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P03333 (GAG_MLVAB) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Gag polyprotein

Cleaved into the following 3 chains:

  1. Matrix protein p15
    Short name=MA
  2. RNA-binding phosphoprotein p12
  3. Capsid protein p30
    Short name=CA
Gene names
Name:gag
OrganismAbelson murine leukemia virus
Taxonomic identifier11788 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
Virus hostMus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length235 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.

Capsid protein p30 forms the spherical core of the virus that encapsulates the genomic RNA-nucleocapsid complex By similarity.

Subcellular location

Matrix protein p15: Virion Potential.

Capsid protein p30: Virion Potential.

Domain

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Gag-p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase Potential.

Post-translational modification

Specific enzymatic cleavages in vivo yield mature proteins.

Miscellaneous

This protein is synthesized as a Gag-Abl polyprotein.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed; by host By similarity
Chain2 – 131130Matrix protein p15
PRO_0000040873
Chain132 – 21584RNA-binding phosphoprotein p12
PRO_0000040874
Chain216 – 23520Capsid protein p30
PRO_0000040875

Regions

Motif162 – 1654PPPY motif Potential
Motif162 – 1654PPXY motif Potential

Amino acid modifications

Lipidation21N-myristoyl glycine; by host By similarity

Sequences

Sequence LengthMass (Da)Tools
P03333 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 4D83F71D7E056C7D

FASTA23525,641
        10         20         30         40         50         60 
MGQTVTTPLS LTLGHWKDVE RIAHNQSVDV KKRRWVTFCS AEWPTFNVGW PRDGTFNRDL 

        70         80         90        100        110        120 
ITQVKIKVFS PGPHGHPDQV PYIVTWEALA FDPPPWVKPF VHPKPPPPLP PSAPSLPLEP 

       130        140        150        160        170        180 
PLSTPPRSSL YPALTPSLGA KPKPQVLSDS GGPLIDLLTE DPPPYRDPRP PPSDRDGNGG 

       190        200        210        220        230 
EATPAGEAPD PSPMASRLRG RREPPVADST TSQAFPLRTG GNGQLQYWPF SSSDL 

« Hide

References

[1]"Nucleotide sequence of Abelson murine leukemia virus genome: structural similarity of its transforming gene product to other onc gene products with tyrosine-specific kinase activity."
Reddy E.P., Smith M.J., Srinivasan A.
Proc. Natl. Acad. Sci. U.S.A. 80:3623-3627(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
V01541 Genomic DNA. Translation: CAA24781.1.

3D structure databases

ProteinModelPortalP03333.
SMRP03333. Positions 2-98.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.150.180. 1 hit.
InterProIPR000840. G_retro_matrix_N.
IPR002079. Gag_p12.
IPR010999. Retrovr_matrix_N.
[Graphical view]
PfamPF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
[Graphical view]
SUPFAMSSF47836. SSF47836. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGAG_MLVAB
AccessionPrimary (citable) accession number: P03333
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 89 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program