P03246 (E1BS_ADE05) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: E1B protein, small T-antigen Alternative name(s): E1B 19 kDa protein Short name=E1B-19K
Organism	Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Taxonomic identifier	28285 [NCBI]
Taxonomic lineage	Viruses › dsDNA viruses, no RNA stage › Adenoviridae › Mastadenovirus
Virus host	Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative adenovirus Bcl-2 homolog that inhibits apoptosis induced by TNF or FAS pathways, as well as p53-mediated apoptosis. Without E1B 19K function, virus production is compromised because of premature death of host cell. Interacts with Bax protein in cell lysates.
Subcellular location	Host cell membrane. Host nucleus envelope. Host nucleus lamina. Note: Associated with the plasma and nuclear membranes, and with the insoluble nuclear lamina.
Sequence similarities	Belongs to the adenoviridae E1B 19 kDa protein family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Host cell membrane Host membrane Host nucleus Membrane
Coding sequence diversity	Alternative splicing
Developmental stage	Early protein
Gene Ontology (GO)
Biological process	anti-apoptosis Inferred from electronic annotation. Source: InterPro apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	host cell nuclear lamina Inferred from electronic annotation. Source: UniProtKB-SubCell host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	lamin binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Note: At least five different polypeptides are generated by alternative splicing of a common mRNA precursor.

Isoform E1B-176R (identifier: P03246-1)

Also known as: E1B-19K;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform E1B-496R (identifier: P03243-1)

Also known as: E1B-55K;

The sequence of this isoform can be found in the external entry P03243.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform E1B-156R (identifier: P03243-2)

Also known as: E1B-18K;

The sequence of this isoform can be found in the external entry P03243.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform E1B-93R (identifier: P03243-3)

Also known as: E1B-16K;

The sequence of this isoform can be found in the external entry P03243.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform E1B-84R (identifier: P03243-4)

Also known as: E1B-15K;

The sequence of this isoform can be found in the external entry P03243.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 176	176	E1B protein, small T-antigen		PRO_0000221712
Regions
	Compositional bias	89 – 94	6	Poly-Ala
	Compositional bias	133 – 136	4	Poly-Leu
	Compositional bias	149 – 153	5	Poly-Gln
	Compositional bias	157 – 160	4	Poly-Arg
Experimental info
	Mutagenesis	51	1	F → S: No interaction with BAX and loss of anti-apoptotic activity.
	Mutagenesis	87	1	G → A: No interaction with BAX and loss of anti-apoptotic activity.

Sequences

	Sequence		Length	Mass (Da)
	Isoform E1B-176R (E1B-19K) [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 5ECBDA61978C29CB	FASTA	176	20,614
	10 20 30 40 50 60 MEAWECLEDF SAVRNLLEQS SNSTSWFWRF LWGSSQAKLV CRIKEDYKWE FEELLKSCGE 70 80 90 100 110 120 LFDSLNLGHQ ALFQEKVIKT LDFSTPGRAA AAVAFLSFIK DKWSEETHLS GGYLLDFLAM 130 140 150 160 170 HLWRAVVRHK NRLLLLSSVR PAIIPTEEQQ QQQEEARRRR QEQSPWNPRA GLDPRE
	Isoform E1B-496R (E1B-55K) [UniParc]. See P03243.		–	–
	Isoform E1B-156R (E1B-18K) [UniParc]. See P03243.		–	–
	Isoform E1B-93R (E1B-16K) [UniParc]. See P03243.		–	–
	Isoform E1B-84R (E1B-15K) [UniParc]. See P03243.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 2.2 kb E1b mRNA of human Ad12 and Ad5 codes for two tumor antigens starting at different AUG triplets." Bos J.L., Polder L.J., Bernards R., Schrier P.I., van den Elsen P.J., van der Eb A.J., van Ormondt H. Cell 27:121-131(1981) [PubMed: 7326748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of the transforming early region E1 of adenovirus type 5 DNA." van Ormondt H., Maat J., van Beveren C.P. Gene 11:299-309(1980) [PubMed: 6260576] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The sequence of the genome of adenovirus type 5 and its comparison with the genome of adenovirus type 2." Chroboczek J., Bieber F., Jacrot B. Virology 186:280-285(1992) [PubMed: 1727603] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The E1B 19K protein blocks apoptosis by interacting with and inhibiting the p53-inducible and death-promoting Bax protein." Han J., Sabbatini P., Perez D., Rao L., Modha D., White E. Genes Dev. 10:461-477(1996) [PubMed: 8600029] [Abstract] Cited for: MUTAGENESIS, INTERACTION WITH BAX.
[5]	"Structure of two spliced mRNAs from the transforming region of human subgroup C adenoviruses." Perricaudet M., Akusjaervi G., Virtanen A., Pettersson U. Nature 281:694-696(1979) [PubMed: 551290] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS E1B-496R; E1B-176R; E1B-156R; E1B-93R AND E1B-84R).
[6]	"Characterization of the 55K adenovirus type 5 E1B product and related proteins." Takayesu D., Teodoro J.G., Whalen S.G., Branton P.E. J. Gen. Virol. 75:789-798(1994) [PubMed: 8151295] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS E1B-496R; E1B-176R; E1B-156R; E1B-93R AND E1B-84R).

Cross-references

Sequence databases
EMBL GenBank DDBJ	M73260 Genomic DNA. No translation available. X02996 Genomic DNA. Translation: CAA26741.1.
PIR	Q1AD25. A03814.
RefSeq	AP_000198.1. AC_000008.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002924. Adenovir_t-Ag_E1B_19kDa. IPR002475. Bcl2-like_apoptosis. [Graphical view]
Pfam	PF01691. Adeno_E1B_19K. 1 hit. [Graphical view]
PROSITE	PS50062. BCL2_FAMILY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E1BS_ADE05

Accession

Primary (citable) accession number: P03246

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	May 3, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program