Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P03311 (POLG_FMDVS)

Last modified June 16, 2009. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Genome polyprotein
Cleaved into the following 6 chains:
    1- Recommended name:
            Protein VP3
        Alternative name(s):
            Virion protein 3
            P1C
    2- Recommended name:
            Protein VP1
        Alternative name(s):
            Virion protein 1
            P1D
    3- Recommended name:
            Protein 2A
                Short name=P2A
        Alternative name(s):
            P52
    4- Recommended name:
            Protein 2B
    5- Recommended name:
            Picornain 3C
              EC=3.4.22.28
        Alternative name(s):
            Protease 3C
              Short name=P3C
            Protease P20B
    6- Recommended name:
            RNA-directed RNA polymerase 3D-POL
                Short name=P3D-POL
              EC=2.7.7.48
        Alternative name(s):
            P56A
OrganismFoot-and-mouth disease virus (strain C1-Santa Pau) (Aphthovirus C) (FMDV)
Taxonomic identifier12120 [NCBI]
Taxonomic lineageViruses › ssRNA positive-strand viruses, no DNA stage › Picornavirales › Picornaviridae › Aphthovirus
Virus hostErinaceidae (hedgehogs) [TaxID: 9363]
Loxodonta africana (African elephant) [TaxID: 9785]
Sus scrofa (Pig) [TaxID: 9823]
Cervidae (deer) [TaxID: 9850]
Bos taurus (Bovine) [TaxID: 9913]
Capra hircus (Goat) [TaxID: 9925]
Ovis aries (Sheep) [TaxID: 9940]
Rattus norvegicus (Rat) [TaxID: 10116]

Hide | Top

Protein attributes

Sequence length861 AA.
Sequence statusFragments.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Protein 3C is a cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind co-operatively to the protease By similarity.

RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals By similarity.

Catalytic activity

Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subcellular location

Protein VP3: Virion. Host cytoplasm Potential.

Picornain 3C: Host cytoplasm Potential.

RNA-directed RNA polymerase 3D-POL: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side Potential. Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum By similarity.

Post-translational modification

Specific enzymatic cleavages in vivo by the viral proteases yield a variety of precursors and mature proteins By similarity.

Sequence similarities

Belongs to the picornaviruses polyprotein family.

Contains 1 peptidase C3 domain.

Contains 1 RdRp catalytic domain.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 46›46Protein VP3
PRO_0000039886
Chain47 – 252206Protein VP1 Potential
PRO_0000039887
Chain253 – 27018Protein 2A Potential
PRO_0000039888
Chain271 – ›332›62Protein 2B Potential
PRO_0000310979
Chain‹333 – 391›59Picornain 3C Potential
PRO_0000039889
Chain392 – 861470RNA-directed RNA polymerase 3D-POL Potential
PRO_0000039890

Regions

Domain625 – 743119RdRp catalytic

Sites

Active site3411For picornain 3C activity Potential
Site46 – 472Cleavage; by picornain 3C Potential
Site252 – 2532Cleavage; by picornain 3C Potential
Site270 – 2712Cleavage; by ribosomal skip Potential
Site391 – 3922Cleavage; by picornain 3C Potential

Experimental info

Non-adjacent residues332 – 3332
Non-terminal residue11

Secondary structure

1 ....... 861
Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P03311-1 [UniParc].

Last modified November 1, 1988. Version 1.
Checksum: 507CD16E61C893D1

FASTA86195,556
        10         20         30         40         50         60 
AETTCVQGWV CVYQITHGKA EPTRSSSRIX AGKDFELRLP VDARQQTTTT GESADPVTTT 

        70         80         90        100        110        120 
VENYGGETQV QRRHHTDVAF VLDRFVKVTV SDNQHTLDVM QAHKDNIVGA LSRHTYYFSD 

       130        140        150        160        170        180 
LEIAVTHTGK LTWVPNGAPV SALNNTTNPT AYHKGPVTRL ALPYTAPHRV LATAYTGTTT 

       190        200        210        220        230        240 
YTASARGDLA HLTTTHARHL PTSFNFGAVK AETITELLVR MKRAELYCPR PILPIQPTGD 

       250        260        270        280        290        300 
RHKQPLVAPA KQLLNFDLLK LAGDVESNLG PFFFSDVRSN FSKLVETINQ MQEDMSTKHE 

       310        320        330        340        350        360 
PDFNRLVSAF EELASGVKAI RTGLDEAKPY KLKAATKVGY CGGAVLAKDG ADTFIVGTHS 

       370        380        390        400        410        420 
AGGNGVGYCS CVSRSMLLKM KAHIDPEPHH EGLIVDTRDV EERVHVMRKT KLAPTVAHGV 

       430        440        450        460        470        480 
FNPEFGPAAL SNKDPRLNEG VVLDEVIFSR HKGDTKMSAE DKALFRRCAA DYASRLHSVL 

       490        500        510        520        530        540 
GTANAPLSIY EVIKGVDGLD AMEPDTAPSL PWALQGKRRG ALIDFENGTV GPEAEAALKL 

       550        560        570        580        590        600 
MEKREYKFAC QTFLKDEIRP MEKVRAGKTR IVDVLPVEHI LYTRMMIGRF CAQMHSNNGP 

       610        620        630        640        650        660 
QIGSAVGCNP DVDRQRSGTH LAQYRNVWDV DYSAFDANHC SDAMNIMFEE VFRTEFGFHP 

       670        680        690        700        710        720 
NAEWILKTLV NTEYAYENKR ITVEGGMPSG CSATSIVNTI LNNIYVLYAL RRHYEGVELD 

       730        740        750        760        770        780 
TYTMISYGDD IVVASDYDLD FEALKPHFKS LGQTITPADK SDKGFVLGHS ITDVTFLKRH 

       790        800        810        820        830        840 
FHMDYGTGFY KPVMTSKTLE AILSFARRGT IQEKLISVAG LAVHSGPDEY RRFFEPFQGL 

       850        860 
FEIPSYRSLY LRWVNAVCGR A

« Hide

Hide | Top

References

[1]"Molecular cloning of cDNA from foot-and-mouth disease virus C1-Santa Pau (C-S8). Sequence of protein-VP1-coding segment."
Villanueva N., Davila M., Ortin J., Domingo E.
Gene 23:185-194(1983) [PubMed: 6311686] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-332.
[2]"Sequence of the viral replicase gene from foot-and-mouth disease virus C1-Santa Pau (C-S8)."
Martinez-Salas E., Ortin J., Domingo E.
Gene 35:55-61(1985) [PubMed: 2993105] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 333-861.
[3]"Structure of the complex of an Fab fragment of a neutralizing antibody with foot-and-mouth disease virus: positioning of a highly mobile antigenic loop."
Hewat E.A., Verdaguer N., Fita I., Blakemore W., Brookes S., King A., Newman J., Domingo E., Mateu M.G., Stuart D.I.
EMBO J. 16:1492-1500(1997) [PubMed: 9130694] [Abstract]
Cited for: STRUCTURE BY ELECTRON MICROSCOPY (30.0 ANGSTROMS) OF 178-201.

Hide | Top

Cross-references

Sequence databases

M11027 Genomic RNA. Translation: AAA42654.1.
PIRA03913.
A24031.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1QGCelectron microscopy30.005178-201[»]
SMRP03311. Positions 1-46, 47-252, 392-861.
ModBaseSearch...

Protein family/group databases

MEROPSC03.008.

Family and domain databases

InterProIPR004080. FMDV_VP1_coat.
IPR000199. Pept_C3_picorn.
IPR001676. Picornavirus_capsid.
IPR001205. RNA_pol_P3D.
IPR007094. RNA_pol_PSvir.
[Graphical view]
PfamPF00548. Peptidase_C3. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 1 hit.
[Graphical view]
PRINTSPR01542. FMDVP1COAT.
PROSITEPS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry namePOLG_FMDVS
AccessionPrimary (citable) accession number: P03311
Secondary accession number(s): Q84781, Q84782
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: November 1, 1988
Last modified: June 16, 2009
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectVirus (Virus annotation project)

Hide | Top

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents