P03331 (GAG_FRSF5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gag polyprotein

Alternative name(s):
Core polyprotein

Cleaved into the following 2 chains:

1. Matrix protein p15
   Short name=MA
2. RNA-binding phosphoprotein p12
   Alternative name(s):
   pp12

Gene names

Name:

gag

Organism

Friend spleen focus-forming virus (isolate 502) (FSFFV)

Taxonomic identifier

355329 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus › unclassified Gammaretrovirus › Spleen focus-forming virus › Friend spleen focus-forming virus

Virus host

Mus [TaxID: 10088]

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release By similarity. Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.
Subcellular location	Gag polyprotein: Virion By similarity. Host cell membrane; Lipid-anchor Potential. Matrix protein p15: Virion Potential.
Domain	Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101. The junction between the matrix protein p15 and RNA-binding phosphoprotein p12 contains one L domain: a LYPX(n)L motif which interacts with PDCD6IP By similarity.
Post-translational modification	Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.
Caution	This gag polyprotein does not encode any capsid and nucleoprotein.

Ontologies

Keywords
Biological process	Host-virus interaction Viral budding Viral budding via the host ESCRT complexes Virus exit from host cell
Cellular component	Host cell membrane Host membrane Membrane Viral matrix protein Virion
Ligand	RNA-binding
PTM	Lipoprotein Myristate
Gene Ontology (GO)
Biological_process	viral release from host cell Inferred from electronic annotation. Source: UniProtKB-KW virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW viral capsid Inferred from electronic annotation. Source: InterPro
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed; by host By similarity
	Chain	2 – 187	186	Gag polyprotein		PRO_0000390799
	Chain	2 – 129	128	Matrix protein p15 Potential		PRO_0000040841
	Chain	130 – 187	58	RNA-binding phosphoprotein p12 Potential		PRO_0000040842
Regions
	Motif	109 – 112	4	PTAP/PSAP motif
	Motif	128 – 132	5	LYPX(n)L motif
Sites
	Site	129 – 130	2	Cleavage; by viral protease By similarity
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine; by host By similarity

Sequences

	Sequence		Length	Mass (Da)
	P03331 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 7B0774B671E31185	FASTA	187	20,804
	10 20 30 40 50 60 MGQTVTTPLS LTLEHWEDVQ RTASNQSVDV KKRRWVTFCS AEWPTFGVGW PQDGTFNLDI 70 80 90 100 110 120 ILQVKSKVFS PGPHGHPDQV PYIVTWEAIA YEPPPWVKPF VSPKLSPSPT APILPSGPST 130 140 150 160 170 180 QPPPRSALYP ALTPSIKPGP SPIMADLSLT FSQKTLRRTE DRDRPPLTEM ATEKRPPPLL RFLPPLP

References

[1]	"Complete nucleotide sequence of an infectious clone of Friend spleen focus-forming provirus: gp55 is an envelope fusion glycoprotein." Clark S.P., Mak T.W. Proc. Natl. Acad. Sci. U.S.A. 80:5037-5041(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	K00021 Genomic RNA. Translation: AAA46486.1.
PIR	FOVW5S. A03929.
RefSeq	NP_041217.1. NC_001500.1.
3D structure databases
ProteinModelPortal	P03331.
SMR	P03331. Positions 2-98.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1491887.
Family and domain databases
Gene3D	1.10.150.180. 1 hit.
InterPro	IPR000840. G_retro_matrix_N. IPR010999. Retrovr_matrix_N. [Graphical view]
Pfam	PF01140. Gag_MA. 1 hit. [Graphical view]
SUPFAM	SSF47836. Retrovir_matrix. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GAG_FRSF5

Accession

Primary (citable) accession number: P03331

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 80 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program