P03427 (PB2_I33A0) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 75.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Polymerase basic protein 2 Alternative name(s): RNA-directed RNA polymerase subunit P3 | ||
| Gene names |
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| Organism | Influenza A virus (strain A/Wilson-Smith/1933 H1N1) (Influenza A virus (strain A/WS/1933 H1N1)) [Complete proteome] | ||
| Taxonomic identifier | 381518 [NCBI] | ||
| Taxonomic lineage | Viruses › ssRNA negative-strand viruses › Orthomyxoviridae › Influenzavirus A ›  | ||
| Virus host | Aves [TaxID: 8782] Homo sapiens (Human) [TaxID: 9606] Sus scrofa (Pig) [TaxID: 9823] |
Protein attributes
| Sequence length | 759 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Involved in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved by PB1. May play a role in genome replication. Ref.4 |
| Subunit structure | Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. |
| Subcellular location | |
| Sequence similarities | Belongs to the influenza viruses PB2 family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 759 | 759 | Polymerase basic protein 2 | PRO_0000078841 | |||||
Regions | |||||||||
| Motif | 736 – 739 | 4 | Nuclear localization signal | ||||||
Experimental info | |||||||||
| Mutagenesis | 7 | 1 | L → A: Complete loss of mitochondrial localization. Ref.5 | ||||||
| Mutagenesis | 10 | 1 | L → A: Complete loss of mitochondrial localization. Ref.5 | ||||||
| Mutagenesis | 49 | 1 | W → A: Partial loss of replication. No effect on transcription. Ref.3 | ||||||
| Mutagenesis | 78 | 1 | W → A: Complete loss of protein expression. Ref.3 | ||||||
| Mutagenesis | 130 | 1 | F → A: Complete loss of replication. No effect on transcription. Ref.3 | ||||||
| Mutagenesis | 130 | 1 | F → Y: Enhances viral replication. Ref.3 | ||||||
| Mutagenesis | 142 | 1 | R → A: Partial loss of replication. No effect on transcription. Ref.3 | ||||||
| Mutagenesis | 142 | 1 | R → K: Partial loss of replication. No effect on transcription. Ref.3 | ||||||
| Mutagenesis | 190 | 1 | K → A: No effect on replication or transcription. Ref.3 | ||||||
| Mutagenesis | 736 | 1 | K → Q: Partial loss of nuclear localization. Ref.2 | ||||||
| Mutagenesis | 737 | 1 | R → Q: Complete loss of nuclear localization. Ref.2 | ||||||
| Mutagenesis | 738 | 1 | K → Q: Complete loss of nuclear localization. Ref.2 | ||||||
| Mutagenesis | 739 | 1 | R → Q: Partial loss of nuclear localization. Ref.2 | ||||||
Sequences
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References
| [1] | "Complete nucleotide sequence of the polymerase 3 gene of human influenza virus A/WSN/33." Kaptein J.S., Nayak D.P. J. Virol. 42:55-63(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. |
| [2] | "Two signals mediate nuclear localization of influenza virus (A/WSN/33) polymerase basic protein 2." Mukaigawa J., Nayak D.P. J. Virol. 65:245-253(1991) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-736; ARG-737; LYS-738 AND ARG-739. |
| [3] | "Mutations in the N-terminal region of influenza virus PB2 protein affect virus RNA replication but not transcription." Gastaminza P., Perales B., Falcon A.M., Ortin J. J. Virol. 77:5098-5108(2003) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF TRP-49; TRP-78; PHE-130; ARG-142 AND LYS-190. |
| [4] | "Functional domains of the influenza A virus PB2 protein: identification of NP- and PB1-binding sites." Poole E., Elton D., Medcalf L., Digard P. Virology 321:120-133(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. |
| [5] | "Characterization of a mitochondrial-targeting signal in the PB2 protein of influenza viruses." Carr S.M., Carnero E., Garcia-Sastre A., Brownlee G.G., Fodor E. Virology 344:492-508(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, MUTAGENESIS OF LEU-7 AND LEU-10. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | J02179 Genomic RNA. Translation: AAA43611.1. |
3D structure databases | |
| ProteinModelPortal | P03427. |
| SMR | P03427. Positions 319-483, 685-759. |
| ModBase | Search... |
Protein-protein interaction databases | |
| MINT | MINT-8081619. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Family and domain databases | |
| InterPro | IPR001591. RNA_pol_PB2_orthomyxovir. [Graphical view] |
| Pfam | PF00604. Flu_PB2. 1 hit. [Graphical view] |
| ProDom | PD001667. RNA_pol_PB2_orthomyxovir. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| ProtoNet | Search... |
Entry information
| Entry name | PB2_I33A0 | ||||||||
| Accession | Primary (citable) accession number: P03427 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Viral Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |