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Protein

Gag polyprotein

Gene

gag

Organism
Moloney murine leukemia virus (isolate Shinnick) (MoMLV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release.1 Publication
Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity).By similarity
Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity
Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri502 – 519CCHC-typePROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

Keywords - Ligandi

Metal-binding, RNA-binding, Viral nucleoprotein, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Short name:
Pr65gag
Alternative name(s):
Core polyprotein
Cleaved into the following 4 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Capsid protein p30
Short name:
CA
Nucleocapsid protein p10
Short name:
NC-gag
Gene namesi
Name:gag
OrganismiMoloney murine leukemia virus (isolate Shinnick) (MoMLV)
Taxonomic identifieri928306 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
Proteomesi
  • UP000006625 Componenti: Genome

Subcellular locationi

Gag polyprotein :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host endosome, Host membrane, Membrane, Viral matrix protein, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi114P → A: Slight reduction in the number of virus-like particles produced. 1
Mutagenesisi137S → A: No effect on reverse transcription activity. 1 Publication1
Mutagenesisi148S → A: No effect on reverse transcription activity; when associated with A-150. 1 Publication1
Mutagenesisi150S → A: No effect on reverse transcription activity; when associated with A-148. 1 Publication1
Mutagenesisi165Y → A: Drastic reduction in the number of virus-like particles produced. 1 Publication1
Mutagenesisi192S → A: Complete loss of reverse transcription activity. 1 Publication1
Mutagenesisi192S → D: Complete loss of reverse transcription activity. 1 Publication1
Mutagenesisi196S → A: No effect on reverse transcription activity. 1 Publication1
Mutagenesisi209S → A: Strongly reduced reverse transcription activity. 1 Publication1
Mutagenesisi209S → D: Strongly reduced reverse transcription activity. 1 Publication1
Mutagenesisi212S → A: No effect on reverse transcription activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by host1 Publication
ChainiPRO_00003908152 – 538Gag polyproteinAdd BLAST537
ChainiPRO_00000409122 – 131Matrix protein p15Sequence analysisAdd BLAST130
ChainiPRO_0000040913132 – 215RNA-binding phosphoprotein p12Sequence analysisAdd BLAST84
ChainiPRO_0000040914216 – 478Capsid protein p30Sequence analysisAdd BLAST263
ChainiPRO_0000040915479 – 538Nucleocapsid protein p10Sequence analysisAdd BLAST60

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by host1 Publication1
Modified residuei192Phosphoserine; by host1 Publication1

Post-translational modificationi

Gag polyprotein is ubiquitinated by ITCH. Gag can recruit the ubiquitin ligase Itch in an L domain-independent manner to facilitate virus release via a mechanism that involves Gag ubiquitination.
Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation (By similarity).By similarity
Capsid protein p30 is sumoylated; which is required for virus replication.1 Publication
RNA-binding phosphoprotein p12 is phosphorylated on serine residues.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei131 – 132Cleavage; by viral protease p142
Sitei215 – 216Cleavage; by viral protease p142
Sitei478 – 479Cleavage; by viral protease p142

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

PTM databases

iPTMnetiP03332.

Interactioni

Subunit structurei

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers (By similarity). Capsid protein p30 interacts with mouse UBE2I and mouse PIAS4. Gag polyprotein interacts (via PPXY motif) with host NEDD4. Gag polyprotein interacts (via PSAP motif) with host TSG101. Gag polyprotein interacts (via LYPX(n)L motif) with host PDCD6IP.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Dok3Q9QZK73EBI-935477,EBI-2906753From a different organism.
Iqgap1Q9JKF117EBI-935477,EBI-644633From a different organism.

Protein-protein interaction databases

IntActiP03332. 6 interactors.
MINTiMINT-2490859.

Structurei

Secondary structure

1538
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 14Combined sources7
Helixi16 – 25Combined sources10
Helixi32 – 40Combined sources9
Helixi43 – 46Combined sources4
Helixi58 – 68Combined sources11
Helixi77 – 79Combined sources3
Helixi80 – 91Combined sources12
Turni356 – 360Combined sources5
Helixi361 – 363Combined sources3
Helixi364 – 378Combined sources15
Beta strandi494 – 496Combined sources3
Beta strandi501 – 503Combined sources3
Beta strandi505 – 507Combined sources3
Beta strandi510 – 512Combined sources3
Helixi514 – 516Combined sources3
Beta strandi518 – 520Combined sources3
Beta strandi522 – 524Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A6BNMR-B492-531[»]
1BM4NMR-A352-382[»]
1MN8X-ray1.00A/B/C/D1-99[»]
1U6PNMR-A479-534[»]
1WWDNMR-A479-534[»]
1WWENMR-A479-534[»]
1WWFNMR-A479-534[»]
1WWGNMR-A479-534[»]
ProteinModelPortaliP03332.
SMRiP03332.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03332.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni345 – 393Interaction with mouse PIAS4Add BLAST49
Regioni430 – 435Interaction with mouse UBE2I6

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili438 – 478Sequence analysisAdd BLAST41

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi111 – 114PTAP/PSAP motif4
Motifi130 – 134LYPX(n)L motif5
Motifi162 – 165PPXY motif4

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. PPXY motif is essential for virus egress. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 also contains one L domain: a LYPX(n)L motif which interacts with PDCD6IP. Both PSAP and LYPX(n)L domains might play little to no role in budding and possibly drive residual virus release.

Sequence similaritiesi

Contains 1 CCHC-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri502 – 519CCHC-typePROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Coiled coil, Zinc-finger

Family and domain databases

Gene3Di1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR000840. G_retro_matrix.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
SMARTiSM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Pr65gag (identifier: P03332-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQTVTTPLS LTLGHWKDVE RIAHNQSVDV KKRRWVTFCS AEWPTFNVGW
60 70 80 90 100
PRDGTFNRDL ITQVKIKVFS PGPHGHPDQV PYIVTWEALA FDPPPWVKPF
110 120 130 140 150
VHPKPPPPLP PSAPSLPLEP PRSTPPRSSL YPALTPSLGA KPKPQVLSDS
160 170 180 190 200
GGPLIDLLTE DPPPYRDPRP PPSDRDGNGG EATPAGEAPD PSPMASRLRG
210 220 230 240 250
RREPPVADST TSQAFPLRAG GNGQLQYWPF SSSDLYNWKN NNPSFSEDPG
260 270 280 290 300
KLTALIESVL ITHQPTWDDC QQLLGTLLTG EEKQRVLLEA RKAVRGDDGR
310 320 330 340 350
PTQLPNEVDA AFPLERPDWD YTTQAGRNHL VHYRQLLLAG LQNAGRSPTN
360 370 380 390 400
LAKVKGITQG PNESPSAFLE RLKEAYRRYT PYDPEDPGQE TNVSMSFIWQ
410 420 430 440 450
SAPDIGRKLE RLEDLKNKTL GDLVREAEKI FNKRETPEER EERIRRETEE
460 470 480 490 500
KEERRRTEDE QKEKERDRRR HREMSKLLAT VVSGQKQDRQ GGERRRSQLD
510 520 530
RDQCAYCKEK GHWAKDCPKK PRGPRGPRPQ TSLLTLDD
Length:538
Mass (Da):60,858
Last modified:January 23, 2007 - v4
Checksum:i8A7652439B464495
GO
Isoform Pr80gag (identifier: Q8UN02-1) [UniParc]FASTAAdd to basket
Also known as: GlycoGag
The sequence of this isoform can be found in the external entry Q8UN02.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by an upstream CUG initiation codon.
Length:626
Mass (Da):70,485
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02255 Genomic RNA. Translation: AAB59942.1.
AF033811 Genomic RNA. Translation: AAC82566.1.
PIRiA03930. FOMV1M.
RefSeqiNP_057934.1. NC_001501.1.

Genome annotation databases

GeneIDi1491870.
KEGGivg:1491870.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02255 Genomic RNA. Translation: AAB59942.1.
AF033811 Genomic RNA. Translation: AAC82566.1.
PIRiA03930. FOMV1M.
RefSeqiNP_057934.1. NC_001501.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A6BNMR-B492-531[»]
1BM4NMR-A352-382[»]
1MN8X-ray1.00A/B/C/D1-99[»]
1U6PNMR-A479-534[»]
1WWDNMR-A479-534[»]
1WWENMR-A479-534[»]
1WWFNMR-A479-534[»]
1WWGNMR-A479-534[»]
ProteinModelPortaliP03332.
SMRiP03332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP03332. 6 interactors.
MINTiMINT-2490859.

PTM databases

iPTMnetiP03332.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1491870.
KEGGivg:1491870.

Miscellaneous databases

EvolutionaryTraceiP03332.

Family and domain databases

Gene3Di1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR000840. G_retro_matrix.
IPR002079. Gag_p12.
IPR003036. Gag_P30.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF01140. Gag_MA. 1 hit.
PF01141. Gag_p12. 1 hit.
PF02093. Gag_p30. 1 hit.
PF00098. zf-CCHC. 1 hit.
[Graphical view]
SMARTiSM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
PROSITEiPS50158. ZF_CCHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGAG_MLVMS
AccessioniPrimary (citable) accession number: P03332
Secondary accession number(s): Q9WJP4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 134 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.