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P03331 (GAG_FRSF5) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein

Cleaved into the following 2 chains:

  1. Matrix protein p15
    Short name=MA
  2. RNA-binding phosphoprotein p12
    Alternative name(s):
    pp12
Gene names
Name:gag
OrganismFriend spleen focus-forming virus (isolate 502) (FSFFV)
Taxonomic identifier355329 [NCBI]
Taxonomic lineageVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirusunclassified Gammaretrovirus
Virus hostMus [TaxID: 10088]

Protein attributes

Sequence length187 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release By similarity.

Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.

Subcellular location

Gag polyprotein: Virion By similarity. Host cell membrane; Lipid-anchor Potential.

Matrix protein p15: Virion Potential.

Domain

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 contains one L domain: a LYPX(n)L motif which interacts with PDCD6IP By similarity.

Post-translational modification

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.

Caution

This gag polyprotein does not encode any capsid and nucleoprotein.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed; by host By similarity
Chain2 – 187186Gag polyprotein
PRO_0000390799
Chain2 – 129128Matrix protein p15 Potential
PRO_0000040841
Chain130 – 18758RNA-binding phosphoprotein p12 Potential
PRO_0000040842

Regions

Motif109 – 1124PTAP/PSAP motif
Motif128 – 1325LYPX(n)L motif

Sites

Site129 – 1302Cleavage; by viral protease By similarity

Amino acid modifications

Lipidation21N-myristoyl glycine; by host By similarity

Sequences

Sequence LengthMass (Da)Tools
P03331 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 7B0774B671E31185

FASTA18720,804
        10         20         30         40         50         60 
MGQTVTTPLS LTLEHWEDVQ RTASNQSVDV KKRRWVTFCS AEWPTFGVGW PQDGTFNLDI 

        70         80         90        100        110        120 
ILQVKSKVFS PGPHGHPDQV PYIVTWEAIA YEPPPWVKPF VSPKLSPSPT APILPSGPST 

       130        140        150        160        170        180 
QPPPRSALYP ALTPSIKPGP SPIMADLSLT FSQKTLRRTE DRDRPPLTEM ATEKRPPPLL 


RFLPPLP 

« Hide

References

[1]"Complete nucleotide sequence of an infectious clone of Friend spleen focus-forming provirus: gp55 is an envelope fusion glycoprotein."
Clark S.P., Mak T.W.
Proc. Natl. Acad. Sci. U.S.A. 80:5037-5041(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K00021 Genomic RNA. Translation: AAA46486.1.
PIRFOVW5S. A03929.
RefSeqNP_041217.1. NC_001500.1.

3D structure databases

ProteinModelPortalP03331.
SMRP03331. Positions 2-98.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1491887.

Family and domain databases

Gene3D1.10.150.180. 1 hit.
InterProIPR000840. G_retro_matrix_N.
IPR010999. Retrovr_matrix_N.
[Graphical view]
PfamPF01140. Gag_MA. 1 hit.
[Graphical view]
SUPFAMSSF47836. SSF47836. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGAG_FRSF5
AccessionPrimary (citable) accession number: P03331
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 82 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program