Reviewed, UniProtKB/Swiss-Prot P03425 (HN_SENDH)

Names and origin

Protein names

Recommended name:
    Hemagglutinin-neuraminidase
      Short name=HN protein
    EC=3.2.1.18

Gene names

Name:

HN

Organism

Sendai virus (strain Harris) (SeV)

Taxonomic identifier

11196 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Paramyxoviridae › Paramyxovirinae › Respirovirus

Virus host

Mus musculus (Mouse) [TaxID: 10090]
Rattus norvegicus (Rat) [TaxID: 10116]
Cavia cutleri (Guinea pig) [TaxID: 10144]
Cricetidae sp. (Hamster) [TaxID: 36483]

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of HN protein to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion By similarity. Neuraminidase activity ensures the efficient spread of the virus by dissociating the mature virions from the neuraminic acid containing glycoproteins By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subunit structure	Homotetramer; composed of disulfide-linked homodimers. Interacts with F protein trimer By similarity.
Subcellular location	Virion membrane; Single-pass type II membrane protein Potential. Host cell membrane; Single-pass type II membrane protein Potential. Note: Folded in the endoplasmic reticulum By similarity.
Post-translational modification	N-glycosylated; glycans consist of a mixture of high mannose-type oligosaccharides and of complex-type oligosaccharides By similarity.
Sequence similarities	Belongs to the paramyxoviruses hemagglutinin-neuraminidase family.

Ontologies

Keywords
Cellular component	Cell membrane Envelope protein Membrane Virion
Domain	Signal-anchor Transmembrane
Molecular function	Hemagglutinin Hydrolase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: EC host cell surface receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Hemagglutinin-neuraminidase		PRO_0000142638
Regions
	Topological domain	1 – 37	37	Cytoplasmic By similarity
	Transmembrane	38 – 58	21	By similarity
	Topological domain	59 – 576	518	Extracellular By similarity
	Region	10 – 14	5	Incorporation in virion By similarity
	Region	59 – 140	82	Involved in interaction with F protein By similarity
Amino acid modifications
	Glycosylation	77	1	N-linked (GlcNAc...); by host By similarity
	Glycosylation	499	1	N-linked (GlcNAc...); by host By similarity
	Glycosylation	511	1	N-linked (GlcNAc...); by host By similarity
	Disulfide bond	129		Interchain Potential

Sequences

	Sequence		Length	Mass (Da)
	P03425-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 01CD8067E038FC5B	FASTA	576	63,515
	10 20 30 40 50 60 MDGDRGKRDS YWSTSPSGST TKLASGWERS SKVDTWLLIL SFTQWALSIA TVIICIIISA 70 80 90 100 110 120 RQGYSTKEYS MTVEALNMSS REVKESLTSL IRQEVIARAV NIQSSVQTGI PVLLNKNSRD 130 140 150 160 170 180 VIQMIDKSCS RQELTQLCES TIAVHHAEGI APLEPHSFWR CPVGEPYLSS DPKISLLLGP 190 200 210 220 230 240 SLLSGSTTIS GCVRLPSLSI GEAIYAYSSN LITQGCADIG KSYQVLQLGY ISLNSDMFPD 250 260 270 280 290 300 LNPVVSHTYD INDNRKSCSV VATGTRGYQL CSMPTVDERT DYSSDGIEDL VLDVLDLKGS 310 320 330 340 350 360 TKSHRYRNSE VDLDHPFSAL YPSVGNGIAT EGSLIFLGYG GLTTPLQGDT KCRTQGCQQV 370 380 390 400 410 420 SQDTCNEALK ITWLGGKQVV NVIIRVNDYL SERPKIRVTT IPITQNYLGA EGRLLKLGDR 430 440 450 460 470 480 VYIYTRSSGW HSQLQIGVLD VSHPLTINWT PHEALSRPGN KECNWYNTCP KECISGVYTD 490 500 510 520 530 540 AYPLSPDAAN VATVTLYANT SRVNPTIMYS NTTNIINMLR IKDVQLEVAY TTISSCITHF 550 560 570 GKGYCFHIIE INQKSLNTLQ PMLFKTSIPK LCKAES

References

[1]	"Sequence determination of the Sendai virus HN gene and its comparison to the influenza virus glycoproteins." Blumberg B.M., Giorgi C., Roux L., Raju R., Dowling P., Chollet A., Kolakofsky D. Cell 41:269-278(1985) [PubMed: 2986845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M12397 Genomic RNA. Translation: AAA47810.1.
PIR	HNNZS. A00877.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH83. Glycoside Hydrolase Family 83.
Family and domain databases
InterPro	IPR000665. Hemagglutn-neuramid_glycoprot. IPR016285. Hemagglutn-neuramid_paramyxo. [Graphical view]
Pfam	PF00423. HN. 1 hit. [Graphical view]
PIRSF	PIRSF001072. Hemagglut-neuramid_paramyxoV. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HN_SENDH

Accession

Primary (citable) accession number: P03425

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)