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Protein

DNA-binding protein

Gene

DBP

Organism
Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the elongation phase of viral strand displacement replication by unwinding the template in an ATP-independent fashion, employing its capacity to form multimers. Also enhances the rate of initiation. Released from template upon second strand synthesis. Assembles in complex with viral pTP, viral pol, host NFIA and host POU2F1/OCT1 on viral origin of replication. Covers the whole ssDNA genome during synthesis. The complementary strand synthesis induces its relese from DNA template. May inhibit cellular transcription mediated by the interaction between host SRCAP and CBP.UniRule annotation2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi284Zinc 1UniRule annotationCombined sources1
Metal bindingi286Zinc 1UniRule annotationCombined sources1
Metal bindingi339Zinc 1UniRule annotationCombined sources1
Metal bindingi355Zinc 1UniRule annotationCombined sources1
Metal bindingi396Zinc 2UniRule annotationCombined sources1
Metal bindingi398Zinc 2UniRule annotationCombined sources1
Metal bindingi450Zinc 2UniRule annotationCombined sources1
Metal bindingi467Zinc 2UniRule annotationCombined sources1

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • identical protein binding Source: CAFA
  • single-stranded DNA binding Source: CAFA
  • zinc ion binding Source: CAFA

GO - Biological processi

  • DNA duplex unwinding Source: UniProtKB
  • DNA replication Source: UniProtKB-KW
  • positive regulation of DNA replication Source: UniProtKB
  • transcription, DNA-templated Source: InterPro
  • viral DNA genome replication Source: UniProtKB
  • viral DNA strand displacement replication Source: UniProtKB

Keywordsi

Molecular functionDNA-binding
Biological processDNA replication, Host-virus interaction, Viral DNA replication
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding proteinUniRule annotation
Short name:
DBPUniRule annotation
Alternative name(s):
Early 2A proteinUniRule annotation
Early E2A DNA-binding proteinUniRule annotation
Gene namesi
Name:DBPUniRule annotation
OrganismiHuman adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Taxonomic identifieri28285 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000004992 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation

  • Note: Accumulates in infected cells.UniRule annotation

GO - Cellular componenti

  • host cell nucleus Source: UniProtKB
  • viral capsid Source: CACAO

Keywords - Cellular componenti

Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002216791 – 529DNA-binding proteinAdd BLAST529

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei195Phosphotyrosine; by hostUniRule annotation1

Keywords - PTMi

Phosphoprotein

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Homomultimerizes on viral ssDNA bound to pTP. Forms a initiation complex with viral polymerase, pTP and hosts NFIA and POU2F1/OCT1. Interacts with host SRCAP.UniRule annotation

GO - Molecular functioni

  • identical protein binding Source: CAFA

Protein-protein interaction databases

IntActiP03265. 5 interactors.
MINTiMINT-216016.

Structurei

Secondary structure

1529
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi180 – 194Combined sources15
Helixi199 – 204Combined sources6
Helixi213 – 226Combined sources14
Helixi237 – 256Combined sources20
Beta strandi268 – 272Combined sources5
Beta strandi275 – 278Combined sources4
Beta strandi287 – 289Combined sources3
Beta strandi291 – 297Combined sources7
Beta strandi331 – 338Combined sources8
Helixi340 – 342Combined sources3
Beta strandi343 – 346Combined sources4
Beta strandi358 – 360Combined sources3
Helixi362 – 379Combined sources18
Beta strandi389 – 394Combined sources6
Helixi397 – 399Combined sources3
Beta strandi401 – 403Combined sources3
Beta strandi415 – 419Combined sources5
Turni421 – 424Combined sources4
Helixi428 – 430Combined sources3
Helixi434 – 441Combined sources8
Beta strandi444 – 449Combined sources6
Beta strandi470 – 472Combined sources3
Helixi473 – 488Combined sources16
Beta strandi491 – 493Combined sources3
Helixi506 – 508Combined sources3
Beta strandi524 – 526Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ADUX-ray3.00A/B174-529[»]
1ADVX-ray3.20A/B174-529[»]
1ANVX-ray2.70A174-529[»]
2WAZX-ray2.30X174-529[»]
2WB0X-ray1.95X174-529[»]
DisProtiDP00003.
ProteinModelPortaliP03265.
SMRiP03265.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP03265.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni297 – 331Flexible loopUniRule annotationAdd BLAST35
Regioni513 – 529C-terminal arm, DBP bindingUniRule annotationAdd BLAST17

Domaini

The C-terminal arm bridges DBP molecules together, thereby creating a chain.UniRule annotation

Sequence similaritiesi

Belongs to the adenoviridae E2A DNA-binding protein family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG0900009A.

Family and domain databases

Gene3Di1.10.269.10. 1 hit.
3.90.148.10. 2 hits.
HAMAPiMF_04054. ADV_DNB2. 1 hit.
InterProiView protein in InterPro
IPR005376. Adenovirus_DNA-bd_C.
IPR003176. Adenovirus_DNA-bd_N.
PfamiView protein in Pfam
PF02236. Viral_DNA_bi. 1 hit.
PF03728. Viral_DNA_Zn_bi. 2 hits.
SUPFAMiSSF47724. SSF47724. 1 hit.
SSF57917. SSF57917. 2 hits.

Sequencei

Sequence statusi: Complete.

P03265-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASREEEQRE TTPERGRGAA RRPPTMEDVS SPSPSPPPPR APPKKRMRRR
60 70 80 90 100
IESEDEEDSS QDALVPRTPS PRPSTSAADL AIAPKKKKKR PSPKPERPPS
110 120 130 140 150
PEVIVDSEEE REDVALQMVG FSNPPVLIKH GKGGKRTVRR LNEDDPVARG
160 170 180 190 200
MRTQEEEEEP SEAESEITVM NPLSVPIVSA WEKGMEAARA LMDKYHVDND
210 220 230 240 250
LKANFKLLPD QVEALAAVCK TWLNEEHRGL QLTFTSKKTF VTMMGRFLQA
260 270 280 290 300
YLQSFAEVTY KHHEPTGCAL WLHRCAEIEG ELKCLHGSIM INKEHVIEMD
310 320 330 340 350
VTSENGQRAL KEQSSKAKIV KNRWGRNVVQ ISNTDARCCV HDAACPANQF
360 370 380 390 400
SGKSCGMFFS EGAKAQVAFK QIKAFMQALY PNAQTGHGHL LMPLRCECNS
410 420 430 440 450
KPGHAPFLGR QLPKLTPFAL SNAEDLDADL ISDKSVLASV HHPALIVFQC
460 470 480 490 500
CNPVYRNSRA QGGGPNCDFK ISAPDLLNAL VMVRSLWSEN FTELPRMVVP
510 520
EFKWSTKHQY RNVSLPVAHS DARQNPFDF
Length:529
Mass (Da):59,139
Last modified:July 21, 1986 - v1
Checksum:iAC1582B80F370E23
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73260 Genomic DNA. No translation available.
X02997 Genomic DNA. Translation: CAA26755.1. Sequence problems.
PIRiA03833. W7AD25.
RefSeqiAP_000213.1. AC_000008.1.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDNB2_ADE05
AccessioniPrimary (citable) accession number: P03265
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 5, 2017
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families