDPD EGTAGVESAV LRGFLILGKE
710 720 730 740 750
DRRYGPALSI NELSNLAKGE KANVLIGQGD VVLVMKRKRN SSILTDSQTA

TKRIRMAIN
Length:759
Mass (Da):85,796
Last modified:July 21, 1986 - v1
Checksum:iE7FA3844C44EB62D
GO
Isoform PB2-S11 Publication (identifier: P0DOG3-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P0DOG3.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:508
Mass (Da):57,652
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02179 Genomic RNA. Translation: AAA43611.1.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPB2_I33A0
AccessioniPrimary (citable) accession number: P03427
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 22, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families