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Protein

Gag polyprotein

Gene

gag

Organism
Woolly monkey sarcoma virus (WMSV) (Smian sarcoma-associated virus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Gag polyprotein plays a role in budding and is processed by the viral protease during virion maturation outside the cell. During budding, it recruits, in a PPXY-dependent or independent manner, Nedd4-like ubiquitin ligases that conjugate ubiquitin molecules to Gag, or to Gag binding host factors. Interaction with HECT ubiquitin ligases probably link the viral protein to the host ESCRT pathway and facilitate release (By similarity).By similarity
Matrix protein p15 targets Gag and gag-pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex (By similarity).By similarity
Capsid protein p30 forms the spherical core of the virion that encapsulates the genomic RNA-nucleocapsid complex.By similarity
Nucleocapsid protein p10 is involved in the packaging and encapsidation of two copies of the genome. Binds with high affinity to conserved elements within the packaging signal, located near the 5'-end of the genome. This binding is dependent on genome dimerization (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei128 – 1292Cleavage; by viral proteaseBy similarity
Sitei455 – 4562Cleavage; by viral proteaseBy similarity

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. structural constituent of virion Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. viral budding via host ESCRT complex Source: UniProtKB-KW
  2. viral release from host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral budding, Viral budding via the host ESCRT complexes, Virus exit from host cell

Keywords - Ligandi

Viral nucleoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Gag polyprotein
Alternative name(s):
Core polyprotein
Cleaved into the following 4 chains:
Matrix protein p15
Short name:
MA
Alternative name(s):
pp12
Capsid protein p30
Short name:
CA
Nucleocapsid protein p10
Short name:
NC-gag
Gene namesi
Name:gag
OrganismiWoolly monkey sarcoma virus (WMSV) (Smian sarcoma-associated virus)
Taxonomic identifieri11970 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesRetroviridaeOrthoretrovirinaeGammaretrovirus
Virus hostiLagothrix (woolly monkeys) [TaxID: 9518]

Subcellular locationi

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. membrane Source: UniProtKB-KW
  3. viral nucleocapsid Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cell membrane, Host membrane, Membrane, Viral matrix protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed; by hostBy similarity
Chaini2 – 512511Gag polyproteinPRO_0000390820Add
BLAST
Chaini2 – 128127Matrix protein p15Sequence AnalysisPRO_0000040960Add
BLAST
Chaini129 – 19668RNA-binding phosphoprotein p12Sequence AnalysisPRO_0000040961Add
BLAST
Chaini197 – 455259Capsid protein p30Sequence AnalysisPRO_0000040962Add
BLAST
Chaini456 – 51257Nucleocapsid protein p10Sequence AnalysisPRO_0000040963Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine; by hostBy similarity

Post-translational modificationi

Specific enzymatic cleavages by the viral protease yield mature proteins. The protease is released by autocatalytic cleavage. The polyprotein is cleaved during and after budding, this process is termed maturation (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Myristate

Interactioni

Subunit structurei

Capsid protein p30 is a homohexamer, that further associates as homomultimer. The virus core is composed of a lattice formed from hexagonal rings, each containing six capsid monomers (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP03330.
SMRiP03330. Positions 4-96, 213-334, 339-370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi119 – 1224PTAP/PSAP motif
Motifi140 – 1434PPXY motif

Domaini

Late-budding domains (L domains) are short sequence motifs essential for viral particle budding. They recruit proteins of the host ESCRT machinery (Endosomal Sorting Complex Required for Transport) or ESCRT-associated proteins. RNA-binding phosphoprotein p12 contains one L domain: a PPXY motif which potentially interacts with the WW domain 3 of NEDD4 E3 ubiquitin ligase. Matrix protein p15 contains one L domain: a PTAP/PSAP motif, which potentially interacts with the UEV domain of TSG101 (By similarity).By similarity

Family and domain databases

Gene3Di1.10.1200.30. 1 hit.
1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR000840. G_retro_matrix.
IPR003036. Gag_P30.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF01140. Gag_MA. 1 hit.
PF02093. Gag_p30. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03330-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQNNSTPLS LTLGHWKDVR TRAHNLSVKI RKEKWQTFCS SEWPTFGVGW
60 70 80 90 100
PPEGTFNLSV IFAVKRIVFQ ETGGHPDQVP YIVVWQDLAQ SPPPWVPPSA
110 120 130 140 150
KIAVVSSPEN TQGPSAGRPS APPRPPIYPA TDDLLLLSEP PPYPAALPPP
160 170 180 190 200
LAPPAVGPAP GQAPDSSDPE GPAAGTRSRR ARSPADDSGP DSTVILPLRA
210 220 230 240 250
IGPPAEPNGL VPLQYWPFSS ADLYNWKSNH PSFSENPAGL TGLLESLMFS
260 270 280 290 300
HQPTWDDCQQ LLQILFTTEE RERILLEARK NVLGDNGAPT QLENLINEAF
310 320 330 340 350
PLNRPQWDYN TAAGRELLLV YRRTLVAGLK GAARRPTNLA KVREVLQGPA
360 370 380 390 400
EPPSVFLERL MEAYRRYTPF DPSEEGQQAA VATAFTGQSA PDIKKKLQRL
410 420 430 440 450
EGLQDYSLQD LVREAEKVYH KRETEEERQE REKKEAEERE RRRDRRQEKN
460 470 480 490 500
LTRILAAVVS EGGSGDRRTG NLGNRARKTP RDGRPPLDKD QCAYCKEKGH
510
WDEEIAPACT GR
Length:512
Mass (Da):56,894
Last modified:January 23, 2007 - v3
Checksum:iAAD8AFA4EAED7FD6
GO

Sequence cautioni

The sequence CAA24514.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01201 Genomic DNA. Translation: CAA24514.1. Different initiation.
PIRiA03928. FOMVGS.
RefSeqiYP_001165469.2. NC_009424.4.
YP_003580184.1. NC_009424.4.

Genome annotation databases

GeneIDi5176148.
9538325.
KEGGivg:5176148.
vg:9538325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01201 Genomic DNA. Translation: CAA24514.1. Different initiation.
PIRiA03928. FOMVGS.
RefSeqiYP_001165469.2. NC_009424.4.
YP_003580184.1. NC_009424.4.

3D structure databases

ProteinModelPortaliP03330.
SMRiP03330. Positions 4-96, 213-334, 339-370.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi5176148.
9538325.
KEGGivg:5176148.
vg:9538325.

Family and domain databases

Gene3Di1.10.1200.30. 1 hit.
1.10.150.180. 1 hit.
1.10.375.10. 1 hit.
4.10.60.10. 1 hit.
InterProiIPR000840. G_retro_matrix.
IPR003036. Gag_P30.
IPR008916. Retrov_capsid_C.
IPR008919. Retrov_capsid_N.
IPR010999. Retrovr_matrix.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF01140. Gag_MA. 1 hit.
PF02093. Gag_p30. 1 hit.
[Graphical view]
SUPFAMiSSF47836. SSF47836. 1 hit.
SSF47943. SSF47943. 1 hit.
SSF57756. SSF57756. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of the simian sarcoma virus genome: demonstration that its acquired cellular sequences encode the transforming gene product p28sis."
    Devare S.G., Reddy E.P., Law J.D., Robbins K.C., Aaronson S.A.
    Proc. Natl. Acad. Sci. U.S.A. 80:731-735(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiGAG_WMSV
AccessioniPrimary (citable) accession number: P03330
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: April 1, 2015
This is version 88 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.