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P03265

- DNB2_ADE05

UniProt

P03265 - DNB2_ADE05

Protein

Early E2A DNA-binding protein

Gene

DBP

Organism
Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Binds cooperatively single-stranded DNA in a sequence-independent manner. Involved in DNA-replication, regulation of mRNA formation, and host-range specificity. Zinc is required for DNA binding.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri273 – 28614Sequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. DNA replication Source: InterPro
    2. transcription, DNA-templated Source: InterPro
    3. viral process Source: UniProtKB-KW

    Keywords - Biological processi

    Host-virus interaction

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Early E2A DNA-binding protein
    Gene namesi
    Name:DBP
    OrganismiHuman adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
    Taxonomic identifieri28285 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000004992: Genome

    Subcellular locationi

    Host nucleus
    Note: Accumulates in infected cells.

    GO - Cellular componenti

    1. host cell nucleus Source: UniProtKB-SubCell
    2. viral capsid Source: CACAO

    Keywords - Cellular componenti

    Host nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 529529Early E2A DNA-binding proteinPRO_0000221679Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei195 – 1951PhosphotyrosineCurated

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Keywords - Developmental stagei

    Early protein

    Interactioni

    Subunit structurei

    Interacts with human SRCAP.By similarity

    Protein-protein interaction databases

    IntActiP03265. 5 interactions.
    MINTiMINT-216016.

    Structurei

    Secondary structure

    1
    529
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi180 – 19415
    Helixi199 – 2046
    Helixi213 – 22614
    Helixi237 – 25620
    Beta strandi268 – 2725
    Beta strandi275 – 2784
    Beta strandi287 – 2893
    Beta strandi291 – 2977
    Beta strandi331 – 3388
    Helixi340 – 3423
    Beta strandi343 – 3464
    Beta strandi358 – 3603
    Helixi362 – 37918
    Beta strandi389 – 3946
    Helixi397 – 3993
    Beta strandi401 – 4033
    Beta strandi415 – 4195
    Turni421 – 4244
    Helixi428 – 4303
    Helixi434 – 4418
    Beta strandi444 – 4496
    Beta strandi470 – 4723
    Helixi473 – 48816
    Beta strandi491 – 4933
    Helixi506 – 5083
    Beta strandi524 – 5263

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ADUX-ray3.00A/B174-529[»]
    1ADVX-ray3.20A/B174-529[»]
    1ANVX-ray2.70A174-529[»]
    2WAZX-ray2.30X174-529[»]
    2WB0X-ray1.95X174-529[»]
    DisProtiDP00003.
    ProteinModelPortaliP03265.
    SMRiP03265. Positions 179-529.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP03265.

    Family & Domainsi

    Sequence similaritiesi

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri273 – 28614Sequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Family and domain databases

    Gene3Di1.10.269.10. 1 hit.
    3.90.148.10. 2 hits.
    InterProiIPR005376. Adenovirus_DNA-bd_C.
    IPR003176. Adenovirus_DNA-bd_N.
    [Graphical view]
    PfamiPF02236. Viral_DNA_bi. 1 hit.
    PF03728. Viral_DNA_Zn_bi. 2 hits.
    [Graphical view]
    SUPFAMiSSF47724. SSF47724. 1 hit.
    SSF57917. SSF57917. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P03265-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASREEEQRE TTPERGRGAA RRPPTMEDVS SPSPSPPPPR APPKKRMRRR    50
    IESEDEEDSS QDALVPRTPS PRPSTSAADL AIAPKKKKKR PSPKPERPPS 100
    PEVIVDSEEE REDVALQMVG FSNPPVLIKH GKGGKRTVRR LNEDDPVARG 150
    MRTQEEEEEP SEAESEITVM NPLSVPIVSA WEKGMEAARA LMDKYHVDND 200
    LKANFKLLPD QVEALAAVCK TWLNEEHRGL QLTFTSKKTF VTMMGRFLQA 250
    YLQSFAEVTY KHHEPTGCAL WLHRCAEIEG ELKCLHGSIM INKEHVIEMD 300
    VTSENGQRAL KEQSSKAKIV KNRWGRNVVQ ISNTDARCCV HDAACPANQF 350
    SGKSCGMFFS EGAKAQVAFK QIKAFMQALY PNAQTGHGHL LMPLRCECNS 400
    KPGHAPFLGR QLPKLTPFAL SNAEDLDADL ISDKSVLASV HHPALIVFQC 450
    CNPVYRNSRA QGGGPNCDFK ISAPDLLNAL VMVRSLWSEN FTELPRMVVP 500
    EFKWSTKHQY RNVSLPVAHS DARQNPFDF 529
    Length:529
    Mass (Da):59,139
    Last modified:July 21, 1986 - v1
    Checksum:iAC1582B80F370E23
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73260 Genomic DNA. No translation available.
    X02997 Genomic DNA. Translation: CAA26755.1. Sequence problems.
    PIRiA03833. W7AD25.
    RefSeqiAP_000213.1. AC_000008.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73260 Genomic DNA. No translation available.
    X02997 Genomic DNA. Translation: CAA26755.1 . Sequence problems.
    PIRi A03833. W7AD25.
    RefSeqi AP_000213.1. AC_000008.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ADU X-ray 3.00 A/B 174-529 [» ]
    1ADV X-ray 3.20 A/B 174-529 [» ]
    1ANV X-ray 2.70 A 174-529 [» ]
    2WAZ X-ray 2.30 X 174-529 [» ]
    2WB0 X-ray 1.95 X 174-529 [» ]
    DisProti DP00003.
    ProteinModelPortali P03265.
    SMRi P03265. Positions 179-529.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P03265. 5 interactions.
    MINTi MINT-216016.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P03265.

    Family and domain databases

    Gene3Di 1.10.269.10. 1 hit.
    3.90.148.10. 2 hits.
    InterProi IPR005376. Adenovirus_DNA-bd_C.
    IPR003176. Adenovirus_DNA-bd_N.
    [Graphical view ]
    Pfami PF02236. Viral_DNA_bi. 1 hit.
    PF03728. Viral_DNA_Zn_bi. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47724. SSF47724. 1 hit.
    SSF57917. SSF57917. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Structure and organization of the gene coding for the DNA binding protein of adenovirus type 5."
      Kruijer W., van Schaik F.M.A., Sussenbach J.S.
      Nucleic Acids Res. 9:4439-4457(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The sequence of the genome of adenovirus type 5 and its comparison with the genome of adenovirus type 2."
      Chroboczek J., Bieber F., Jacrot B.
      Virology 186:280-285(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Crystal structure of the adenovirus DNA binding protein reveals a hook-on model for cooperative DNA binding."
      Tucker P.A., Tsernoglou D., Tucker A.D., Coenjaerts F.E.J., Leenders H., van der Vliet P.C.
      EMBO J. 13:2994-3002(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 176-529.

    Entry informationi

    Entry nameiDNB2_ADE05
    AccessioniPrimary (citable) accession number: P03265
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3