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P03394

- ENV_FRSFL

UniProt

P03394 - ENV_FRSFL

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Protein

Glycoprotein 55

Gene

env

Organism
Friend spleen focus-forming virus (strain Lilly-Steeves) (FSFFV)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

This envelope-like membrane glycoprotein is responsible for ligand-independent activation of the erythropoietin receptor EPOR leading to the abnormally rapid proliferation of erythroid precursor cells. In the first stage of Friend disease, constitutive activation of EPOR by gp55 causes uncontrolled, polyclonal proliferation of infected erythroblasts, leading to polycythemia (massive increase in the number of mature red cells). Host susceptibility to SSFV-induced erythroblastosis depends on the expression of the truncated isoform of MST1R receptor tyrosine kinase (MST1R isoform sf-Stk). Interaction with SSFV gp 55 results in constitutive tyrosine phosphorylation and activation of MST1R isoform sf-Stk (By similarity).By similarity

GO - Molecular functioni

  1. structural molecule activity Source: InterPro

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host cell membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Glycoprotein 55
Short name:
gp55
Gene namesi
Name:env
OrganismiFriend spleen focus-forming virus (strain Lilly-Steeves) (FSFFV)
Taxonomic identifieri355328 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
Virus hostiMus [TaxID: 10088]

Subcellular locationi

Host endoplasmic reticulum membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. Virion membrane; Single-pass type I membrane protein
Note: The envelope-like membrane glycoprotein gp55 is defective in its transport to the cell surface and remains associated predominantly with the rough endoplasmic reticulum (RER) membrane. It is almost not incorporated into virions. Host cell surface expression appears to be a prerequisite for its leukemogenicity (By similarity).By similarity

GO - Cellular componenti

  1. host cell endoplasmic reticulum Source: UniProtKB-KW
  2. host cell plasma membrane Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
  4. viral capsid Source: InterPro
  5. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host endoplasmic reticulum, Host membrane, Membrane, Viral envelope protein, Virion

Pathology & Biotechi

Keywords - Diseasei

Oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence AnalysisAdd
BLAST
Chaini33 – 409377Glycoprotein 55PRO_0000040720Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi43 – 431N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi58 – 581N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi296 – 2961N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi328 – 3281N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi377 – 3771N-linked (GlcNAc...); by hostSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homooligomer. Forms heterooligomers with mouse EPOR, probably via their respective transmembrane domains. Forms covalent heterodimers with mouse MST1R isoform sf-Stk, probably via disulfide bonds (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP03394.
SMRiP03394. Positions 38-229.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini33 – 384352Virion surfaceSequence AnalysisAdd
BLAST
Topological domaini406 – 4094IntravirionSequence Analysis

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei385 – 40521HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi229 – 27850Pro-richAdd
BLAST

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.90.310.10. 1 hit.
InterProiIPR008981. FMuLV_rcpt-bd.
IPR018154. TLV/ENV_coat_polyprotein.
[Graphical view]
PANTHERiPTHR10424. PTHR10424. 1 hit.
PfamiPF00429. TLV_coat. 3 hits.
[Graphical view]
SUPFAMiSSF49830. SSF49830. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03394-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEGPASSKPL KDKTNPWGPL IILGILIRAG VSVQLDSPHQ VSNVTWRVTN
60 70 80 90 100
LMTGQTANAT SLLGTMTEAF PKLYFDLCDL MGDDWDETGL GCRTPGGRKR
110 120 130 140 150
ARTFDFYVCP GHTVPTGCGG PREGYCGKWG CETTGQAYWK PSSSWDLISL
160 170 180 190 200
KRGNTPKDQG PCYDSSVSSG VLGATPGGRC NPLVLEFTDA GRKASWDAPK
210 220 230 240 250
VWGLRLYRST GTDPVTRFSL TRQVLDIGPR VPIGSNPVTT DQLPLSRPVQ
260 270 280 290 300
TMPPRPLQPP PPGAASIVPE TAPPPQQPGA GDRLLNLVDG AYQALNLTNP
310 320 330 340 350
DKIQECWLCL VSGPPYYEGV VVLGTYFNHT IALKEKCCFY ADHTGLVRDS
360 370 380 390 400
MAKLRKRLTQ RQKLFESSRG WFEGSSNRSP WFTTLISAIM GSLIILLLLL

ILLIWTLYS
Length:409
Mass (Da):44,700
Last modified:July 21, 1986 - v1
Checksum:i2D12505183293B3A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01552 Genomic DNA. Translation: CAA24793.1.
PIRiA03994. VCVW2S.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01552 Genomic DNA. Translation: CAA24793.1 .
PIRi A03994. VCVW2S.

3D structure databases

ProteinModelPortali P03394.
SMRi P03394. Positions 38-229.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.90.310.10. 1 hit.
InterProi IPR008981. FMuLV_rcpt-bd.
IPR018154. TLV/ENV_coat_polyprotein.
[Graphical view ]
PANTHERi PTHR10424. PTHR10424. 1 hit.
Pfami PF00429. TLV_coat. 3 hits.
[Graphical view ]
SUPFAMi SSF49830. SSF49830. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Envelope gene of the Friend spleen focus-forming virus: deletion and insertions in 3' gp70/p15E-encoding region have resulted in unique features in the primary structure of its protein product."
    Wolff L., Scolnick E., Ruscetti S.
    Proc. Natl. Acad. Sci. U.S.A. 80:4718-4722(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiENV_FRSFL
AccessioniPrimary (citable) accession number: P03394
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 29, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Compared to other gammaretroviruses which possess 2 envelope proteins (gp70 and p15E), gp55 corresponds to a gp70-p15E fusion protein with a deletion of a portion of p15E. It is encoded by the defective env gene of the virus.
The Friend murine leukemia virus complex induces a rapid and fatal erythroleukemia in adult mice. It is the replication-defective spleen focus-forming virus (SFFV) contained in this complex that causes foci of proliferating erythroid cells in spleens of infected mice. The second component is a replication competent Friend murine leukemia virus (F-MuLV) that serves as a helper virus for SFFV.

External Data

Dasty 3