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Protein

Penton protein

Gene

L2

Organism
Human adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Major capsid protein that self-associates to form penton base pentamers, each in the shape of a pentagon, situated at the 12 vertices of the pseudo T=25 capsid. Involved in virus secondary attachment to host cell after initial attachment by the fiber protein. Binds host integrin heterodimer ITGAV-ITGB5 (alphaV-beta5) thereby triggering clathrin-mediated endocytosis of virions. Mediates initial virus attachment to CXADR-negative cells. Binding to integrins ITGAV-ITGB5 also seems to induce macropinocytosis uptake of the virus. As the virus enters the host cell, penton proteins are shed concomitant with virion acidification in the endosome.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Clathrin-mediated endocytosis of virus by host, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Penton protein
Short name:
CP-P
Alternative name(s):
Penton base protein
Protein III
Gene namesi
ORF Names:L2
OrganismiHuman adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Taxonomic identifieri10515 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008167 Componenti: Genome

Subcellular locationi

  • Virion 1 Publication
  • Host nucleus 1 Publication

  • Note: Located at each vertex of the virion. Present in 60 copies per virion.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host nucleus, T=25 icosahedral capsid protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002218721 – 571Penton proteinAdd BLAST571

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei455Phosphoserine; by host1 Publication1

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiP03276.

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts with the fiber protein (via N-terminal tail region) (Probable). Interacts with the capsid vertex protein; this interaction binds the penton base to neighboring peripentonal hexons. Interacts (via the cell attachment site RGD) with host heterodimer ITGAV-ITGB5; this interaction promotes virus internalization. Interacts with host WWP1 and WWP2.Curated4 Publications

Structurei

Secondary structure

1571
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni50 – 52Combined sources3
Beta strandi55 – 57Combined sources3
Beta strandi58 – 60Combined sources3
Beta strandi69 – 73Combined sources5
Helixi76 – 81Combined sources6
Beta strandi82 – 85Combined sources4
Beta strandi90 – 94Combined sources5
Beta strandi99 – 101Combined sources3
Helixi103 – 106Combined sources4
Beta strandi110 – 113Combined sources4
Beta strandi117 – 129Combined sources13
Turni136 – 139Combined sources4
Beta strandi141 – 152Combined sources12
Beta strandi155 – 158Combined sources4
Beta strandi160 – 169Combined sources10
Helixi177 – 194Combined sources18
Turni195 – 200Combined sources6
Helixi203 – 205Combined sources3
Beta strandi209 – 213Combined sources5
Beta strandi217 – 220Combined sources4
Turni221 – 224Combined sources4
Beta strandi235 – 242Combined sources8
Beta strandi247 – 249Combined sources3
Helixi255 – 259Combined sources5
Beta strandi261 – 265Combined sources5
Beta strandi267 – 269Combined sources3
Beta strandi272 – 274Combined sources3
Helixi275 – 277Combined sources3
Beta strandi282 – 284Combined sources3
Helixi289 – 294Combined sources6
Turni349 – 353Combined sources5
Turni386 – 388Combined sources3
Beta strandi393 – 397Combined sources5
Beta strandi399 – 405Combined sources7
Helixi406 – 412Combined sources7
Turni415 – 417Combined sources3
Helixi419 – 422Combined sources4
Beta strandi423 – 425Combined sources3
Beta strandi436 – 439Combined sources4
Turni442 – 444Combined sources3
Helixi457 – 459Combined sources3
Beta strandi464 – 467Combined sources4
Beta strandi469 – 475Combined sources7
Helixi479 – 482Combined sources4
Helixi483 – 485Combined sources3
Beta strandi487 – 489Combined sources3
Turni495 – 497Combined sources3
Beta strandi503 – 506Combined sources4
Beta strandi512 – 519Combined sources8
Beta strandi522 – 531Combined sources10
Beta strandi533 – 542Combined sources10
Beta strandi554 – 568Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X9PX-ray3.30A49-571[»]
1X9TX-ray3.50A49-571[»]
2C6SX-ray3.60A/B/C/D/E/F/G/H/I/J/K/L/M/N/O49-571[»]
2C9Felectron microscopy16.50A/B/C/D/E49-571[»]
2C9Gelectron microscopy9.30A/B/C/D/E49-571[»]
4V4Uelectron microscopy10.00A/B/C/D/E49-571[»]
ProteinModelPortaliP03276.
SMRiP03276.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03276.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni336 – 348DisorderedSequence analysisAdd BLAST13

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi340 – 342Cell attachment siteSequence analysis3

Domaini

The cell attachment RGD motif is exposed at the virion surface and is involved in binding to the integrin heterodimer ITGAV-ITGB5.

Sequence similaritiesi

Belongs to the adenoviridae penton family.Curated

Family and domain databases

InterProiIPR002605. Adeno_Penton_B.
[Graphical view]
PfamiPF01686. Adeno_Penton_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P03276-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQRAAMYEEG PPPSYESVVS AAPVAAALGS PFDAPLDPPF VPPRYLRPTG
60 70 80 90 100
GRNSIRYSEL APLFDTTRVY LVDNKSTDVA SLNYQNDHSN FLTTVIQNND
110 120 130 140 150
YSPGEASTQT INLDDRSHWG GDLKTILHTN MPNVNEFMFT NKFKARVMVS
160 170 180 190 200
RSLTKDKQVE LKYEWVEFTL PEGNYSETMT IDLMNNAIVE HYLKVGRQNG
210 220 230 240 250
VLESDIGVKF DTRNFRLGFD PVTGLVMPGV YTNEAFHPDI ILLPGCGVDF
260 270 280 290 300
THSRLSNLLG IRKRQPFQEG FRITYDDLEG GNIPALLDVD AYQASLKDDT
310 320 330 340 350
EQGGDGAGGG NNSGSGAEEN SNAAAAAMQP VEDMNDHAIR GDTFATRAEE
360 370 380 390 400
KRAEAEAAAE AAAPAAQPEV EKPQKKPVIK PLTEDSKKRS YNLISNDSTF
410 420 430 440 450
TQYRSWYLAY NYGDPQTGIR SWTLLCTPDV TCGSEQVYWS LPDMMQDPVT
460 470 480 490 500
FRSTSQISNF PVVGAELLPV HSKSFYNDQA VYSQLIRQFT SLTHVFNRFP
510 520 530 540 550
ENQILARPPA PTITTVSENV PALTDHGTLP LRNSIGGVQR VTITDARRRT
560 570
CPYVYKALGI VSPRVLSSRT F
Length:571
Mass (Da):63,255
Last modified:July 21, 1986 - v1
Checksum:iE9807342982B1BC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01917 Genomic DNA. Translation: AAA92211.1.
PIRiA03847. XZAD32.
RefSeqiAP_000170.1. AC_000007.1.
NP_040521.1. NC_001405.1.

Genome annotation databases

GeneIDi2652996.
KEGGivg:2652996.

Cross-referencesi

Web resourcesi

Virus Particle ExploreR db

Icosahedral capsid structure

Virus Particle ExploreR db

Icosahedral capsid structure in complex with an ad2 N-terminal fiber peptide

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01917 Genomic DNA. Translation: AAA92211.1.
PIRiA03847. XZAD32.
RefSeqiAP_000170.1. AC_000007.1.
NP_040521.1. NC_001405.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X9PX-ray3.30A49-571[»]
1X9TX-ray3.50A49-571[»]
2C6SX-ray3.60A/B/C/D/E/F/G/H/I/J/K/L/M/N/O49-571[»]
2C9Felectron microscopy16.50A/B/C/D/E49-571[»]
2C9Gelectron microscopy9.30A/B/C/D/E49-571[»]
4V4Uelectron microscopy10.00A/B/C/D/E49-571[»]
ProteinModelPortaliP03276.
SMRiP03276.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP03276.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2652996.
KEGGivg:2652996.

Miscellaneous databases

EvolutionaryTraceiP03276.

Family and domain databases

InterProiIPR002605. Adeno_Penton_B.
[Graphical view]
PfamiPF01686. Adeno_Penton_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAPSP_ADE02
AccessioniPrimary (citable) accession number: P03276
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.