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P03338

- GAG_FSVST

UniProt

P03338 - GAG_FSVST

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Protein

Gag polyprotein

Gene

gag

Organism
Feline sarcoma virus (strain Snyder-Theilen)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release (By similarity).By similarity
Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity).By similarity
Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei201 – 2022Cleavage; by viral proteaseBy similarity
Sitei271 – 2722Cleavage; by viral proteaseBy similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural constituent of virion Source: UniProtKB-KW

GO - Biological processi

  1. viral budding via host ESCRT complex Source: UniProtKB-KW
  2. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 3 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Capsid protein p30
Short name:
CA
Gene namesi
Name:gag
OrganismiFeline sarcoma virus (strain Snyder-Theilen)
Taxonomic identifieri11780 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirus
Virus hostiFelidae (cat family) [TaxID: 9681]

Subcellular locationi

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
  3. viral capsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 371371Gag polyproteinPRO_0000390803Add
BLAST
Propeptidei1 – 7474Leader peptideSequence AnalysisPRO_0000040856Add
BLAST
Chaini75 – 201127Matrix protein p15Sequence AnalysisPRO_0000040857Add
BLAST
Chaini202 – 27170RNA-binding phosphoprotein p12Sequence AnalysisPRO_0000040858Add
BLAST
Chaini272 – 371100Capsid protein p30Sequence AnalysisPRO_0000040859Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi76 – 761N-myristoyl glycine; by hostBy similarity

Post-translational modificationi

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Myristate

Interactioni

Subunit structurei

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP03338.
SMRiP03338. Positions 76-172, 272-370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi192 – 1954PTAP/PSAP motif
Motifi200 – 2045LYPX(n)L motif
Motifi231 – 2344PPXY motif

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 also contains one L domain: a LYPX(n)L which potentially interacts with PDCD6IP (By similarity).By similarity

Family and domain databases

Gene3Di1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
InterProiIPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
[Graphical view]
PfamiPF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03338-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MSGASSGTAI GAHLFGVSPE YRVLIGDEGA GPSKSLSEVS FSVWYRSRAA
60 70 80 90 100
RLVILCLVAS FLVPCLTFLI AEAVMGQTVT TPLSLTLDHW SEVRARAHNQ
110 120 130 140 150
GVEVRKKKWI TLCKAEWVMM NVGWPREGTF SLDNISQVKK KIFAPGPHGH
160 170 180 190 200
PDQVPYITTW RSLATDPPSW VRPFLPPPKP PTPLPQPLSP QPSAPLTSSL
210 220 230 240 250
YPVVPKPDPP KPPVLPPDPS SPLIDLLTEE PPPYPGGHGP PPSGPRTPAA
260 270 280 290 300
SPIVSRLRER RENPAEESQA LPLREGPNNR PQYWPFSASD LYNWKSHNPP
310 320 330 340 350
FSQDPVALTN LIESILVTHQ PTWDDCQQLL QALLTGEERQ RVLLEARKQV
360 370
PGEDGRPTQL PNVIDETFPL T
Length:371
Mass (Da):40,830
Last modified:July 21, 1986 - v1
Checksum:i5842DCC63483F420
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02088 Genomic RNA. Translation: AAA43046.2. Different termination.
PIRiA03935. FOMVCS.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02088 Genomic RNA. Translation: AAA43046.2 . Different termination.
PIRi A03935. FOMVCS.

3D structure databases

ProteinModelPortali P03338.
SMRi P03338. Positions 76-172, 272-370.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
InterProi IPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
[Graphical view ]
Pfami PF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
[Graphical view ]
SUPFAMi SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequences of feline sarcoma virus long terminal repeats and 5' leaders show extensive homology to those of other mammalian retroviruses."
    Hampe A., Gobet M., Even J., Sherr C.J., Galibert F.
    J. Virol. 45:466-472(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-75.
  2. "Nucleotide sequences of feline retroviral oncogenes (v-fes) provide evidence for a family of tyrosine-specific protein kinase genes."
    Hampe A., Laprevotte I., Galibert F., Fedele L.A., Sherr C.J.
    Cell 30:775-785(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 75-371.

Entry informationi

Entry nameiGAG_FSVST
AccessioniPrimary (citable) accession number: P03338
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 29, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein is synthesized as a Gag-Fes polyprotein.

External Data

Dasty 3