P03338 (GAG_FSVST) Reviewed, UniProtKB/Swiss-Prot
Last modified October 16, 2013. Version 80. History...
Names and origin
|Protein names||Recommended name:|
|Organism||Feline sarcoma virus (strain Snyder-Theilen)|
|Taxonomic identifier||11780 [NCBI]|
|Taxonomic lineage||Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus ›|
|Virus host||Felidae (cat family) [TaxID: 9681]|
|Sequence length||371 AA.|
|Sequence processing||The displayed sequence is further processed into a mature form.|
|Protein existence||Inferred from homology|
General annotation (Comments)
Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release By similarity.
Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.
Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex By similarity.
Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers By similarity.
Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 also contains one L domain: a LYPX(n)L which potentially interacts with PDCD6IP By similarity.
Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.
This protein is synthesized as a Gag-Fes polyprotein.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 371||371||Gag polyprotein||PRO_0000390803|
|Propeptide||1 – 74||74||Leader peptide Potential||PRO_0000040856|
|Chain||75 – 201||127||Matrix protein p15 Potential||PRO_0000040857|
|Chain||202 – 271||70||RNA-binding phosphoprotein p12 Potential||PRO_0000040858|
|Chain||272 – 371||100||Capsid protein p30 Potential||PRO_0000040859|
|Motif||192 – 195||4||PTAP/PSAP motif|
|Motif||200 – 204||5||LYPX(n)L motif|
|Motif||231 – 234||4||PPXY motif|
|Site||201 – 202||2||Cleavage; by viral protease By similarity|
|Site||271 – 272||2||Cleavage; by viral protease By similarity|
Amino acid modifications
|Lipidation||76||1||N-myristoyl glycine; by host By similarity|
|||"Nucleotide sequences of feline sarcoma virus long terminal repeats and 5' leaders show extensive homology to those of other mammalian retroviruses."|
Hampe A., Gobet M., Even J., Sherr C.J., Galibert F.
J. Virol. 45:466-472(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-75.
|||"Nucleotide sequences of feline retroviral oncogenes (v-fes) provide evidence for a family of tyrosine-specific protein kinase genes."|
Hampe A., Laprevotte I., Galibert F., Fedele L.A., Sherr C.J.
Cell 30:775-785(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 75-371.
|J02088 Genomic RNA. Translation: AAA43046.2. Different termination.|
|PIR||FOMVCS. A03935. |
3D structure databases
|SMR||P03338. Positions 76-172, 272-370. |
Protocols and materials databases
Family and domain databases
|Gene3D||126.96.36.199. 1 hit. |
1.10.375.10. 1 hit.
|InterPro||IPR000840. G_retro_matrix_N. |
|Pfam||PF01140. Gag_MA. 1 hit. |
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
|SUPFAM||SSF47836. SSF47836. 1 hit. |
SSF47943. SSF47943. 1 hit.
|Accession||Primary (citable) accession number: P03338|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|