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P03340

- GAG_FSVMD

UniProt

P03340 - GAG_FSVMD

Protein

Gag polyprotein

Gene

gag

Organism
Feline sarcoma virus (strain McDonough)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release By similarity.By similarity
    Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.By similarity
    Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei204 – 2052Cleavage; by viral proteaseBy similarity
    Sitei274 – 2752Cleavage; by viral proteaseBy similarity
    Sitei522 – 5232Cleavage; by viral proteaseBy similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. structural constituent of virion Source: UniProtKB-KW

    GO - Biological processi

    1. viral budding via host ESCRT complex Source: UniProtKB-KW
    2. viral release from host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

    Keywords - Ligandi

    RNA-binding, Viral nucleoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gag polyprotein
    Alternative name(s):
    Core polyprotein
    Cleaved into the following 4 chains:
    Matrix protein p15
    Short name:
    MA
    Alternative name(s):
    pp12
    Capsid protein p30
    Short name:
    CA
    Nucleocapsid protein p10
    Short name:
    NC-gag
    Gene namesi
    Name:gag
    OrganismiFeline sarcoma virus (strain McDonough)
    Taxonomic identifieri11778 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirus
    Virus hostiFelidae (cat family) [TaxID: 9681]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-KW
    3. viral nucleocapsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 536536Gag polyproteinPRO_0000390802Add
    BLAST
    Propeptidei1 – 7777Leader peptideSequence AnalysisPRO_0000040851Add
    BLAST
    Chaini78 – 204127Matrix protein p15Sequence AnalysisPRO_0000040852Add
    BLAST
    Chaini205 – 27470RNA-binding phosphoprotein p12Sequence AnalysisPRO_0000040853Add
    BLAST
    Chaini275 – 522248Capsid protein p30Sequence AnalysisPRO_0000040854Add
    BLAST
    Chaini523 – 53614Nucleocapsid protein p10Sequence AnalysisPRO_0000040855Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi79 – 791N-myristoyl glycine; by hostBy similarity

    Post-translational modificationi

    Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.By similarity

    Keywords - PTMi

    Lipoprotein, Myristate

    Interactioni

    Subunit structurei

    Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP03340.
    SMRiP03340. Positions 79-175, 275-405, 411-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi195 – 1984PTAP/PSAP motif
    Motifi203 – 2075LYPX(n)L motif
    Motifi234 – 2374PPXY motif

    Domaini

    Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 also contains one L domain: a LYPX(n)L which potentially interacts with PDCD6IP By similarity.By similarity

    Family and domain databases

    Gene3Di1.10.150.180. 1 hit.
    1.10.375.10. 1 hit.
    InterProiIPR000840. G_retro_matrix_N.
    IPR002079. Gag_p12.
    IPR003036. Gag_P30.
    IPR008919. Retrov_capsid_N.
    IPR010999. Retrovr_matrix_N.
    [Graphical view]
    PfamiPF01140. Gag_MA. 1 hit.
    PF01141. Gag_p12. 1 hit.
    PF02093. Gag_p30. 1 hit.
    [Graphical view]
    SUPFAMiSSF47836. SSF47836. 1 hit.
    SSF47943. SSF47943. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P03340-1 [UniParc]FASTAAdd to Basket

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    MSGASSGTAT GARLFGISSV LGEYRVLIGD EGAGPSRSPS EVSFSVWYRS    50
    RAARLVILCL VASFLVPCLT FLIAETVMGQ TVTTPPSLTL DHWSEVRTRA 100
    HNQGIEVRKK KWITLCEAEW VMMNVGWPRE GTPPLDNTSQ VEKRIFAPGP 150
    HGHPDQVPYI TTWRSLATDP PSWVRPFLPP PKPPTPLPQP LSPQPSAPPT 200
    SSLYPVLPKT NPPKPPVLPP DPSSPLIDLL TEEPPPYPGG HGPPPSGLRT 250
    PAASPIASRL RERRENPAEE SQALPLREGP NNRPQYWPFS ASDLYNWKLH 300
    NPPFSQDPVA LTNLIESILV THQPTWDDCQ QLLQALLTAE ERQRVLLEAR 350
    KQVPGEDGRP TQLPNVIDEA FPLTRPNWDF ATPAGREHLR LYRQLLLAGL 400
    RGAARRPTNL AQVKQVVQGK EETPASFLER LKEAYRMYTP YDPEDPGQAA 450
    SVILSFIYQS SPDIRNKLQR LEGLQGFTLS DLLKEAEKIY NKRETPEERE 500
    ERLWQRQEER DKKRHKEMTK VLATVVTQNR NKDREE 536
    Length:536
    Mass (Da):60,235
    Last modified:July 21, 1986 - v1
    Checksum:iFBBC80D612AC8702
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K01643 Genomic RNA. Translation: AAA43045.1. Different termination.
    PIRiA03938. FOMVMD.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K01643 Genomic RNA. Translation: AAA43045.1 . Different termination.
    PIRi A03938. FOMVMD.

    3D structure databases

    ProteinModelPortali P03340.
    SMRi P03340. Positions 79-175, 275-405, 411-441.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.150.180. 1 hit.
    1.10.375.10. 1 hit.
    InterProi IPR000840. G_retro_matrix_N.
    IPR002079. Gag_p12.
    IPR003036. Gag_P30.
    IPR008919. Retrov_capsid_N.
    IPR010999. Retrovr_matrix_N.
    [Graphical view ]
    Pfami PF01140. Gag_MA. 1 hit.
    PF01141. Gag_p12. 1 hit.
    PF02093. Gag_p30. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47836. SSF47836. 1 hit.
    SSF47943. SSF47943. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the feline retroviral oncogene v-fms shows unexpected homology with oncogenes encoding tyrosine-specific protein kinases."
      Hampe A., Gobet M., Sherr C.J., Galibert F.
      Proc. Natl. Acad. Sci. U.S.A. 81:85-89(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiGAG_FSVMD
    AccessioniPrimary (citable) accession number: P03340
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This protein is synthesized as a Gag-Fms polyprotein.

    Caution

    This gag polyprotein encodes a truncated nucleoprotein.Curated

    External Data

    Dasty 3