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Protein

Gag-Pro polyprotein

Gene

gag-pro

Organism
Human T-cell leukemia virus 2 (HTLV-2)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Gag-Pro polyprotein: The matrix domain targets Gag, Gag-Pro and Gag-Pro-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus.By similarity
Matrix protein p19: Matrix protein.By similarity
Capsid protein p24: Forms the spherical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.By similarity
Nucleocapsid protein p15-pro: Binds strongly to viral nucleic acids and promote their aggregation. Also destabilizes the nucleic acids duplexes via highly structured zinc-binding motifs.By similarity
Protease: The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell (Potential). Cleaves the translation initiation factor eIF4G leading to the inhibition of host cap-dependent translation (By similarity).PROSITE-ProRule annotationBy similarity

Miscellaneous

HTLV-1 lineages are divided in four clades, A (Cosmopolitan), B (Central African group), C (Melanesian group) and D (New Central African group).Curated

Caution

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei478Protease; shared with dimeric partnerPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri361 – 378CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri384 – 401CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease, Viral nucleoprotein
Biological processEukaryotic host gene expression shutoff by virus, Eukaryotic host translation shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiA02.018

Names & Taxonomyi

Protein namesi
Recommended name:
Gag-Pro polyprotein
Alternative name(s):
Pr76Gag-Pro
Cleaved into the following 6 chains:
Matrix protein p19
Short name:
MA
Capsid protein p24
Short name:
CA
Nucleocapsid protein p15-pro
Short name:
NC'
Short name:
NC-pro
Protease (EC:3.4.23.-PROSITE-ProRule annotation)
Short name:
PR
Transframe peptide
Short name:
TFP
Alternative name(s):
p8
Gene namesi
Name:gag-pro
OrganismiHuman T-cell leukemia virus 2 (HTLV-2)
Taxonomic identifieri11909 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeDeltaretrovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009254 Componenti: Genome

Subcellular locationi

Matrix protein p19 :
  • Virion By similarity
Capsid protein p24 :
  • Virion By similarity
Nucleocapsid protein p15-pro :
  • Virion By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostSequence analysis
ChainiPRO_00002598092 – 602Gag-Pro polyproteinAdd BLAST601
ChainiPRO_00002598102 – 136Matrix protein p19Add BLAST135
ChainiPRO_0000259811137 – 350Capsid protein p24Add BLAST214
ChainiPRO_0000259812351 – 446Nucleocapsid protein p15-proAdd BLAST96
ChainiPRO_0000199535447 – 571ProteaseAdd BLAST125
PeptideiPRO_0000259813572 – 579p18
ChainiPRO_0000259814580 – 602Transframe peptideAdd BLAST23

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by hostSequence analysis1

Post-translational modificationi

Gag-Pro polyprotein: Specific enzymatic cleavages by the viral protease yield mature proteins. The polyprotein is cleaved during and after budding, this process is termed maturation. The protease is autoproteolytically processed at its N- and C-termini.By similarity
Matrix protein p19: Phosphorylation of the matrix protein p19 by MAPK1 seems to play a role in budding.By similarity
Gag-Pro polyprotein: Myristoylated. Myristoylation of the matrix (MA) domain mediates the transport and binding of Gag polyproteins to the host plasma membrane and is required for the assembly of viral particles.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei136 – 137Cleavage; by viral proteaseBy similarity2
Sitei350 – 351Cleavage; by viral proteaseBy similarity2
Sitei446 – 447Cleavage; by viral proteaseBy similarity2
Sitei571 – 572Cleavage; by viral proteaseBy similarity2
Sitei579 – 580Cleavage; by viral proteaseBy similarity2

Keywords - PTMi

Lipoprotein, Myristate

Interactioni

Subunit structurei

Gag-Pro polyprotein: Homodimer; the homodimers are part of the immature particles. Gag-Pro polyprotein: Interacts with human TSG101 and NEDD4; these interactions are essential for budding and release of viral particles. Matrix protein p19: Homodimer; further assembles as homohexamers.By similarity

Structurei

Secondary structure

1602
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi21 – 3313
Turni40 – 423
Helixi43 – 5412
Turni60 – 634
Turni65 – 673
Helixi68 – 714
Helixi80 – 889
Turni89 – 913
Beta strandi99 – 1035
Beta strandi117 – 1193

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JVRNMR-A1-136[»]
ProteinModelPortaliP03353
SMRiP03353
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03353

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini473 – 551Peptidase A2PROSITE-ProRule annotationAdd BLAST79

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi94 – 97PTAP/PSAP motifBy similarity4
Motifi124 – 127PPXY motifBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi94 – 150Pro-richAdd BLAST57
Compositional biasi453 – 456Poly-Gln4
Compositional biasi585 – 588Poly-Pro4

Domaini

Capsid protein p24: The capsid protein N-terminus seems to be involved in Gag-Gag interactions.By similarity
Gag-Pro polyprotein: Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. Matrix protein p19 contains two L domains: a PTAP/PSAP motif which interacts with the UEV domain of TSG101, and a PPXY motif which binds to the WW domains of the ubiquitin ligase NEDD4.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri361 – 378CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri384 – 401CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

OrthoDBiVOG09000135

Family and domain databases

Gene3Di1.10.1200.301 hit
1.10.185.101 hit
1.10.375.101 hit
2.40.70.101 hit
InterProiView protein in InterPro
IPR001969 Aspartic_peptidase_AS
IPR003139 D_retro_matrix
IPR036989 D_retro_matrix_sf
IPR000721 Gag_p24
IPR001995 Peptidase_A2_cat
IPR021109 Peptidase_aspartic_dom_sf
IPR018061 Retropepsins
IPR008916 Retrov_capsid_C
IPR008919 Retrov_capsid_N
IPR010999 Retrovr_matrix
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF02228 Gag_p19, 1 hit
PF00607 Gag_p24, 1 hit
PF00077 RVP, 1 hit
PF00098 zf-CCHC, 1 hit
SMARTiView protein in SMART
SM00343 ZnF_C2HC, 2 hits
SUPFAMiSSF47836 SSF47836, 1 hit
SSF47943 SSF47943, 1 hit
SSF50630 SSF50630, 1 hit
SSF57756 SSF57756, 1 hit
PROSITEiView protein in PROSITE
PS50175 ASP_PROT_RETROV, 1 hit
PS00141 ASP_PROTEASE, 1 hit
PS50158 ZF_CCHC, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Note: This strategy of translation probably allows the virus to modulate the quantity of each viral protein.Curated
Isoform Gag-Pro polyprotein (identifier: P03353-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQIHGLSPT PIPKAPRGLS THHWLNFLQA AYRLQPRPSD FDFQQLRRFL
60 70 80 90 100
KLALKTPIWL NPIDYSLLAS LIPKGYPGRV VEIINILVKN QVSPSAPAAP
110 120 130 140 150
VPTPICPTTT PPPPPPPSPE AHVPPPYVEP TTTQCFPILH PPGAPSAHRP
160 170 180 190 200
WQMKDLQAIK QEVSSSALGS PQFMQTLRLA VQQFDPTAKD LQDLLQYLCS
210 220 230 240 250
SLVVSLHHQQ LNTLITEAET RGMTGYNPMA GPLRMQANNP AQQGLRREYQ
260 270 280 290 300
NLWLAAFSTL PGNTRDPSWA AILQGLEEPY CAFVERLNVA LDNGLPEGTP
310 320 330 340 350
KEPILRSLAY SNANKECQKI LQARGHTNSP LGEMLRTCQA WTPKDKTKVL
360 370 380 390 400
VVQPRRPPPT QPCFRCGKVG HWSRDCTQPR PPPGPCPLCQ DPSHWKRDCP
410 420 430 440 450
QLKPPQEEGE PLLLDLPSTS GTTEEKNLLK GGDLISPHPD QDISILPLIP
460 470 480 490 500
LRQQQQPILG VRISVMGQTP QPTQALLDTG ADLTVIPQTL VPGPVKLHDT
510 520 530 540 550
LILGASGQTN TQFKLLQTPL HIFLPFRRSP VILSSCLLDT HNKWTIIGRD
560 570 580 590 600
ALQQCQGLLY LPDDPSPHQL LPIATPNTIG LEHLPPPPQV DQFPLNLSAS

RP
Note: Produced by -1 ribosomal frameshifting at the gag-pro genes boundary.3 Publications
Length:602
Mass (Da):66,435
Last modified:January 23, 2007 - v3
Checksum:i15627B078EFAC16E
GO
Isoform Gag polyprotein (identifier: P03346-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P03346.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by conventional translation.3 Publications
Length:433
Mass (Da):48,028
GO
Isoform Gag-Pro-Pol polyprotein (identifier: P03363-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P03363.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by -1 ribosomal frameshifting at the gag-pol genes boundary.3 Publications
Length:1,461
Mass (Da):162,402
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10060 Genomic DNA No translation available.
PIRiA03954 PNLJH2

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Similar proteinsi

Entry informationi

Entry nameiPRO_HTLV2
AccessioniPrimary (citable) accession number: P03353
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: February 28, 2018
This is version 102 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome