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P03356

- POL_MLVAV

UniProt

P03356 - POL_MLVAV

Protein

Pol polyprotein

Gene

pol

Organism
AKV murine leukemia virus (AKR (endogenous) murine leukemia virus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 2 (04 May 2001)
      Previous versions | rss
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    Functioni

    During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

    Catalytic activityi

    Endonucleolytic cleavage to 5'-phosphomonoester.PROSITE-ProRule annotation
    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei27 – 271PROSITE-ProRule annotation

    GO - Molecular functioni

    1. aspartic-type endopeptidase activity Source: UniProtKB-KW
    2. RNA binding Source: InterPro
    3. RNA-directed DNA polymerase activity Source: UniProtKB-KW
    4. RNA-DNA hybrid ribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA integration Source: UniProtKB-KW
    2. DNA recombination Source: UniProtKB-KW
    3. establishment of integrated proviral latency Source: UniProtKB-KW
    4. viral entry into host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Aspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

    Keywords - Biological processi

    DNA integration, DNA recombination, Viral genome integration, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pol polyprotein
    Cleaved into the following 3 chains:
    Gene namesi
    Name:pol
    OrganismiAKV murine leukemia virus (AKR (endogenous) murine leukemia virus)
    Taxonomic identifieri11791 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
    Virus hostiMus musculus (Mouse) [TaxID: 10090]
    ProteomesiUP000008875: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 1196IntegrasePRO_0000259724
    Chaini1 – 11961196Pol polyproteinPRO_0000259722Add
    BLAST
    Chaini1 – 103103ProteasePRO_0000026129Add
    BLAST
    Chaini104 – ?Reverse transcriptase/ribonuclease HPRO_0000259723

    Post-translational modificationi

    Specific enzymatic cleavages in vivo yield mature proteins.

    Structurei

    3D structure databases

    ProteinModelPortaliP03356.
    SMRiP03356. Positions 144-594, 623-788.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini22 – 9271Peptidase A2PROSITE-ProRule annotationAdd
    BLAST
    Domaini202 – 393192Reverse transcriptasePROSITE-ProRule annotationAdd
    BLAST
    Domaini635 – 781147RNase HPROSITE-ProRule annotationAdd
    BLAST
    Domaini905 – 1063159Integrase catalyticPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the retroviral Pol polyprotein family.Curated
    Contains 1 integrase catalytic domain.PROSITE-ProRule annotation
    Contains 1 peptidase A2 domain.PROSITE-ProRule annotation
    Contains 1 reverse transcriptase domain.PROSITE-ProRule annotation
    Contains 1 RNase H domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di2.40.70.10. 1 hit.
    3.30.420.10. 2 hits.
    InterProiIPR001969. Aspartic_peptidase_AS.
    IPR001584. Integrase_cat-core.
    IPR018061. Pept_A2A_retrovirus_sg.
    IPR001995. Peptidase_A2_cat.
    IPR021109. Peptidase_aspartic_dom.
    IPR012337. RNaseH-like_dom.
    IPR002156. RNaseH_domain.
    IPR000477. RT_dom.
    [Graphical view]
    PfamiPF00075. RNase_H. 1 hit.
    PF00665. rve. 1 hit.
    PF00077. RVP. 1 hit.
    PF00078. RVT_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50630. SSF50630. 1 hit.
    SSF53098. SSF53098. 2 hits.
    PROSITEiPS50175. ASP_PROT_RETROV. 1 hit.
    PS00141. ASP_PROTEASE. 1 hit.
    PS50994. INTEGRASE. 1 hit.
    PS50879. RNASE_H. 1 hit.
    PS50878. RT_POL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P03356-1 [UniParc]FASTAAdd to Basket

    « Hide

    GGQGQEPPPE PRITLTVGGQ PVTFLVDTGA QHSVLTQNPG PLSDRSAWVQ     50
    GATGGKRYRW TTDRKVHLAT GKVTHSFLHV PDCPYPLLGR DLLTKLKAQI 100
    HFEGSGAQVV GPKGQPLQVL TLNLEDEYRL YETSAEPEVS PGSTWLSDFP 150
    QAWAETGGMG LAVRQAPLII PLKATSTPVS IKQYPMSQEA KLGIKPHIQR 200
    LLDQGILVPC QSPWNTPLLP VKKPGTNDYR PVQDLREVNK RVEDIHPTVP 250
    NPYNLLSGLP PSHRWYTVLD LKDAFFCLRL HPTSQPLFAF EWRDPGMGIS 300
    GQLTWTRLPQ GFKNSPTLFD EALHRDLADF RIQHPDLILL QYVDDILLAA 350
    TSELDCQQGT RALLLTLGNL GYRASAKKAQ LCQKQVKYLG YLLKEGQRWL 400
    TEARKETVMG QPTPKTPRQL REFLGTAGFC RLWIPGFAEM AAPLYPLTKT 450
    GTLFNWGPDQ QKAYQEIKQA LLTAPALGLP DLTKPFELFV DEKQGYAKGV 500
    LTQKLGPWRR PVAYLSKKLD PVAAGWPPCL RMVAAIAVLR KDAGKLTMGQ 550
    PLVILAPHAV EALVKQPPDR WLSNARMTHY QAMLLDTDRV QFGPVVALNP 600
    ATLLPLPEEG APHDCLEILA ETHGTRPDLT DQPIPDADHT WYTDGSSFLQ 650
    EGQRKAGAAV TTETEVIWAR ALPAGTSAQR AELIALTQAL KMAEGKRLNV 700
    YTDSRYAFAT AHIHGEIYRR RGLLTSEGRE IKNKSEILAL LKALFLPKRL 750
    SIIHCLGHQK GDSAEARGNR LADQAAREAA IKTPPDTSTL LIEDSTPYTP 800
    AYFHYTETDL KKLRELGATY NQSKGYWVFQ GKPVMPDQFV FELLDSLHRL 850
    THLGYQKMKA LLDRGESPYY MLNRDKTLQY VADSCTVCAQ VNASKAKIGA 900
    GVRVRGHRPG SHWEIDFTEV KPGLYGYKYL LVFVDTFSGW VEAFPTKRET 950
    ARVVSKKLLE EIFPRFGMPQ VLGSDNGPAF TSQVSQSVAD LLGIDWKLHC 1000
    AYRPQSSGQV ERMNRTIKET LTKLTLAAGT RDWVLLLPLA LYRARNTPGP 1050
    HGLTPYEILY GAPPPLVNFH DPDMSELTNS PSLQAHLQAL QTVQREIWKP 1100
    LAEAYRDQLD QPVIPHPFRI GDSVWVRRHQ TKNLEPRWKG PYTVLLTTPT 1150
    ALKVDGISAW IHAAHVKAAT TPPIKPSWRV QRSQNPLKIR LTRGAP 1196
    Length:1,196
    Mass (Da):133,413
    Last modified:May 4, 2001 - v2
    Checksum:iEDBAE9F550839A01
    GO

    Sequence cautioni

    The sequence AAB03091.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti540 – 5401R → T(PubMed:6294621)Curated
    Sequence conflicti996 – 9961Missing(PubMed:6294621)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01998 Genomic RNA. Translation: AAB03091.1. Different initiation.
    PIRiA03958. GNVWK.

    Keywords - Coding sequence diversityi

    RNA suppression of termination

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01998 Genomic RNA. Translation: AAB03091.1 . Different initiation.
    PIRi A03958. GNVWK.

    3D structure databases

    ProteinModelPortali P03356.
    SMRi P03356. Positions 144-594, 623-788.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.40.70.10. 1 hit.
    3.30.420.10. 2 hits.
    InterProi IPR001969. Aspartic_peptidase_AS.
    IPR001584. Integrase_cat-core.
    IPR018061. Pept_A2A_retrovirus_sg.
    IPR001995. Peptidase_A2_cat.
    IPR021109. Peptidase_aspartic_dom.
    IPR012337. RNaseH-like_dom.
    IPR002156. RNaseH_domain.
    IPR000477. RT_dom.
    [Graphical view ]
    Pfami PF00075. RNase_H. 1 hit.
    PF00665. rve. 1 hit.
    PF00077. RVP. 1 hit.
    PF00078. RVT_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50630. SSF50630. 1 hit.
    SSF53098. SSF53098. 2 hits.
    PROSITEi PS50175. ASP_PROT_RETROV. 1 hit.
    PS00141. ASP_PROTEASE. 1 hit.
    PS50994. INTEGRASE. 1 hit.
    PS50879. RNASE_H. 1 hit.
    PS50878. RT_POL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of AKV murine leukemia virus."
      Herr W.
      J. Virol. 49:471-478(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 353-1196.

    Entry informationi

    Entry nameiPOL_MLVAV
    AccessioniPrimary (citable) accession number: P03356
    Secondary accession number(s): P03357
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: May 4, 2001
    Last modified: October 1, 2014
    This is version 108 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This protein is synthesized as a Gag-Pol polyprotein.

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3