Reviewed, UniProtKB/Swiss-Prot P03371 (POL_EIAVY)

Names and origin

Protein names

Recommended name:
    Pol polyprotein
Cleaved into the following 3 chains:
    1- Recommended name:
            Protease
        Alternative name(s):
            Retropepsin
            EC=3.4.23.-
    2- Recommended name:
            Reverse transcriptase/ribonuclease H
                Short name=RT
              EC=2.7.7.49
              EC=3.1.26.4
    3- Recommended name:
            Integrase
                Short name=IN

Gene names

Name:

pol

Organism

Equine infectious anemia virus (strain Wyoming) (EIAV)

Taxonomic identifier

11672 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Lentivirus › Equine lentivirus group

Virus host

Equus asinus (Donkey) [TaxID: 9793]
Equus caballus (Horse) [TaxID: 9796]

Protein attributes

Sequence length	1145 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins.
Sequence similarities	Belongs to the retroviral Pol polyprotein family. Contains 1 integrase catalytic domain. Contains 1 integrase-type DNA-binding domain. Contains 1 integrase-type zinc finger. Contains 1 peptidase A2 domain. Contains 1 reverse transcriptase domain. Contains 1 RNase H domain.
Caution	The original EMBL accession numbers (M11337 and M14855) assigned to this isolate (isolate Wyoming) have been made secondary to M16575 which is from a different isolate (clone 1365).

Ontologies

Keywords
Biological process	DNA integration DNA recombination
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Aspartyl protease Endonuclease Hydrolase Nuclease Nucleotidyltransferase Protease RNA-directed DNA polymerase Transferase
PTM	Cleavage on pair of basic residues
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA integration Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro dUTP metabolic process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro transposition Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW integrase activity Inferred from electronic annotation. Source: InterPro ribonuclease H activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 195	195	Protease		PRO_0000038835
	Chain	196 – 913	718	Reverse transcriptase/ribonuclease H		PRO_0000038836
	Chain	914 – 1145	232	Integrase		PRO_0000038837
Regions
	Domain	100 – 174	75	Peptidase A2
	Domain	230 – 418	189	Reverse transcriptase
	Domain	616 – 739	124	RNase H
	Domain	920 – 1077	158	Integrase catalytic
	Zinc finger	877 – 918	42	Integrase-type
	DNA binding	1095 – 1143	49	Integrase-type
Sites
	Active site	105	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P03371-1 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: EE723380950D5D9E	FASTA	1,145	129,520
	10 20 30 40 50 60 TAWTFLKAMQ KCSKKREARG SREAPETNFP DTTEESAQQI CCTRDSSDSK SVPRSERNKK 70 80 90 100 110 120 GIQCQGEGSS RGSQPGQFVG VTYNLEKRPT TIVLINDTPL NVLLDTGADT SVLTTAHYNR 130 140 150 160 170 180 LKYRGRKYQG TGIIGVGGNV ETFSTPVTIK KKGRHIKTRM LVADIPVTIL GRDILQDLGA 190 200 210 220 230 240 KLVLAQLSKE IKFRKIELKE GTMGPKIPQW PLTKEKLEGA KETVQRLLSE GKISEASDNN 250 260 270 280 290 300 PYNSPIFVIK KRSGKWRLLQ DLRELNKTVQ VGTEISRGLP HPGGLIKCKH MTVLDIGDAY 310 320 330 340 350 360 FTIPLDPEFR PYTAFTIPSI NHQEPDKRYV WKCLPQGFVL SPYIYQKTLQ EILQPFRERY 370 380 390 400 410 420 PEVQLYQYMD DLFVGSNGSK KQHKELIIEL RAILQKGFET PDDKLQEVPP YSWLGYQLCP 430 440 450 460 470 480 ENWKVQKMQL DMVKNPTLND VQKLMGNITW MSSGVPGLTV KHIAATTKGC LELNQKVIWT 490 500 510 520 530 540 EEAQKELEEN NEKIKNAQGL QYYNPEEEML CEVEITKNYE ATYVIKQSQG ILWAGKKIMK 550 560 570 580 590 600 ANKGWSTVKN LMLLLQHVAT ESITRVGKCP TFKVPFTKEQ VMWEMQKGWY YSWLPEIVYT 610 620 630 640 650 660 HQVVHDDWRM KLVEEPTSGI TIYTDGGKQN GEGIAAYVTS NGRTKQKRLG PVTHQVAERM 670 680 690 700 710 720 AIQMALEDTR DKQVNIVTDS YYCWKNITEG LGLEGPQNPW WPIIQNIREK EIVYFAWVPG 730 740 750 760 770 780 HKGIYGNQLA DEAAKIKEEI MLAYQGTQIK EKRDEDAGFD LCVPYDIMIP VSDTKIIPTD 790 800 810 820 830 840 VKIQVPPNSF GWVTGKSSMA KQGLLINGGI IDEGYTGEIQ VICTNIGKSN IKLIEGQKFA 850 860 870 880 890 900 QLIILQHHSN SRQPWDENKI SQRGDKGFGS TGVFWVENIQ EAQDEHENWH TSPKILARNY 910 920 930 940 950 960 KIPLTVAKQI TQECPHCTKQ GSGPAGCVMR SPNHWQADCT HLDNKIILHF VESNSGYIHA 970 980 990 1000 1010 1020 TLLSKENALC TSLAILEWAR LFSPKSLHTD NGTNFVAEPV VNLLKFLKIA HTTGIPYHPE 1030 1040 1050 1060 1070 1080 SQGIVERANR TLKEKIQSHR DNTQTLEAAL QLALITCNKG RESMGGQTPW EVFITNQAQV 1090 1100 1110 1120 1130 1140 IHEKLLLQQA QSSKKFCFYK IPGEHDWKGP TRVLWKGDGA VVVNDEGKGI IAVPLTRTKL LIKPN

References

[1]	"Equine infectious anemia virus gag and pol genes: relatedness to visna and AIDS virus." Stephens R.M., Casey J.W., Rice N.R. Science 231:589-594(1986) [PubMed: 3003905] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Lentivirus genomic organization: the complete nucleotide sequence of the env gene region of equine infectious anemia virus." Rushlow K., Olsen K., Stiegler G., Payne S.L., Montelaro R.C., Issel C.J. Virology 155:309-321(1986) [PubMed: 2431539] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 1029-1145.

Cross-references

Sequence databases
PIR	GNLJEV. A03970.
3D structure databases
HSSP	HSSP built from PDB template 1DUN based on UniProtKB P11204.
SMR	P03371. Positions 81-184, 741-860.
ModBase	Search...
Family and domain databases
InterPro	IPR008180. DeoxyUTPase. IPR000477. DNA_pol_RVTase. IPR001037. Integrase_C_retrovir. IPR001584. Integrase_cat-core. IPR017856. Integrase_Zn-bd_dom-like_N. IPR003308. Integrase_Zn-bd_dom_N. IPR018061. Pept_A2A_retrovirus_sg. IPR001995. Peptidase_A2_cat. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. IPR002156. RNase_H. IPR010659. RVT_connect. IPR010661. RVT_thumb. [Graphical view]
Gene3D	G3DSA:2.30.30.10. Integrase_C. 1 hit. G3DSA:1.10.10.200. Intgrase_N_Zn_bd. 1 hit. G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. PF00552. Integrase. 1 hit. PF02022. Integrase_Zn. 1 hit. PF00075. RnaseH. 1 hit. PF00665. rve. 1 hit. PF00077. RVP. 1 hit. PF00078. RVT_1. 1 hit. PF06815. RVT_connect. 1 hit. PF06817. RVT_thumb. 1 hit. [Graphical view]
ProDom	PD004900. dCTP_deaminase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS50175. ASP_PROT_RETROV. 1 hit. PS00141. ASP_PROTEASE. 1 hit. PS50994. INTEGRASE. 1 hit. PS51027. INTEGRASE_DBD. 1 hit. PS50879. RNASE_H. 1 hit. PS50878. RT_POL. 1 hit. PS50876. ZF_INTEGRASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POL_EIAVY

Accession

Primary (citable) accession number: P03371

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	June 16, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)