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P03356

- POL_MLVAV

UniProt

P03356 - POL_MLVAV

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Protein

Pol polyprotein

Gene

pol

Organism
AKV murine leukemia virus (AKR (endogenous) murine leukemia virus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.PROSITE-ProRule annotation
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei27 – 271PROSITE-ProRule annotation

GO - Molecular functioni

  1. aspartic-type endopeptidase activity Source: UniProtKB-KW
  2. nucleic acid binding Source: InterPro
  3. RNA-directed DNA polymerase activity Source: UniProtKB-KW
  4. RNA-DNA hybrid ribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA integration Source: UniProtKB-KW
  2. DNA recombination Source: UniProtKB-KW
  3. establishment of integrated proviral latency Source: UniProtKB-KW
  4. viral entry into host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

Keywords - Biological processi

DNA integration, DNA recombination, Viral genome integration, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Pol polyprotein
Cleaved into the following 3 chains:
Gene namesi
Name:pol
OrganismiAKV murine leukemia virus (AKR (endogenous) murine leukemia virus)
Taxonomic identifieri11791 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
ProteomesiUP000008875: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 1196IntegrasePRO_0000259724
Chaini1 – 11961196Pol polyproteinPRO_0000259722Add
BLAST
Chaini1 – 103103ProteasePRO_0000026129Add
BLAST
Chaini104 – ?Reverse transcriptase/ribonuclease HPRO_0000259723

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins.

Structurei

3D structure databases

ProteinModelPortaliP03356.
SMRiP03356. Positions 144-594, 623-788.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 9271Peptidase A2PROSITE-ProRule annotationAdd
BLAST
Domaini202 – 393192Reverse transcriptasePROSITE-ProRule annotationAdd
BLAST
Domaini635 – 781147RNase HPROSITE-ProRule annotationAdd
BLAST
Domaini905 – 1063159Integrase catalyticPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the retroviral Pol polyprotein family.Curated
Contains 1 integrase catalytic domain.PROSITE-ProRule annotation
Contains 1 peptidase A2 domain.PROSITE-ProRule annotation
Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation
Contains 1 RNase H domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.40.70.10. 1 hit.
3.30.420.10. 2 hits.
InterProiIPR001969. Aspartic_peptidase_AS.
IPR001584. Integrase_cat-core.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view]
SUPFAMiSSF50630. SSF50630. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEiPS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03356-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
GGQGQEPPPE PRITLTVGGQ PVTFLVDTGA QHSVLTQNPG PLSDRSAWVQ
60 70 80 90 100
GATGGKRYRW TTDRKVHLAT GKVTHSFLHV PDCPYPLLGR DLLTKLKAQI
110 120 130 140 150
HFEGSGAQVV GPKGQPLQVL TLNLEDEYRL YETSAEPEVS PGSTWLSDFP
160 170 180 190 200
QAWAETGGMG LAVRQAPLII PLKATSTPVS IKQYPMSQEA KLGIKPHIQR
210 220 230 240 250
LLDQGILVPC QSPWNTPLLP VKKPGTNDYR PVQDLREVNK RVEDIHPTVP
260 270 280 290 300
NPYNLLSGLP PSHRWYTVLD LKDAFFCLRL HPTSQPLFAF EWRDPGMGIS
310 320 330 340 350
GQLTWTRLPQ GFKNSPTLFD EALHRDLADF RIQHPDLILL QYVDDILLAA
360 370 380 390 400
TSELDCQQGT RALLLTLGNL GYRASAKKAQ LCQKQVKYLG YLLKEGQRWL
410 420 430 440 450
TEARKETVMG QPTPKTPRQL REFLGTAGFC RLWIPGFAEM AAPLYPLTKT
460 470 480 490 500
GTLFNWGPDQ QKAYQEIKQA LLTAPALGLP DLTKPFELFV DEKQGYAKGV
510 520 530 540 550
LTQKLGPWRR PVAYLSKKLD PVAAGWPPCL RMVAAIAVLR KDAGKLTMGQ
560 570 580 590 600
PLVILAPHAV EALVKQPPDR WLSNARMTHY QAMLLDTDRV QFGPVVALNP
610 620 630 640 650
ATLLPLPEEG APHDCLEILA ETHGTRPDLT DQPIPDADHT WYTDGSSFLQ
660 670 680 690 700
EGQRKAGAAV TTETEVIWAR ALPAGTSAQR AELIALTQAL KMAEGKRLNV
710 720 730 740 750
YTDSRYAFAT AHIHGEIYRR RGLLTSEGRE IKNKSEILAL LKALFLPKRL
760 770 780 790 800
SIIHCLGHQK GDSAEARGNR LADQAAREAA IKTPPDTSTL LIEDSTPYTP
810 820 830 840 850
AYFHYTETDL KKLRELGATY NQSKGYWVFQ GKPVMPDQFV FELLDSLHRL
860 870 880 890 900
THLGYQKMKA LLDRGESPYY MLNRDKTLQY VADSCTVCAQ VNASKAKIGA
910 920 930 940 950
GVRVRGHRPG SHWEIDFTEV KPGLYGYKYL LVFVDTFSGW VEAFPTKRET
960 970 980 990 1000
ARVVSKKLLE EIFPRFGMPQ VLGSDNGPAF TSQVSQSVAD LLGIDWKLHC
1010 1020 1030 1040 1050
AYRPQSSGQV ERMNRTIKET LTKLTLAAGT RDWVLLLPLA LYRARNTPGP
1060 1070 1080 1090 1100
HGLTPYEILY GAPPPLVNFH DPDMSELTNS PSLQAHLQAL QTVQREIWKP
1110 1120 1130 1140 1150
LAEAYRDQLD QPVIPHPFRI GDSVWVRRHQ TKNLEPRWKG PYTVLLTTPT
1160 1170 1180 1190
ALKVDGISAW IHAAHVKAAT TPPIKPSWRV QRSQNPLKIR LTRGAP
Length:1,196
Mass (Da):133,413
Last modified:May 4, 2001 - v2
Checksum:iEDBAE9F550839A01
GO

Sequence cautioni

The sequence AAB03091.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti540 – 5401R → T(PubMed:6294621)Curated
Sequence conflicti996 – 9961Missing(PubMed:6294621)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01998 Genomic RNA. Translation: AAB03091.1. Different initiation.
PIRiA03958. GNVWK.

Keywords - Coding sequence diversityi

RNA suppression of termination

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01998 Genomic RNA. Translation: AAB03091.1 . Different initiation.
PIRi A03958. GNVWK.

3D structure databases

ProteinModelPortali P03356.
SMRi P03356. Positions 144-594, 623-788.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.40.70.10. 1 hit.
3.30.420.10. 2 hits.
InterProi IPR001969. Aspartic_peptidase_AS.
IPR001584. Integrase_cat-core.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
[Graphical view ]
Pfami PF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view ]
SUPFAMi SSF50630. SSF50630. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEi PS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of AKV murine leukemia virus."
    Herr W.
    J. Virol. 49:471-478(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 353-1196.

Entry informationi

Entry nameiPOL_MLVAV
AccessioniPrimary (citable) accession number: P03356
Secondary accession number(s): P03357
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: May 4, 2001
Last modified: October 29, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is synthesized as a Gag-Pol polyprotein.

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3