Reviewed, UniProtKB/Swiss-Prot P03243 (E1BL_ADE05)

Names and origin

Protein names	Recommended name:     E1B protein, large T-antigen Alternative name(s):     E1B 55 kDa protein       Short name=E1B-55K
Organism	Human adenovirus C serotype 5 (HAdV-5) (Human adenovirus 5)
Taxonomic identifier	28285 [NCBI]
Taxonomic lineage	Viruses › dsDNA viruses, no RNA stage › Adenoviridae › Mastadenovirus
Virus host	Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Sequence similarities

Belongs to the adenoviridae E1B 55 kDa protein family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Developmental stage	Early protein
Gene Ontology (GO)
Biological process	response to external stimulus Inferred from electronic annotation. Source: InterPro
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DAXX	Q9UER7	1	EBI-1561361,EBI-77321	From a different organism.

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Note: At least five different polypeptides are generated by alternative splicing of a common mRNA precursor.

Isoform E1B-496R (identifier: P03243-1)

Also known as: E1B-55K;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform E1B-156R (identifier: P03243-2)

Also known as: E1B-18K;

The sequence of this isoform differs from the canonical sequence as follows:
     80-419: Missing.

Isoform E1B-93R (identifier: P03243-3)

Also known as: E1B-16K;

The sequence of this isoform differs from the canonical sequence as follows:
     80-93: VAELYPELRRILTI → EGGVPTLPMQFESH
     94-495: Missing.

Isoform E1B-84R (identifier: P03243-4)

Also known as: E1B-15K;

The sequence of this isoform differs from the canonical sequence as follows:
     80-84: VAELY → QPPPP
     85-496: Missing.

Isoform E1B-176R (identifier: P03246-1)

Also known as: E1B-19K;

The sequence of this isoform can be found in the external entry P03246-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	E1B protein, large T-antigen		PRO_0000221725
Regions
	Compositional bias	47 – 51	5	Poly-Ala
	Compositional bias	56 – 61	6	Poly-Ala
Natural variations
	Alternative sequence	80 – 419	340	Missing in isoform E1B-156R.		VSP_033960
	Alternative sequence	80 – 93	14	VAELY…RILTI → EGGVPTLPMQFESH in isoform E1B-93R.		VSP_033961
	Alternative sequence	80 – 84	5	VAELY → QPPPP in isoform E1B-84R.		VSP_033962
	Alternative sequence	85 – 496	412	Missing in isoform E1B-84R.		VSP_033963
	Alternative sequence	94 – 495	402	Missing in isoform E1B-93R.		VSP_033964

Sequences

	Sequence		Length	Mass (Da)
	Isoform E1B-496R (E1B-55K) [UniParc]. Last modified July 21, 1986. Version 1. Checksum: 31EE3E4B9762DCE8	FASTA	496	55,000
	10 20 30 40 50 60 MERRNPSERG VPAGFSGHAS VESGCETQES PATVVFRPPG DNTDGGAAAA AGGSQAAAAG 70 80 90 100 110 120 AEPMEPESRP GPSGMNVVQV AELYPELRRI LTITEDGQGL KGVKRERGAC EATEEARNLA 130 140 150 160 170 180 FSLMTRHRPE CITFQQIKDN CANELDLLAQ KYSIEQLTTY WLQPGDDFEE AIRVYAKVAL 190 200 210 220 230 240 RPDCKYKISK LVNIRNCCYI SGNGAEVEID TEDRVAFRCS MINMWPGVLG MDGVVIMNVR 250 260 270 280 290 300 FTGPNFSGTV FLANTNLILH GVSFYGFNNT CVEAWTDVRV RGCAFYCCWK GVVCRPKSRA 310 320 330 340 350 360 SIKKCLFERC TLGILSEGNS RVRHNVASDC GCFMLVKSVA VIKHNMVCGN CEDRASQMLT 370 380 390 400 410 420 CSDGNCHLLK TIHVASHSRK AWPVFEHNIL TRCSLHLGNR RGVFLPYQCN LSHTKILLEP 430 440 450 460 470 480 ESMSKVNLNG VFDMTMKIWK VLRYDETRTR CRPCECGGKH IRNQPVMLDV TEELRPDHLV 490 LACTRAEFGS SDEDTD
	Isoform E1B-156R (E1B-18K). Checksum: F9517CEFCEC9DC2D	FASTA	156	16,649
	10 20 30 40 50 60 MERRNPSERG VPAGFSGHAS VESGCETQES PATVVFRPPG DNTDGGAAAA AGGSQAAAAG 70 80 90 100 110 120 AEPMEPESRP GPSGMNVVQP ESMSKVNLNG VFDMTMKIWK VLRYDETRTR CRPCECGGKH 130 140 150 IRNQPVMLDV TEELRPDHLV LACTRAEFGS SDEDTD
	Isoform E1B-93R (E1B-16K). Checksum: 19EED86F2E3FB123	FASTA	94	9,420
	10 20 30 40 50 60 MERRNPSERG VPAGFSGHAS VESGCETQES PATVVFRPPG DNTDGGAAAA AGGSQAAAAG 70 80 90 AEPMEPESRP GPSGMNVVQE GGVPTLPMQF ESHD
	Isoform E1B-84R (E1B-15K). Checksum: 6BE10C242CDB494C	FASTA	84	8,311
	10 20 30 40 50 60 MERRNPSERG VPAGFSGHAS VESGCETQES PATVVFRPPG DNTDGGAAAA AGGSQAAAAG 70 80 AEPMEPESRP GPSGMNVVQQ PPPP
	Isoform E1B-176R (E1B-19K). See P03246.	FASTA	–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 2.2 kb E1b mRNA of human Ad12 and Ad5 codes for two tumor antigens starting at different AUG triplets." Bos J.L., Polder L.J., Bernards R., Schrier P.I., van den Elsen P.J., van der Eb A.J., van Ormondt H. Cell 27:121-131(1981) [PubMed: 7326748] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of the transforming early region E1 of adenovirus type 5 DNA." van Ormondt H., Maat J., van Beveren C.P. Gene 11:299-309(1980) [PubMed: 6260576] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The sequence of the genome of adenovirus type 5 and its comparison with the genome of adenovirus type 2." Chroboczek J., Bieber F., Jacrot B. Virology 186:280-285(1992) [PubMed: 1727603] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Structure of two spliced mRNAs from the transforming region of human subgroup C adenoviruses." Perricaudet M., Akusjaervi G., Virtanen A., Pettersson U. Nature 281:694-696(1979) [PubMed: 551290] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS E1B-496R; E1B-176R; E1B-156R; E1B-93R AND E1B-84R).
[5]	"Characterization of the 55K adenovirus type 5 E1B product and related proteins." Takayesu D., Teodoro J.G., Whalen S.G., Branton P.E. J. Gen. Virol. 75:789-798(1994) [PubMed: 8151295] [Abstract] Cited for: ALTERNATIVE SPLICING (ISOFORMS E1B-496R; E1B-176R; E1B-156R; E1B-93R AND E1B-84R).

Cross-references

Sequence databases
EMBL GenBank DDBJ	M73260 Genomic DNA. No translation available. X02996 Genomic DNA. Translation: CAA26743.1. X02996 Genomic DNA. Translation: CAB40666.1.
PIR	Q1AD55. A03809.
RefSeq	AP_000199.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-569N.
IntAct	P03243. 1 interaction.
Family and domain databases
InterPro	IPR006717. Adeno_E1B_55K_N. IPR002612. AdenoE1B_55kDa. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Pfam	PF01696. Adeno_E1B_55K. 1 hit. PF04623. Adeno_E1B_55K_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E1BL_ADE05

Accession

Primary (citable) accession number: P03243
Secondary accession number(s): Q64827

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	December 15, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)