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Protein

Core protein VP8

Gene

VACWR091

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Major core structural protein.

GO - Molecular functioni

Names & Taxonomyi

Protein namesi
Recommended name:
Core protein VP8
Alternative name(s):
25 kDa major core protein
F5 polypeptide
L4 core protein
P25K
Gene namesi
Ordered Locus Names:VACWR091
ORF Names:L4R
OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Taxonomic identifieri10254 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Proteomesi
  • UP000000344 Componenti: Genome

Subcellular locationi

  • Virion 1 Publication
  • Note: Localizes to the virion core.

GO - Cellular componenti

Keywords - Cellular componenti

Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi18G → A: No effect on cleavage by core protease I7. 1 Publication1
Mutagenesisi32G → A: Complete loss of cleavage by core protease I7. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000405911 – 32Removed by core protease I72 PublicationsAdd BLAST32
ChainiPRO_000004059233 – 251Core protein VP8Add BLAST219

Post-translational modificationi

Undergoes morphogenesis-associated proteolysis which cleaves the 28 kDa to a 25-kDa product. Proteolytic cleavage of major core proteins P4a (A10L), P4b (A3L), and VP8 (L4R), which occurs at a late stage of core formation, is required for production of infectious mature virions (MV).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei18 – 19Cleavage; by core protease I7Curated2
Sitei32 – 33Cleavage; by core protease I72

Miscellaneous databases

PMAP-CutDBiP03295.

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.1 Publication

Keywords - Developmental stagei

Late protein

Interactioni

Protein-protein interaction databases

DIPiDIP-2168N.
IntActiP03295. 1 interactor.
MINTiMINT-131190.

Family & Domainsi

Sequence similaritiesi

Belongs to the chordopoxvirinae VP8 family.Curated

Phylogenomic databases

OrthoDBiVOG090000OK.

Family and domain databases

InterProiView protein in InterPro
IPR007586. VP8_pox_nuc-bd.
PfamiView protein in Pfam
PF04498. Pox_VP8_L4R. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD011473. VP8_pox_nuc_bd. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P03295-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLLENLIE EDTIFFAGSI SEYDDLQMVI AGAKSKFPRS MLSIFNIVPR
60 70 80 90 100
TMSKYELELI HNENITGAMF TTMYNIRNNL GLGDDKLTIE AIENYFLDPN
110 120 130 140 150
NEVMPLIINN TDMTAVIPKK SGRRKNKNMV IFRQGSSPIL CIFETRKKIN
160 170 180 190 200
IYKENMESAS TEYTPIGDNK ALISKYAGIN ILNVYSPSTS IRLNAIYGFT
210 220 230 240 250
NKNKLEKLST NKELESYSSS PLQEPIRLND FLGLLECVKK NIPLTDIPTK

D
Length:251
Mass (Da):28,457
Last modified:April 1, 1988 - v1
Checksum:i7FEC9BAFFFC45737
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144E → Q in AAA48232 (PubMed:3903212).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X01978 Genomic DNA. Translation: CAA26013.1.
M11758 Genomic DNA. Translation: AAA48232.1.
AY243312 Genomic DNA. Translation: AAO89370.1.
PIRiA03867. WMVZ28.
D23092. WMVZF5.
RefSeqiYP_232973.1. NC_006998.1.

Genome annotation databases

GeneIDi3707547.
KEGGivg:3707547.

Similar proteinsi

Entry informationi

Entry nameiVP8_VACCW
AccessioniPrimary (citable) accession number: P03295
Secondary accession number(s): Q76ZT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 1, 1988
Last modified: March 15, 2017
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families