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Protein

Gag polyprotein

Gene

gag

Organism
Friend spleen focus-forming virus (isolate 502) (FSFFV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release (By similarity).By similarity
Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei129 – 1302Cleavage; by viral proteaseBy similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural constituent of virion Source: UniProtKB-KW

GO - Biological processi

  1. viral budding via host ESCRT complex Source: UniProtKB-KW
  2. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 2 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Gene namesi
Name:gag
OrganismiFriend spleen focus-forming virus (isolate 502) (FSFFV)
Taxonomic identifieri355329 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
Virus hostiMus [TaxID: 10088]

Subcellular locationi

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. membrane Source: UniProtKB-KW
  3. viral capsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by hostBy similarity
Chaini2 – 187186Gag polyproteinPRO_0000390799Add
BLAST
Chaini2 – 129128Matrix protein p15Sequence AnalysisPRO_0000040841Add
BLAST
Chaini130 – 18758RNA-binding phosphoprotein p12Sequence AnalysisPRO_0000040842Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine; by hostBy similarity

Post-translational modificationi

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Myristate

Structurei

3D structure databases

ProteinModelPortaliP03331.
SMRiP03331. Positions 2-98.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi109 – 1124PTAP/PSAP motif
Motifi128 – 1325LYPX(n)L motif

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 contains one L domain: a LYPX(n)L motif which interacts with PDCD6IP (By similarity).By similarity

Family and domain databases

Gene3Di1.10.150.180. 1 hit.
InterProiIPR000840. G_retro_matrix.
IPR010999. Retrovr_matrix.
[Graphical view]
PfamiPF01140. Gag_MA. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03331-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGQTVTTPLS LTLEHWEDVQ RTASNQSVDV KKRRWVTFCS AEWPTFGVGW
60 70 80 90 100
PQDGTFNLDI ILQVKSKVFS PGPHGHPDQV PYIVTWEAIA YEPPPWVKPF
110 120 130 140 150
VSPKLSPSPT APILPSGPST QPPPRSALYP ALTPSIKPGP SPIMADLSLT
160 170 180
FSQKTLRRTE DRDRPPLTEM ATEKRPPPLL RFLPPLP
Length:187
Mass (Da):20,804
Last modified:January 23, 2007 - v3
Checksum:i7B0774B671E31185
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00021 Genomic RNA. Translation: AAA46486.1.
PIRiA03929. FOVW5S.
RefSeqiNP_041217.1. NC_001500.1.

Genome annotation databases

GeneIDi1491887.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K00021 Genomic RNA. Translation: AAA46486.1.
PIRiA03929. FOVW5S.
RefSeqiNP_041217.1. NC_001500.1.

3D structure databases

ProteinModelPortaliP03331.
SMRiP03331. Positions 2-98.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1491887.

Family and domain databases

Gene3Di1.10.150.180. 1 hit.
InterProiIPR000840. G_retro_matrix.
IPR010999. Retrovr_matrix.
[Graphical view]
PfamiPF01140. Gag_MA. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete nucleotide sequence of an infectious clone of Friend spleen focus-forming provirus: gp55 is an envelope fusion glycoprotein."
    Clark S.P., Mak T.W.
    Proc. Natl. Acad. Sci. U.S.A. 80:5037-5041(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiGAG_FRSF5
AccessioniPrimary (citable) accession number: P03331
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 85 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Caution

This gag polyprotein does not encode any capsid and nucleoprotein.Curated

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.