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P03281

- CAP9_ADE05

UniProt

P03281 - CAP9_ADE05

Protein

Hexon-interlacing protein

Gene

IX

Organism
Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Structural component of the virion that presumably stabilizes the groups of hexons but is dispensable for assembly. During virus entry, recruits the anterograde motor kinesin-1 to the capsid docked at the nuclear pore complex thereby subjecting the docked capsid to a pulling force. The resulting tension leads to capsid disruption, dispersion of capsid fragments toward cell periphery and eventually viral DNA entry into the host nucleus.1 Publication

    GO - Biological processi

    1. viral entry into host cell Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction, Virus entry into host cell

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hexon-interlacing protein
    Alternative name(s):
    Protein IX
    Gene namesi
    ORF Names:IX
    OrganismiHuman adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
    Taxonomic identifieri28285 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000004992: Genome

    Subcellular locationi

    Virion 1 Publication. Host nucleus 1 Publication
    Note: Located in the canyons between the hexons on the outer surface of the capsid. Forms a sort of hairnet on the outer side of the virion. Present in 240 copies per virion.

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell
    2. viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, Host nucleus, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 140140Hexon-interlacing proteinPRO_0000221846Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei135 – 1351Phosphoserine; by hostBy similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Inductioni

    Expressed in the intermediate phase of the viral replicative cycle.

    Interactioni

    Subunit structurei

    Homotrimer. Interacts with hexon protein; this interaction tethers the hexons together. Self-interacts with adjacent proteins. Interacts with kinesin light chain KLC1; this interaction leads to capsid disruption at the nuclear pore complex during virus entry into host cell.1 Publication

    Protein-protein interaction databases

    DIPiDIP-29895N.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3IYNelectron microscopy-Q/R/S/T1-140[»]
    ProteinModelPortaliP03281.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03281.

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili97 – 13337Sequence AnalysisAdd
    BLAST

    Domaini

    Three N-terminal domains of hexon-interlacing protein form triskelions between hexon capsomers.

    Sequence similaritiesi

    Keywords - Domaini

    Coiled coil

    Family and domain databases

    InterProiIPR005641. Hexon_assoc_IX.
    [Graphical view]
    PfamiPF03955. Adeno_PIX. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P03281-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTNSFDGSI VSSYLTTRMP PWAGVRQNVM GSSIDGRPVL PANSTTLTYE    50
    TVSGTPLETA ASAAASAAAA TARGIVTDFA FLSPLASSAA SRSSARDDKL 100
    TALLAQLDSL TRELNVVSQQ LLDLRQQVSA LKASSPPNAV 140
    Length:140
    Mass (Da):14,458
    Last modified:July 21, 1986 - v1
    Checksum:iDAFD4045CAB616FB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73260 Genomic DNA. No translation available.
    X02996 Genomic DNA. Translation: CAA26746.1.
    PIRiA03853. SXAD95.
    RefSeqiAP_000200.1. AC_000008.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73260 Genomic DNA. No translation available.
    X02996 Genomic DNA. Translation: CAA26746.1 .
    PIRi A03853. SXAD95.
    RefSeqi AP_000200.1. AC_000008.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3IYN electron microscopy - Q/R/S/T 1-140 [» ]
    ProteinModelPortali P03281.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29895N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P03281.

    Family and domain databases

    InterProi IPR005641. Hexon_assoc_IX.
    [Graphical view ]
    Pfami PF03955. Adeno_PIX. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The 2.2 kb E1b mRNA of human Ad12 and Ad5 codes for two tumor antigens starting at different AUG triplets."
      Bos J.L., Polder L.J., Bernards R., Schrier P.I., van den Elsen P.J., van der Eb A.J., van Ormondt H.
      Cell 27:121-131(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of adenovirus type 5 early region E1: the region between map positions 8.0 (HindIII site) and 11.8 (SmaI site)."
      Maat J., van Beveren C.P., van Ormondt H.
      Gene 10:27-38(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The sequence of the genome of adenovirus type 5 and its comparison with the genome of adenovirus type 2."
      Chroboczek J., Bieber F., Jacrot B.
      Virology 186:280-285(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "De novo derivation of proteomes from transcriptomes for transcript and protein identification."
      Evans V.C., Barker G., Heesom K.J., Fan J., Bessant C., Matthews D.A.
      Nat. Methods 9:1207-1211(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    5. "Atomic structure of human adenovirus by cryo-EM reveals interactions among protein networks."
      Liu H., Jin L., Koh S.B., Atanasov I., Schein S., Wu L., Zhou Z.H.
      Science 329:1038-1043(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY ELECTRON MICROSCOPY (3.6 ANGSTROMS) OF THE VIRAL PARTICLE, FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH HEXON PROTEIN.
    6. "Latest insights on adenovirus structure and assembly."
      San Martin C.
      Viruses 4:847-877(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry nameiCAP9_ADE05
    AccessioniPrimary (citable) accession number: P03281
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This protein is only encoded by mastadenoviruses, and may therefore play a role in mammals tropism.

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3