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P03418 (NCAP_HRSVA) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nucleoprotein

Short name=Protein N
Alternative name(s):
Nucleocapsid protein
Gene names
Name:N
OrganismHuman respiratory syncytial virus A (strain A2) [Complete proteome]
Taxonomic identifier11259 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesMononegaviralesParamyxoviridaePneumovirinaePneumovirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length391 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Encapsidates the genome, protecting it from nucleases. The nucleocapsid (NC) has a helical structure. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by protein N is coupled to RNA synthesis and all replicative products are resistant to nucleases. Ref.7

Subunit structure

Homomultimerizes to form the nucleocapsid. Binds to viral genomic RNA. In nucleocapsid, interacts with the protein P and thereby positions the polymerase on the template. Interacts with protein M2-1; this interaction allows the association of nucleocapsid with the matrix protein, supposely to shut down virus transcriptase activity and initiate assembly and budding. Ref.8 Ref.9

Subcellular location

Virion. Host cytoplasm.

Sequence similarities

Belongs to the paramyxoviruses nucleocapsid family.

Sequence caution

The sequence CAA24906.1 differs from that shown. Reason: Frameshift at position 376.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MAVSM1QCQ23EBI-6930799,EBI-6930883From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 391391Nucleoprotein
PRO_0000142652

Natural variations

Natural variant2671V → I in strain: Cold-passage attenuated.

Secondary structure

.................................................... 391
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P03418 [UniParc].

Last modified April 1, 1988. Version 1.
Checksum: D06E84F4F88D382B

FASTA39143,451
        10         20         30         40         50         60 
MALSKVKLND TLNKDQLLSS SKYTIQRSTG DSIDTPNYDV QKHINKLCGM LLITEDANHK 

        70         80         90        100        110        120 
FTGLIGMLYA MSRLGREDTI KILRDAGYHV KANGVDVTTH RQDINGKEMK FEVLTLASLT 

       130        140        150        160        170        180 
TEIQINIEIE SRKSYKKMLK EMGEVAPEYR HDSPDCGMII LCIAALVITK LAAGDRSGLT 

       190        200        210        220        230        240 
AVIRRANNVL KNEMKRYKGL LPKDIANSFY EVFEKHPHFI DVFVHFGIAQ SSTRGGSRVE 

       250        260        270        280        290        300 
GIFAGLFMNA YGAGQVMLRW GVLAKSVKNI MLGHASVQAE MEQVVEVYEY AQKLGGEAGF 

       310        320        330        340        350        360 
YHILNNPKAS LLSLTQFPHF SSVVLGNAAG LGIMGEYRGT PRNQDLYDAA KAYAEQLKEN 

       370        380        390 
GVINYSVLDL TAEELEAIKH QLNPKDNDVE L 

« Hide

References

[1]"Correct sequence for the major nucleocapsid protein mRNA of respiratory syncytial virus."
Collins P.L., Anderson K., Langer S.J., Wertz G.W.
Virology 146:69-77(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Amino acid sequence of human respiratory syncytial virus nucleocapsid protein."
Elango N., Venkatesan S.
Nucleic Acids Res. 11:5941-5951(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]"A cold-passaged, attenuated strain of human respiratory syncytial virus contains mutations in the F and L genes."
Connors M., Crowe J.E. Jr., Firestone C.Y., Murphy B.R., Collins P.L.
Virology 208:478-484(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Cold-passage attenuated.
[4]"Acquisition of the ts phenotype by a chemically mutagenized cold-passaged human respiratory syncytial virus vaccine candidate results from the acquisition of a single mutation in the polymerase (L) gene."
Crowe J.E. Jr., Firestone C.Y., Whitehead S.S., Collins P.L., Murphy B.R.
Virus Genes 13:269-273(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Cold-passage attenuated.
[5]"Nucleotide sequence analysis of the respiratory syncytial virus subgroup A cold-passaged (cp) temperature sensitive (ts) cpts-248/404 live attenuated virus vaccine candidate."
Firestone C.Y., Whitehead S.S., Collins P.L., Murphy B.R., Crowe J.E. Jr.
Virology 225:419-422(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Cold-passage attenuated.
[6]"Recombinant respiratory syncytial virus (RSV) bearing a set of mutations from cold-passaged RSV is attenuated in chimpanzees."
Whitehead S.S., Juhasz K., Firestone C.Y., Collins P.L., Murphy B.R.
J. Virol. 72:4467-4471(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
Strain: Cold-passage attenuated.
[7]"Increased expression of the N protein of respiratory syncytial virus stimulates minigenome replication but does not alter the balance between the synthesis of mRNA and antigenome."
Fearns R., Peeples M.E., Collins P.L.
Virology 236:188-201(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"The Cys(3)-His(1) motif of the respiratory syncytial virus M2-1 protein is essential for protein function."
Hardy R.W., Wertz G.W.
J. Virol. 74:5880-5885(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH M2-1.
[9]"Identification of temperature-sensitive mutations in the phosphoprotein of respiratory syncytial virus that are likely involved in its interaction with the nucleoprotein."
Lu B., Brazas R., Ma C.H., Kristoff T., Cheng X., Jin H.
J. Virol. 76:2871-2880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH P.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M11486 Genomic RNA. Translation: AAB59852.1.
X00001 Genomic RNA. Translation: CAA24906.1. Frameshift.
U50362 Genomic RNA. Translation: AAB86658.1.
U50363 Genomic RNA. Translation: AAB86670.1.
U63644 Genomic RNA. Translation: AAC55964.1.
AF035006 Genomic RNA. Translation: AAC14896.1.
PIRVHNZ. A04026.
VHNZ1. A23316.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2WJ8X-ray3.29A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T1-391[»]
2YHMX-ray3.60A/B/C/D/E/F/G/H/I/J1-375[»]
4BKKelectron microscopy-B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X1-391[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP03418. 4 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR004930. Pneumo_ncap.
[Graphical view]
PfamPF03246. Pneumo_ncap. 1 hit.
[Graphical view]
ProDomPD006438. Pneumo_ncap. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Other

EvolutionaryTraceP03418.

Entry information

Entry nameNCAP_HRSVA
AccessionPrimary (citable) accession number: P03418
Secondary accession number(s): P88810
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1988
Last modified: June 11, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references