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P03393

- ENV_FRSF5

UniProt

P03393 - ENV_FRSF5

Protein

Glycoprotein 55

Gene

env

Organism
Friend spleen focus-forming virus (isolate 502) (FSFFV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    This envelope-like membrane glycoprotein is responsible for ligand-independent activation of the erythropoietin receptor EPOR leading to the abnormally rapid proliferation of erythroid precursor cells. In the first stage of Friend disease, constitutive activation of EPOR by gp55 causes uncontrolled, polyclonal proliferation of infected erythroblasts, leading to polycythemia (massive increase in the number of mature red cells). Host susceptibility to SSFV-induced erythroblastosis depends on the expression of the truncated isoform of MST1R receptor tyrosine kinase (MST1R isoform sf-Stk). Interaction with SSFV gp 55 results in constitutive tyrosine phosphorylation and activation of MST1R isoform sf-Stk.2 Publications

    GO - Molecular functioni

    1. structural molecule activity Source: InterPro

    GO - Biological processi

    1. fusion of virus membrane with host plasma membrane Source: UniProtKB-KW
    2. virion attachment to host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Fusion of virus membrane with host cell membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycoprotein 55
    Short name:
    gp55
    Gene namesi
    Name:env
    OrganismiFriend spleen focus-forming virus (isolate 502) (FSFFV)
    Taxonomic identifieri355329 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
    Virus hostiMus [TaxID: 10088]

    Subcellular locationi

    Host endoplasmic reticulum membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. Virion membrane Curated; Single-pass type I membrane protein Curated
    Note: The envelope-like membrane glycoprotein gp55 is defective in its transport to the cell surface and remains associated predominantly with the rough endoplasmic reticulum (RER) membrane. It is almost not incorporated into virions. Host cell surface expression appears to be a prerequisite for its leukemogenicity.

    GO - Cellular componenti

    1. host cell endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. host cell plasma membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. viral capsid Source: InterPro
    5. viral envelope Source: UniProtKB-KW
    6. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Host cell membrane, Host endoplasmic reticulum, Host membrane, Membrane, Viral envelope protein, Virion

    Pathology & Biotechi

    Keywords - Diseasei

    Oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Sequence AnalysisAdd
    BLAST
    Chaini33 – 409377Glycoprotein 55PRO_0000040718Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi43 – 431N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi58 – 581N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi296 – 2961N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi328 – 3281N-linked (GlcNAc...); by hostSequence Analysis
    Glycosylationi377 – 3771N-linked (GlcNAc...); by hostSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    PTM databases

    UniCarbKBiP03393.

    Interactioni

    Subunit structurei

    Homooligomer Probable. Forms heterooligomers with mouse EPOR, probably via their respective transmembrane domains. Forms covalent heterodimers with mouse MST1R isoform sf-Stk, probably via disulfide bonds.1 PublicationCurated

    Structurei

    3D structure databases

    ProteinModelPortaliP03393.
    SMRiP03393. Positions 38-229.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini33 – 384352Virion surfaceSequence AnalysisAdd
    BLAST
    Topological domaini406 – 4094IntravirionSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei385 – 40521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi229 – 27850Pro-richAdd
    BLAST

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di3.90.310.10. 1 hit.
    InterProiIPR008981. FMuLV_rcpt-bd.
    IPR018154. TLV/ENV_coat_polyprotein.
    [Graphical view]
    PANTHERiPTHR10424. PTHR10424. 1 hit.
    PfamiPF00429. TLV_coat. 3 hits.
    [Graphical view]
    SUPFAMiSSF49830. SSF49830. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P03393-1 [UniParc]FASTAAdd to Basket

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    MKGPAFSKPL KDKINPWGPL IVLGILIRAG VSVQHDSPHQ VFNVTWRVTN    50
    LMTGQTANAT SLLGTMTDAF PMLHFDLCDL IGDDWDETGL ECRTPGGRKR 100
    ARTFDFYVCP GHTVPTGCGG PREGYCGKWG CETTGQAYWK PSSSWDLISL 150
    KRGNTPKDRG PCYDSSVSSG VQGATPGGRC NPLVLKFTDA GKKASWDSPK 200
    VWGLRLYRPT GIDPVTRFSL TRQVLNIGPR IPIGPNPVII GQLPPSRPVQ 250
    VRLPRPPQPP PTGAASMVPG TAPPSQQPGT GDRLLNLVQG AYQALNLTNP 300
    DKTQECWLCL VSGPPYYEGV AVLGTNSNHT SALKEKCCFY ADHTGLVRDS 350
    MAKLRKRLTQ RQKLFESSQG WFEGSFNRSP WFTTLISTIM GLLIILLLLL 400
    ILLLWTLHS 409
    Length:409
    Mass (Da):44,777
    Last modified:July 21, 1986 - v1
    Checksum:i407F56F769863D74
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K00021 Genomic RNA. Translation: AAA46487.1.
    PIRiA03993. VCVW5S.
    RefSeqiNP_041218.1. NC_001500.1.

    Genome annotation databases

    GeneIDi1491889.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K00021 Genomic RNA. Translation: AAA46487.1 .
    PIRi A03993. VCVW5S.
    RefSeqi NP_041218.1. NC_001500.1.

    3D structure databases

    ProteinModelPortali P03393.
    SMRi P03393. Positions 38-229.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    UniCarbKBi P03393.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1491889.

    Family and domain databases

    Gene3Di 3.90.310.10. 1 hit.
    InterProi IPR008981. FMuLV_rcpt-bd.
    IPR018154. TLV/ENV_coat_polyprotein.
    [Graphical view ]
    PANTHERi PTHR10424. PTHR10424. 1 hit.
    Pfami PF00429. TLV_coat. 3 hits.
    [Graphical view ]
    SUPFAMi SSF49830. SSF49830. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of an infectious clone of Friend spleen focus-forming provirus: gp55 is an envelope fusion glycoprotein."
      Clark S.P., Mak T.W.
      Proc. Natl. Acad. Sci. U.S.A. 80:5037-5041(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "A deletion in the Friend spleen focus-forming virus env gene is necessary for its product (gp55) to be leukemogenic."
      Watanabe N., Nishi M., Ikawa Y., Amanuma H.
      J. Virol. 64:2678-2686(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    3. Erratum
      Watanabe N., Nishi M., Ikawa Y., Amanuma H.
      J. Virol. 64:5694-5694(1990)
    4. "Friend erythroleukemia revisited."
      Ney P.A., D'Andrea A.D.
      Blood 96:3675-3680(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.
    5. "The envelope glycoprotein of friend spleen focus-forming virus covalently interacts with and constitutively activates a truncated form of the receptor tyrosine kinase Stk."
      Nishigaki K., Thompson D., Hanson C., Yugawa T., Ruscetti S.
      J. Virol. 75:7893-7903(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MOUSE MST1R ISOFORM SF-STK.
    6. "The erythropoietin receptor transmembrane domain mediates complex formation with viral anemic and polycythemic gp55 proteins."
      Constantinescu S.N., Keren T., Russ W.P., Ubarretxena-Belandia I., Malka Y., Kubatzky K.F., Engelman D.M., Lodish H.F., Henis Y.I.
      J. Biol. Chem. 278:43755-43763(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, INTERACTION WITH MOUSE EPOR.

    Entry informationi

    Entry nameiENV_FRSF5
    AccessioniPrimary (citable) accession number: P03393
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Compared to other gammaretroviruses which possess 2 envelope proteins (gp70 and p15E), gp55 corresponds to a gp70-p15E fusion protein with a deletion of a portion of p15E. It is encoded by the defective env gene of the virus.
    The Friend murine leukemia virus complex induces a rapid and fatal erythroleukemia in adult mice. It is the replication-defective spleen focus-forming virus (SFFV) contained in this complex that causes foci of proliferating erythroid cells in spleens of infected mice. The second component is a replication competent Friend murine leukemia virus (F-MuLV) that serves as a helper virus for SFFV.

    External Data

    Dasty 3