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Protein

E1B 55 kDa protein

Gene

E1B

Organism
Human adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a major role to prevent cellular inhibition of viral genome replication. Assembles an SCF-like E3 ubiquitin ligase complex based on the cellular proteins TCEB2, TCEB1, CUL5 and RBX1, in cooperation with viral E4orf6. This viral RING-type ligase ubiquitinates cellular substrates and targets them to proteasomal degradation: TP53/p53, LIG4, MRE11-RAD50-NBS1 (MRN) complex, ITGA3, DAXX and BLM. Degradation of host TP53/p53 activity is essential for preventing E1A-induced TP53 accumulation that would otherwise lead to cell apoptosis and growth arrest. E1B-55K also inactivates TP53 transcription-factor activity by binding its transactivation domain. E1B-55K also functions as a SUMO1 E3 ligase for TP53 which causes the latter to be sequestered in promyelocytic leukemia (PML) nuclear bodies thereby contributing to maximal inhibition of TP53 function.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Inhibition of host PKR by virus, Modulation of host cell apoptosis by virus, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
E1B 55 kDa protein
Short name:
E1B-55K
Alternative name(s):
E1B protein, large T-antigen
E1B-495R
Gene namesi
Name:E1B
OrganismiHuman adenovirus C serotype 2 (HAdV-2) (Human adenovirus 2)
Taxonomic identifieri10515 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageAdenoviridaeMastadenovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008167 Componenti: Genome

Subcellular locationi

  • Host nucleus 1 Publication
  • Host cytoplasm 1 Publication

  • Note: Colocalizes with host TP53 to host PML nuclear bodies. PML localization of E1B-55K is necessary for E1B-55K-dependent SUMOylation of TP53.By similarity

GO - Cellular componenti

  • host cell cytoplasm Source: UniProtKB-SubCell
  • host cell nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002217241 – 495E1B 55 kDa proteinAdd BLAST495

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Interacts with the transactivation domain of TP53 (via N-terminus); this interaction leads to the inhibition of TP53 function and/or its degradation (By similarity). Interacts with host PML-4 and PML-5; this interaction promotes efficient subnuclear targeting of E1B-55K to PML nuclear bodies (By similarity). Interacts with E4-ORF3 protein (PubMed:10211970). Interacts with E4-ORF6 protein (PubMed:11070042). Interacts with host DAXX protein; this interaction might alterate the normal interactions of DAXX, PML, and p53, which may contribute to cell transformation (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DAXXQ9UER74EBI-1561155,EBI-77321From a different organism.

Protein-protein interaction databases

IntActiP03244. 3 interactors.
MINTiMINT-126636.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi54 – 60Poly-Ala7

Domaini

Contains a PML interaction motif that allows the subnuclear PML localization.By similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR006717. Adeno_E1B_55K_N.
IPR002612. Adeno_E1B_55kDa.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF01696. Adeno_E1B_55K. 1 hit.
PF04623. Adeno_E1B_55K_N. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform E1B-495R (identifier: P03244-1) [UniParc]FASTAAdd to basket
Also known as: E1B-55K

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERRNPSERG VPAGFSGHAS VESGGETQES PATVVFRPPG NNTDGGATAG
60 70 80 90 100
GSQAAAAAGA EPMEPESRPG PSGMNVVQVA ELFPELRRIL TINEDGQGLK
110 120 130 140 150
GVKRERGASE ATEEARNLTF SLMTRHRPEC VTFQQIKDNC ANELDLLAQK
160 170 180 190 200
YSIEQLTTYW LQPGDDFEEA IRVYAKVALR PDCKYKISKL VNIRNCCYIS
210 220 230 240 250
GNGAEVEIDT EDRVAFRCSM INMWPGVLGM DGVVIMNVRF TGPNFSGTVF
260 270 280 290 300
LANTNLILHG VSFYGFNNTC VEAWTDVRVR GCAFYCCWKG VVCRPKSRAS
310 320 330 340 350
IKKCLFERCT LGILSEGNSR VRHNVASDCG CFMLVKSVAV IKHNMVCGNC
360 370 380 390 400
EDRASQMLTC SDGNCHLLKT IHVASHSRKA WPVFEHNILT RCSLHLGNRR
410 420 430 440 450
GVFLPYQCNL SHTKILLEPE SMSKVNLNGV FDMTMKIWKV LRYDETRTRC
460 470 480 490
RPCECGGKHI RNQPVMLDVT EELRPDHLVL ACTRAEFGSS DEDTD
Length:495
Mass (Da):54,909
Last modified:March 1, 1992 - v2
Checksum:iD6665B27183720CC
GO
Isoform E1B-155R (identifier: P03244-2)
Also known as: E1B-18K
Sequence is not available
Length:
Mass (Da):
Isoform E1B-92R (identifier: P03244-3)
Also known as: E1B-16K
Sequence is not available
Length:
Mass (Da):
Isoform E1B-82R (identifier: P03244-4)
Also known as: E1B-15K
Sequence is not available
Length:
Mass (Da):
Isoform E1B-175R (identifier: P03247-1) [UniParc]FASTAAdd to basket
Also known as: E1B-19K
The sequence of this isoform can be found in the external entry P03247.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:175
Mass (Da):20,514
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01917 Genomic DNA. Translation: AAA92201.1.
J01917 Genomic DNA. Translation: AAA92202.1.
J01917 Genomic DNA. Translation: AAA92203.1.
PIRiB03809. Q1AD52.
RefSeqiAP_000163.1. AC_000007.1. [P03244-1]
NP_040511.1. NC_001405.1.

Genome annotation databases

GeneIDi2652981.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01917 Genomic DNA. Translation: AAA92201.1.
J01917 Genomic DNA. Translation: AAA92202.1.
J01917 Genomic DNA. Translation: AAA92203.1.
PIRiB03809. Q1AD52.
RefSeqiAP_000163.1. AC_000007.1. [P03244-1]
NP_040511.1. NC_001405.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP03244. 3 interactors.
MINTiMINT-126636.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2652981.

Family and domain databases

InterProiIPR006717. Adeno_E1B_55K_N.
IPR002612. Adeno_E1B_55kDa.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF01696. Adeno_E1B_55K. 1 hit.
PF04623. Adeno_E1B_55K_N. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiE1B55_ADE02
AccessioniPrimary (citable) accession number: P03244
Secondary accession number(s): Q67789, Q67790
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: March 1, 1992
Last modified: September 7, 2016
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.