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P03341

- GAG_BAEVM

UniProt

P03341 - GAG_BAEVM

Protein

Gag polyprotein

Gene

gag

Organism
Baboon endogenous virus (strain M7)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release By similarity.By similarity
    Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.By similarity
    Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity
    Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei226 – 2272Cleavage; by viral proteaseBy similarity
    Sitei477 – 4782Cleavage; by viral proteaseBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri501 – 51818CCHC-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. structural constituent of virion Source: UniProtKB-KW
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. viral budding via host ESCRT complex Source: UniProtKB-KW
    2. viral release from host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

    Keywords - Ligandi

    Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gag polyprotein
    Alternative name(s):
    Core polyprotein
    Cleaved into the following 4 chains:
    Matrix protein p15
    Short name:
    MA
    Alternative name(s):
    pp12
    Capsid protein p30
    Short name:
    CA
    Nucleocapsid protein p10
    Short name:
    NC-gag
    Gene namesi
    Name:gag
    OrganismiBaboon endogenous virus (strain M7)
    Taxonomic identifieri11764 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
    Virus hostiPapio (baboons) [TaxID: 9554]
    Theropithecus gelada (Gelada baboon) [TaxID: 9565]
    ProteomesiUP000007443: Genome

    Subcellular locationi

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-KW
    3. viral nucleocapsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed; by hostBy similarity
    Chaini2 – 537536Gag polyproteinPRO_0000390797Add
    BLAST
    Chaini2 – 145144Matrix protein p15Sequence AnalysisPRO_0000040826Add
    BLAST
    Chaini146 – 22681RNA-binding phosphoprotein p12Sequence AnalysisPRO_0000040827Add
    BLAST
    Chaini227 – 477251Capsid protein p30Sequence AnalysisPRO_0000040828Add
    BLAST
    Chaini478 – 53760Nucleocapsid protein p10Sequence AnalysisPRO_0000040829Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycine; by hostBy similarity

    Post-translational modificationi

    Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.By similarity

    Keywords - PTMi

    Lipoprotein, Myristate

    Interactioni

    Subunit structurei

    Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP03341.
    SMRiP03341. Positions 2-98, 227-356, 361-392, 493-528.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi154 – 1574PPXY motif
    Motifi173 – 1764PTAP/PSAP motif

    Domaini

    Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains two L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase and a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101 By similarity.By similarity

    Sequence similaritiesi

    Contains 1 CCHC-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri501 – 51818CCHC-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    Gene3Di1.10.150.180. 1 hit.
    1.10.375.10. 1 hit.
    4.10.60.10. 1 hit.
    InterProiIPR000840. G_retro_matrix_N.
    IPR003036. Gag_P30.
    IPR008919. Retrov_capsid_N.
    IPR010999. Retrovr_matrix_N.
    IPR001878. Znf_CCHC.
    [Graphical view]
    PfamiPF01140. Gag_MA. 1 hit.
    PF02093. Gag_p30. 1 hit.
    [Graphical view]
    SMARTiSM00343. ZnF_C2HC. 1 hit.
    [Graphical view]
    SUPFAMiSSF47836. SSF47836. 1 hit.
    SSF47943. SSF47943. 1 hit.
    SSF57756. SSF57756. 1 hit.
    PROSITEiPS50158. ZF_CCHC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P03341-1 [UniParc]FASTAAdd to Basket

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    MGQTLTTPLS LTLTHFSDVR ARAHNLSVGV RKGRWQTFCS SEWPTLHVGW    50
    PRDGTFDLSV ILQVKTKVMD PGPHGHPDQV AYIITWEDLV RNPPPWVKPF 100
    LHTPSTSKST LLALEVPKNR TLDPPKPVLP DESQQDLLFQ DPLPHPPHNP 150
    LLEPPPYNSP SPPVLSPVSP TTPSAPTPSS LVSSSTPPSS PAPPELTPRT 200
    PPQTPRLRLR RAEGQDGPST WQSSLFPLRT VNRTIQYWPF SASDLYNWKT 250
    HNPSFSQDPQ ALTSLIESIL LTHQPTWDDC QQLLQVLLTT EERQRVLLEA 300
    RKNVPGPGGL PTQLPNEIDE GFPLTRPDWD YETAPGRESL RIYRQALLAG 350
    LKGAGKRPTN LAKVRTITQG KDESPAAFME RLLEGFRMYT PFDPEAPEHK 400
    ATVAMSFIDQ AALDIKGKLQ RLDGIQTHGL QELVREAEKV YNKRETPEER 450
    EARLIKEQEE REDRRDRKRD KHLTKILAAV VTEKRAGKSG ETRRRPKVDK 500
    DQCAYCKERG HWIKDCPKRP RDQKKPAPVL TLGEDSE 537
    Length:537
    Mass (Da):60,624
    Last modified:January 23, 2007 - v4
    Checksum:i5DEEE4437CFCFB79
    GO

    Sequence cautioni

    The sequence J02034 differs from that shown. Reason: Several errors.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10032 Genomic DNA. Translation: BAA00923.1.
    X05470 Genomic DNA. Translation: CAA29027.1.
    J02034 Genomic DNA. No translation available.
    PIRiA03939. FOMVVB.
    JT0260. FOMVM7.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10032 Genomic DNA. Translation: BAA00923.1 .
    X05470 Genomic DNA. Translation: CAA29027.1 .
    J02034 Genomic DNA. No translation available.
    PIRi A03939. FOMVVB.
    JT0260. FOMVM7.

    3D structure databases

    ProteinModelPortali P03341.
    SMRi P03341. Positions 2-98, 227-356, 361-392, 493-528.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.150.180. 1 hit.
    1.10.375.10. 1 hit.
    4.10.60.10. 1 hit.
    InterProi IPR000840. G_retro_matrix_N.
    IPR003036. Gag_P30.
    IPR008919. Retrov_capsid_N.
    IPR010999. Retrovr_matrix_N.
    IPR001878. Znf_CCHC.
    [Graphical view ]
    Pfami PF01140. Gag_MA. 1 hit.
    PF02093. Gag_p30. 1 hit.
    [Graphical view ]
    SMARTi SM00343. ZnF_C2HC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47836. SSF47836. 1 hit.
    SSF47943. SSF47943. 1 hit.
    SSF57756. SSF57756. 1 hit.
    PROSITEi PS50158. ZF_CCHC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The entire nucleotide sequence of baboon endogenous virus DNA: a chimeric genome structure of murine type C and simian type D retroviruses."
      Kato S., Matsuo K., Nishimura N., Takahashi N., Takano T.
      Jpn. J. Genet. 62:127-137(1987)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Provirus of M7 baboon endogenous virus: nucleotide sequence of the gag-pol region."
      Tamura T.
      J. Virol. 47:137-145(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiGAG_BAEVM
    AccessioniPrimary (citable) accession number: P03341
    Secondary accession number(s): P10268
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 104 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3