Reviewed,
UniProtKB/Swiss-Prot P03259 (E1A_ADE12)
Last modified
September 22, 2009.
Version 57.
History...
Clusters with 100%,
90%,
50% identity |
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Names and origin
| Protein names | Recommended name: Early E1A 29.5 kDa protein |
| Organism | Human adenovirus A serotype 12 (HAdV-12) (Human adenovirus 12) |
| Taxonomic identifier | 28282 [NCBI] |
| Taxonomic lineage | Viruses › dsDNA viruses, no RNA stage › Adenoviridae › Mastadenovirus › Human adenovirus A |
| Virus host | Homo sapiens (Human) [TaxID: 9606] |
Protein attributes
| Sequence length | 266 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | E1A protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1 by direct competition for the same binding site on RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. The tumorigenesis-restraining activity of E1A may be related to the disruption of the host CtBP-CtIP complex through the CtBP binding motif By similarity. |
| Subunit structure | Interacts with host UBE2I. Interaction with host RB1 induces the aberrant dissociation of RB1-E2F1 complex thereby disrupting RB1's activity By similarity. Ref.4 |
| Sequence similarities | Belongs to the adenoviridae E1A protein family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Host-virus interaction Transcription Transcription regulation |
| Coding sequence diversity | Alternative splicing |
| Developmental stage | Early protein |
| Disease | Oncogene |
| Domain | Zinc-finger |
| Ligand | DNA-binding Metal-binding Zinc |
| Molecular function | Activator |
| Gene Ontology (GO) | |
| Biological process | regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcriptionInferred from electronic annotation. Source: UniProtKB-KW virus-host interactionInferred from electronic annotation. Source: InterPro |
| Molecular function | DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Alternative products
| This entry describes 4 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform Early E1A 29.5 kDa protein (identifier: P03259-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Early E1A 26 kDa protein (identifier: P03259-2) The sequence of this isoform differs from the canonical sequence as follows: 160-190: Missing. | ||||||
| Isoform Early E1A 22 kDa protein (identifier: P03259-3) The sequence of this isoform differs from the canonical sequence as follows: 191-234: Missing. 259-266: SVKRPRCN → KCAMGGGR | ||||||
| Isoform Early E1A 6 kDa protein (identifier: P03259-4) The sequence of this isoform differs from the canonical sequence as follows: 31-244: Missing. 245-266: IQEEEREQTVPVDLSVKRPRCN → FPIMSLNLIALWMAMSDPHPRN |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 266 | 266 | Early E1A 29.5 kDa protein | PRO_0000221696 | |||||
Regions | |||||||||
| Zinc finger | 159 – 179 | 21 | Potential | ||||||
| Region | 39 – 47 | 9 | Interaction with RB1 in competition with E2F1 By similarity | ||||||
| Region | 80 – 154 | 75 | Interaction with UBE2I | ||||||
| Region | 107 – 111 | 5 | Interaction with RB1 Potential | ||||||
| Region | 255 – 259 | 5 | CTBP-binding By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 31 – 244 | 214 | Missing in isoform Early E1A 6 kDa protein. | VSP_000200 | |||||
| Alternative sequence | 160 – 190 | 31 | Missing in isoform Early E1A 26 kDa protein. | VSP_000201 | |||||
| Alternative sequence | 191 – 234 | 44 | Missing in isoform Early E1A 22 kDa protein. | VSP_000202 | |||||
| Alternative sequence | 245 – 266 | 22 | IQEEE…RPRCN → FPIMSLNLIALWMAMSDPHP RN in isoform Early E1A 6 kDa protein. | VSP_000203 | |||||
| Alternative sequence | 259 – 266 | 8 | SVKRPRCN → KCAMGGGR in isoform Early E1A 22 kDa protein. | VSP_000204 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Structure and gene organization in the transformed Hind III-G fragment of Ad12." Sugisaki H., Sugimoto K., Takanami M., Shiroki K., Saito I., Shimojo H., Sawada Y., Uemizu Y., Uesugi S., Fujinaga K. Cell 20:777-786(1980) [PubMed: 6251973] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [2] | "Structure of two adenovirus type 12 transforming polypeptides and their evolutionary implications." Perricaudet M., le Moullec J.-M., Tiollais P., Pettersson U. Nature 288:174-176(1980) [PubMed: 7432516] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Nucleotide sequence of human adenovirus type 12 DNA: comparative functional analysis." Sprengel J., Schmitz B., Heuss-Neitzel D., Zock C., Doerfler W. J. Virol. 68:379-389(1994) [PubMed: 8254750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [4] | "mUBC9, a novel adenovirus E1A-interacting protein that complements a yeast cell cycle defect." Hateboer G., Hijmans E.M., Nooij J.B.D., Schlenker S., Jentsch S., Bernards R. J. Biol. Chem. 271:25906-25911(1996) [PubMed: 8824223] [Abstract] Cited for: INTERACTION WITH UBE2I. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | V00004 Genomic DNA. Translation: CAA23400.1. V00004 Genomic DNA. Translation: CAA23401.1. V00004 Genomic DNA. Translation: CAA23402.1. X73487 Genomic DNA. Translation: CAA51877.1. |
| PIR | AQADG2. A03828. |
| RefSeq | NP_040910.2. |
3D structure databases | |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 1460853. |
Family and domain databases | |
| InterPro | IPR014410. Aden_E1A. IPR003853. Adeno_E1A. [Graphical view] |
| Pfam | PF02703. Adeno_E1A. 1 hit. [Graphical view] |
| PIRSF | PIRSF003669. Aden_E1A. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | E1A_ADE12 | ||||||||
| Accession | Primary (citable) accession number: P03259 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Virus (Virus annotation project) | ||||||||
