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P03341

- GAG_BAEVM

UniProt

P03341 - GAG_BAEVM

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Protein
Gag polyprotein
Gene
gag
Organism
Baboon endogenous virus (strain M7)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release By similarity.
Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.
Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex By similarity.
Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei226 – 2272Cleavage; by viral protease By similarity
Sitei477 – 4782Cleavage; by viral protease By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri501 – 51818CCHC-type
Add
BLAST

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural constituent of virion Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. viral budding via host ESCRT complex Source: UniProtKB-KW
  2. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

Keywords - Ligandi

Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 4 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Capsid protein p30
Short name:
CA
Nucleocapsid protein p10
Short name:
NC-gag
Gene namesi
Name:gag
OrganismiBaboon endogenous virus (strain M7)
Taxonomic identifieri11764 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
Virus hostiPapio (baboons) [TaxID: 9554]
Theropithecus gelada (Gelada baboon) [TaxID: 9565]
ProteomesiUP000007443: Genome

Subcellular locationi

Chain Gag polyprotein : Virion By similarity. Host cell membrane; Lipid-anchor Reviewed prediction
Chain Matrix protein p15 : Virion Reviewed prediction
Chain Capsid protein p30 : Virion Reviewed prediction

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. membrane Source: UniProtKB-KW
  3. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by host By similarity
Chaini2 – 537536Gag polyprotein
PRO_0000390797Add
BLAST
Chaini2 – 145144Matrix protein p15 Reviewed prediction
PRO_0000040826Add
BLAST
Chaini146 – 22681RNA-binding phosphoprotein p12 Reviewed prediction
PRO_0000040827Add
BLAST
Chaini227 – 477251Capsid protein p30 Reviewed prediction
PRO_0000040828Add
BLAST
Chaini478 – 53760Nucleocapsid protein p10 Reviewed prediction
PRO_0000040829Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine; by host By similarity

Post-translational modificationi

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.

Keywords - PTMi

Lipoprotein, Myristate

Interactioni

Subunit structurei

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers By similarity.

Structurei

3D structure databases

ProteinModelPortaliP03341.
SMRiP03341. Positions 2-98, 227-356, 361-392, 493-528.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi154 – 1574PPXY motif
Motifi173 – 1764PTAP/PSAP motif

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains two L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase and a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101 By similarity.

Sequence similaritiesi

Keywords - Domaini

Zinc-finger

Family and domain databases

Gene3Di1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR000840. G_retro_matrix_N.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF01140. Gag_MA. 1 hit.
PF02093. Gag_p30. 1 hit.
[Graphical view]
SMARTiSM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03341-1 [UniParc]FASTAAdd to Basket

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MGQTLTTPLS LTLTHFSDVR ARAHNLSVGV RKGRWQTFCS SEWPTLHVGW    50
PRDGTFDLSV ILQVKTKVMD PGPHGHPDQV AYIITWEDLV RNPPPWVKPF 100
LHTPSTSKST LLALEVPKNR TLDPPKPVLP DESQQDLLFQ DPLPHPPHNP 150
LLEPPPYNSP SPPVLSPVSP TTPSAPTPSS LVSSSTPPSS PAPPELTPRT 200
PPQTPRLRLR RAEGQDGPST WQSSLFPLRT VNRTIQYWPF SASDLYNWKT 250
HNPSFSQDPQ ALTSLIESIL LTHQPTWDDC QQLLQVLLTT EERQRVLLEA 300
RKNVPGPGGL PTQLPNEIDE GFPLTRPDWD YETAPGRESL RIYRQALLAG 350
LKGAGKRPTN LAKVRTITQG KDESPAAFME RLLEGFRMYT PFDPEAPEHK 400
ATVAMSFIDQ AALDIKGKLQ RLDGIQTHGL QELVREAEKV YNKRETPEER 450
EARLIKEQEE REDRRDRKRD KHLTKILAAV VTEKRAGKSG ETRRRPKVDK 500
DQCAYCKERG HWIKDCPKRP RDQKKPAPVL TLGEDSE 537
Length:537
Mass (Da):60,624
Last modified:January 23, 2007 - v4
Checksum:i5DEEE4437CFCFB79
GO

Sequence cautioni

The sequence J02034 differs from that shown. Reason: Several errors.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10032 Genomic DNA. Translation: BAA00923.1.
X05470 Genomic DNA. Translation: CAA29027.1.
J02034 Genomic DNA. No translation available.
PIRiA03939. FOMVVB.
JT0260. FOMVM7.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10032 Genomic DNA. Translation: BAA00923.1 .
X05470 Genomic DNA. Translation: CAA29027.1 .
J02034 Genomic DNA. No translation available.
PIRi A03939. FOMVVB.
JT0260. FOMVM7.

3D structure databases

ProteinModelPortali P03341.
SMRi P03341. Positions 2-98, 227-356, 361-392, 493-528.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProi IPR000840. G_retro_matrix_N.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view ]
Pfami PF01140. Gag_MA. 1 hit.
PF02093. Gag_p30. 1 hit.
[Graphical view ]
SMARTi SM00343. ZnF_C2HC. 1 hit.
[Graphical view ]
SUPFAMi SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEi PS50158. ZF_CCHC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The entire nucleotide sequence of baboon endogenous virus DNA: a chimeric genome structure of murine type C and simian type D retroviruses."
    Kato S., Matsuo K., Nishimura N., Takahashi N., Takano T.
    Jpn. J. Genet. 62:127-137(1987)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Provirus of M7 baboon endogenous virus: nucleotide sequence of the gag-pol region."
    Tamura T.
    J. Virol. 47:137-145(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGAG_BAEVM
AccessioniPrimary (citable) accession number: P03341
Secondary accession number(s): P10268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 103 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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