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P03394

- ENV_FRSFL

UniProt

P03394 - ENV_FRSFL

Protein

Glycoprotein 55

Gene

env

Organism
Friend spleen focus-forming virus (strain Lilly-Steeves) (FSFFV)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    This envelope-like membrane glycoprotein is responsible for ligand-independent activation of the erythropoietin receptor EPOR leading to the abnormally rapid proliferation of erythroid precursor cells. In the first stage of Friend disease, constitutive activation of EPOR by gp55 causes uncontrolled, polyclonal proliferation of infected erythroblasts, leading to polycythemia (massive increase in the number of mature red cells). Host susceptibility to SSFV-induced erythroblastosis depends on the expression of the truncated isoform of MST1R receptor tyrosine kinase (MST1R isoform sf-Stk). Interaction with SSFV gp 55 results in constitutive tyrosine phosphorylation and activation of MST1R isoform sf-Stk By similarity.By similarity

    GO - Molecular functioni

    1. structural molecule activity Source: InterPro

    GO - Biological processi

    1. fusion of virus membrane with host plasma membrane Source: UniProtKB-KW
    2. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Fusion of virus membrane with host cell membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycoprotein 55
    Short name:
    gp55
    Gene namesi
    Name:env
    OrganismiFriend spleen focus-forming virus (strain Lilly-Steeves) (FSFFV)
    Taxonomic identifieri355328 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
    Virus hostiMus [TaxID: 10088]

    Subcellular locationi

    Host endoplasmic reticulum membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. Virion membrane; Single-pass type I membrane protein
    Note: The envelope-like membrane glycoprotein gp55 is defective in its transport to the cell surface and remains associated predominantly with the rough endoplasmic reticulum (RER) membrane. It is almost not incorporated into virions. Host cell surface expression appears to be a prerequisite for its leukemogenicity By similarity.By similarity

    GO - Cellular componenti

    1. host cell endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. host cell plasma membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. viral capsid Source: InterPro
    5. viral envelope Source: UniProtKB-KW
    6. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host endoplasmic reticulum, Host membrane, Membrane, Viral envelope protein, Virion

    Pathology & Biotechi

    Keywords - Diseasei

    Oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Sequence AnalysisAdd
    BLAST
    Chaini33 – 409377Glycoprotein 55PRO_0000040720Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi43 – 431N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi58 – 581N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi296 – 2961N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi328 – 3281N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi377 – 3771N-linked (GlcNAc...); by hostSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Subunit structurei

    Homooligomer. Forms heterooligomers with mouse EPOR, probably via their respective transmembrane domains. Forms covalent heterodimers with mouse MST1R isoform sf-Stk, probably via disulfide bonds By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP03394.
    SMRiP03394. Positions 38-229.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini33 – 384352Virion surfaceSequence AnalysisAdd
    BLAST
    Topological domaini406 – 4094IntravirionSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei385 – 40521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi229 – 27850Pro-richAdd
    BLAST

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di3.90.310.10. 1 hit.
    InterProiIPR008981. FMuLV_rcpt-bd.
    IPR018154. TLV/ENV_coat_polyprotein.
    [Graphical view]
    PANTHERiPTHR10424. PTHR10424. 1 hit.
    PfamiPF00429. TLV_coat. 3 hits.
    [Graphical view]
    SUPFAMiSSF49830. SSF49830. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P03394-1 [UniParc]FASTAAdd to Basket

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    MEGPASSKPL KDKTNPWGPL IILGILIRAG VSVQLDSPHQ VSNVTWRVTN    50
    LMTGQTANAT SLLGTMTEAF PKLYFDLCDL MGDDWDETGL GCRTPGGRKR 100
    ARTFDFYVCP GHTVPTGCGG PREGYCGKWG CETTGQAYWK PSSSWDLISL 150
    KRGNTPKDQG PCYDSSVSSG VLGATPGGRC NPLVLEFTDA GRKASWDAPK 200
    VWGLRLYRST GTDPVTRFSL TRQVLDIGPR VPIGSNPVTT DQLPLSRPVQ 250
    TMPPRPLQPP PPGAASIVPE TAPPPQQPGA GDRLLNLVDG AYQALNLTNP 300
    DKIQECWLCL VSGPPYYEGV VVLGTYFNHT IALKEKCCFY ADHTGLVRDS 350
    MAKLRKRLTQ RQKLFESSRG WFEGSSNRSP WFTTLISAIM GSLIILLLLL 400
    ILLIWTLYS 409
    Length:409
    Mass (Da):44,700
    Last modified:July 21, 1986 - v1
    Checksum:i2D12505183293B3A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01552 Genomic DNA. Translation: CAA24793.1.
    PIRiA03994. VCVW2S.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01552 Genomic DNA. Translation: CAA24793.1 .
    PIRi A03994. VCVW2S.

    3D structure databases

    ProteinModelPortali P03394.
    SMRi P03394. Positions 38-229.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.90.310.10. 1 hit.
    InterProi IPR008981. FMuLV_rcpt-bd.
    IPR018154. TLV/ENV_coat_polyprotein.
    [Graphical view ]
    PANTHERi PTHR10424. PTHR10424. 1 hit.
    Pfami PF00429. TLV_coat. 3 hits.
    [Graphical view ]
    SUPFAMi SSF49830. SSF49830. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Envelope gene of the Friend spleen focus-forming virus: deletion and insertions in 3' gp70/p15E-encoding region have resulted in unique features in the primary structure of its protein product."
      Wolff L., Scolnick E., Ruscetti S.
      Proc. Natl. Acad. Sci. U.S.A. 80:4718-4722(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiENV_FRSFL
    AccessioniPrimary (citable) accession number: P03394
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Compared to other gammaretroviruses which possess 2 envelope proteins (gp70 and p15E), gp55 corresponds to a gp70-p15E fusion protein with a deletion of a portion of p15E. It is encoded by the defective env gene of the virus.
    The Friend murine leukemia virus complex induces a rapid and fatal erythroleukemia in adult mice. It is the replication-defective spleen focus-forming virus (SFFV) contained in this complex that causes foci of proliferating erythroid cells in spleens of infected mice. The second component is a replication competent Friend murine leukemia virus (F-MuLV) that serves as a helper virus for SFFV.

    External Data

    Dasty 3