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P03388

- ENV_MCFF3

UniProt

P03388 - ENV_MCFF3

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Protein

Envelope glycoprotein

Gene

env

Organism
Mink cell focus-forming murine leukemia virus (isolate CI-3)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

The surface protein (SU) attaches the virus to the host cell by binding to its receptor. This interaction triggers the refolding of the transmembrane protein (TM) and is thought to activate its fusogenic potential by unmasking its fusion peptide. Fusion occurs at the host cell plasma membrane (By similarity).By similarity
The transmembrane protein (TM) acts as a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. Membranes fusion leads to delivery of the nucleocapsid into the cytoplasm (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei441 – 4422Cleavage; by hostBy similarity
Sitei621 – 6222Cleavage; by viral protease p14By similarity

GO - Molecular functioni

  1. structural molecule activity Source: InterPro

GO - Biological processi

  1. fusion of virus membrane with host plasma membrane Source: UniProtKB-KW
  2. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host cell membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein
Alternative name(s):
Env polyprotein
Cleaved into the following 3 chains:
Surface protein
Short name:
SU
Alternative name(s):
Glycoprotein 70
Short name:
gp70
Transmembrane protein
Short name:
TM
Alternative name(s):
Envelope protein p15E
Alternative name(s):
p2E
Gene namesi
Name:env
OrganismiMink cell focus-forming murine leukemia virus (isolate CI-3)
Taxonomic identifieri11936 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostiMus musculus (Mouse) [TaxID: 10090]

Subcellular locationi

Chain Surface protein : Virion membrane; Peripheral membrane protein. Host cell membrane By similarity; Peripheral membrane protein By similarity
Note: The surface protein is not anchored to the viral envelope, but associates with the virion surface through its binding to TM. Both proteins are thought to be concentrated at the site of budding and incorporated into the virions possibly by contacts between the cytoplasmic tail of Env and the N-terminus of Gag (By similarity).By similarity
R-peptide : Host cell membrane By similarity; Peripheral membrane protein By similarity
Note: The R-peptide is membrane-associated through its palmitate.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini33 – 582550ExtracellularSequence AnalysisAdd
BLAST
Transmembranei583 – 60321HelicalSequence AnalysisAdd
BLAST
Topological domaini604 – 64037CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
  3. viral capsid Source: InterPro
  4. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence AnalysisAdd
BLAST
Chaini33 – 640608Envelope glycoproteinPRO_0000239578Add
BLAST
Chaini33 – 441409Surface proteinBy similarityPRO_0000040742Add
BLAST
Chaini442 – 621180Transmembrane proteinBy similarityPRO_0000040743Add
BLAST
Peptidei622 – 64019R-peptideBy similarityPRO_0000040744Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi43 – 431N-linked (GlcNAc...); by hostBy similarity
Glycosylationi58 – 581N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi109 ↔ 126By similarity
Disulfide bondi118 ↔ 131By similarity
Glycosylationi297 – 2971N-linked (GlcNAc...); by hostBy similarity
Disulfide bondi307 ↔ 535Interchain (between SU and TM chains, or C-310 with C-535); in linked formBy similarity
Disulfide bondi307 ↔ 310By similarity
Glycosylationi329 – 3291N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi336 – 3361N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi337 ↔ 391By similarity
Disulfide bondi356 ↔ 368By similarity
Glycosylationi369 – 3691N-linked (GlcNAc...); by hostSequence Analysis
Disulfide bondi398 ↔ 411By similarity
Disulfide bondi527 ↔ 534By similarity

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins. Envelope glycoproteins are synthesized as a inactive precursor that is N-glycosylated and processed likely by host cell furin or by a furin-like protease in the Golgi to yield the mature SU and TM proteins. The cleavage site between SU and TM requires the minimal sequence [KR]-X-[KR]-R. The R-peptide is released from the C-terminus of the cytoplasmic tail of the TM protein upon particle formation as a result of proteolytic cleavage by the viral protease. Cleavage of this peptide is required for TM to become fusogenic (By similarity).By similarity
The CXXC motif is highly conserved across a broad range of retroviral envelope proteins. It is thought to participate in the formation of a labile disulfide bond possibly with the CX6CC motif present in the transmembrane protein. Isomerization of the intersubunit disulfide bond to an SU intrachain disulfide bond is thought to occur upon receptor recognition in order to allow membrane fusion (By similarity).By similarity
The R-peptide is palmitoylated.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Interactioni

Subunit structurei

The mature envelope protein (Env) consists of a trimer of SU-TM heterodimers attached by a labile interchain disulfide bond.By similarity

Structurei

3D structure databases

ProteinModelPortaliP03388.
SMRiP03388. Positions 38-230, 487-539.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni444 – 46421Fusion peptideBy similarityAdd
BLAST
Regioni510 – 52617ImmunosuppressionBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili473 – 50937Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi307 – 3104CXXC
Motifi527 – 5359CX6CC
Motifi627 – 6304YXXL motif; contains endocytosis signalBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi255 – 2639Poly-Pro

Domaini

The 17 amino acids long immunosuppressive region is present in many retroviral envelope proteins. Synthetic peptides derived from this relatively conserved sequence inhibit immune function in vitro and in vivo (By similarity).By similarity
The YXXL motif is involved in determining the exact site of viral release at the surface of infected mononuclear cells and promotes endocytosis.

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.90.310.10. 1 hit.
InterProiIPR008981. FMuLV_rcpt-bd.
IPR018154. TLV/ENV_coat_polyprotein.
[Graphical view]
PANTHERiPTHR10424. PTHR10424. 1 hit.
PfamiPF00429. TLV_coat. 1 hit.
[Graphical view]
SUPFAMiSSF49830. SSF49830. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03388-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEGPAFSKPL KDKINPWGPL IILGILIRAG VSVQHDSPHQ VFNVTWRVTN
60 70 80 90 100
LMTGQTANAT SLLGTMTDAF PKLYFDLCDL VGDDWDETGL GCRTPGGRKR
110 120 130 140 150
ARTFDFYVCP GHTVPTGCGG PREGYCGKWG CETTGQAYWK PSSSWDLISL
160 170 180 190 200
KRGNTPRNQG PCYDSSAVSS DIKGATPGGR CNPLVLEFTD AGKKASWDGP
210 220 230 240 250
KVWGLRLYRS TGTDPVTRFS LTRQVLNIGP RVPIGPNPVI TDQLPPSRPV
260 270 280 290 300
QIMLPRPPQP PPPGAASIVP ETAPPSQQLG TGDRLLNLVN GAYQALNLTS
310 320 330 340 350
PDKTQECWLC LVAGPPYYEG VAVLGTYSNH TSAPANCSVA SQHKLTLSGV
360 370 380 390 400
AGRGLCIAAF PKTHQALCNT TQKTSDGSYH LAAPAGTIWA CNTGLTPCLS
410 420 430 440 450
TTVLDLTTDY CVLVELWPKV TYHSPSYVYG QFEKKKTKYK REPVSLTLAL
460 470 480 490 500
LLGGLTMGGI AAGVGTGTTA LVATQQFQQL QAAMHDDLKE VEKSITNLEK
510 520 530 540 550
SLTSLSEVVL QNRRGLDLLF LKEGGLCAAL KEECCFYADH TGLVRDSMAK
560 570 580 590 600
LRERLSQRQK LFESQQGWFE GLFNKSPWFT TLISTIMGPL IILLLILLFG
610 620 630 640
PWILNRLVQF IKDRISVVQA LVLTQQYHQL KTIGDCKSRE
Length:640
Mass (Da):69,726
Last modified:July 21, 1986 - v1
Checksum:iC2C71A6749128CF5
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti315 – 542228Missing in strain: Isolate CI-4.
Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02725 Genomic RNA. Translation: AAA46375.1.
K02726 Genomic RNA. Translation: AAA46376.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02725 Genomic RNA. Translation: AAA46375.1 .
K02726 Genomic RNA. Translation: AAA46376.1 .

3D structure databases

ProteinModelPortali P03388.
SMRi P03388. Positions 38-230, 487-539.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.90.310.10. 1 hit.
InterProi IPR008981. FMuLV_rcpt-bd.
IPR018154. TLV/ENV_coat_polyprotein.
[Graphical view ]
PANTHERi PTHR10424. PTHR10424. 1 hit.
Pfami PF00429. TLV_coat. 1 hit.
[Graphical view ]
SUPFAMi SSF49830. SSF49830. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Envelope gene sequence of two in vitro-generated mink cell focus-forming murine leukemia viruses which contain the entire gp70 sequence of the endogenous nonecotropic parent."
    Mark G.E., Rapp U.R.
    J. Virol. 49:530-539(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    Strain: Isolate CI-3 and Isolate CI-4.

Entry informationi

Entry nameiENV_MCFF3
AccessioniPrimary (citable) accession number: P03388
Secondary accession number(s): Q85501
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

The sequence shown is that of isolate CI-3.

External Data

Dasty 3