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P03331

- GAG_FRSF5

UniProt

P03331 - GAG_FRSF5

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Protein
Gag polyprotein
Gene
gag
Organism
Friend spleen focus-forming virus (isolate 502) (FSFFV)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release By similarity.
Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei129 – 1302Cleavage; by viral protease By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. structural constituent of virion Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. viral budding via host ESCRT complex Source: UniProtKB-KW
  2. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 2 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Gene namesi
Name:gag
OrganismiFriend spleen focus-forming virus (isolate 502) (FSFFV)
Taxonomic identifieri355329 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
Virus hostiMus [TaxID: 10088]

Subcellular locationi

Chain Gag polyprotein : Virion By similarity. Host cell membrane; Lipid-anchor Reviewed prediction
Chain Matrix protein p15 : Virion Reviewed prediction

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. membrane Source: UniProtKB-KW
  3. viral capsid Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by host By similarity
Chaini2 – 187186Gag polyprotein
PRO_0000390799Add
BLAST
Chaini2 – 129128Matrix protein p15 Reviewed prediction
PRO_0000040841Add
BLAST
Chaini130 – 18758RNA-binding phosphoprotein p12 Reviewed prediction
PRO_0000040842Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine; by host By similarity

Post-translational modificationi

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.

Keywords - PTMi

Lipoprotein, Myristate

Structurei

3D structure databases

ProteinModelPortaliP03331.
SMRiP03331. Positions 2-98.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi109 – 1124PTAP/PSAP motif
Motifi128 – 1325LYPX(n)L motif

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 contains one L domain: a LYPX(n)L motif which interacts with PDCD6IP By similarity.

Family and domain databases

Gene3Di1.10.150.180. 1 hit.
InterProiIPR000840. G_retro_matrix_N.
IPR010999. Retrovr_matrix_N.
[Graphical view]
PfamiPF01140. Gag_MA. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03331-1 [UniParc]FASTAAdd to Basket

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MGQTVTTPLS LTLEHWEDVQ RTASNQSVDV KKRRWVTFCS AEWPTFGVGW    50
PQDGTFNLDI ILQVKSKVFS PGPHGHPDQV PYIVTWEAIA YEPPPWVKPF 100
VSPKLSPSPT APILPSGPST QPPPRSALYP ALTPSIKPGP SPIMADLSLT 150
FSQKTLRRTE DRDRPPLTEM ATEKRPPPLL RFLPPLP 187
Length:187
Mass (Da):20,804
Last modified:January 23, 2007 - v3
Checksum:i7B0774B671E31185
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K00021 Genomic RNA. Translation: AAA46486.1.
PIRiA03929. FOVW5S.
RefSeqiNP_041217.1. NC_001500.1.

Genome annotation databases

GeneIDi1491887.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K00021 Genomic RNA. Translation: AAA46486.1 .
PIRi A03929. FOVW5S.
RefSeqi NP_041217.1. NC_001500.1.

3D structure databases

ProteinModelPortali P03331.
SMRi P03331. Positions 2-98.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1491887.

Family and domain databases

Gene3Di 1.10.150.180. 1 hit.
InterProi IPR000840. G_retro_matrix_N.
IPR010999. Retrovr_matrix_N.
[Graphical view ]
Pfami PF01140. Gag_MA. 1 hit.
[Graphical view ]
SUPFAMi SSF47836. SSF47836. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete nucleotide sequence of an infectious clone of Friend spleen focus-forming provirus: gp55 is an envelope fusion glycoprotein."
    Clark S.P., Mak T.W.
    Proc. Natl. Acad. Sci. U.S.A. 80:5037-5041(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiGAG_FRSF5
AccessioniPrimary (citable) accession number: P03331
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 82 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Caution

This gag polyprotein does not encode any capsid and nucleoprotein.

External Data

Dasty 3

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