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P03331

- GAG_FRSF5

UniProt

P03331 - GAG_FRSF5

Protein

Gag polyprotein

Gene

gag

Organism
Friend spleen focus-forming virus (isolate 502) (FSFFV)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release By similarity.By similarity
    Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei129 – 1302Cleavage; by viral proteaseBy similarity

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW
    2. structural constituent of virion Source: UniProtKB-KW

    GO - Biological processi

    1. viral budding via host ESCRT complex Source: UniProtKB-KW
    2. viral release from host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gag polyprotein
    Alternative name(s):
    Core polyprotein
    Cleaved into the following 2 chains:
    Matrix protein p15
    Short name:
    MA
    Alternative name(s):
    pp12
    Gene namesi
    Name:gag
    OrganismiFriend spleen focus-forming virus (isolate 502) (FSFFV)
    Taxonomic identifieri355329 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
    Virus hostiMus [TaxID: 10088]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-KW
    3. viral capsid Source: InterPro

    Keywords - Cellular componenti

    Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed; by hostBy similarity
    Chaini2 – 187186Gag polyproteinPRO_0000390799Add
    BLAST
    Chaini2 – 129128Matrix protein p15Sequence AnalysisPRO_0000040841Add
    BLAST
    Chaini130 – 18758RNA-binding phosphoprotein p12Sequence AnalysisPRO_0000040842Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycine; by hostBy similarity

    Post-translational modificationi

    Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.By similarity

    Keywords - PTMi

    Lipoprotein, Myristate

    Structurei

    3D structure databases

    ProteinModelPortaliP03331.
    SMRiP03331. Positions 2-98.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi109 – 1124PTAP/PSAP motif
    Motifi128 – 1325LYPX(n)L motif

    Domaini

    Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 contains one L domain: a LYPX(n)L motif which interacts with PDCD6IP By similarity.By similarity

    Family and domain databases

    Gene3Di1.10.150.180. 1 hit.
    InterProiIPR000840. G_retro_matrix_N.
    IPR010999. Retrovr_matrix_N.
    [Graphical view]
    PfamiPF01140. Gag_MA. 1 hit.
    [Graphical view]
    SUPFAMiSSF47836. SSF47836. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P03331-1 [UniParc]FASTAAdd to Basket

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    MGQTVTTPLS LTLEHWEDVQ RTASNQSVDV KKRRWVTFCS AEWPTFGVGW    50
    PQDGTFNLDI ILQVKSKVFS PGPHGHPDQV PYIVTWEAIA YEPPPWVKPF 100
    VSPKLSPSPT APILPSGPST QPPPRSALYP ALTPSIKPGP SPIMADLSLT 150
    FSQKTLRRTE DRDRPPLTEM ATEKRPPPLL RFLPPLP 187
    Length:187
    Mass (Da):20,804
    Last modified:January 23, 2007 - v3
    Checksum:i7B0774B671E31185
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K00021 Genomic RNA. Translation: AAA46486.1.
    PIRiA03929. FOVW5S.
    RefSeqiNP_041217.1. NC_001500.1.

    Genome annotation databases

    GeneIDi1491887.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K00021 Genomic RNA. Translation: AAA46486.1 .
    PIRi A03929. FOVW5S.
    RefSeqi NP_041217.1. NC_001500.1.

    3D structure databases

    ProteinModelPortali P03331.
    SMRi P03331. Positions 2-98.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1491887.

    Family and domain databases

    Gene3Di 1.10.150.180. 1 hit.
    InterProi IPR000840. G_retro_matrix_N.
    IPR010999. Retrovr_matrix_N.
    [Graphical view ]
    Pfami PF01140. Gag_MA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47836. SSF47836. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of an infectious clone of Friend spleen focus-forming provirus: gp55 is an envelope fusion glycoprotein."
      Clark S.P., Mak T.W.
      Proc. Natl. Acad. Sci. U.S.A. 80:5037-5041(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

    Entry informationi

    Entry nameiGAG_FRSF5
    AccessioniPrimary (citable) accession number: P03331
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 83 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Caution

    This gag polyprotein does not encode any capsid and nucleoprotein.Curated

    External Data

    Dasty 3