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P03340 (GAG_FSVMD) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein

Cleaved into the following 4 chains:

  1. Matrix protein p15
    Short name=MA
  2. RNA-binding phosphoprotein p12
    Alternative name(s):
    pp12
  3. Capsid protein p30
    Short name=CA
  4. Nucleocapsid protein p10
    Short name=NC-gag
Gene names
Name:gag
OrganismFeline sarcoma virus (strain McDonough)
Taxonomic identifier11778 [NCBI]
Taxonomic lineageViruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus ›
Virus hostFelidae (cat family) [TaxID: 9681]

Protein attributes

Sequence length536 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release By similarity.

Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.

Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex By similarity.

Subunit structure

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers By similarity.

Subcellular location

Gag polyprotein: Virion By similarity. Host cell membrane; Lipid-anchor Potential.

Matrix protein p15: Virion Potential.

Capsid protein p30: Virion Potential.

Domain

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 also contains one L domain: a LYPX(n)L which potentially interacts with PDCD6IP By similarity.

Post-translational modification

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.

Miscellaneous

This protein is synthesized as a Gag-Fms polyprotein.

Caution

This gag polyprotein encodes a truncated nucleoprotein.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 536536Gag polyprotein
PRO_0000390802
Propeptide1 – 7777Leader peptide Potential
PRO_0000040851
Chain78 – 204127Matrix protein p15 Potential
PRO_0000040852
Chain205 – 27470RNA-binding phosphoprotein p12 Potential
PRO_0000040853
Chain275 – 522248Capsid protein p30 Potential
PRO_0000040854
Chain523 – 53614Nucleocapsid protein p10 Potential
PRO_0000040855

Regions

Motif195 – 1984PTAP/PSAP motif
Motif203 – 2075LYPX(n)L motif
Motif234 – 2374PPXY motif

Sites

Site204 – 2052Cleavage; by viral protease By similarity
Site274 – 2752Cleavage; by viral protease By similarity
Site522 – 5232Cleavage; by viral protease By similarity

Amino acid modifications

Lipidation791N-myristoyl glycine; by host By similarity

Sequences

Sequence LengthMass (Da)Tools
P03340 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: FBBC80D612AC8702

FASTA53660,235
        10         20         30         40         50         60 
MSGASSGTAT GARLFGISSV LGEYRVLIGD EGAGPSRSPS EVSFSVWYRS RAARLVILCL 

        70         80         90        100        110        120 
VASFLVPCLT FLIAETVMGQ TVTTPPSLTL DHWSEVRTRA HNQGIEVRKK KWITLCEAEW 

       130        140        150        160        170        180 
VMMNVGWPRE GTPPLDNTSQ VEKRIFAPGP HGHPDQVPYI TTWRSLATDP PSWVRPFLPP 

       190        200        210        220        230        240 
PKPPTPLPQP LSPQPSAPPT SSLYPVLPKT NPPKPPVLPP DPSSPLIDLL TEEPPPYPGG 

       250        260        270        280        290        300 
HGPPPSGLRT PAASPIASRL RERRENPAEE SQALPLREGP NNRPQYWPFS ASDLYNWKLH 

       310        320        330        340        350        360 
NPPFSQDPVA LTNLIESILV THQPTWDDCQ QLLQALLTAE ERQRVLLEAR KQVPGEDGRP 

       370        380        390        400        410        420 
TQLPNVIDEA FPLTRPNWDF ATPAGREHLR LYRQLLLAGL RGAARRPTNL AQVKQVVQGK 

       430        440        450        460        470        480 
EETPASFLER LKEAYRMYTP YDPEDPGQAA SVILSFIYQS SPDIRNKLQR LEGLQGFTLS 

       490        500        510        520        530 
DLLKEAEKIY NKRETPEERE ERLWQRQEER DKKRHKEMTK VLATVVTQNR NKDREE

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References

[1]"Nucleotide sequence of the feline retroviral oncogene v-fms shows unexpected homology with oncogenes encoding tyrosine-specific protein kinases."
Hampe A., Gobet M., Sherr C.J., Galibert F.
Proc. Natl. Acad. Sci. U.S.A. 81:85-89(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		K01643 Genomic RNA. Translation: AAA43045.1. Different termination.
PIRFOMVMD. A03938.

3D structure databases

ProteinModelPortalP03340.
SMRP03340. Positions 79-175, 275-405, 411-441.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
InterProIPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR003036. Gag_p30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
[Graphical view]
PfamPF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
[Graphical view]
SUPFAMSSF47943. Retrov_capsid_N. 1 hit.
SSF47836. Retrovir_matrix. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGAG_FSVMD
AccessionPrimary (citable) accession number: P03340
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program
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