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Reviewed, UniProtKB/Swiss-Prot P03247 (E1BS_ADE02)

Last modified January 19, 2010. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    E1B protein, small T-antigen
Alternative name(s):
    E1B 19 kDa protein
      Short name=E1B-19K
    E1B-175R
Gene names
Name: E1B
OrganismHuman adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Taxonomic identifier10515 [NCBI]
Taxonomic lineageViruses › dsDNA viruses, no RNA stage › Adenoviridae › Mastadenovirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

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Protein attributes

Sequence length175 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Putative adenovirus Bcl-2 homolog that inhibits apoptosis induced by TNF or FAS pathways, as well as p53-mediated apoptosis. Without E1B 19K function, virus production is compromised because of premature death of host cell. Interacts with Bax protein in cell lysates By similarity.

Subcellular location

Host cell membrane By similarity. Host nucleus envelope By similarity. Host nucleus lamina By similarity. Note: Associated with the plasma and nuclear membranes, and with the insoluble nuclear lamina By similarity.

Sequence similarities

Belongs to the adenoviridae E1B 19 kDa protein family.

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Ontologies

Keywords
   Biological processApoptosis
   Cellular componentHost cell membrane
Host membrane
Host nucleus
Membrane
   Coding sequence diversityAlternative splicing
   Developmental stageEarly protein
Gene Ontology (GO)
   Biological processanti-apoptosis

Traceable author statement. Source: UniProtKB

apoptosis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componenthost cell nuclear lamina

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionlamin binding

Inferred from physical interaction. Source: UniProtKB

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

BNIP3LO602383EBI-849856,EBI-849893From a different organism.

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Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Note: At least five different polypeptides are generated by alternative splicing of a common mRNA precursor.
Isoform E1B-175R (identifier: P03247-1)

Also known as: E1B-19K;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform E1B-495R (identifier: P03244-1)

Also known as: E1B-55K;

The sequence of this isoform can be found in the external entry P03244-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform E1B-155R (identifier: P03244-2)

Also known as: E1B-18K;

The sequence of this isoform can be found in the external entry P03244-2.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform E1B-92R (identifier: P03244-3)

Also known as: E1B-16K;

The sequence of this isoform can be found in the external entry P03244-3.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Isoform E1B-82R (identifier: P03244-4)

Also known as: E1B-15K;

The sequence of this isoform can be found in the external entry P03244-4.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 175175E1B protein, small T-antigen
PRO_0000221710

Regions

Compositional bias89 – 946Poly-Ala
Compositional bias133 – 1364Poly-Leu
Compositional bias155 – 1595Poly-Arg

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Sequences

Sequence LengthMass (Da)Tools
Isoform E1B-175R (E1B-19K) [UniParc].

Last modified March 1, 1992. Version 2.
Checksum: 2FF4279D0CC700B6

FASTA17520,514
        10         20         30         40         50         60 
MEAWECLEDF SAVRNLLEQS SNSTSWFWRF LWGSSQAKLV CRIKEDYKWE FEELLKSCGE 

        70         80         90        100        110        120 
LFDSLNLGHQ ALFQEKVIKT LDFSTPGRAA AAVAFLSFIK DKWSEETHLS GGYLLDFLAM 

       130        140        150        160        170 
HLWRAVVRHK NRLLLLSSVR PAIIPTEEQQ QEEARRRRRQ EQSPWNPRAG LDPRE

« Hide

Isoform E1B-495R (E1B-55K).

See P03244.

FASTA––
Isoform E1B-155R (E1B-18K).

See P03244.

FASTA––
Isoform E1B-92R (E1B-16K).

See P03244.

FASTA––
Isoform E1B-82R (E1B-15K).

See P03244.

FASTA––

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References

[1]"Nucleotide sequences from the adenovirus-2 genome."
Gingeras T.R., Sciaky D., Gelinas R.E., Bing-Dong J., Yen C.E., Kelly M.M., Bullock P.A., Parsons B.L., O'Neill K.E., Roberts R.J.
J. Biol. Chem. 257:13475-13491(1982) [PubMed: 7142161] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Early region 1B of adenovirus 2 encodes two coterminal proteins of 495 and 155 amino acid residues."
Anderson C.W., Schmitt R.C., Smart J.E., Lewis J.B.
J. Virol. 50:387-396(1984) [PubMed: 6323739] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORMS E1B-495R AND E1B-155R).
[3]"Organization of early region 1B of human adenovirus type 2: identification of four differentially spliced mRNAs."
Virtanen A., Pettersson U.
J. Virol. 54:383-391(1985) [PubMed: 3989911] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORMS E1B-495R; E1B-175R; E1B-155R; E1B-92R AND E1B-82R).
[4]"Identification of adenovirus type 2 early region 1B proteins that share the same amino terminus as do the 495R and 155R proteins."
Lewis J.B., Anderson C.W.
J. Virol. 61:3879-3888(1987) [PubMed: 2960832] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORMS E1B-495R; E1B-175R; E1B-155R; E1B-92R AND E1B-82R).

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		J01917 Genomic DNA. Translation: AAA92200.1.
PIRQ1AD22. B03814.
RefSeqAP_000162.1.
NP_040510.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActP03247. 6 interactions.

Genome annotation databases

GeneID2652981.

Family and domain databases

InterProIPR002924. Adenovir_t-Ag_E1B_19kDa.
IPR002475. BCL2_apoptsis.
[Graphical view]
PfamPF01691. Adeno_E1B_19K. 1 hit.
[Graphical view]
PROSITEPS50062. BCL2_FAMILY. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameE1BS_ADE02
AccessionPrimary (citable) accession number: P03247
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 1, 1992
Last modified: January 19, 2010
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectVirus (Virus annotation project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents