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P03389 (ENV_RSFFV) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glycoprotein 55

Short name=gp55
Gene names
Name:env
OrganismRauscher spleen focus-forming virus (RSFFV)
Taxonomic identifier11821 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
Virus hostMus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length408 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Envelope-like membrane glycoprotein.

Subcellular location

Host endoplasmic reticulum membrane; Single-pass type I membrane protein. Host cell membrane; Single-pass type I membrane protein. Virion membrane; Single-pass type I membrane protein Probable. Note: The envelope-like membrane glycoprotein gp55 is defective in its transport to the cell surface and remains associated predominantly with the rough endoplasmic reticulum (RER) membrane. It is almost not incorporated into virions. Host cell surface expression appears to be a prerequisite for its leukemogenicity By similarity.

Miscellaneous

Compared to other gammaretroviruses which possess 2 envelope proteins (gp70 and p15E), RSFFV gp55 corresponds to a gp70-p15E fusion protein with a deletion of a portion of p15E. It is encoded by the defective env gene of the virus.

The Rauscher murine leukemia virus complex induces a rapid and fatal erythroleukemia in adult mice. It is the replication-defective spleen focus-forming virus (SFFV) contained in this complex that causes foci of proliferating erythroid cells in spleens of infected mice. The second component is a replication competent Rauscher murine leukemia virus (R-MuLV) that serves as a helper virus for SFFV. Spleens of infected mice also contain a third component, the Rauscher mink cell focus-inducing (R-MCF) virus.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential
Chain33 – 408376Glycoprotein 55
PRO_0000040795

Regions

Topological domain33 – 385353Virion surface Potential
Transmembrane386 – 40621Helical; Potential
Topological domain407 – 4082Intravirion Potential
Coiled coil335 – 37137 Potential
Compositional bias230 – 27950Pro-rich

Amino acid modifications

Glycosylation431N-linked (GlcNAc...); by host Potential
Glycosylation581N-linked (GlcNAc...); by host Potential
Glycosylation2971N-linked (GlcNAc...); by host Potential
Glycosylation3291N-linked (GlcNAc...); by host Potential
Glycosylation3781N-linked (GlcNAc...); by host Potential

Sequences

Sequence LengthMass (Da)Tools
P03389 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 66383F99BCE9514A

FASTA40844,940
        10         20         30         40         50         60 
MEGPAFSKPL KDKINPWGPL IILGILIRAG VSVQHDSPHQ VFNVTWRVTN LMTGQTANAT 

        70         80         90        100        110        120 
SLLGTMTDAF PKLYFDLCDL IGDDWDETGL GCRTPGGRKR ARTFDFYVCP GHTVPTGCGG 

       130        140        150        160        170        180 
PREGYCGKWG CETTGQAYWK PSSSWDLISL KRGNTPRNQG PCYDSSAVSS DIKGATPGGR 

       190        200        210        220        230        240 
CNPLVLEFTD AGKKASWDGP KVWGLRLYRS TGTDPVTRFS LTRQVLNIGP RVPIGPNPVI 

       250        260        270        280        290        300 
TDQLPPSRPV QIMLPRPPQP PPPGAASIVP ETAPPSQQPG TGDRLLNLVD GAYQALNLTN 

       310        320        330        340        350        360 
PDKTQDCWLC LVSGPPYYEG VAVLGTYYNH TSALKEECCF YADHTGLVRD SMAKLRERLT 

       370        380        390        400 
QRQKLFESSQ GWFEELFNRS TWFTTLIFTI IGPLIILLLI LLFWTLHS 

« Hide

References

[1]"Molecular cloning of biologically active Rauscher spleen focus-forming virus and the sequences of its env gene and long terminal repeat."
Bestwick R.K., Boswell B.A., Kabat D.
J. Virol. 51:695-705(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K02375 Genomic RNA. Translation: AAA46505.1.
PIRVCMVSR. A03988.

3D structure databases

ProteinModelPortalP03389.
SMRP03389. Positions 38-230.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.90.310.10. 1 hit.
InterProIPR008981. FMuLV_rcpt-bd.
IPR018154. TLV/ENV_coat_polyprotein.
[Graphical view]
PANTHERPTHR10424. PTHR10424. 1 hit.
PfamPF00429. TLV_coat. 2 hits.
[Graphical view]
SUPFAMSSF49830. SSF49830. 1 hit.
ProtoNetSearch...

Entry information

Entry nameENV_RSFFV
AccessionPrimary (citable) accession number: P03389
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: February 19, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program