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Protein

Fiber protein

Gene

L5

Organism
Human adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Forms spikes that protrude from each vertex of the icosahedral capsid. Interacts with host coxsackievirus and adenovirus receptor CXADR located at the cell tight junctions to provide virion initial attachment to target cell. The fiber protein binds to CXADR with a higher affinity than CXADR binds to itself, thereby blocking the cell-cell adhesion function of CXADR dimers and leading to local disruption of the tight junction. Fiber protein present on neo-synthesized particles may thus disrupt the junctional integrity in order to facilitate further neighboring cells infection. Fiber proteins are shed during virus entry, when virus is still at the cell surface. Fiber shedding is dependent on viral CXADR drifting motion and subsequent binding to immobile integrins. Heparan sulfate might also play a role in virus binding.4 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral attachment to host adhesion receptor, Viral attachment to host cell, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Fiber protein
Short name:
SPIKE
Alternative name(s):
Protein IV
Gene namesi
ORF Names:L5
OrganismiHuman adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Taxonomic identifieri10515 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008167 Componenti: Genome

Subcellular locationi

  • Virion 1 Publication
  • Host nucleus 1 Publication

  • Note: Anchored to the pentons, protrudes from the virion surface. Present in 36 copies per virion.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002217861 – 582Fiber proteinAdd BLAST582

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei325Phosphoserine; by host1 Publication1
Modified residuei351Phosphoserine; by host1 Publication1

Post-translational modificationi

O-glycosylated; glycans contain N-acetylglucosamine and may play a role in fiber assembly and stabilization.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

PTM databases

iPTMnetiP03275.
UniCarbKBiP03275.

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homotrimer; arranged in a triple beta-spiral. Interacts with host receptor CXADR. Interacts (via N-terminal tail region) with pentons (Probable).2 Publications

Structurei

Secondary structure

1582
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi323 – 325Combined sources3
Beta strandi327 – 330Combined sources4
Beta strandi333 – 336Combined sources4
Beta strandi342 – 344Combined sources3
Turni349 – 353Combined sources5
Beta strandi357 – 359Combined sources3
Beta strandi365 – 367Combined sources3
Beta strandi373 – 375Combined sources3
Beta strandi381 – 383Combined sources3
Turni385 – 387Combined sources3
Beta strandi389 – 391Combined sources3
Helixi397 – 399Combined sources3
Beta strandi400 – 403Combined sources4
Beta strandi413 – 415Combined sources3
Beta strandi419 – 428Combined sources10
Beta strandi431 – 440Combined sources10
Helixi446 – 448Combined sources3
Beta strandi450 – 461Combined sources12
Beta strandi469 – 473Combined sources5
Beta strandi479 – 482Combined sources4
Helixi495 – 497Combined sources3
Turni501 – 503Combined sources3
Helixi512 – 514Combined sources3
Beta strandi515 – 521Combined sources7
Helixi522 – 524Combined sources3
Beta strandi528 – 537Combined sources10
Helixi538 – 540Combined sources3
Beta strandi550 – 558Combined sources9
Beta strandi574 – 580Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QHVX-ray1.51A388-582[»]
1QIUX-ray2.40A/B/C/D/E/F319-582[»]
1V1HX-ray1.90A/B/C/D/E/F319-392[»]
1V1IX-ray1.90A/B/C319-398[»]
1X9TX-ray3.50B1-20[»]
2C9Felectron microscopy16.50S/T/U/V/W1-19[»]
4AR2X-ray3.80P1-20[»]
4V4Uelectron microscopy10.00S/T/U/V/W10-19[»]
ProteinModelPortaliP03275.
SMRiP03275.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03275.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati45 – 59Shaft 1Add BLAST15
Repeati60 – 75Shaft 2Add BLAST16
Repeati76 – 95Shaft 3Add BLAST20
Repeati96 – 109Shaft 4Add BLAST14
Repeati110 – 124Shaft 5Add BLAST15
Repeati125 – 139Shaft 6Add BLAST15
Repeati140 – 154Shaft 7Add BLAST15
Repeati155 – 170Shaft 8Add BLAST16
Repeati171 – 185Shaft 9Add BLAST15
Repeati186 – 200Shaft 10Add BLAST15
Repeati201 – 217Shaft 11Add BLAST17
Repeati218 – 232Shaft 12Add BLAST15
Repeati233 – 248Shaft 13Add BLAST16
Repeati249 – 263Shaft 14Add BLAST15
Repeati264 – 277Shaft 15Add BLAST14
Repeati278 – 294Shaft 16Add BLAST17
Repeati295 – 314Shaft 17Add BLAST20
Repeati315 – 331Shaft 18Add BLAST17
Repeati332 – 354Shaft 19Add BLAST23
Repeati355 – 370Shaft 20Add BLAST16
Repeati371 – 386Shaft 21Add BLAST16
Repeati387 – 392Shaft 226

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 44Tail (penton base attachment)Add BLAST44
Regioni45 – 392Shaft regionAdd BLAST348
Regioni393 – 398Spacer6
Regioni399 – 582HeadAdd BLAST184

Domaini

The tail region anchors the fiber to penton base capsomers, whereas the shaft, built from several repeated motifs, allows the knob to protrude from the virion.

Sequence similaritiesi

Belongs to the adenoviridae fiber family.Curated
Contains 22 Shaft repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

KOiK19262.

Family and domain databases

Gene3Di2.10.25.20. 3 hits.
2.60.90.10. 1 hit.
InterProiIPR000931. Adeno_fibre.
IPR000978. Adeno_fibre_knob.
IPR000939. Adenobir_fibre_prot_rpt/shaft.
IPR008982. Adenovirus_pIV-rel_att.
IPR009013. Attachment_protein_shaft_dom.
[Graphical view]
PfamiPF00541. Adeno_knob. 1 hit.
PF00608. Adeno_shaft. 9 hits.
[Graphical view]
PRINTSiPR00307. ADENOVSFIBRE.
SUPFAMiSSF49835. SSF49835. 1 hit.
SSF51225. SSF51225. 3 hits.

Sequencei

Sequence statusi: Complete.

P03275-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRARPSEDT FNPVYPYDTE TGPPTVPFLT PPFVSPNGFQ ESPPGVLSLR
60 70 80 90 100
VSEPLDTSHG MLALKMGSGL TLDKAGNLTS QNVTTVTQPL KKTKSNISLD
110 120 130 140 150
TSAPLTITSG ALTVATTAPL IVTSGALSVQ SQAPLTVQDS KLSIATKGPI
160 170 180 190 200
TVSDGKLALQ TSAPLSGSDS DTLTVTASPP LTTATGSLGI NMEDPIYVNN
210 220 230 240 250
GKIGIKISGP LQVAQNSDTL TVVTGPGVTV EQNSLRTKVA GAIGYDSSNN
260 270 280 290 300
MEIKTGGGMR INNNLLILDV DYPFDAQTKL RLKLGQGPLY INASHNLDIN
310 320 330 340 350
YNRGLYLFNA SNNTKKLEVS IKKSSGLNFD NTAIAINAGK GLEFDTNTSE
360 370 380 390 400
SPDINPIKTK IGSGIDYNEN GAMITKLGAG LSFDNSGAIT IGNKNDDKLT
410 420 430 440 450
LWTTPDPSPN CRIHSDNDCK FTLVLTKCGS QVLATVAALA VSGDLSSMTG
460 470 480 490 500
TVASVSIFLR FDQNGVLMEN SSLKKHYWNF RNGNSTNANP YTNAVGFMPN
510 520 530 540 550
LLAYPKTQSQ TAKNNIVSQV YLHGDKTKPM ILTITLNGTS ESTETSEVST
560 570 580
YSMSFTWSWE SGKYTTETFA TNSYTFSYIA QE
Length:582
Mass (Da):61,919
Last modified:July 21, 1986 - v1
Checksum:i8C315CE5EDC9505F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01917 Genomic DNA. Translation: AAA92223.1.
PIRiA93722. ERADF2.
RefSeqiAP_000190.1. AC_000007.1.
NP_040533.1. NC_001405.1.

Genome annotation databases

GeneIDi2652999.
KEGGivg:2652999.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01917 Genomic DNA. Translation: AAA92223.1.
PIRiA93722. ERADF2.
RefSeqiAP_000190.1. AC_000007.1.
NP_040533.1. NC_001405.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QHVX-ray1.51A388-582[»]
1QIUX-ray2.40A/B/C/D/E/F319-582[»]
1V1HX-ray1.90A/B/C/D/E/F319-392[»]
1V1IX-ray1.90A/B/C319-398[»]
1X9TX-ray3.50B1-20[»]
2C9Felectron microscopy16.50S/T/U/V/W1-19[»]
4AR2X-ray3.80P1-20[»]
4V4Uelectron microscopy10.00S/T/U/V/W10-19[»]
ProteinModelPortaliP03275.
SMRiP03275.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP03275.
UniCarbKBiP03275.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2652999.
KEGGivg:2652999.

Phylogenomic databases

KOiK19262.

Miscellaneous databases

EvolutionaryTraceiP03275.

Family and domain databases

Gene3Di2.10.25.20. 3 hits.
2.60.90.10. 1 hit.
InterProiIPR000931. Adeno_fibre.
IPR000978. Adeno_fibre_knob.
IPR000939. Adenobir_fibre_prot_rpt/shaft.
IPR008982. Adenovirus_pIV-rel_att.
IPR009013. Attachment_protein_shaft_dom.
[Graphical view]
PfamiPF00541. Adeno_knob. 1 hit.
PF00608. Adeno_shaft. 9 hits.
[Graphical view]
PRINTSiPR00307. ADENOVSFIBRE.
SUPFAMiSSF49835. SSF49835. 1 hit.
SSF51225. SSF51225. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSPIKE_ADE02
AccessioniPrimary (citable) accession number: P03275
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.