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Protein

Fiber protein

Gene

L5

Organism
Human adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Forms spikes that protrude from each vertex of the icosahedral capsid. Interacts with host coxsackievirus and adenovirus receptor CXADR located at the cell tight junctions to provide virion initial attachment to target cell. The fiber protein binds to CXADR with a higher affinity than CXADR binds to itself, thereby blocking the cell-cell adhesion function of CXADR dimers and leading to local disruption of the tight junction. Fiber protein present on neo-synthesized particles may thus disrupt the junctional integrity in order to facilitate further neighboring cells infection. Fiber proteins are shed during virus entry, when virus is still at the cell surface. Fiber shedding is dependent on viral CXADR drifting motion and subsequent binding to immobile integrins. Heparan sulfate might also play a role in virus binding.4 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Viral attachment to host adhesion receptor, Viral attachment to host cell, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Fiber protein
Short name:
SPIKE
Alternative name(s):
Protein IV
Gene namesi
ORF Names:L5
OrganismiHuman adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Taxonomic identifieri10515 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008167 Componenti: Genome

Subcellular locationi

  • Virion 1 Publication
  • Host nucleus 1 Publication

  • Note: Anchored to the pentons, protrudes from the virion surface. Present in 36 copies per virion.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 582582Fiber proteinPRO_0000221786Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei325 – 3251Phosphoserine; by host1 Publication
Modified residuei351 – 3511Phosphoserine; by host1 Publication

Post-translational modificationi

O-glycosylated; glycans contain N-acetylglucosamine and may play a role in fiber assembly and stabilization.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

PTM databases

iPTMnetiP03275.
UniCarbKBiP03275.

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Homotrimer; arranged in a triple beta-spiral. Interacts with host receptor CXADR. Interacts (via N-terminal tail region) with pentons (Probable).2 Publications

Structurei

Secondary structure

1
582
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi323 – 3253Combined sources
Beta strandi327 – 3304Combined sources
Beta strandi333 – 3364Combined sources
Beta strandi342 – 3443Combined sources
Turni349 – 3535Combined sources
Beta strandi357 – 3593Combined sources
Beta strandi365 – 3673Combined sources
Beta strandi373 – 3753Combined sources
Beta strandi381 – 3833Combined sources
Turni385 – 3873Combined sources
Beta strandi389 – 3913Combined sources
Helixi397 – 3993Combined sources
Beta strandi400 – 4034Combined sources
Beta strandi413 – 4153Combined sources
Beta strandi419 – 42810Combined sources
Beta strandi431 – 44010Combined sources
Helixi446 – 4483Combined sources
Beta strandi450 – 46112Combined sources
Beta strandi469 – 4735Combined sources
Beta strandi479 – 4824Combined sources
Helixi495 – 4973Combined sources
Turni501 – 5033Combined sources
Helixi512 – 5143Combined sources
Beta strandi515 – 5217Combined sources
Helixi522 – 5243Combined sources
Beta strandi528 – 53710Combined sources
Helixi538 – 5403Combined sources
Beta strandi550 – 5589Combined sources
Beta strandi574 – 5807Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QHVX-ray1.51A388-582[»]
1QIUX-ray2.40A/B/C/D/E/F319-582[»]
1V1HX-ray1.90A/B/C/D/E/F319-392[»]
1V1IX-ray1.90A/B/C319-398[»]
1X9TX-ray3.50B1-20[»]
2C9Felectron microscopy16.50S/T/U/V/W1-19[»]
4AR2X-ray3.80P1-20[»]
4V4Uelectron microscopy10.00S/T/U/V/W10-19[»]
ProteinModelPortaliP03275.
SMRiP03275. Positions 319-582.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03275.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati45 – 5915Shaft 1Add
BLAST
Repeati60 – 7516Shaft 2Add
BLAST
Repeati76 – 9520Shaft 3Add
BLAST
Repeati96 – 10914Shaft 4Add
BLAST
Repeati110 – 12415Shaft 5Add
BLAST
Repeati125 – 13915Shaft 6Add
BLAST
Repeati140 – 15415Shaft 7Add
BLAST
Repeati155 – 17016Shaft 8Add
BLAST
Repeati171 – 18515Shaft 9Add
BLAST
Repeati186 – 20015Shaft 10Add
BLAST
Repeati201 – 21717Shaft 11Add
BLAST
Repeati218 – 23215Shaft 12Add
BLAST
Repeati233 – 24816Shaft 13Add
BLAST
Repeati249 – 26315Shaft 14Add
BLAST
Repeati264 – 27714Shaft 15Add
BLAST
Repeati278 – 29417Shaft 16Add
BLAST
Repeati295 – 31420Shaft 17Add
BLAST
Repeati315 – 33117Shaft 18Add
BLAST
Repeati332 – 35423Shaft 19Add
BLAST
Repeati355 – 37016Shaft 20Add
BLAST
Repeati371 – 38616Shaft 21Add
BLAST
Repeati387 – 3926Shaft 22

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 4444Tail (penton base attachment)Add
BLAST
Regioni45 – 392348Shaft regionAdd
BLAST
Regioni393 – 3986Spacer
Regioni399 – 582184HeadAdd
BLAST

Domaini

The tail region anchors the fiber to penton base capsomers, whereas the shaft, built from several repeated motifs, allows the knob to protrude from the virion.

Sequence similaritiesi

Belongs to the adenoviridae fiber family.Curated
Contains 22 Shaft repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

KOiK19262.

Family and domain databases

Gene3Di2.10.25.20. 3 hits.
2.60.90.10. 1 hit.
InterProiIPR000931. Adeno_fibre.
IPR000978. Adeno_fibre_knob.
IPR000939. Adenobir_fibre_prot_rpt/shaft.
IPR008982. Adenovirus_pIV-rel_att.
IPR009013. Attachment_protein_shaft_dom.
[Graphical view]
PfamiPF00541. Adeno_knob. 1 hit.
PF00608. Adeno_shaft. 8 hits.
[Graphical view]
PRINTSiPR00307. ADENOVSFIBRE.
SUPFAMiSSF49835. SSF49835. 1 hit.
SSF51225. SSF51225. 3 hits.

Sequencei

Sequence statusi: Complete.

P03275-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRARPSEDT FNPVYPYDTE TGPPTVPFLT PPFVSPNGFQ ESPPGVLSLR
60 70 80 90 100
VSEPLDTSHG MLALKMGSGL TLDKAGNLTS QNVTTVTQPL KKTKSNISLD
110 120 130 140 150
TSAPLTITSG ALTVATTAPL IVTSGALSVQ SQAPLTVQDS KLSIATKGPI
160 170 180 190 200
TVSDGKLALQ TSAPLSGSDS DTLTVTASPP LTTATGSLGI NMEDPIYVNN
210 220 230 240 250
GKIGIKISGP LQVAQNSDTL TVVTGPGVTV EQNSLRTKVA GAIGYDSSNN
260 270 280 290 300
MEIKTGGGMR INNNLLILDV DYPFDAQTKL RLKLGQGPLY INASHNLDIN
310 320 330 340 350
YNRGLYLFNA SNNTKKLEVS IKKSSGLNFD NTAIAINAGK GLEFDTNTSE
360 370 380 390 400
SPDINPIKTK IGSGIDYNEN GAMITKLGAG LSFDNSGAIT IGNKNDDKLT
410 420 430 440 450
LWTTPDPSPN CRIHSDNDCK FTLVLTKCGS QVLATVAALA VSGDLSSMTG
460 470 480 490 500
TVASVSIFLR FDQNGVLMEN SSLKKHYWNF RNGNSTNANP YTNAVGFMPN
510 520 530 540 550
LLAYPKTQSQ TAKNNIVSQV YLHGDKTKPM ILTITLNGTS ESTETSEVST
560 570 580
YSMSFTWSWE SGKYTTETFA TNSYTFSYIA QE
Length:582
Mass (Da):61,919
Last modified:July 21, 1986 - v1
Checksum:i8C315CE5EDC9505F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01917 Genomic DNA. Translation: AAA92223.1.
PIRiA93722. ERADF2.
RefSeqiAP_000190.1. AC_000007.1.
NP_040533.1. NC_001405.1.

Genome annotation databases

GeneIDi2652999.
KEGGivg:2652999.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01917 Genomic DNA. Translation: AAA92223.1.
PIRiA93722. ERADF2.
RefSeqiAP_000190.1. AC_000007.1.
NP_040533.1. NC_001405.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QHVX-ray1.51A388-582[»]
1QIUX-ray2.40A/B/C/D/E/F319-582[»]
1V1HX-ray1.90A/B/C/D/E/F319-392[»]
1V1IX-ray1.90A/B/C319-398[»]
1X9TX-ray3.50B1-20[»]
2C9Felectron microscopy16.50S/T/U/V/W1-19[»]
4AR2X-ray3.80P1-20[»]
4V4Uelectron microscopy10.00S/T/U/V/W10-19[»]
ProteinModelPortaliP03275.
SMRiP03275. Positions 319-582.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP03275.
UniCarbKBiP03275.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2652999.
KEGGivg:2652999.

Phylogenomic databases

KOiK19262.

Miscellaneous databases

EvolutionaryTraceiP03275.

Family and domain databases

Gene3Di2.10.25.20. 3 hits.
2.60.90.10. 1 hit.
InterProiIPR000931. Adeno_fibre.
IPR000978. Adeno_fibre_knob.
IPR000939. Adenobir_fibre_prot_rpt/shaft.
IPR008982. Adenovirus_pIV-rel_att.
IPR009013. Attachment_protein_shaft_dom.
[Graphical view]
PfamiPF00541. Adeno_knob. 1 hit.
PF00608. Adeno_shaft. 8 hits.
[Graphical view]
PRINTSiPR00307. ADENOVSFIBRE.
SUPFAMiSSF49835. SSF49835. 1 hit.
SSF51225. SSF51225. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiSPIKE_ADE02
AccessioniPrimary (citable) accession number: P03275
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 11, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

All late proteins expressed from the major late promoter are produced by alternative splicing and alternative polyadenylation of the same gene giving rise to non-overlapping ORFs. A leader sequence is present in the N-terminus of all these mRNAs and is recognized by the viral shutoff protein to provide expression although conventional translation via ribosome scanning from the cap has been shut off in the host cell.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.