P03330 (GAG_WMSV) Reviewed, UniProtKB/Swiss-Prot
Last modified February 19, 2014. Version 84. History...
Names and origin
|Protein names||Recommended name:|
|Organism||Woolly monkey sarcoma virus (WMSV) (Smian sarcoma-associated virus)|
|Taxonomic identifier||11970 [NCBI]|
|Taxonomic lineage||Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus ›|
|Virus host||Lagothrix (woolly monkeys) [TaxID: 9518]|
|Sequence length||512 AA.|
|Sequence processing||The displayed sequence is further processed into a mature form.|
|Protein existence||Inferred from homology|
General annotation (Comments)
Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release By similarity.
Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.
Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex By similarity.
Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization By similarity.
Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers By similarity.
Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101 By similarity.
Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.
The sequence CAA24514.1 differs from that shown. Reason: Erroneous initiation.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Initiator methionine||1||1||Removed; by host By similarity|
|Chain||2 – 512||511||Gag polyprotein||PRO_0000390820|
|Chain||2 – 128||127||Matrix protein p15 Potential||PRO_0000040960|
|Chain||129 – 196||68||RNA-binding phosphoprotein p12 Potential||PRO_0000040961|
|Chain||197 – 455||259||Capsid protein p30 Potential||PRO_0000040962|
|Chain||456 – 512||57||Nucleocapsid protein p10 Potential||PRO_0000040963|
|Motif||119 – 122||4||PTAP/PSAP motif|
|Motif||140 – 143||4||PPXY motif|
|Site||128 – 129||2||Cleavage; by viral protease By similarity|
|Site||455 – 456||2||Cleavage; by viral protease By similarity|
Amino acid modifications
|Lipidation||2||1||N-myristoyl glycine; by host By similarity|
|||"Nucleotide sequence of the simian sarcoma virus genome: demonstration that its acquired cellular sequences encode the transforming gene product p28sis."|
Devare S.G., Reddy E.P., Law J.D., Robbins K.C., Aaronson S.A.
Proc. Natl. Acad. Sci. U.S.A. 80:731-735(1983) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
|V01201 Genomic DNA. Translation: CAA24514.1. Different initiation.|
|PIR||FOMVGS. A03928. |
|RefSeq||YP_001165469.2. NC_009424.4. |
3D structure databases
|SMR||P03330. Positions 4-96, 213-334, 339-370. |
Protocols and materials databases
Genome annotation databases
Family and domain databases
|Gene3D||1.10.1200.30. 1 hit. |
18.104.22.168. 1 hit.
1.10.375.10. 1 hit.
22.214.171.124. 1 hit.
|InterPro||IPR000840. G_retro_matrix_N. |
|Pfam||PF01140. Gag_MA. 1 hit. |
PF02093. Gag_p30. 1 hit.
|SUPFAM||SSF47836. SSF47836. 1 hit. |
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
|Accession||Primary (citable) accession number: P03330|
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Viral Protein Annotation Program|