P03333 (GAG_MLVAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gag polyprotein

Cleaved into the following 3 chains:

1. Matrix protein p15
   Short name=MA
2. RNA-binding phosphoprotein p12
3. Capsid protein p30
   Short name=CA

Gene names

Name:

gag

Organism

Abelson murine leukemia virus

Taxonomic identifier

11788 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus

Virus host

Mus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity. Capsid protein p30 forms the spherical core of the virus that encapsulates the genomic RNA-nucleocapsid complex By similarity.
Subcellular location	Matrix protein p15: Virion Potential. Capsid protein p30: Virion Potential.
Domain	Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. Inner coat protein p12 contains one L domain: a PPPY motif which probably binds to the WW domains of HECT (homologous to E6-AP C-terminus) E3 ubiquitin ligases By similarity.
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins.
Miscellaneous	This protein is synthesized as a Gag-Abl polyprotein.

Ontologies

Keywords
Cellular component	Virion
Molecular function	Capsid protein
PTM	Lipoprotein Myristate
Gene Ontology (GO)
Cellular component	viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed; by host By similarity
	Chain	2 – 131	130	Matrix protein p15		PRO_0000040873
	Chain	132 – 215	84	RNA-binding phosphoprotein p12		PRO_0000040874
	Chain	216 – 235	20	Capsid protein p30		PRO_0000040875
Regions
	Motif	162 – 165	4	PPPY motif
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine; by host By similarity

Sequences

	Sequence		Length	Mass (Da)
	P03333 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 4D83F71D7E056C7D	FASTA	235	25,641
	10 20 30 40 50 60 MGQTVTTPLS LTLGHWKDVE RIAHNQSVDV KKRRWVTFCS AEWPTFNVGW PRDGTFNRDL 70 80 90 100 110 120 ITQVKIKVFS PGPHGHPDQV PYIVTWEALA FDPPPWVKPF VHPKPPPPLP PSAPSLPLEP 130 140 150 160 170 180 PLSTPPRSSL YPALTPSLGA KPKPQVLSDS GGPLIDLLTE DPPPYRDPRP PPSDRDGNGG 190 200 210 220 230 EATPAGEAPD PSPMASRLRG RREPPVADST TSQAFPLRTG GNGQLQYWPF SSSDL

References

[1]

"Nucleotide sequence of Abelson murine leukemia virus genome: structural similarity of its transforming gene product to other onc gene products with tyrosine-specific kinase activity." Reddy E.P., Smith M.J., Srinivasan A. Proc. Natl. Acad. Sci. U.S.A. 80:3623-3627(1983) [PubMed: 6304726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	V01541 Genomic DNA. Translation: CAA24781.1.
3D structure databases
ProteinModelPortal	P03333.
SMR	P03333. Positions 2-98.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000840. G_retro_matrix_N. IPR002079. Gag_p12. IPR010999. Retrovr_matrix_N. [Graphical view]
Gene3D	G3DSA:1.10.150.180. G_retro_matrix_N. 1 hit.
Pfam	PF01140. Gag_MA. 1 hit. PF01141. Gag_p12. 1 hit. [Graphical view]
SUPFAM	SSF47836. Retrovir_matrix. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GAG_MLVAB

Accession

Primary (citable) accession number: P03333

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	February 8, 2011
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program