SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P03330

- GAG_WMSV

UniProt

P03330 - GAG_WMSV

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Gag polyprotein

Gene
gag
Organism
Woolly monkey sarcoma virus (WMSV) (Smian sarcoma-associated virus)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release By similarity.
Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex By similarity.
Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex By similarity.
Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei128 – 1292Cleavage; by viral protease By similarity
Sitei455 – 4562Cleavage; by viral protease By similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. structural constituent of virion Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. viral budding via host ESCRT complex Source: UniProtKB-KW
  2. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

Keywords - Ligandi

Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 4 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Capsid protein p30
Short name:
CA
Nucleocapsid protein p10
Short name:
NC-gag
Gene namesi
Name:gag
OrganismiWoolly monkey sarcoma virus (WMSV) (Smian sarcoma-associated virus)
Taxonomic identifieri11970 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirus
Virus hostiLagothrix (woolly monkeys) [TaxID: 9518]

Subcellular locationi

Chain Gag polyprotein : Virion By similarity. Host cell membrane; Lipid-anchor Reviewed prediction
Chain Matrix protein p15 : Virion Reviewed prediction
Chain Capsid protein p30 : Virion Reviewed prediction

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. membrane Source: UniProtKB-KW
  3. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by host By similarity
Chaini2 – 512511Gag polyproteinPRO_0000390820Add
BLAST
Chaini2 – 128127Matrix protein p15 Reviewed predictionPRO_0000040960Add
BLAST
Chaini129 – 19668RNA-binding phosphoprotein p12 Reviewed predictionPRO_0000040961Add
BLAST
Chaini197 – 455259Capsid protein p30 Reviewed predictionPRO_0000040962Add
BLAST
Chaini456 – 51257Nucleocapsid protein p10 Reviewed predictionPRO_0000040963Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine; by host By similarity

Post-translational modificationi

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation By similarity.

Keywords - PTMi

Lipoprotein, Myristate

Interactioni

Subunit structurei

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers By similarity.

Structurei

3D structure databases

ProteinModelPortaliP03330.
SMRiP03330. Positions 4-96, 213-334, 339-370.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi119 – 1224PTAP/PSAP motif
Motifi140 – 1434PPXY motif

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101 By similarity.

Family and domain databases

Gene3Di1.10.1200.30. 1 hit.
1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR000840. G_retro_matrix_N.
IPR003036. Gag_P30.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF01140. Gag_MA. 1 hit.
PF02093. Gag_p30. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03330-1 [UniParc]FASTAAdd to Basket

« Hide

MGQNNSTPLS LTLGHWKDVR TRAHNLSVKI RKEKWQTFCS SEWPTFGVGW    50
PPEGTFNLSV IFAVKRIVFQ ETGGHPDQVP YIVVWQDLAQ SPPPWVPPSA 100
KIAVVSSPEN TQGPSAGRPS APPRPPIYPA TDDLLLLSEP PPYPAALPPP 150
LAPPAVGPAP GQAPDSSDPE GPAAGTRSRR ARSPADDSGP DSTVILPLRA 200
IGPPAEPNGL VPLQYWPFSS ADLYNWKSNH PSFSENPAGL TGLLESLMFS 250
HQPTWDDCQQ LLQILFTTEE RERILLEARK NVLGDNGAPT QLENLINEAF 300
PLNRPQWDYN TAAGRELLLV YRRTLVAGLK GAARRPTNLA KVREVLQGPA 350
EPPSVFLERL MEAYRRYTPF DPSEEGQQAA VATAFTGQSA PDIKKKLQRL 400
EGLQDYSLQD LVREAEKVYH KRETEEERQE REKKEAEERE RRRDRRQEKN 450
LTRILAAVVS EGGSGDRRTG NLGNRARKTP RDGRPPLDKD QCAYCKEKGH 500
WDEEIAPACT GR 512
Length:512
Mass (Da):56,894
Last modified:January 23, 2007 - v3
Checksum:iAAD8AFA4EAED7FD6
GO

Sequence cautioni

The sequence CAA24514.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01201 Genomic DNA. Translation: CAA24514.1. Different initiation.
PIRiA03928. FOMVGS.
RefSeqiYP_001165469.2. NC_009424.4.
YP_003580184.1. NC_009424.4.

Genome annotation databases

GeneIDi5176148.
9538325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
V01201 Genomic DNA. Translation: CAA24514.1 . Different initiation.
PIRi A03928. FOMVGS.
RefSeqi YP_001165469.2. NC_009424.4.
YP_003580184.1. NC_009424.4.

3D structure databases

ProteinModelPortali P03330.
SMRi P03330. Positions 4-96, 213-334, 339-370.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 5176148.
9538325.

Family and domain databases

Gene3Di 1.10.1200.30. 1 hit.
1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProi IPR000840. G_retro_matrix_N.
IPR003036. Gag_P30.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix_N.
IPR001878. Znf_CCHC.
[Graphical view ]
Pfami PF01140. Gag_MA. 1 hit.
PF02093. Gag_p30. 1 hit.
[Graphical view ]
SUPFAMi SSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the simian sarcoma virus genome: demonstration that its acquired cellular sequences encode the transforming gene product p28sis."
    Devare S.G., Reddy E.P., Law J.D., Robbins K.C., Aaronson S.A.
    Proc. Natl. Acad. Sci. U.S.A. 80:731-735(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGAG_WMSV
AccessioniPrimary (citable) accession number: P03330
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 84 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Similar proteinsi