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P03330

- GAG_WMSV

UniProt

P03330 - GAG_WMSV

Protein

Gag polyprotein

Gene

gag

Organism
Woolly monkey sarcoma virus (WMSV) (Smian sarcoma-associated virus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release By similarity.By similarity
    Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.By similarity
    Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity
    Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei128 – 1292Cleavage; by viral proteaseBy similarity
    Sitei455 – 4562Cleavage; by viral proteaseBy similarity

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. structural constituent of virion Source: UniProtKB-KW
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. viral budding via host ESCRT complex Source: UniProtKB-KW
    2. viral release from host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

    Keywords - Ligandi

    Viral nucleoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Gag polyprotein
    Alternative name(s):
    Core polyprotein
    Cleaved into the following 4 chains:
    Matrix protein p15
    Short name:
    MA
    Alternative name(s):
    pp12
    Capsid protein p30
    Short name:
    CA
    Nucleocapsid protein p10
    Short name:
    NC-gag
    Gene namesi
    Name:gag
    OrganismiWoolly monkey sarcoma virus (WMSV) (Smian sarcoma-associated virus)
    Taxonomic identifieri11970 [NCBI]
    Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirus
    Virus hostiLagothrix (woolly monkeys) [TaxID: 9518]

    Subcellular locationi

    GO - Cellular componenti

    1. host cell plasma membrane Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-KW
    3. viral nucleocapsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed; by hostBy similarity
    Chaini2 – 512511Gag polyproteinPRO_0000390820Add
    BLAST
    Chaini2 – 128127Matrix protein p15Sequence AnalysisPRO_0000040960Add
    BLAST
    Chaini129 – 19668RNA-binding phosphoprotein p12Sequence AnalysisPRO_0000040961Add
    BLAST
    Chaini197 – 455259Capsid protein p30Sequence AnalysisPRO_0000040962Add
    BLAST
    Chaini456 – 51257Nucleocapsid protein p10Sequence AnalysisPRO_0000040963Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi2 – 21N-myristoyl glycine; by hostBy similarity

    Post-translational modificationi

    Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.By similarity

    Keywords - PTMi

    Lipoprotein, Myristate

    Interactioni

    Subunit structurei

    Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP03330.
    SMRiP03330. Positions 4-96, 213-334, 339-370.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi119 – 1224PTAP/PSAP motif
    Motifi140 – 1434PPXY motif

    Domaini

    Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101 By similarity.By similarity

    Family and domain databases

    Gene3Di1.10.1200.30. 1 hit.
    1.10.150.180. 1 hit.
    1.10.375.10. 1 hit.
    4.10.60.10. 1 hit.
    InterProiIPR000840. G_retro_matrix_N.
    IPR003036. Gag_P30.
    IPR008916. Retrov_capsid_C.
    IPR008919. Retrov_capsid_N.
    IPR010999. Retrovr_matrix_N.
    IPR001878. Znf_CCHC.
    [Graphical view]
    PfamiPF01140. Gag_MA. 1 hit.
    PF02093. Gag_p30. 1 hit.
    [Graphical view]
    SUPFAMiSSF47836. SSF47836. 1 hit.
    SSF47943. SSF47943. 1 hit.
    SSF57756. SSF57756. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P03330-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGQNNSTPLS LTLGHWKDVR TRAHNLSVKI RKEKWQTFCS SEWPTFGVGW    50
    PPEGTFNLSV IFAVKRIVFQ ETGGHPDQVP YIVVWQDLAQ SPPPWVPPSA 100
    KIAVVSSPEN TQGPSAGRPS APPRPPIYPA TDDLLLLSEP PPYPAALPPP 150
    LAPPAVGPAP GQAPDSSDPE GPAAGTRSRR ARSPADDSGP DSTVILPLRA 200
    IGPPAEPNGL VPLQYWPFSS ADLYNWKSNH PSFSENPAGL TGLLESLMFS 250
    HQPTWDDCQQ LLQILFTTEE RERILLEARK NVLGDNGAPT QLENLINEAF 300
    PLNRPQWDYN TAAGRELLLV YRRTLVAGLK GAARRPTNLA KVREVLQGPA 350
    EPPSVFLERL MEAYRRYTPF DPSEEGQQAA VATAFTGQSA PDIKKKLQRL 400
    EGLQDYSLQD LVREAEKVYH KRETEEERQE REKKEAEERE RRRDRRQEKN 450
    LTRILAAVVS EGGSGDRRTG NLGNRARKTP RDGRPPLDKD QCAYCKEKGH 500
    WDEEIAPACT GR 512
    Length:512
    Mass (Da):56,894
    Last modified:January 23, 2007 - v3
    Checksum:iAAD8AFA4EAED7FD6
    GO

    Sequence cautioni

    The sequence CAA24514.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01201 Genomic DNA. Translation: CAA24514.1. Different initiation.
    PIRiA03928. FOMVGS.
    RefSeqiYP_001165469.2. NC_009424.4.
    YP_003580184.1. NC_009424.4.

    Genome annotation databases

    GeneIDi5176148.
    9538325.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    V01201 Genomic DNA. Translation: CAA24514.1 . Different initiation.
    PIRi A03928. FOMVGS.
    RefSeqi YP_001165469.2. NC_009424.4.
    YP_003580184.1. NC_009424.4.

    3D structure databases

    ProteinModelPortali P03330.
    SMRi P03330. Positions 4-96, 213-334, 339-370.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 5176148.
    9538325.

    Family and domain databases

    Gene3Di 1.10.1200.30. 1 hit.
    1.10.150.180. 1 hit.
    1.10.375.10. 1 hit.
    4.10.60.10. 1 hit.
    InterProi IPR000840. G_retro_matrix_N.
    IPR003036. Gag_P30.
    IPR008916. Retrov_capsid_C.
    IPR008919. Retrov_capsid_N.
    IPR010999. Retrovr_matrix_N.
    IPR001878. Znf_CCHC.
    [Graphical view ]
    Pfami PF01140. Gag_MA. 1 hit.
    PF02093. Gag_p30. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47836. SSF47836. 1 hit.
    SSF47943. SSF47943. 1 hit.
    SSF57756. SSF57756. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the simian sarcoma virus genome: demonstration that its acquired cellular sequences encode the transforming gene product p28sis."
      Devare S.G., Reddy E.P., Law J.D., Robbins K.C., Aaronson S.A.
      Proc. Natl. Acad. Sci. U.S.A. 80:731-735(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiGAG_WMSV
    AccessioniPrimary (citable) accession number: P03330
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 85 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program