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P03427

- PB2_I33A0

UniProt

P03427 - PB2_I33A0

Protein

Polymerase basic protein 2

Gene

PB2

Organism
Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication.1 Publication

    GO - Molecular functioni

    1. RNA binding Source: InterPro
    2. RNA-directed RNA polymerase activity Source: InterPro

    GO - Biological processi

    1. 7-methylguanosine mRNA capping Source: UniProtKB-KW
    2. cap snatching Source: UniProtKB-KW
    3. suppression by virus of host RNA polymerase II activity Source: UniProtKB-KW
    4. transcription, DNA-templated Source: InterPro

    Keywords - Biological processi

    Cap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host RNA polymerase II by virus, mRNA capping, mRNA processing

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polymerase basic protein 2
    Alternative name(s):
    RNA-directed RNA polymerase subunit P3
    Gene namesi
    Name:PB2
    OrganismiInfluenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1))
    Taxonomic identifieri381518 [NCBI]
    Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
    Virus hostiAves [TaxID: 8782]
    Homo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]
    ProteomesiUP000000834: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. host cell mitochondrion Source: UniProtKB-SubCell
    2. host cell nucleus Source: UniProtKB-SubCell
    3. virion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host mitochondrion, Host nucleus, Virion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi7 – 71L → A: Complete loss of mitochondrial localization. 1 Publication
    Mutagenesisi10 – 101L → A: Complete loss of mitochondrial localization. 1 Publication
    Mutagenesisi49 – 491W → A: Partial loss of replication. No effect on transcription. 1 Publication
    Mutagenesisi78 – 781W → A: Complete loss of protein expression. 1 Publication
    Mutagenesisi130 – 1301F → A: Complete loss of replication. No effect on transcription. 1 Publication
    Mutagenesisi130 – 1301F → Y: Enhances viral replication. 1 Publication
    Mutagenesisi142 – 1421R → A: Partial loss of replication. No effect on transcription. 1 Publication
    Mutagenesisi142 – 1421R → K: Partial loss of replication. No effect on transcription. 1 Publication
    Mutagenesisi190 – 1901K → A: No effect on replication or transcription. 1 Publication
    Mutagenesisi736 – 7361K → Q: Partial loss of nuclear localization. 1 Publication
    Mutagenesisi737 – 7371R → Q: Complete loss of nuclear localization. 1 Publication
    Mutagenesisi738 – 7381K → Q: Complete loss of nuclear localization. 1 Publication
    Mutagenesisi739 – 7391R → Q: Partial loss of nuclear localization. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 759759Polymerase basic protein 2PRO_0000078841Add
    BLAST

    Interactioni

    Subunit structurei

    Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA.

    Protein-protein interaction databases

    IntActiP03427. 2 interactions.
    MINTiMINT-8081619.

    Structurei

    3D structure databases

    ProteinModelPortaliP03427.
    SMRiP03427. Positions 319-483, 685-759.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi736 – 7394Nuclear localization signal

    Sequence similaritiesi

    Belongs to the influenza viruses PB2 family.Curated

    Family and domain databases

    InterProiIPR001591. RNA_pol_PB2_orthomyxovir.
    [Graphical view]
    PfamiPF00604. Flu_PB2. 1 hit.
    [Graphical view]
    ProDomiPD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
    [Graphical view] [Entries sharing at least one domain]

    Sequencei

    Sequence statusi: Complete.

    P03427-1 [UniParc]FASTAAdd to Basket

    « Hide

    MERIKELRNL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPALRMKWM    50
    MAMKYPITAD KRITEMIPER NEQGQTLWSK MNDAGSDRVM VSPLAVTWWN 100
    RNGPVTSTVH YPKIYKTYFE KVERLKHGTF GPVHFRNQVK IRRRVDINPG 150
    HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLTTTKEKK EELQGCKISP 200
    LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW EQMYTPGGEA 250
    RNDDVDQSLI IAARNIVRRA TVSADPLASL LEMCHSTQIG GIRMVNILRQ 300
    NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSVKR EEEVLTGNLQ 350
    TLKIRVHEGY EEFTMVGRRA TAILRKATRR LIQLIVSGRD EQSIAEAIIV 400
    AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKALFQNWG 450
    IESIDNVMGM IGILPDMTPS TEMSMRGVRI SKMGVDEYSS AEKIVVSIDR 500
    FLRVRDQRGN VLLSPEEVSE TQGTEKLTIT YSSSMMWEIN GPESVLVNTY 550
    QWIIRNWETV KIQWSQNPTM LYNKMEFEPF QSLVPKAVRG QYSGFVRTLF 600
    QQMRDVLGTF DTAQIIKLLP FAAAPPKQSG MQFSSLTINV RGSGMRILVR 650
    GNSPIFNYNK TTKRLTVLGK DAGPLTEDPD EGTAGVESAV LRGFLILGKE 700
    DRRYGPALSI NELSNLAKGE KANVLIGQGD VVLVMKRKRN SSILTDSQTA 750
    TKRIRMAIN 759
    Length:759
    Mass (Da):85,796
    Last modified:July 21, 1986 - v1
    Checksum:iE7FA3844C44EB62D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02179 Genomic RNA. Translation: AAA43611.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02179 Genomic RNA. Translation: AAA43611.1 .

    3D structure databases

    ProteinModelPortali P03427.
    SMRi P03427. Positions 319-483, 685-759.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P03427. 2 interactions.
    MINTi MINT-8081619.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001591. RNA_pol_PB2_orthomyxovir.
    [Graphical view ]
    Pfami PF00604. Flu_PB2. 1 hit.
    [Graphical view ]
    ProDomi PD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of the polymerase 3 gene of human influenza virus A/WSN/33."
      Kaptein J.S., Nayak D.P.
      J. Virol. 42:55-63(1982) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Two signals mediate nuclear localization of influenza virus (A/WSN/33) polymerase basic protein 2."
      Mukaigawa J., Nayak D.P.
      J. Virol. 65:245-253(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-736; ARG-737; LYS-738 AND ARG-739.
    3. "Mutations in the N-terminal region of influenza virus PB2 protein affect virus RNA replication but not transcription."
      Gastaminza P., Perales B., Falcon A.M., Ortin J.
      J. Virol. 77:5098-5108(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF TRP-49; TRP-78; PHE-130; ARG-142 AND LYS-190.
    4. "Functional domains of the influenza A virus PB2 protein: identification of NP- and PB1-binding sites."
      Poole E., Elton D., Medcalf L., Digard P.
      Virology 321:120-133(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Characterization of a mitochondrial-targeting signal in the PB2 protein of influenza viruses."
      Carr S.M., Carnero E., Garcia-Sastre A., Brownlee G.G., Fodor E.
      Virology 344:492-508(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-7 AND LEU-10.

    Entry informationi

    Entry nameiPB2_I33A0
    AccessioniPrimary (citable) accession number: P03427
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3