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Reviewed, UniProtKB/Swiss-Prot P03244 (E1BL_ADE02)

Last modified November 24, 2009. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    E1B protein, large T-antigen
Alternative name(s):
    E1B 55 kDa protein
      Short name=E1B-55K
    E1B-495R
Gene names
Name: E1B
OrganismHuman adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Taxonomic identifier10515 [NCBI]
Taxonomic lineageViruses › dsDNA viruses, no RNA stage › Adenoviridae › Mastadenovirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

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Protein attributes

Sequence length495 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Sequence similarities

Belongs to the adenoviridae E1B 55 kDa protein family.

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Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   Developmental stageEarly protein
Gene Ontology (GO)
   Biological processresponse to external stimulus

Inferred from electronic annotation. Source: InterPro

   Molecular functionprotein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

DAXXQ9UER73EBI-1561155,EBI-77321From a different organism.

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Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Note: At least five different polypeptides are generated by alternative splicing of a common mRNA precursor.
Isoform E1B-495R (identifier: P03244-1)

Also known as: E1B-55K;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform E1B-155R (identifier: P03244-2)

Also known as: E1B-18K;

The sequence of this isoform differs from the canonical sequence as follows:
     79-418: Missing.
Isoform E1B-92R (identifier: P03244-3)

Also known as: E1B-16K;

The sequence of this isoform differs from the canonical sequence as follows:
     79-92: VAELFPELRRILTI → EGGVPTLPMQFESH
     93-495: Missing.
Isoform E1B-82R (identifier: P03244-4)

Also known as: E1B-15K;

The sequence of this isoform differs from the canonical sequence as follows:
     79-82: VAEL → QPPP
     83-495: Missing.
Isoform E1B-175R (identifier: P03247-1)

Also known as: E1B-19K;

The sequence of this isoform can be found in the external entry P03247-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 495495E1B protein, large T-antigen
PRO_0000221724

Regions

Compositional bias54 – 607Poly-Ala

Natural variations

Alternative sequence79 – 418340Missing in isoform E1B-155R.
VSP_033955
Alternative sequence79 – 9214VAELF…RILTI → EGGVPTLPMQFESH in isoform E1B-92R.
VSP_033956
Alternative sequence79 – 824VAEL → QPPP in isoform E1B-82R.
VSP_033957
Alternative sequence83 – 495413Missing in isoform E1B-82R.
VSP_033958
Alternative sequence93 – 495403Missing in isoform E1B-92R.
VSP_033959

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Sequences

Sequence LengthMass (Da)Tools
Isoform E1B-495R (E1B-55K) [UniParc].

Last modified March 1, 1992. Version 2.
Checksum: D6665B27183720CC

FASTA49554,909
        10         20         30         40         50         60 
MERRNPSERG VPAGFSGHAS VESGGETQES PATVVFRPPG NNTDGGATAG GSQAAAAAGA 

        70         80         90        100        110        120 
EPMEPESRPG PSGMNVVQVA ELFPELRRIL TINEDGQGLK GVKRERGASE ATEEARNLTF 

       130        140        150        160        170        180 
SLMTRHRPEC VTFQQIKDNC ANELDLLAQK YSIEQLTTYW LQPGDDFEEA IRVYAKVALR 

       190        200        210        220        230        240 
PDCKYKISKL VNIRNCCYIS GNGAEVEIDT EDRVAFRCSM INMWPGVLGM DGVVIMNVRF 

       250        260        270        280        290        300 
TGPNFSGTVF LANTNLILHG VSFYGFNNTC VEAWTDVRVR GCAFYCCWKG VVCRPKSRAS 

       310        320        330        340        350        360 
IKKCLFERCT LGILSEGNSR VRHNVASDCG CFMLVKSVAV IKHNMVCGNC EDRASQMLTC 

       370        380        390        400        410        420 
SDGNCHLLKT IHVASHSRKA WPVFEHNILT RCSLHLGNRR GVFLPYQCNL SHTKILLEPE 

       430        440        450        460        470        480 
SMSKVNLNGV FDMTMKIWKV LRYDETRTRC RPCECGGKHI RNQPVMLDVT EELRPDHLVL 

       490 
ACTRAEFGSS DEDTD

« Hide

Isoform E1B-155R (E1B-18K).

Checksum: 2D703C8E9211DB08
Show »

FASTA15516,561
Isoform E1B-92R (E1B-16K).

Checksum: AA7F6DB83B4C9273
Show »

FASTA929,217
Isoform E1B-82R (E1B-15K).

Checksum: 94A21B6DDAB11754
Show »

FASTA828,126
Isoform E1B-175R (E1B-19K).

See P03247.

FASTA––

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References

[1]"Nucleotide sequences from the adenovirus-2 genome."
Gingeras T.R., Sciaky D., Gelinas R.E., Bing-Dong J., Yen C.E., Kelly M.M., Bullock P.A., Parsons B.L., O'Neill K.E., Roberts R.J.
J. Biol. Chem. 257:13475-13491(1982) [PubMed: 7142161] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Early region 1B of adenovirus 2 encodes two coterminal proteins of 495 and 155 amino acid residues."
Anderson C.W., Schmitt R.C., Smart J.E., Lewis J.B.
J. Virol. 50:387-396(1984) [PubMed: 6323739] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORMS E1B-495R AND E1B-155R).
[3]"Organization of early region 1B of human adenovirus type 2: identification of four differentially spliced mRNAs."
Virtanen A., Pettersson U.
J. Virol. 54:383-391(1985) [PubMed: 3989911] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORMS E1B-495R; E1B-175R; E1B-155R; E1B-92R AND E1B-82R).
[4]"Identification of adenovirus type 2 early region 1B proteins that share the same amino terminus as do the 495R and 155R proteins."
Lewis J.B., Anderson C.W.
J. Virol. 61:3879-3888(1987) [PubMed: 2960832] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORMS E1B-495R; E1B-175R; E1B-155R; E1B-92R AND E1B-82R).

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		J01917 Genomic DNA. Translation: AAA92201.1.
J01917 Genomic DNA. Translation: AAA92202.1.
J01917 Genomic DNA. Translation: AAA92203.1.
PIRQ1AD52. B03809.
RefSeqAP_000163.1.
NP_040511.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActP03244. 1 interaction.

Genome annotation databases

GeneID2652981.

Family and domain databases

InterProIPR006717. Adeno_E1B_55K_N.
IPR002612. AdenoE1B_55kDa.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamPF01696. Adeno_E1B_55K. 1 hit.
PF04623. Adeno_E1B_55K_N. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameE1BL_ADE02
AccessionPrimary (citable) accession number: P03244
Secondary accession number(s): Q67789, Q67790
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 1, 1992
Last modified: November 24, 2009
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectVirus (Virus annotation project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents