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P03393

- ENV_FRSF5

UniProt

P03393 - ENV_FRSF5

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Protein

Glycoprotein 55

Gene

env

Organism
Friend spleen focus-forming virus (isolate 502) (FSFFV)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This envelope-like membrane glycoprotein is responsible for ligand-independent activation of the erythropoietin receptor EPOR leading to the abnormally rapid proliferation of erythroid precursor cells. In the first stage of Friend disease, constitutive activation of EPOR by gp55 causes uncontrolled, polyclonal proliferation of infected erythroblasts, leading to polycythemia (massive increase in the number of mature red cells). Host susceptibility to SSFV-induced erythroblastosis depends on the expression of the truncated isoform of MST1R receptor tyrosine kinase (MST1R isoform sf-Stk). Interaction with SSFV gp 55 results in constitutive tyrosine phosphorylation and activation of MST1R isoform sf-Stk.2 Publications

GO - Molecular functioni

  1. structural molecule activity Source: InterPro

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host cell membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Glycoprotein 55
Short name:
gp55
Gene namesi
Name:env
OrganismiFriend spleen focus-forming virus (isolate 502) (FSFFV)
Taxonomic identifieri355329 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
Virus hostiMus [TaxID: 10088]

Subcellular locationi

Host endoplasmic reticulum membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. Virion membrane Curated; Single-pass type I membrane protein Curated
Note: The envelope-like membrane glycoprotein gp55 is defective in its transport to the cell surface and remains associated predominantly with the rough endoplasmic reticulum (RER) membrane. It is almost not incorporated into virions. Host cell surface expression appears to be a prerequisite for its leukemogenicity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini33 – 384352Virion surfaceSequence AnalysisAdd
BLAST
Transmembranei385 – 40521HelicalSequence AnalysisAdd
BLAST
Topological domaini406 – 4094IntravirionSequence Analysis

GO - Cellular componenti

  1. host cell endoplasmic reticulum Source: UniProtKB-KW
  2. host cell plasma membrane Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
  4. viral capsid Source: InterPro
  5. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host endoplasmic reticulum, Host membrane, Membrane, Viral envelope protein, Virion

Pathology & Biotechi

Keywords - Diseasei

Oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence AnalysisAdd
BLAST
Chaini33 – 409377Glycoprotein 55PRO_0000040718Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi43 – 431N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi58 – 581N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi296 – 2961N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi328 – 3281N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi377 – 3771N-linked (GlcNAc...); by hostSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

PTM databases

UniCarbKBiP03393.

Interactioni

Subunit structurei

Homooligomer (Probable). Forms heterooligomers with mouse EPOR, probably via their respective transmembrane domains. Forms covalent heterodimers with mouse MST1R isoform sf-Stk, probably via disulfide bonds.1 PublicationCurated

Structurei

3D structure databases

ProteinModelPortaliP03393.
SMRiP03393. Positions 38-229.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi229 – 27850Pro-richAdd
BLAST

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.90.310.10. 1 hit.
InterProiIPR008981. FMuLV_rcpt-bd.
IPR018154. TLV/ENV_coat_polyprotein.
[Graphical view]
PANTHERiPTHR10424. PTHR10424. 1 hit.
PfamiPF00429. TLV_coat. 3 hits.
[Graphical view]
SUPFAMiSSF49830. SSF49830. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03393-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MKGPAFSKPL KDKINPWGPL IVLGILIRAG VSVQHDSPHQ VFNVTWRVTN
60 70 80 90 100
LMTGQTANAT SLLGTMTDAF PMLHFDLCDL IGDDWDETGL ECRTPGGRKR
110 120 130 140 150
ARTFDFYVCP GHTVPTGCGG PREGYCGKWG CETTGQAYWK PSSSWDLISL
160 170 180 190 200
KRGNTPKDRG PCYDSSVSSG VQGATPGGRC NPLVLKFTDA GKKASWDSPK
210 220 230 240 250
VWGLRLYRPT GIDPVTRFSL TRQVLNIGPR IPIGPNPVII GQLPPSRPVQ
260 270 280 290 300
VRLPRPPQPP PTGAASMVPG TAPPSQQPGT GDRLLNLVQG AYQALNLTNP
310 320 330 340 350
DKTQECWLCL VSGPPYYEGV AVLGTNSNHT SALKEKCCFY ADHTGLVRDS
360 370 380 390 400
MAKLRKRLTQ RQKLFESSQG WFEGSFNRSP WFTTLISTIM GLLIILLLLL

ILLLWTLHS
Length:409
Mass (Da):44,777
Last modified:July 21, 1986 - v1
Checksum:i407F56F769863D74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00021 Genomic RNA. Translation: AAA46487.1.
PIRiA03993. VCVW5S.
RefSeqiNP_041218.1. NC_001500.1.

Genome annotation databases

GeneIDi1491889.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00021 Genomic RNA. Translation: AAA46487.1 .
PIRi A03993. VCVW5S.
RefSeqi NP_041218.1. NC_001500.1.

3D structure databases

ProteinModelPortali P03393.
SMRi P03393. Positions 38-229.
ModBasei Search...
MobiDBi Search...

PTM databases

UniCarbKBi P03393.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1491889.

Family and domain databases

Gene3Di 3.90.310.10. 1 hit.
InterProi IPR008981. FMuLV_rcpt-bd.
IPR018154. TLV/ENV_coat_polyprotein.
[Graphical view ]
PANTHERi PTHR10424. PTHR10424. 1 hit.
Pfami PF00429. TLV_coat. 3 hits.
[Graphical view ]
SUPFAMi SSF49830. SSF49830. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence of an infectious clone of Friend spleen focus-forming provirus: gp55 is an envelope fusion glycoprotein."
    Clark S.P., Mak T.W.
    Proc. Natl. Acad. Sci. U.S.A. 80:5037-5041(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "A deletion in the Friend spleen focus-forming virus env gene is necessary for its product (gp55) to be leukemogenic."
    Watanabe N., Nishi M., Ikawa Y., Amanuma H.
    J. Virol. 64:2678-2686(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  3. Erratum
    Watanabe N., Nishi M., Ikawa Y., Amanuma H.
    J. Virol. 64:5694-5694(1990)
  4. "Friend erythroleukemia revisited."
    Ney P.A., D'Andrea A.D.
    Blood 96:3675-3680(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  5. "The envelope glycoprotein of friend spleen focus-forming virus covalently interacts with and constitutively activates a truncated form of the receptor tyrosine kinase Stk."
    Nishigaki K., Thompson D., Hanson C., Yugawa T., Ruscetti S.
    J. Virol. 75:7893-7903(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MOUSE MST1R ISOFORM SF-STK.
  6. "The erythropoietin receptor transmembrane domain mediates complex formation with viral anemic and polycythemic gp55 proteins."
    Constantinescu S.N., Keren T., Russ W.P., Ubarretxena-Belandia I., Malka Y., Kubatzky K.F., Engelman D.M., Lodish H.F., Henis Y.I.
    J. Biol. Chem. 278:43755-43763(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, INTERACTION WITH MOUSE EPOR.

Entry informationi

Entry nameiENV_FRSF5
AccessioniPrimary (citable) accession number: P03393
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Compared to other gammaretroviruses which possess 2 envelope proteins (gp70 and p15E), gp55 corresponds to a gp70-p15E fusion protein with a deletion of a portion of p15E. It is encoded by the defective env gene of the virus.
The Friend murine leukemia virus complex induces a rapid and fatal erythroleukemia in adult mice. It is the replication-defective spleen focus-forming virus (SFFV) contained in this complex that causes foci of proliferating erythroid cells in spleens of infected mice. The second component is a replication competent Friend murine leukemia virus (F-MuLV) that serves as a helper virus for SFFV.

External Data

Dasty 3