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P03265 (DNB2_ADE05) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Early E2A DNA-binding protein
Gene names
Name:DBP
OrganismHuman adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5) [Complete proteome]
Taxonomic identifier28285 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length529 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds cooperatively single-stranded DNA in a sequence-independent manner. Involved in DNA-replication, regulation of mRNA formation, and host-range specificity. Zinc is required for DNA binding.

Subunit structure

Interacts with human SRCAP By similarity.

Subcellular location

Host nucleus. Note: Accumulates in infected cells.

Sequence similarities

Belongs to the adenoviridae E2A DNA-binding protein family.

Ontologies

Keywords
   Biological processHost-virus interaction
   Cellular componentHost nucleus
   Developmental stageEarly protein
   DomainZinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: InterPro

transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

viral process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componenthost cell nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

viral capsid

Inferred from direct assay PubMed 23388198. Source: CACAO

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 529529Early E2A DNA-binding protein
PRO_0000221679

Regions

Zinc finger273 – 28614 Potential

Amino acid modifications

Modified residue1951Phosphotyrosine Probable

Secondary structure

................................................... 529
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P03265 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: AC1582B80F370E23

FASTA52959,139
        10         20         30         40         50         60 
MASREEEQRE TTPERGRGAA RRPPTMEDVS SPSPSPPPPR APPKKRMRRR IESEDEEDSS 

        70         80         90        100        110        120 
QDALVPRTPS PRPSTSAADL AIAPKKKKKR PSPKPERPPS PEVIVDSEEE REDVALQMVG 

       130        140        150        160        170        180 
FSNPPVLIKH GKGGKRTVRR LNEDDPVARG MRTQEEEEEP SEAESEITVM NPLSVPIVSA 

       190        200        210        220        230        240 
WEKGMEAARA LMDKYHVDND LKANFKLLPD QVEALAAVCK TWLNEEHRGL QLTFTSKKTF 

       250        260        270        280        290        300 
VTMMGRFLQA YLQSFAEVTY KHHEPTGCAL WLHRCAEIEG ELKCLHGSIM INKEHVIEMD 

       310        320        330        340        350        360 
VTSENGQRAL KEQSSKAKIV KNRWGRNVVQ ISNTDARCCV HDAACPANQF SGKSCGMFFS 

       370        380        390        400        410        420 
EGAKAQVAFK QIKAFMQALY PNAQTGHGHL LMPLRCECNS KPGHAPFLGR QLPKLTPFAL 

       430        440        450        460        470        480 
SNAEDLDADL ISDKSVLASV HHPALIVFQC CNPVYRNSRA QGGGPNCDFK ISAPDLLNAL 

       490        500        510        520 
VMVRSLWSEN FTELPRMVVP EFKWSTKHQY RNVSLPVAHS DARQNPFDF 

« Hide

References

« Hide 'large scale' references
[1]"Structure and organization of the gene coding for the DNA binding protein of adenovirus type 5."
Kruijer W., van Schaik F.M.A., Sussenbach J.S.
Nucleic Acids Res. 9:4439-4457(1981) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The sequence of the genome of adenovirus type 5 and its comparison with the genome of adenovirus type 2."
Chroboczek J., Bieber F., Jacrot B.
Virology 186:280-285(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Crystal structure of the adenovirus DNA binding protein reveals a hook-on model for cooperative DNA binding."
Tucker P.A., Tsernoglou D., Tucker A.D., Coenjaerts F.E.J., Leenders H., van der Vliet P.C.
EMBO J. 13:2994-3002(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 176-529.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M73260 Genomic DNA. No translation available.
X02997 Genomic DNA. Translation: CAA26755.1. Sequence problems.
PIRW7AD25. A03833.
RefSeqAP_000213.1. AC_000008.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1ADUX-ray3.00A/B174-529[»]
1ADVX-ray3.20A/B174-529[»]
1ANVX-ray2.70A174-529[»]
2WAZX-ray2.30X174-529[»]
2WB0X-ray1.95X174-529[»]
DisProtDP00003.
ProteinModelPortalP03265.
SMRP03265. Positions 179-529.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP03265. 5 interactions.
MINTMINT-216016.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.269.10. 1 hit.
3.90.148.10. 2 hits.
InterProIPR005376. Adenovirus_DNA-bd_C.
IPR003176. Adenovirus_DNA-bd_N.
[Graphical view]
PfamPF02236. Viral_DNA_bi. 1 hit.
PF03728. Viral_DNA_Zn_bi. 2 hits.
[Graphical view]
SUPFAMSSF47724. SSF47724. 1 hit.
SSF57917. SSF57917. 2 hits.
ProtoNetSearch...

Other

EvolutionaryTraceP03265.

Entry information

Entry nameDNB2_ADE05
AccessionPrimary (citable) accession number: P03265
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: March 19, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references