Reviewed,
UniProtKB/Swiss-Prot P03254 (E1A_ADE02)
Last modified
December 15, 2009.
Version 64.
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Early E1A 32 kDa protein |
| Organism | Human adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2) |
| Taxonomic identifier | 10515 [NCBI] |
| Taxonomic lineage | Viruses › dsDNA viruses, no RNA stage › Adenoviridae › Mastadenovirus |
| Virus host | Homo sapiens (Human) [TaxID: 9606] |
Protein attributes
| Sequence length | 289 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Trans-activates early viral promoters and some cellular promoters. The tumorigenesis-restraining activity of E1A may be related to the disruption of the human CtBP-CtIP complex through the CtBP binding motif. |
| Subunit structure | Interacts with human UBE2I By similarity. |
| Sequence similarities | Belongs to the adenoviridae E1A protein family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Host-virus interaction Transcription Transcription regulation |
| Coding sequence diversity | Alternative splicing |
| Developmental stage | Early protein |
| Domain | Zinc-finger |
| Ligand | DNA-binding Metal-binding Zinc |
| Molecular function | Activator |
| PTM | Phosphoprotein |
| Gene Ontology (GO) | |
| Biological process | regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro transcriptionInferred from electronic annotation. Source: UniProtKB-KW virus-host interactionInferred from electronic annotation. Source: InterPro |
| Molecular function | DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Alternative products
| This entry describes 3 isoforms produced by alternative splicing. [Align] [Select] Note: Isoforms are derived from the E1 region of the genome. | ||||||
| Isoform early E1A 32 kDa protein (identifier: P03254-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform early E1A 26 kDa protein (identifier: P03254-2) The sequence of this isoform differs from the canonical sequence as follows: 140-185: Missing. | ||||||
| Isoform early E1A 6 kDa protein (identifier: P03254-3) The sequence of this isoform differs from the canonical sequence as follows: 29-55: ADNLPPPSHFEPPTLHELYDLDVTAPE → CLNLSLSPSQNRSLQDLPGVLNWCLLS 56-289: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 289 | 289 | Early E1A 32 kDa protein | PRO_0000221692 | |||||
Regions | |||||||||
| Zinc finger | 154 – 174 | 21 | C4-type | ||||||
| Region | 76 – 140 | 65 | Interaction with human UBE2I By similarity | ||||||
| Region | 279 – 283 | 5 | CTBP-binding | ||||||
Amino acid modifications | |||||||||
| Modified residue | 89 | 1 | Phosphoserine; by host By similarity | ||||||
| Modified residue | 219 | 1 | Phosphoserine; by host By similarity | ||||||
| Modified residue | 231 | 1 | Phosphoserine; by host By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 29 – 55 | 27 | ADNLP…VTAPE → CLNLSLSPSQNRSLQDLPGV LNWCLLS in isoform early E1A 6 kDa protein. | VSP_028916 | |||||
| Alternative sequence | 56 – 289 | 234 | Missing in isoform early E1A 6 kDa protein. | VSP_028917 | |||||
| Alternative sequence | 140 – 185 | 46 | Missing in isoform early E1A 26 kDa protein. | VSP_000197 | |||||
Experimental info | |||||||||
| Sequence conflict | 68 | 1 | D → E in AAA92197. Ref.2 | ||||||
| Sequence conflict | 68 | 1 | D → E in AAA92199. Ref.2 | ||||||
| Sequence conflict | 81 | 1 | L → F in AAA92197. Ref.2 | ||||||
| Sequence conflict | 81 | 1 | L → F in AAA92199. Ref.2 | ||||||
Sequences
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References
| [1] | "Structure of two spliced mRNAs from the transforming region of human subgroup C adenoviruses." Perricaudet M., Akusjaervi G., Virtanen A., Pettersson U. Nature 281:694-696(1979) [PubMed: 551290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS EARLY E1A 32 KDA PROTEIN AND EARLY E1A 26 KDA PROTEIN). |
| [2] | "Nucleotide sequences from the adenovirus-2 genome." Gingeras T.R., Sciaky D., Gelinas R.E., Bing-Dong J., Yen C.E., Kelly M.M., Bullock P.A., Parsons B.L., O'Neill K.E., Roberts R.J. J. Biol. Chem. 257:13475-13491(1982) [PubMed: 7142161] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS EARLY E1A 32 KDA PROTEIN; EARLY E1A 26 KDA PROTEIN AND EARLY E1A 6 KDA PROTEIN). |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | J01917 Genomic DNA. Translation: AAA92197.1. J01917 Genomic DNA. Translation: AAA92198.1. J01917 Genomic DNA. Translation: AAA92199.1. |
| PIR | Q2AD2. A03824. |
| RefSeq | AP_000161.1. |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-570N. |
Family and domain databases | |
| InterPro | IPR014410. Aden_E1A. IPR003853. Adeno_E1A. [Graphical view] |
| Pfam | PF02703. Adeno_E1A. 1 hit. [Graphical view] |
| PIRSF | PIRSF003669. Aden_E1A. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | E1A_ADE02 | ||||||||
| Accession | Primary (citable) accession number: P03254 Secondary accession number(s): P24934, Q67788 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Virus (Virus annotation project) | ||||||||
