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P03356

- POL_MLVAV

UniProt

P03356 - POL_MLVAV

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Protein
Pol polyprotein
Gene
pol
Organism
AKV murine leukemia virus (AKR (endogenous) murine leukemia virus)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei27 – 271 By similarity

GO - Molecular functioni

  1. RNA binding Source: InterPro
  2. RNA-DNA hybrid ribonuclease activity Source: UniProtKB-EC
  3. RNA-directed DNA polymerase activity Source: UniProtKB-KW
  4. aspartic-type endopeptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. DNA integration Source: UniProtKB-KW
  2. DNA recombination Source: UniProtKB-KW
  3. establishment of integrated proviral latency Source: UniProtKB-KW
  4. viral entry into host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Endonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Protease, RNA-directed DNA polymerase, Transferase

Keywords - Biological processi

DNA integration, DNA recombination, Viral genome integration, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Pol polyprotein
Cleaved into the following 3 chains:
Gene namesi
Name:pol
OrganismiAKV murine leukemia virus (AKR (endogenous) murine leukemia virus)
Taxonomic identifieri11791 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
ProteomesiUP000008875: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 1196IntegrasePRO_0000259724
Chaini1 – 11961196Pol polyprotein
PRO_0000259722Add
BLAST
Chaini1 – 103103Protease
PRO_0000026129Add
BLAST
Chaini104 – ?Reverse transcriptase/ribonuclease HPRO_0000259723

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins.

Structurei

3D structure databases

ProteinModelPortaliP03356.
SMRiP03356. Positions 144-594, 623-788.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 9271Peptidase A2
Add
BLAST
Domaini202 – 393192Reverse transcriptase
Add
BLAST
Domaini635 – 781147RNase H
Add
BLAST
Domaini905 – 1063159Integrase catalytic
Add
BLAST

Sequence similaritiesi

Contains 1 RNase H domain.

Family and domain databases

Gene3Di2.40.70.10. 1 hit.
3.30.420.10. 2 hits.
InterProiIPR001969. Aspartic_peptidase_AS.
IPR001584. Integrase_cat-core.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view]
SUPFAMiSSF50630. SSF50630. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEiPS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03356-1 [UniParc]FASTAAdd to Basket

« Hide

GGQGQEPPPE PRITLTVGGQ PVTFLVDTGA QHSVLTQNPG PLSDRSAWVQ     50
GATGGKRYRW TTDRKVHLAT GKVTHSFLHV PDCPYPLLGR DLLTKLKAQI 100
HFEGSGAQVV GPKGQPLQVL TLNLEDEYRL YETSAEPEVS PGSTWLSDFP 150
QAWAETGGMG LAVRQAPLII PLKATSTPVS IKQYPMSQEA KLGIKPHIQR 200
LLDQGILVPC QSPWNTPLLP VKKPGTNDYR PVQDLREVNK RVEDIHPTVP 250
NPYNLLSGLP PSHRWYTVLD LKDAFFCLRL HPTSQPLFAF EWRDPGMGIS 300
GQLTWTRLPQ GFKNSPTLFD EALHRDLADF RIQHPDLILL QYVDDILLAA 350
TSELDCQQGT RALLLTLGNL GYRASAKKAQ LCQKQVKYLG YLLKEGQRWL 400
TEARKETVMG QPTPKTPRQL REFLGTAGFC RLWIPGFAEM AAPLYPLTKT 450
GTLFNWGPDQ QKAYQEIKQA LLTAPALGLP DLTKPFELFV DEKQGYAKGV 500
LTQKLGPWRR PVAYLSKKLD PVAAGWPPCL RMVAAIAVLR KDAGKLTMGQ 550
PLVILAPHAV EALVKQPPDR WLSNARMTHY QAMLLDTDRV QFGPVVALNP 600
ATLLPLPEEG APHDCLEILA ETHGTRPDLT DQPIPDADHT WYTDGSSFLQ 650
EGQRKAGAAV TTETEVIWAR ALPAGTSAQR AELIALTQAL KMAEGKRLNV 700
YTDSRYAFAT AHIHGEIYRR RGLLTSEGRE IKNKSEILAL LKALFLPKRL 750
SIIHCLGHQK GDSAEARGNR LADQAAREAA IKTPPDTSTL LIEDSTPYTP 800
AYFHYTETDL KKLRELGATY NQSKGYWVFQ GKPVMPDQFV FELLDSLHRL 850
THLGYQKMKA LLDRGESPYY MLNRDKTLQY VADSCTVCAQ VNASKAKIGA 900
GVRVRGHRPG SHWEIDFTEV KPGLYGYKYL LVFVDTFSGW VEAFPTKRET 950
ARVVSKKLLE EIFPRFGMPQ VLGSDNGPAF TSQVSQSVAD LLGIDWKLHC 1000
AYRPQSSGQV ERMNRTIKET LTKLTLAAGT RDWVLLLPLA LYRARNTPGP 1050
HGLTPYEILY GAPPPLVNFH DPDMSELTNS PSLQAHLQAL QTVQREIWKP 1100
LAEAYRDQLD QPVIPHPFRI GDSVWVRRHQ TKNLEPRWKG PYTVLLTTPT 1150
ALKVDGISAW IHAAHVKAAT TPPIKPSWRV QRSQNPLKIR LTRGAP 1196
Length:1,196
Mass (Da):133,413
Last modified:May 4, 2001 - v2
Checksum:iEDBAE9F550839A01
GO

Sequence cautioni

The sequence AAB03091.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti540 – 5401R → T1 Publication
Sequence conflicti996 – 9961Missing1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01998 Genomic RNA. Translation: AAB03091.1. Different initiation.
PIRiA03958. GNVWK.

Keywords - Coding sequence diversityi

RNA suppression of termination

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J01998 Genomic RNA. Translation: AAB03091.1 . Different initiation.
PIRi A03958. GNVWK.

3D structure databases

ProteinModelPortali P03356.
SMRi P03356. Positions 144-594, 623-788.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.40.70.10. 1 hit.
3.30.420.10. 2 hits.
InterProi IPR001969. Aspartic_peptidase_AS.
IPR001584. Integrase_cat-core.
IPR018061. Pept_A2A_retrovirus_sg.
IPR001995. Peptidase_A2_cat.
IPR021109. Peptidase_aspartic_dom.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
IPR000477. RT_dom.
[Graphical view ]
Pfami PF00075. RNase_H. 1 hit.
PF00665. rve. 1 hit.
PF00077. RVP. 1 hit.
PF00078. RVT_1. 1 hit.
[Graphical view ]
SUPFAMi SSF50630. SSF50630. 1 hit.
SSF53098. SSF53098. 2 hits.
PROSITEi PS50175. ASP_PROT_RETROV. 1 hit.
PS00141. ASP_PROTEASE. 1 hit.
PS50994. INTEGRASE. 1 hit.
PS50879. RNASE_H. 1 hit.
PS50878. RT_POL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of AKV murine leukemia virus."
    Herr W.
    J. Virol. 49:471-478(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 353-1196.

Entry informationi

Entry nameiPOL_MLVAV
AccessioniPrimary (citable) accession number: P03356
Secondary accession number(s): P03357
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: May 4, 2001
Last modified: May 14, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is synthesized as a Gag-Pol polyprotein.

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi