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Protein

Gag polyprotein

Gene

gag

Organism
Moloney murine leukemia virus (isolate Shinnick) (MoMLV)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Gag polyprotein: Plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably links the viral protein to the host ESCRT pathway and facilitates release.1 Publication
Matrix protein p15: Targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the pre-integration complex.1 Publication
RNA-binding phosphoprotein p12: Constituent of the pre-integration complex (PIC) which tethers the latter to mitotic chromosomes.2 Publications
Capsid protein p30: Forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity
Nucleocapsid protein p10-Gag: Involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved UCUG elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri502 – 519CCHC-typePROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Viral nucleoprotein
Biological processHost-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Viral release from host cell
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Short name:
Pr65gag
Alternative name(s):
Core polyprotein
Cleaved into the following 4 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Capsid protein p30
Short name:
CA
Gene namesi
Name:gag
OrganismiMoloney murine leukemia virus (isolate Shinnick) (MoMLV)
Taxonomic identifieri928306 [NCBI]
Taxonomic lineageiVirusesOrterviralesRetroviridaeOrthoretrovirinaeGammaretrovirusMurine leukemia virus
Virus hostiMus musculus (Mouse) [TaxID: 10090]
Proteomesi
  • UP000006625 Componenti: Genome
  • UP000180702 Componenti: Genome

Subcellular locationi

Gag polyprotein :
RNA-binding phosphoprotein p12 :

GO - Cellular componenti

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host cytoplasm, Host endosome, Host membrane, Membrane, Viral matrix protein, Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi114P → A: Slight reduction in the number of virus-like particles produced. 1
Mutagenesisi137S → A: No effect on reverse transcription activity. 1 Publication1
Mutagenesisi148S → A: No effect on reverse transcription activity; when associated with A-150. 1 Publication1
Mutagenesisi150S → A: No effect on reverse transcription activity; when associated with A-148. 1 Publication1
Mutagenesisi165Y → A: Drastic reduction in the number of virus-like particles produced. 1 Publication1
Mutagenesisi192S → A: Complete loss of reverse transcription activity. 1 Publication1
Mutagenesisi192S → A: Complete loss of stable anchoring of viral PIC to mitotic chromosomes; when associated with A-196. 1 Publication1
Mutagenesisi192S → D: Complete loss of reverse transcription activity. 1 Publication1
Mutagenesisi196S → A: Complete loss of stable anchoring of viral PIC to mitotic chromosomes; when associated with A-192. 1 Publication1
Mutagenesisi196S → A: No effect on reverse transcription activity. 1 Publication1
Mutagenesisi209S → A: Strongly reduced reverse transcription activity. 1 Publication1
Mutagenesisi209S → D: Strongly reduced reverse transcription activity. 1 Publication1
Mutagenesisi212S → A: No effect on reverse transcription activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostSequence analysis1 Publication
ChainiPRO_00003908152 – 538Gag polyproteinAdd BLAST537
ChainiPRO_00000409122 – 131Matrix protein p15Add BLAST130
ChainiPRO_0000040913132 – 215RNA-binding phosphoprotein p12Add BLAST84
ChainiPRO_0000040914216 – 478Capsid protein p30Add BLAST263
ChainiPRO_0000040915479 – 538Nucleocapsid protein p10-GagAdd BLAST60

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by hostSequence analysis1 Publication1
Modified residuei192Phosphoserine; by host1 Publication1

Post-translational modificationi

Gag polyprotein: Ubiquitinated by ITCH. Gag can recruit the ubiquitin ligase Itch in an L domain-independent manner to facilitate virus release via a mechanism that involves Gag ubiquitination.1 Publication
Gag polyprotein: Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation.1 Publication
Capsid protein p30: Sumoylated; required for virus replication.1 Publication
RNA-binding phosphoprotein p12 is phosphorylated on serine residues.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei131 – 132Cleavage; by viral protease1 Publication2
Sitei215 – 216Cleavage; by viral protease1 Publication2
Sitei478 – 479Cleavage; by viral protease1 Publication2

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

PTM databases

iPTMnetiP03332

Interactioni

Subunit structurei

Capsid protein p30: Homohexamer; further associates as homomultimer (By similarity). Capsid protein p30: The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers (PubMed:12093170). Capsid protein p30: Interacts with mouse UBE2I and mouse PIAS4. Gag polyprotein: Interacts (via PPXY motif) with host NEDD4 (PubMed:15908698). Gag polyprotein: Interacts (via PSAP motif) with host TSG101 (PubMed:15908698). Gag polyprotein: Interacts (via LYPX(n)L motif) with host PDCD6IP (PubMed:15908698).By similarity3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

IntActiP03332, 6 interactors
MINTiP03332

Structurei

Secondary structure

1538
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 14Combined sources7
Helixi16 – 25Combined sources10
Helixi32 – 40Combined sources9
Helixi43 – 46Combined sources4
Helixi58 – 68Combined sources11
Helixi77 – 79Combined sources3
Helixi80 – 91Combined sources12
Turni356 – 360Combined sources5
Helixi361 – 363Combined sources3
Helixi364 – 378Combined sources15
Beta strandi494 – 496Combined sources3
Beta strandi501 – 503Combined sources3
Beta strandi505 – 507Combined sources3
Beta strandi510 – 512Combined sources3
Helixi514 – 516Combined sources3
Beta strandi518 – 520Combined sources3
Beta strandi522 – 524Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A6BNMR-B492-531[»]
1BM4NMR-A352-382[»]
1MN8X-ray1.00A/B/C/D1-99[»]
1U6PNMR-A479-534[»]
1WWDNMR-A479-534[»]
1WWENMR-A479-534[»]
1WWFNMR-A479-534[»]
1WWGNMR-A479-534[»]
ProteinModelPortaliP03332
SMRiP03332
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03332

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni345 – 393Interaction with host PIAS41 PublicationAdd BLAST49
Regioni430 – 435Interaction with host UBE2I1 Publication6

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili438 – 478Sequence analysisAdd BLAST41

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi111 – 114PTAP/PSAP motif1 Publication4
Motifi130 – 134LYPX(n)L motif1 Publication5
Motifi162 – 165PPXY motif1 Publication4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi71 – 193Pro-richPROSITE-ProRule annotationAdd BLAST123

Domaini

Gag polyprotein: Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. PPXY motif is essential for virus egress. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 also contains one L domain: a LYPX(n)L motif which interacts with PDCD6IP. Both PSAP and LYPX(n)L domains might play little to no role in budding and possibly drive residual virus release.1 Publication

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri502 – 519CCHC-typePROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

OrthoDBiVOG090000NB

Family and domain databases

Gene3Di1.10.150.180, 1 hit
1.10.375.10, 1 hit
InterProiView protein in InterPro
IPR000840 G_retro_matrix
IPR036946 G_retro_matrix_sf
IPR002079 Gag_p12
IPR003036 Gag_P30
IPR008919 Retrov_capsid_N
IPR010999 Retrovr_matrix
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF01140 Gag_MA, 1 hit
PF01141 Gag_p12, 1 hit
PF02093 Gag_p30, 1 hit
PF00098 zf-CCHC, 1 hit
SMARTiView protein in SMART
SM00343 ZnF_C2HC, 1 hit
SUPFAMiSSF47836 SSF47836, 1 hit
SSF47943 SSF47943, 1 hit
SSF57756 SSF57756, 1 hit
PROSITEiView protein in PROSITE
PS50158 ZF_CCHC, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Pr65gag (identifier: P03332-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQTVTTPLS LTLGHWKDVE RIAHNQSVDV KKRRWVTFCS AEWPTFNVGW
60 70 80 90 100
PRDGTFNRDL ITQVKIKVFS PGPHGHPDQV PYIVTWEALA FDPPPWVKPF
110 120 130 140 150
VHPKPPPPLP PSAPSLPLEP PRSTPPRSSL YPALTPSLGA KPKPQVLSDS
160 170 180 190 200
GGPLIDLLTE DPPPYRDPRP PPSDRDGNGG EATPAGEAPD PSPMASRLRG
210 220 230 240 250
RREPPVADST TSQAFPLRAG GNGQLQYWPF SSSDLYNWKN NNPSFSEDPG
260 270 280 290 300
KLTALIESVL ITHQPTWDDC QQLLGTLLTG EEKQRVLLEA RKAVRGDDGR
310 320 330 340 350
PTQLPNEVDA AFPLERPDWD YTTQAGRNHL VHYRQLLLAG LQNAGRSPTN
360 370 380 390 400
LAKVKGITQG PNESPSAFLE RLKEAYRRYT PYDPEDPGQE TNVSMSFIWQ
410 420 430 440 450
SAPDIGRKLE RLEDLKNKTL GDLVREAEKI FNKRETPEER EERIRRETEE
460 470 480 490 500
KEERRRTEDE QKEKERDRRR HREMSKLLAT VVSGQKQDRQ GGERRRSQLD
510 520 530
RDQCAYCKEK GHWAKDCPKK PRGPRGPRPQ TSLLTLDD
Length:538
Mass (Da):60,858
Last modified:January 23, 2007 - v4
Checksum:i8A7652439B464495
GO
Isoform Pr80gag (identifier: P03332-2)
Also known as: GlycoGag
Sequence is not available
Note: Produced by an upstream CUG initiation codon.Curated
Length:
Mass (Da):

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02255 Genomic RNA Translation: AAB59942.1
AF033811 Genomic RNA Translation: AAC82566.1
PIRiA03930 FOMV1M
RefSeqiNP_057934.1, NC_001501.1

Genome annotation databases

GeneIDi1491870
KEGGivg:1491870

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiGAG_MLVMS
AccessioniPrimary (citable) accession number: P03332
Secondary accession number(s): Q9WJP4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 141 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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