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Protein

Gag polyprotein

Gene

gag

Organism
Feline sarcoma virus (strain Snyder-Theilen)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release (By similarity).By similarity
Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity).By similarity
Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity

Miscellaneous

This protein is synthesized as a Gag-Fes polyprotein.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processHost-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 3 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Capsid protein p30
Short name:
CA
Gene namesi
Name:gag
OrganismiFeline sarcoma virus (strain Snyder-Theilen)
Taxonomic identifieri11780 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirus
Virus hostiFelidae (cat family) [TaxID: 9681]

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostBy similarity
ChainiPRO_00003908032 – 297Gag polyproteinAdd BLAST296
ChainiPRO_00000408572 – 127Matrix protein p15Sequence analysisAdd BLAST126
ChainiPRO_0000040858128 – 197RNA-binding phosphoprotein p12Sequence analysisAdd BLAST70
ChainiPRO_0000040859198 – 297Capsid protein p30Sequence analysisAdd BLAST100

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by hostBy similarity1

Post-translational modificationi

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei127 – 128Cleavage; by viral proteaseBy similarity2
Sitei197 – 198Cleavage; by viral proteaseBy similarity2

Keywords - PTMi

Lipoprotein, Myristate

Interactioni

Subunit structurei

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP03338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi118 – 121PTAP/PSAP motif4
Motifi126 – 130LYPX(n)L motif5
Motifi157 – 160PPXY motif4

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 also contains one L domain: a LYPX(n)L which potentially interacts with PDCD6IP (By similarity).By similarity

Family and domain databases

Gene3Di1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
InterProiView protein in InterPro
IPR000840. G_retro_matrix.
IPR036946. G_retro_matrix_sf.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix.
PfamiView protein in Pfam
PF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
SUPFAMiSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Gag polyprotein (identifier: P03338-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQTVTTPLS LTLDHWSEVR ARAHNQGVEV RKKKWITLCK AEWVMMNVGW
60 70 80 90 100
PREGTFSLDN ISQVKKKIFA PGPHGHPDQV PYITTWRSLA TDPPSWVRPF
110 120 130 140 150
LPPPKPPTPL PQPLSPQPSA PLTSSLYPVV PKPDPPKPPV LPPDPSSPLI
160 170 180 190 200
DLLTEEPPPY PGGHGPPPSG PRTPAASPIV SRLRERRENP AEESQALPLR
210 220 230 240 250
EGPNNRPQYW PFSASDLYNW KSHNPPFSQD PVALTNLIES ILVTHQPTWD
260 270 280 290
DCQQLLQALL TGEERQRVLL EARKQVPGED GRPTQLPNVI DETFPLT
Length:297
Mass (Da):33,115
Last modified:August 30, 2017 - v2
Checksum:iA738F562DAECEC57
GO
Isoform Glyco-Gag protein (identifier: P0DOG9-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P0DOG9.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:371
Mass (Da):40,830
GO

Sequence cautioni

P03338: The sequence AAA43046 differs from that shown. Reason: Erroneous gene model prediction.By similarity

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02088 Genomic RNA. Translation: AAA43046.2. Sequence problems.
PIRiA03935. FOMVCS.

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiGAG_FSVST
AccessioniPrimary (citable) accession number: P03338
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 30, 2017
Last modified: October 25, 2017
This is version 90 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program