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P03393 (ENV_FRSF5) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glycoprotein 55

Short name=gp55
Gene names
Name:env
OrganismFriend spleen focus-forming virus (isolate 502) (FSFFV)
Taxonomic identifier355329 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
Virus hostMus [TaxID: 10088]

Protein attributes

Sequence length409 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This envelope-like membrane glycoprotein is responsible for ligand-independent activation of the erythropoietin receptor EPOR leading to the abnormally rapid proliferation of erythroid precursor cells. In the first stage of Friend disease, constitutive activation of EPOR by gp55 causes uncontrolled, polyclonal proliferation of infected erythroblasts, leading to polycythemia (massive increase in the number of mature red cells). Host susceptibility to SSFV-induced erythroblastosis depends on the expression of the truncated isoform ofMST1R receptor tyrosine kinase (MST1R isoform sf-Stk) Interaction with SSFV gp 55 results in constitutive tyrosine phosphorylation and activation of MST1R isoform sf-Stk Ref.5 Ref.6

Subunit structure

Homooligomer Probable. Forms heterooligomers with mouse EPOR, probably via their respective transmembrane domains. Forms covalent heterodimers with mouse MST1R isoform sf-Stk probably via disulfide bonds. Ref.6

Subcellular location

Host endoplasmic reticulum membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. Virion membrane; Single-pass type I membrane protein Probable. Note: The envelope-like membrane glycoprotein gp55 is defective in its transport to the cell surface and remains associated predominantly with the rough endoplasmic reticulum (RER) membrane. It is almost not incorporated into virions. Host cell surface expression appears to be a prerequisite for its leukemogenicity.

Miscellaneous

Compared to other gammaretroviruses which possess 2 envelope proteins (gp70 and p15E), gp55 corresponds to a gp70-p15E fusion protein with a deletion of a portion of p15E. It is encoded by the defective env gene of the virus.

The Friend murine leukemia virus complex induces a rapid and fatal erythroleukemia in adult mice. It is the replication-defective spleen focus-forming virus (SFFV) contained in this complex that causes foci of proliferating erythroid cells in spleens of infected mice. The second component is a replication competent Friend murine leukemia virus (F-MuLV) that serves as a helper virus for SFFV.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential
Chain33 – 409377Glycoprotein 55
PRO_0000040718

Regions

Topological domain33 – 384352Virion surface Potential
Transmembrane385 – 40521Helical; Potential
Topological domain406 – 4094Intravirion Potential
Compositional bias229 – 27850Pro-rich

Amino acid modifications

Glycosylation431N-linked (GlcNAc...); by host Potential
Glycosylation581N-linked (GlcNAc...); by host Potential
Glycosylation2961N-linked (GlcNAc...); by host Potential
Glycosylation3281N-linked (GlcNAc...); by host Potential
Glycosylation3771N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
P03393 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 407F56F769863D74

FASTA40944,777
        10         20         30         40         50         60 
MKGPAFSKPL KDKINPWGPL IVLGILIRAG VSVQHDSPHQ VFNVTWRVTN LMTGQTANAT 

        70         80         90        100        110        120 
SLLGTMTDAF PMLHFDLCDL IGDDWDETGL ECRTPGGRKR ARTFDFYVCP GHTVPTGCGG 

       130        140        150        160        170        180 
PREGYCGKWG CETTGQAYWK PSSSWDLISL KRGNTPKDRG PCYDSSVSSG VQGATPGGRC 

       190        200        210        220        230        240 
NPLVLKFTDA GKKASWDSPK VWGLRLYRPT GIDPVTRFSL TRQVLNIGPR IPIGPNPVII 

       250        260        270        280        290        300 
GQLPPSRPVQ VRLPRPPQPP PTGAASMVPG TAPPSQQPGT GDRLLNLVQG AYQALNLTNP 

       310        320        330        340        350        360 
DKTQECWLCL VSGPPYYEGV AVLGTNSNHT SALKEKCCFY ADHTGLVRDS MAKLRKRLTQ 

       370        380        390        400 
RQKLFESSQG WFEGSFNRSP WFTTLISTIM GLLIILLLLL ILLLWTLHS 

« Hide

References

[1]"Complete nucleotide sequence of an infectious clone of Friend spleen focus-forming provirus: gp55 is an envelope fusion glycoprotein."
Clark S.P., Mak T.W.
Proc. Natl. Acad. Sci. U.S.A. 80:5037-5041(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"A deletion in the Friend spleen focus-forming virus env gene is necessary for its product (gp55) to be leukemogenic."
Watanabe N., Nishi M., Ikawa Y., Amanuma H.
J. Virol. 64:2678-2686(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[3]Erratum
Watanabe N., Nishi M., Ikawa Y., Amanuma H.
J. Virol. 64:5694-5694(1990)
[4]"Friend erythroleukemia revisited."
Ney P.A., D'Andrea A.D.
Blood 96:3675-3680(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[5]"The envelope glycoprotein of friend spleen focus-forming virus covalently interacts with and constitutively activates a truncated form of the receptor tyrosine kinase Stk."
Nishigaki K., Thompson D., Hanson C., Yugawa T., Ruscetti S.
J. Virol. 75:7893-7903(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MOUSE MST1R ISOFORM SF-STK.
[6]"The erythropoietin receptor transmembrane domain mediates complex formation with viral anemic and polycythemic gp55 proteins."
Constantinescu S.N., Keren T., Russ W.P., Ubarretxena-Belandia I., Malka Y., Kubatzky K.F., Engelman D.M., Lodish H.F., Henis Y.I.
J. Biol. Chem. 278:43755-43763(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, INTERACTION WITH MOUSE EPOR.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K00021 Genomic RNA. Translation: AAA46487.1.
PIRVCVW5S. A03993.
RefSeqNP_041218.1. NC_001500.1.

3D structure databases

ProteinModelPortalP03393.
SMRP03393. Positions 38-229.
ModBaseSearch...
MobiDBSearch...

PTM databases

UniCarbKBP03393.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1491889.

Family and domain databases

Gene3D3.90.310.10. 1 hit.
InterProIPR008981. FMuLV_rcpt-bd.
IPR018154. TLV/ENV_coat_polyprotein.
[Graphical view]
PANTHERPTHR10424. PTHR10424. 1 hit.
PfamPF00429. TLV_coat. 3 hits.
[Graphical view]
SUPFAMSSF49830. SSF49830. 1 hit.
ProtoNetSearch...

Entry information

Entry nameENV_FRSF5
AccessionPrimary (citable) accession number: P03393
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: February 19, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program