Reviewed, UniProtKB/Swiss-Prot P03356 (POL_MLVAV)

Names and origin

Protein names

Recommended name:
    Pol polyprotein
Cleaved into the following 3 chains:
    1- Recommended name:
            Protease
              EC=3.4.23.-
    2- Recommended name:
            Reverse transcriptase/ribonuclease H
                Short name=RT
              EC=2.7.7.49
              EC=3.1.26.4
    3- Recommended name:
            Integrase
                Short name=IN

Gene names

Name:

pol

Organism

AKV murine leukemia virus (AKR (endogenous) murine leukemia virus)

Taxonomic identifier

11791 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus › Murine leukemia virus

Virus host

Mus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length	1196 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	During replicative cycle of retroviruses, the reverse-transcribed viral DNA is integrated into the host chromosome by the viral integrase enzyme. RNase H activity is associated with the reverse transcriptase.
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins.
Miscellaneous	This protein is synthesized as a Gag-Pol polyprotein.
Sequence similarities	Belongs to the retroviral Pol polyprotein family. Contains 1 integrase catalytic domain. Contains 1 peptidase A2 domain. Contains 1 reverse transcriptase domain. Contains 1 RNase H domain.

Ontologies

Keywords
Biological process	DNA integration DNA recombination
Molecular function	Aspartyl protease Endonuclease Hydrolase Nuclease Nucleotidyltransferase Protease RNA-directed DNA polymerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA integration Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW RNA-dependent DNA replication Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro transposition Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1196	1196	Pol polyprotein		PRO_0000259722
	Chain	1 – 103	103	Protease		PRO_0000026129
	Chain	104 – ?		Reverse transcriptase/ribonuclease H		PRO_0000259723
	Chain	? – 1196		Integrase		PRO_0000259724
Regions
	Domain	22 – 92	71	Peptidase A2
	Domain	202 – 393	192	Reverse transcriptase
	Domain	635 – 781	147	RNase H
	Domain	905 – 1063	159	Integrase catalytic
Sites
	Active site	27	1	By similarity
Experimental info
	Sequence conflict	540	1	R → T Ref.2
	Sequence conflict	996	1	Missing Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P03356-1 [UniParc]. Last modified May 4, 2001. Version 2. Checksum: EDBAE9F550839A01	FASTA	1,196	133,413
	10 20 30 40 50 60 GGQGQEPPPE PRITLTVGGQ PVTFLVDTGA QHSVLTQNPG PLSDRSAWVQ GATGGKRYRW 70 80 90 100 110 120 TTDRKVHLAT GKVTHSFLHV PDCPYPLLGR DLLTKLKAQI HFEGSGAQVV GPKGQPLQVL 130 140 150 160 170 180 TLNLEDEYRL YETSAEPEVS PGSTWLSDFP QAWAETGGMG LAVRQAPLII PLKATSTPVS 190 200 210 220 230 240 IKQYPMSQEA KLGIKPHIQR LLDQGILVPC QSPWNTPLLP VKKPGTNDYR PVQDLREVNK 250 260 270 280 290 300 RVEDIHPTVP NPYNLLSGLP PSHRWYTVLD LKDAFFCLRL HPTSQPLFAF EWRDPGMGIS 310 320 330 340 350 360 GQLTWTRLPQ GFKNSPTLFD EALHRDLADF RIQHPDLILL QYVDDILLAA TSELDCQQGT 370 380 390 400 410 420 RALLLTLGNL GYRASAKKAQ LCQKQVKYLG YLLKEGQRWL TEARKETVMG QPTPKTPRQL 430 440 450 460 470 480 REFLGTAGFC RLWIPGFAEM AAPLYPLTKT GTLFNWGPDQ QKAYQEIKQA LLTAPALGLP 490 500 510 520 530 540 DLTKPFELFV DEKQGYAKGV LTQKLGPWRR PVAYLSKKLD PVAAGWPPCL RMVAAIAVLR 550 560 570 580 590 600 KDAGKLTMGQ PLVILAPHAV EALVKQPPDR WLSNARMTHY QAMLLDTDRV QFGPVVALNP 610 620 630 640 650 660 ATLLPLPEEG APHDCLEILA ETHGTRPDLT DQPIPDADHT WYTDGSSFLQ EGQRKAGAAV 670 680 690 700 710 720 TTETEVIWAR ALPAGTSAQR AELIALTQAL KMAEGKRLNV YTDSRYAFAT AHIHGEIYRR 730 740 750 760 770 780 RGLLTSEGRE IKNKSEILAL LKALFLPKRL SIIHCLGHQK GDSAEARGNR LADQAAREAA 790 800 810 820 830 840 IKTPPDTSTL LIEDSTPYTP AYFHYTETDL KKLRELGATY NQSKGYWVFQ GKPVMPDQFV 850 860 870 880 890 900 FELLDSLHRL THLGYQKMKA LLDRGESPYY MLNRDKTLQY VADSCTVCAQ VNASKAKIGA 910 920 930 940 950 960 GVRVRGHRPG SHWEIDFTEV KPGLYGYKYL LVFVDTFSGW VEAFPTKRET ARVVSKKLLE 970 980 990 1000 1010 1020 EIFPRFGMPQ VLGSDNGPAF TSQVSQSVAD LLGIDWKLHC AYRPQSSGQV ERMNRTIKET 1030 1040 1050 1060 1070 1080 LTKLTLAAGT RDWVLLLPLA LYRARNTPGP HGLTPYEILY GAPPPLVNFH DPDMSELTNS 1090 1100 1110 1120 1130 1140 PSLQAHLQAL QTVQREIWKP LAEAYRDQLD QPVIPHPFRI GDSVWVRRHQ TKNLEPRWKG 1150 1160 1170 1180 1190 PYTVLLTTPT ALKVDGISAW IHAAHVKAAT TPPIKPSWRV QRSQNPLKIR LTRGAP

References

[1]	"Nucleotide sequence of AKV murine leukemia virus." Herr W. J. Virol. 49:471-478(1984) [PubMed: 6319746] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Nucleotide sequence of the 3' half of AKV." Herr W., Corbin V., Gilbert W. Nucleic Acids Res. 10:6931-6944(1982) [PubMed: 6294621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 353-1196.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J01998 Genomic RNA. Translation: AAB03091.1. Different initiation.
PIR	GNVWK. A03958.
3D structure databases
HSSP	HSSP built from PDB template 1I6J based on UniProtKB P03355.
SMR	P03356. Positions 144-594, 623-788.
ModBase	Search...
Protein family/group databases
MEROPS	A02.008.
Family and domain databases
InterPro	IPR000477. DNA_pol_RVTase. IPR001584. Integrase_cat-core. IPR018061. Pept_A2A_retrovirus_sg. IPR001995. Peptidase_A2_cat. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. IPR002156. RNase_H. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
Pfam	PF00075. RnaseH. 1 hit. PF00665. rve. 1 hit. PF00077. RVP. 1 hit. PF00078. RVT_1. 1 hit. [Graphical view]
PROSITE	PS50175. ASP_PROT_RETROV. 1 hit. PS00141. ASP_PROTEASE. 1 hit. PS50994. INTEGRASE. 1 hit. PS50879. RNASE_H. 1 hit. PS50878. RT_POL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POL_MLVAV

Accession

Primary (citable) accession number: P03356
Secondary accession number(s): P03357

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	May 4, 2001
Last modified:	June 16, 2009
This is version 81 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)