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P03341 (GAG_BAEVM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein

Cleaved into the following 4 chains:

  1. Matrix protein p15
    Short name=MA
  2. RNA-binding phosphoprotein p12
    Alternative name(s):
    pp12
  3. Capsid protein p30
    Short name=CA
  4. Nucleocapsid protein p10
    Short name=NC-gag
Gene names
Name:gag
OrganismBaboon endogenous virus (strain M7) [Complete proteome]
Taxonomic identifier11764 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
Virus hostPapio (baboons) [TaxID: 9554]
Theropithecus gelada (Gelada baboon) [TaxID: 9565]

Protein attributes

Sequence length537 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release By similarity.

Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.

Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex By similarity.

Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization By similarity.

Subunit structure

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers By similarity.

Subcellular location

Gag polyprotein: Virion By similarity. Host cell membrane; Lipid-anchor Potential.

Matrix protein p15: Virion Potential.

Capsid protein p30: Virion Potential.

Nucleocapsid protein p10: Virion Potential.

Domain

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains two L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase and a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101 By similarity.

Post-translational modification

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.

Sequence similarities

Contains 1 CCHC-type zinc finger.

Sequence caution

The sequence J02034 differs from that shown. Reason: Several errors.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed; by host By similarity
Chain2 – 537536Gag polyprotein
PRO_0000390797
Chain2 – 145144Matrix protein p15 Potential
PRO_0000040826
Chain146 – 22681RNA-binding phosphoprotein p12 Potential
PRO_0000040827
Chain227 – 477251Capsid protein p30 Potential
PRO_0000040828
Chain478 – 53760Nucleocapsid protein p10 Potential
PRO_0000040829

Regions

Zinc finger501 – 51818CCHC-type
Motif154 – 1574PPXY motif
Motif173 – 1764PTAP/PSAP motif

Sites

Site226 – 2272Cleavage; by viral protease By similarity
Site477 – 4782Cleavage; by viral protease By similarity

Amino acid modifications

Lipidation21N-myristoyl glycine; by host By similarity

Sequences

Sequence LengthMass (Da)Tools
P03341 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 5DEEE4437CFCFB79

FASTA53760,624
        10         20         30         40         50         60 
MGQTLTTPLS LTLTHFSDVR ARAHNLSVGV RKGRWQTFCS SEWPTLHVGW PRDGTFDLSV 

        70         80         90        100        110        120 
ILQVKTKVMD PGPHGHPDQV AYIITWEDLV RNPPPWVKPF LHTPSTSKST LLALEVPKNR 

       130        140        150        160        170        180 
TLDPPKPVLP DESQQDLLFQ DPLPHPPHNP LLEPPPYNSP SPPVLSPVSP TTPSAPTPSS 

       190        200        210        220        230        240 
LVSSSTPPSS PAPPELTPRT PPQTPRLRLR RAEGQDGPST WQSSLFPLRT VNRTIQYWPF 

       250        260        270        280        290        300 
SASDLYNWKT HNPSFSQDPQ ALTSLIESIL LTHQPTWDDC QQLLQVLLTT EERQRVLLEA 

       310        320        330        340        350        360 
RKNVPGPGGL PTQLPNEIDE GFPLTRPDWD YETAPGRESL RIYRQALLAG LKGAGKRPTN 

       370        380        390        400        410        420 
LAKVRTITQG KDESPAAFME RLLEGFRMYT PFDPEAPEHK ATVAMSFIDQ AALDIKGKLQ 

       430        440        450        460        470        480 
RLDGIQTHGL QELVREAEKV YNKRETPEER EARLIKEQEE REDRRDRKRD KHLTKILAAV 

       490        500        510        520        530 
VTEKRAGKSG ETRRRPKVDK DQCAYCKERG HWIKDCPKRP RDQKKPAPVL TLGEDSE 

« Hide

References

[1]"The entire nucleotide sequence of baboon endogenous virus DNA: a chimeric genome structure of murine type C and simian type D retroviruses."
Kato S., Matsuo K., Nishimura N., Takahashi N., Takano T.
Jpn. J. Genet. 62:127-137(1987)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Provirus of M7 baboon endogenous virus: nucleotide sequence of the gag-pol region."
Tamura T.
J. Virol. 47:137-145(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10032 Genomic DNA. Translation: BAA00923.1.
X05470 Genomic DNA. Translation: CAA29027.1.
J02034 Genomic DNA. No translation available.
PIRFOMVVB. A03939.
FOMVM7. JT0260.

3D structure databases

ProteinModelPortalP03341.
SMRP03341. Positions 2-98, 227-356, 361-392, 493-528.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProIPR000840. G_retro_matrix_N.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view]
PfamPF01140. Gag_MA. 1 hit.
PF02093. Gag_p30. 1 hit.
[Graphical view]
SMARTSM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEPS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGAG_BAEVM
AccessionPrimary (citable) accession number: P03341
Secondary accession number(s): P10268
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 103 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families